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J Bacteriol. 1997 April; 179(8): 2753–2756.
PMCID: PMC179027

Characterization of large linear plasmids in mycobacteria.


Linear plasmids were found in Mycobacterium xenopi, M. branderi, and M. celatum. These elements represented a wide size range (from 20 to 320 kb), had Streptomyces-like terminal structures, and defined five hybridization groups. Cross-hybridization and common restriction fragment length polymorphism patterns of plasmids from the different species suggest either genetic exchange or a common ancestry of the species.

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Selected References

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  • Baess I. Determination and re-examination of genome sizes and base ratios on deoxyribonucleic acid from mycobacteria. Acta Pathol Microbiol Immunol Scand B. 1984 Aug;92(4):209–211. [PubMed]
  • Barbour AG, Garon CF. Linear plasmids of the bacterium Borrelia burgdorferi have covalently closed ends. Science. 1987 Jul 24;237(4813):409–411. [PubMed]
  • Beverley SM. Characterization of the 'unusual' mobility of large circular DNAs in pulsed field-gradient electrophoresis. Nucleic Acids Res. 1988 Feb 11;16(3):925–939. [PMC free article] [PubMed]
  • Bull TJ, Shanson DC, Archard LC, Yates MD, Hamid ME, Minnikin DE. A new group (type 3) of Mycobacterium celatum isolated from AIDS patients in the London area. Int J Syst Bacteriol. 1995 Oct;45(4):861–862. [PubMed]
  • Butler WR, O'Connor SP, Yakrus MA, Smithwick RW, Plikaytis BB, Moss CW, Floyd MM, Woodley CL, Kilburn JO, Vadney FS, et al. Mycobacterium celatum sp. nov. Int J Syst Bacteriol. 1993 Jul;43(3):539–548. [PubMed]
  • Chen CW, Yu TW, Lin YS, Kieser HM, Hopwood DA. The conjugative plasmid SLP2 of Streptomyces lividans is a 50 kb linear molecule. Mol Microbiol. 1993 Mar;7(6):925–932. [PubMed]
  • Crawford JT, Bates JH. Isolation of plasmids from mycobacteria. Infect Immun. 1979 Jun;24(3):979–981. [PMC free article] [PubMed]
  • Crawford JT, Bates JH. Restriction endonuclease mapping and cloning of Mycobacterium intracellulare plasmid pLR7. Gene. 1984 Mar;27(3):331–333. [PubMed]
  • Crawford JT, Cave MD, Bates JH. Evidence for plasmid-mediated restriction-modification in Mycobacterium avium intracellulare. J Gen Microbiol. 1981 Dec;127(2):333–338. [PubMed]
  • Crespi M, Messens E, Caplan AB, van Montagu M, Desomer J. Fasciation induction by the phytopathogen Rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene. EMBO J. 1992 Mar;11(3):795–804. [PubMed]
  • Embley TM, Stackebrandt E. The molecular phylogeny and systematics of the actinomycetes. Annu Rev Microbiol. 1994;48:257–289. [PubMed]
  • Erardi FX, Failla ML, Falkinham JO., 3rd Plasmid-encoded copper resistance and precipitation by Mycobacterium scrofulaceum. Appl Environ Microbiol. 1987 Aug;53(8):1951–1954. [PMC free article] [PubMed]
  • Estrada-G IC, Colston MJ, Cox RA. Determination and evolutionary significance of nucleotide sequences near to the 3'-end of 16S ribosomal RNA of mycobacteria. FEMS Microbiol Lett. 1989 Oct 15;52(3):285–290. [PubMed]
  • Ferdows MS, Barbour AG. Megabase-sized linear DNA in the bacterium Borrelia burgdorferi, the Lyme disease agent. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5969–5973. [PubMed]
  • Hinnebusch J, Tilly K. Linear plasmids and chromosomes in bacteria. Mol Microbiol. 1993 Dec;10(5):917–922. [PubMed]
  • Hull SI, Wallace RJ, Jr, Bobey DG, Price KE, Goodhines RA, Swenson JM, Silcox VA. Presence of aminoglycoside acetyltransferase and plasmids in Mycobacterium fortuitum. Lack of correlation with intrinsic aminoglycoside resistance. Am Rev Respir Dis. 1984 Apr;129(4):614–618. [PubMed]
  • Jensen AG, Bennedsen J, Rosdahl VT. Plasmid profiles of mycobacterium avium/intracellulare isolated from patients with AIDS or cervical lymphadenitis and from environmental samples. Scand J Infect Dis. 1989;21(6):645–649. [PubMed]
  • Jucker MT, Falkinham JO., 3rd Epidemiology of infection by nontuberculous mycobacteria IX. Evidence for two DNA homology groups among small plasmids in Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum. Am Rev Respir Dis. 1990 Oct;142(4):858–862. [PubMed]
  • Kalkus J, Reh M, Schlegel HG. Hydrogen autotrophy of Nocardia opaca strains is encoded by linear megaplasmids. J Gen Microbiol. 1990 Jun;136(6):1145–1151. [PubMed]
  • Komeda H, Kobayashi M, Shimizu S. Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1. Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4267–4272. [PubMed]
  • Koukila-Kähkölä P, Springer B, Böttger EC, Paulin L, Jantzen E, Katila ML. Mycobacterium branderi sp. nov., a new potential human pathogen. Int J Syst Bacteriol. 1995 Jul;45(3):549–553. [PubMed]
  • Levene SD, Zimm BH. Separations of open-circular DNA using pulsed-field electrophoresis. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4054–4057. [PubMed]
  • Meissner PS, Falkinham JO., 3rd Plasmid-encoded mercuric reductase in Mycobacterium scrofulaceum. J Bacteriol. 1984 Feb;157(2):669–672. [PMC free article] [PubMed]
  • Philipp WJ, Poulet S, Eiglmeier K, Pascopella L, Balasubramanian V, Heym B, Bergh S, Bloom BR, Jacobs WR, Jr, Cole ST. An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae. Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):3132–3137. [PubMed]
  • Picardeau M, Varnerot A, Rauzier J, Gicquel B, Vincent V. Mycobacterium xenopi IS1395, a novel insertion sequence expanding the IS256 family. Microbiology. 1996 Sep;142(Pt 9):2453–2461. [PubMed]
  • Rauzier J, Moniz-Pereira J, Gicquel-Sanzey B. Complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum. Gene. 1988 Nov 30;71(2):315–321. [PubMed]
  • Saint Girons I, Old IG, Davidson BE. Molecular biology of the Borrelia, bacteria with linear replicons. Microbiology. 1994 Aug;140(Pt 8):1803–1816. [PubMed]

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