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Antimicrob Agents Chemother. 1993 June; 37(6): 1232–1237.
PMCID: PMC187945

Experimental study of transplacental passage of alpha interferon by two assay techniques.


Two methods of assaying alpha interferon (IFN-alpha) were compared during an experiment aimed at determining whether IFN-alpha crosses the human placenta. Human placentas, collected after delivery following a normal pregnancy to term, were catheterized on both sides: fetal and maternal. The IFN-alpha was introduced in known amounts in the maternal circulation and was assayed in the efferent fetal fluid. The following two detection methods were used: radioimmunoassay by competition with [125I]IFN-alpha and assay with a biological system in which IFN-alpha protected Madin-Darby bovine kidney cells from destruction by vesicular stomatitis virus. The results obtained by the two methods were in perfect agreement for the efferent fetal fluid samples. They showed the absence of placental transfer of IFN-alpha. The biological method was found to be more sensitive than radioimmunoassay for low IFN-alpha titers (< 10 IU/ml) but was less reproducible, probably owing to the use of twofold dilutions. The specificities of the two methods were similar and their practicalities were equivalent; the biological method, however, was less costly. The study illustrates the complementarity of the two methods, which were based on different principles. The agreement obtained between the two methods provides a clear confirmation of the experimental results.

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Selected References

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  • Bergeret M, Grégoire A, Chany C. Protective effect of interferon on target cells exposed to cellular cytotoxicity. Immunology. 1980 Aug;40(4):637–643. [PubMed]
  • Chard T. Interferon in pregnancy. J Dev Physiol. 1989 May;11(5):271–276. [PubMed]
  • Giroux M, Campistron G, Faure F, Dumas JC, Coulais Y, Desprats R, Grandjean H, Houin G, Pontonnier G. Effects of pH on antipyrine transfer across the human placenta ex vivo. Dev Pharmacol Ther. 1990;14(3):153–160. [PubMed]
  • Gresser I, Bandu MT, Brouty-boye D, Tovey M. Pronounced antiviral activity of human interferon on bovine and porcine cells. Nature. 1974 Oct 11;251(5475):543–545. [PubMed]
  • Hartshorn KL, Vogt MW, Chou TC, Blumberg RS, Byington R, Schooley RT, Hirsch MS. Synergistic inhibition of human immunodeficiency virus in vitro by azidothymidine and recombinant alpha A interferon. Antimicrob Agents Chemother. 1987 Feb;31(2):168–172. [PMC free article] [PubMed]
  • Hira SK, Kamanga J, Bhat GJ, Mwale C, Tembo G, Luo N, Perine PL. Perinatal transmission of HIV-I in Zambia. BMJ. 1989 Nov 18;299(6710):1250–1252. [PMC free article] [PubMed]
  • Johnson VA, Barlow MA, Merrill DP, Chou TC, Hirsch MS. Three-drug synergistic inhibition of HIV-1 replication in vitro by zidovudine, recombinant soluble CD4, and recombinant interferon-alpha A. J Infect Dis. 1990 Jun;161(6):1059–1067. [PubMed]
  • Lebon P, Commoy-Chevalier MJ, Robert-Galliot B, Morin A, Chany C. Production d'interféron humain de type I par des lymphocytes au contact de cellules infectées par le virus herpès et fixées par le glutaraldéhyde. C R Seances Acad Sci D. 1980 Jan 7;290(1):37–40. [PubMed]
  • Lebon P, Ponsot G, Aicardi J, Goutières F, Arthuis M. Early intrathecal synthesis of interferon in herpes encephalitis. Biomedicine. 1979 Dec;31(9-10):267–271. [PubMed]
  • L'Haridon RM. Immunosorbent binding bioassay: a solid phase biological immunoassay for the titration of antisera to alpha interferons. J Immunoassay. 1991;12(1):99–112. [PubMed]
  • Liebes L, Mendoza S, Wilson D, Dancis J. Transfer of zidovudine (AZT) by human placenta. J Infect Dis. 1990 Feb;161(2):203–207. [PubMed]
  • Lopez-Anaya A, Unadkat JD, Schumann LA, Smith AL. Pharmacokinetics of zidovudine (azidothymidine). I. Transplacental transfer. J Acquir Immune Defic Syndr. 1990;3(10):959–964. [PubMed]
  • Newell ML, Peckham CS, Lepage P. HIV-1 infection in pregnancy: implications for women and children. AIDS. 1990;4 (Suppl 1):S111–S117. [PubMed]
  • Secher DS. Immunoradiometric assay of human leukocyte interferon using monoclonal antibody. Nature. 1981 Apr 9;290(5806):501–503. [PubMed]

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