PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
 
Appl Environ Microbiol. 1993 June; 59(6): 1855–1863.
PMCID: PMC182172

Effects of Kraft Pulp and Lignin on Trametes versicolor Carbon Metabolism

Abstract

The white rot basidiomycete Trametes (Coriolus) versicolor can substantially increase the brightness and decrease the lignin content of washed, unbleached hardwood kraft pulp (HWKP). Monokaryotic strain 52J was used to study how HWKP and the lignin in HWKP affect the carbon metabolism and secretions of T. versicolor. Earlier work indicated that a biobleaching culture supernatant contained all components necessary for HWKP biobleaching and delignification, but the supernatant needed frequent contact with the fungus to maintain these activities. Thus, labile small fungal metabolites may be the vital biobleaching system components renewed or replaced by the fungus. Nearly all of the CO2 evolved by HWKP-containing cultures came from the added glucose, indicating that HWKP is not an important source of carbon or energy during biobleaching. Carbon dioxide appeared somewhat earlier in the absence of HWKP, but the culture partial O2 pressure was little affected by the presence of pulp. The presence of HWKP in a culture markedly increased the culture's production of a number of acidic metabolites, including 2-phenyllactate, oxalate, adipate, glyoxylate, fumarate, mandelate, and glycolate. Although the total concentration of these pulp-induced metabolites was only 4.3 mM, these compounds functioned as effective manganese-complexing agents for the manganese peroxidase-mediated oxidation of phenol red, propelling the reaction at 2.4 times the rate of 50 mM sodium malonate, the standard chelator-buffer. The presence of HWKP in a culture also markedly stimulated fungal secretion of the enzymes manganese peroxidase, cellulase, and cellobiose-quinone oxidoreductase, but not laccase (phenol oxidase) or lignin peroxidase.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Addleman K, Archibald F. Kraft Pulp Bleaching and Delignification by Dikaryons and Monokaryons of Trametes versicolor. Appl Environ Microbiol. 1993 Jan;59(1):266–273. [PMC free article] [PubMed]
  • Archibald FS. Lignin Peroxidase Activity Is Not Important in Biological Bleaching and Delignification of Unbleached Kraft Pulp by Trametes versicolor. Appl Environ Microbiol. 1992 Sep;58(9):3101–3109. [PMC free article] [PubMed]
  • Archibald F, Roy B. Production of manganic chelates by laccase from the lignin-degrading fungus Trametes (Coriolus) versicolor. Appl Environ Microbiol. 1992 May;58(5):1496–1499. [PMC free article] [PubMed]
  • Bourbonnais R, Paice MG. Veratryl alcohol oxidases from the lignin-degrading basidiomycete Pleurotus sajor-caju. Biochem J. 1988 Oct 15;255(2):445–450. [PubMed]
  • Fåhraeus G, Reinhammar B. Large scale production and purification of laccase from cultures of the fungus Polyporus versicolor and some properties of laccase A. Acta Chem Scand. 1967;21(9):2367–2378. [PubMed]
  • Glenn JK, Morgan MA, Mayfield MB, Kuwahara M, Gold MH. An extracellular H2O2-requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete Phanerochaete chrysosporium. Biochem Biophys Res Commun. 1983 Aug 12;114(3):1077–1083. [PubMed]
  • Kelley RL, Reddy CA. Purification and characterization of glucose oxidase from ligninolytic cultures of Phanerochaete chrysosporium. J Bacteriol. 1986 Apr;166(1):269–274. [PMC free article] [PubMed]
  • Kersten PJ, Kirk TK. Involvement of a new enzyme, glyoxal oxidase, in extracellular H2O2 production by Phanerochaete chrysosporium. J Bacteriol. 1987 May;169(5):2195–2201. [PMC free article] [PubMed]
  • Keyser P, Kirk TK, Zeikus JG. Ligninolytic enzyme system of Phanaerochaete chrysosporium: synthesized in the absence of lignin in response to nitrogen starvation. J Bacteriol. 1978 Sep;135(3):790–797. [PMC free article] [PubMed]
  • Kirkpatrick N, Reid ID, Ziomek E, Ho C, Paice MG. Relationship between Fungal Biomass Production and the Brightening of Hardwood Kraft Pulp by Coriolus versicolor. Appl Environ Microbiol. 1989 May;55(5):1147–1152. [PMC free article] [PubMed]
  • Lackner R, Srebotnik E, Messner K. Oxidative degradation of high molecular weight chlorolignin by manganese peroxidase of Phanerochaete chrysosporium. Biochem Biophys Res Commun. 1991 Aug 15;178(3):1092–1098. [PubMed]
  • Lingappa BT, Prasad M, Lingappa Y, Hunt DF, Biemann K. Phenethyl alcohol and tryptophol: autoantibiotics produced by the fungus Candida albicans. Science. 1969 Jan 10;163(3863):192–194. [PubMed]
  • Morpeth FF. Some properties of cellobiose oxidase from the white-rot fungus Sporotrichum pulverulentum. Biochem J. 1985 Jun 15;228(3):557–564. [PubMed]
  • Morpeth FF, Jones GD. Resolution, purification and some properties of the multiple forms of cellobiose quinone dehydrogenase from the white-rot fungus Sporotrichum pulverulentum. Biochem J. 1986 May 15;236(1):221–226. [PubMed]
  • Narayanan TK, Rao GR. Production of 2-phenethyl alcohol and 2-phenyllactic acid in Candida species. Biochem Biophys Res Commun. 1974 Jun 4;58(3):728–735. [PubMed]
  • Narayanan TK, Rao GR. Production of beta-(4-hydroxyphenyl)ethanol and beta-(4-hydroxyphenyl)lactic acid by Candida species. Can J Microbiol. 1976 Mar;22(3):384–389. [PubMed]
  • Paice MG, Reid ID, Bourbonnais R, Archibald FS, Jurasek L. Manganese Peroxidase, Produced by Trametes versicolor during Pulp Bleaching, Demethylates and Delignifies Kraft Pulp. Appl Environ Microbiol. 1993 Jan;59(1):260–265. [PMC free article] [PubMed]
  • Klyosov AA. Trends in biochemistry and enzymology of cellulose degradation. Biochemistry. 1990 Nov 27;29(47):10577–10585. [PubMed]
  • Samejima M, Eriksson KE. Mechanisms of redox interactions between lignin peroxidase and cellobiose:quinone oxidoreductase. FEBS Lett. 1991 Nov 4;292(1-2):151–153. [PubMed]
  • Scott W, Foote JL. The inhibition of stearoyl-coenzyme A desaturase by phenyllactate and phenylpyruvate. Biochim Biophys Acta. 1979 Apr 27;573(1):197–200. [PubMed]
  • Tien M, Kirk TK. Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds. Science. 1983 Aug 12;221(4611):661–663. [PubMed]
  • Wariishi H, Dunford HB, MacDonald ID, Gold MH. Manganese peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Transient state kinetics and reaction mechanism. J Biol Chem. 1989 Feb 25;264(6):3335–3340. [PubMed]
  • Wariishi H, Valli K, Renganathan V, Gold MH. Thiol-mediated oxidation of nonphenolic lignin model compounds by manganese peroxidase of Phanerochaete chrysosporium. J Biol Chem. 1989 Aug 25;264(24):14185–14191. [PubMed]
  • Yu EK, Saddler JN. Enhanced Production of 2,3-Butanediol by Klebsiella pneumoniae Grown on High Sugar Concentrations in the Presence of Acetic Acid. Appl Environ Microbiol. 1982 Oct;44(4):777–784. [PMC free article] [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)