Search tips
Search criteria 


Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. 1995 November; 69(11): 7371–7374.
PMCID: PMC189672

Isolation of a highly cytopathic lentivirus from a nondomestic cat.


A feline immunodeficiency virus-like virus (FIV-Oma) isolated from a Pallas' cat (Otocolobus manul) is highly cytopathic in CrFK cells, in contrast to the chronic, noncytolytic infection established by an FIV isolate from a domestic cat (FIV-Fca). The virions have typical lentivirus morphology, density, and magnesium-dependent reverse transcriptase activity. The major core protein is antigenically cross-reactive with that of FIV-Fca; however, FIV-Oma transcripts do not cross-hybridize with FIV-Fca. A conserved region of the FIV-Oma pol gene has 76 to 80% nucleic acid identify with the corresponding pol regions of other feline lentiviruses and 64 to 69% identity with those of human, ovine, and equine lentiviruses.

Full Text

The Full Text of this article is available as a PDF (803K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ackley CD, Yamamoto JK, Levy N, Pedersen NC, Cooper MD. Immunologic abnormalities in pathogen-free cats experimentally infected with feline immunodeficiency virus. J Virol. 1990 Nov;64(11):5652–5655. [PMC free article] [PubMed]
  • Barlough JE, Ackley CD, George JW, Levy N, Acevedo R, Moore PF, Rideout BA, Cooper MD, Pedersen NC. Acquired immune dysfunction in cats with experimentally induced feline immunodeficiency virus infection: comparison of short-term and long-term infections. J Acquir Immune Defic Syndr. 1991;4(3):219–227. [PubMed]
  • Barr MC, Pough MB, Jacobson RH, Scott FW. Comparison and interpretation of diagnostic tests for feline immunodeficiency virus infection. J Am Vet Med Assoc. 1991 Nov 15;199(10):1377–1381. [PubMed]
  • Barr SC, Bowman DD, Phillips LG, Barr MC. Trypanosoma manulis n. sp. from the Russian Pallas cat Felis manul. J Eukaryot Microbiol. 1993 May-Jun;40(3):233–237. [PubMed]
  • Brown EW, Yuhki N, Packer C, O'Brien SJ. A lion lentivirus related to feline immunodeficiency virus: epidemiologic and phylogenetic aspects. J Virol. 1994 Sep;68(9):5953–5968. [PMC free article] [PubMed]
  • Campbell BJ, Thompson DR, Williams JR, Campbell SG, Avery RJ. Characterization of a New York ovine lentivirus isolate. J Gen Virol. 1993 Feb;74(Pt 2):201–210. [PubMed]
  • Carlson JR, Yee J, Hinrichs SH, Bryant ML, Gardner MB, Pedersen NC. Comparison of indirect immunofluorescence and Western blot for detection of anti-human immunodeficiency virus antibodies. J Clin Microbiol. 1987 Mar;25(3):494–497. [PMC free article] [PubMed]
  • Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. [PubMed]
  • Dow SW, Poss ML, Hoover EA. Feline immunodeficiency virus: a neurotropic lentivirus. J Acquir Immune Defic Syndr. 1990;3(7):658–668. [PubMed]
  • English RV, Nelson P, Johnson CM, Nasisse M, Tompkins WA, Tompkins MB. Development of clinical disease in cats experimentally infected with feline immunodeficiency virus. J Infect Dis. 1994 Sep;170(3):543–552. [PubMed]
  • Fomsgaard A, Hirsch VM, Allan JS, Johnson PR. A highly divergent proviral DNA clone of SIV from a distinct species of African green monkey. Virology. 1991 May;182(1):397–402. [PubMed]
  • Gelman IH, Zhang J, Hailman E, Hanafusa H, Morse SS. Identification and evaluation of new primer sets for the detection of lentivirus proviral DNA. AIDS Res Hum Retroviruses. 1992 Dec;8(12):1981–1989. [PubMed]
  • Hahn BH, Shaw GM, Arya SK, Popovic M, Gallo RC, Wong-Staal F. Molecular cloning and characterization of the HTLV-III virus associated with AIDS. Nature. 1984 Nov 8;312(5990):166–169. [PubMed]
  • Heine CW, Kelly DC, Avery RJ. The detection of intracellular retrovirus-like entities in Drosophila melanogaster cell cultures. J Gen Virol. 1980 Aug;49(2):385–395. [PubMed]
  • Ishida T, Tomoda I. Clinical staging of feline immunodeficiency virus infection. Nihon Juigaku Zasshi. 1990 Jun;52(3):645–648. [PubMed]
  • Kükedi A, Bartha A, Nagy B. Latent infection with feline syncytial virus of cell cultures prepared from the kidneys of new-born kittens. Vet Microbiol. 1988 Jan;16(1):9–14. [PubMed]
  • Langley RJ, Hirsch VM, O'Brien SJ, Adger-Johnson D, Goeken RM, Olmsted RA. Nucleotide sequence analysis of puma lentivirus (PLV-14): genomic organization and relationship to other lentiviruses. Virology. 1994 Aug 1;202(2):853–864. [PubMed]
  • Levy JA. Pathogenesis of human immunodeficiency virus infection. Microbiol Rev. 1993 Mar;57(1):183–289. [PMC free article] [PubMed]
  • Lutz H, Isenbügel E, Lehmann R, Sabapara RH, Wolfensberger C. Retrovirus infections in non-domestic felids: serological studies and attempts to isolate a lentivirus. Vet Immunol Immunopathol. 1992 Dec;35(1-2):215–224. [PubMed]
  • Miyazawa T, Fukasawa M, Hasegawa A, Maki N, Ikuta K, Takahashi E, Hayami M, Mikami T. Molecular cloning of a novel isolate of feline immunodeficiency virus biologically and genetically different from the original U.S. isolate. J Virol. 1991 Mar;65(3):1572–1577. [PMC free article] [PubMed]
  • Miyazawa T, Tomonaga K, Kawaguchi Y, Mikami T. The genome of feline immunodeficiency virus. Arch Virol. 1994;134(3-4):221–234. [PubMed]
  • O'Connor TP, Jr, Tanguay S, Steinman R, Smith R, Barr MC, Yamamoto JK, Pedersen NC, Andersen PR, Tonelli QJ. Development and evaluation of immunoassay for detection of antibodies to the feline T-lymphotropic lentivirus (feline immunodeficiency virus). J Clin Microbiol. 1989 Mar;27(3):474–479. [PMC free article] [PubMed]
  • Olmsted RA, Barnes AK, Yamamoto JK, Hirsch VM, Purcell RH, Johnson PR. Molecular cloning of feline immunodeficiency virus. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2448–2452. [PubMed]
  • Olmsted RA, Langley R, Roelke ME, Goeken RM, Adger-Johnson D, Goff JP, Albert JP, Packer C, Laurenson MK, Caro TM, et al. Worldwide prevalence of lentivirus infection in wild feline species: epidemiologic and phylogenetic aspects. J Virol. 1992 Oct;66(10):6008–6018. [PMC free article] [PubMed]
  • Oram JD, Downing RG, Roff M, Clegg JC, Serwadda D, Carswell JW. Nucleotide sequence of a Ugandan HIV-1 provirus reveals genetic diversity from other HIV-1 isolates. AIDS Res Hum Retroviruses. 1990 Sep;6(9):1073–1078. [PubMed]
  • Pedersen NC, Ho EW, Brown ML, Yamamoto JK. Isolation of a T-lymphotropic virus from domestic cats with an immunodeficiency-like syndrome. Science. 1987 Feb 13;235(4790):790–793. [PubMed]
  • Phillips TR, Talbott RL, Lamont C, Muir S, Lovelace K, Elder JH. Comparison of two host cell range variants of feline immunodeficiency virus. J Virol. 1990 Oct;64(10):4605–4613. [PMC free article] [PubMed]
  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. [PubMed]
  • Shelton GH, Grant CK, Cotter SM, Gardner MB, Hardy WD, Jr, DiGiacomo RF. Feline immunodeficiency virus and feline leukemia virus infections and their relationships to lymphoid malignancies in cats: a retrospective study (1968-1988). J Acquir Immune Defic Syndr. 1990;3(6):623–630. [PubMed]
  • Siebelink KH, Chu IH, Rimmelzwaan GF, Weijer K, van Herwijnen R, Knell P, Egberink HF, Bosch ML, Osterhaus AD. Feline immunodeficiency virus (FIV) infection in the cat as a model for HIV infection in man: FIV-induced impairment of immune function. AIDS Res Hum Retroviruses. 1990 Dec;6(12):1373–1378. [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PubMed]
  • Yamamoto JK, Pedersen NC, Ho EW, Okuda T, Theilen GH. Feline immunodeficiency syndrome--a comparison between feline T-lymphotropic lentivirus and feline leukemia virus. Leukemia. 1988 Dec;2(12 Suppl):204S–215S. [PubMed]
  • Yamamoto JK, Sparger E, Ho EW, Andersen PR, O'Connor TP, Mandell CP, Lowenstine L, Munn R, Pedersen NC. Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats. Am J Vet Res. 1988 Aug;49(8):1246–1258. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)