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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Dev Biol. Author manuscript; available in PMC 2007 June 6.
Published in final edited form as:
Dev Biol. 2007 April 1; 304(1): 246–259.
Published online 2006 December 15. doi: 10.1016/j.ydbio.2006.12.026

Fig. 8

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RT-PCR analysis of gene expression in satellite cells sorted from hindlimb and diaphragm muscles based on NES-GFP expression. Sorts from Sca1-GFP hindlimb muscle, which served as control for CD31 gene expression, yielded 2.16% GFP events (A). Sorts for NES-GFP hindlimb and diaphragm muscles yielded similar GFP events (Fig. 6). Reactions were performed using cDNA prepared from GFP positive (+) and negative (−) sorted cells, and each reaction included a positive control (con). Cells were prepared from hindlimb muscle of NES-GFP and Sca1-GFP mice (B and D) or NES-GFP diaphragm (C). Total brain RNA was used for each control reaction except for Myf5, MyoD, GFP, and c-met reactions that used total RNA isolated from primary myogenic cultures grown for 5 days.

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