Scatter plots depicting fluorescence activated cell sorting of cells dissociated from NES-GFP hindlimb muscles. GFP fluorescence plotted against red autofluorescence depicts gating of the most intense GFP positive events (R1) versus negative (R2) (A and B). Sorts from NES-GFP hindlimb yielded 4% GFP events of the total (A), while wildtype hindlimb yielded no GFP fluorescence (B) as shown in the R1 gate. A similar number of negative events were collected for NES-GFP and wildtype muscles as shown by R2 gate (29–32%). Hoechst 33342 plotted against GFP depicts dual Hoechst and GFP-labeled cells in NES-GFP sort, while no GFP fluorescence is detected in the wild-type control as shown by R4 gate (A’ and B’).