RT-PCR analysis of gene expression in satellite cells sorted from hindlimb and diaphragm muscles based on NES-GFP expression. Sorts from Sca1-GFP hindlimb muscle, which served as control for CD31 gene expression, yielded 2.16% GFP events (A). Sorts for NES-GFP hindlimb and diaphragm muscles yielded similar GFP events (Fig. 6). Reactions were performed using cDNA prepared from GFP positive (+) and negative (−) sorted cells, and each reaction included a positive control (con). Cells were prepared from hindlimb muscle of NES-GFP and Sca1-GFP mice (B and D) or NES-GFP diaphragm (C). Total brain RNA was used for each control reaction except for Myf5, MyoD, GFP, and c-met reactions that used total RNA isolated from primary myogenic cultures grown for 5 days.