Search tips
Search criteria 


Logo of molcellbPermissionsJournals.ASM.orgJournalMCB ArticleJournal InfoAuthorsReviewers
Published online Feb 16, 2007. doi: 10.1128/MCB.00064-07
An external file that holds a picture, illustration, etc.
Object name is zmb0080767070003.jpg Object name is zmb0080767070003.jpg
FIG. 3.
Mak11-associated complexes do not contain late pre-60S factors. (A) Proteins purified in association with Mak11-TAP from wild-type (wt) cells or cells depleted for Rlp24 or Nog1 (14 h in glucose medium) were separated on a 5 to 20% polyacrylamide gradient gel and stained with colloidal Coomassie blue. Proteins identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry, listed in Table Table2,2, are indicated. (B) The presence or absence of proteins in TEV eluates from purifications using Mak11-TAP and Rlp24-TAP was determined by immunoblotting with specific antibodies. (C) The presence of Mak11 in complexes purified using Rlp24-TAP, Nog2-TAP, and Arx1-TAP was tested by immunoblotting, with Nog1 as a positive control. (D and E) RNAs associated with Mak11 complexes were enriched in the TEV protease eluate from a TAP-Mak11 (LMA326) purification. The recovered RNAs were extracted with phenol-chloroform and tested by primer extension (D) or Northern blotting (E) with the same oligonucleotides as those used for Fig. Fig.11.
Articles from Molecular and Cellular Biology are provided here courtesy of
American Society for Microbiology (ASM)