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Antimicrob Agents Chemother. 1983 August; 24(2): 181–185.
PMCID: PMC185134

Sodium hypochlorite decontamination of split-thickness cadaveric skin infected with bacteria and yeast with subsequent isolation and growth of basal cells to confluency in tissue culture.

Abstract

The ability of sodium hypochlorite to decontaminate skin while leaving sufficient epidermal cell viability for growth in tissue culture was investigated with an in vitro system. Split-thickness cadaveric skin was infected with Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans and subsequently treated with various concentrations of sodium hypochlorite for various time intervals. Exposure to a 0.5% solution of sodium hypochlorite for 6 min effectively decontaminated the skin while leaving 66% of the basal cells viable. The basal cells were subsequently grown to confluency in tissue culture. This study demonstrates that microbial colonization of skin can be eliminated by exposure to dilute hypochlorite. This procedure, while decontaminating the skin, leaves sufficient viability of epidermal cells for subsequent growth and expansion in tissue culture, elements essential for grafting over wounds.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Berggren RB, Lehr HB. Clinical use of viable frozen human skin. JAMA. 1965 Oct 11;194(2):149–151. [PubMed]
  • BROMBERG BE, SONG IC, MOHN MP. THE USE OF PIG SKIN AS A TEMPORARY BIOLOGICAL DRESSING. Plast Reconstr Surg. 1965 Jul;36:80–90. [PubMed]
  • Eisinger M, Lee JS, Hefton JM, Darzynkiewicz Z, Chiao JW, de Harven E. Human epidermal cell cultures: growth and differentiation in the absence of differentiation in the absence of dermal components or medium supplements. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5340–5344. [PubMed]
  • Guldalian J, Jelenko C, 3rd, Callaway D, McKnight JT. A comparative study of synthetic and biological materials for wound dressings. J Trauma. 1973 Jan;13(1):32–35. [PubMed]
  • MADIN SH, DARBY NB., Jr Established kidney cell lines of normal adult bovine and ovine origin. Proc Soc Exp Biol Med. 1958 Jul;98(3):574–576. [PubMed]
  • Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975 Nov;6(3):331–343. [PubMed]
  • Saymen DG, Nathan P, Holder IA, Hill EO, Macmillan BG. Control of surface wound infection: skin versus synthetic grafts. Appl Microbiol. 1973 Jun;25(6):921–934. [PMC free article] [PubMed]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)