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J Bacteriol. 1997 February; 179(4): 998–1006.
PMCID: PMC178790

A role for cpeYZ in cyanobacterial phycoerythrin biosynthesis.


Pigment mutant strain FdR1 of the filamentous cyanobacterium Fremyella diplosiphon is characterized by constitutive synthesis of the phycobiliprotein phycoerythrin due to insertional inactivation of the rcaC regulatory gene by endogenous transposon Tn5469. Whereas the parental strain Fd33 harbors five genomic copies of Tn5469, cells of strain FdR1 harbor six genomic copies of the element; the sixth copy in FdR1 is localized to the rcaC gene. Electroporation of FdR1 cells yielded secondary pigment mutant strains FdR1E1 and FdR1E4, which identically exhibited the FdR1 phenotype with significantly reduced levels of phycoerythrin. In both FdR1E1 and FdR1E4, a seventh genomic copy of Tn5469 was localized to the cpeY gene of the sequenced but phenotypically uncharacterized cpeYZ gene set. This gene set is located downstream of the cpeBA operon which encodes the alpha and beta subunits of phycoerythrin. Complementation experiments correlated cpeYZ activity to the phenotype of strains FdR1E1 and FdR1E4. The predicted CpeY and CpeZ proteins share significant sequence identity with the products of homologous cpeY and cpeZ genes reported for Pseudanabaena sp. strain PCC 7409 and Synechococcus sp. strain WH 8020, both of which synthesize phycoerythrin. The CpeY and CpeZ proteins belong to a family of structurally related cyanobacterial proteins that includes the subunits of the CpcE/CpcF phycocyanin alpha-subunit lyase of Synechococcus sp. strain PCC 7002 and the subunits of the PecE/PecF phycoerythrocyanin alpha-subunit lyase of Anabaena sp. strain PCC 7120. Phycobilisomes isolated from mutant strains FdR1E1 and FdR1E4 contained equal amounts of chromophorylated alpha and beta subunits of phycoerythrin at 46% of the levels of the parental strain FdR1. These results suggest that the cpeYZ gene products function in phycoerythrin synthesis, possibly as a lyase involved in the attachment of phycoerythrobilin to the alpha or beta subunit.

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Selected References

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  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Belknap WR, Haselkorn R. Cloning and light regulation of expression of the phycocyanin operon of the cyanobacterium Anabaena. EMBO J. 1987 Apr;6(4):871–884. [PubMed]
  • Bruns BU, Briggs WR, Grossman AR. Molecular characterization of phycobilisome regulatory mutants of Fremyella diplosiphon. J Bacteriol. 1989 Feb;171(2):901–908. [PMC free article] [PubMed]
  • Bryant DA, Stirewalt VL, Glauser M, Frank G, Sidler W, Zuber H. A small multigene family encodes the rod-core linker polypeptides of Anabaena sp. PCC7120 phycobilisomes. Gene. 1991 Oct 30;107(1):91–99. [PubMed]
  • Chiang GG, Schaefer MR, Grossman AR. Complementation of a red-light-indifferent cyanobacterial mutant. Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9415–9419. [PubMed]
  • Cobley JG, Zerweck E, Reyes R, Mody A, Seludo-Unson JR, Jaeger H, Weerasuriya S, Navankasattusas S. Construction of shuttle plasmids which can be efficiently mobilized from Escherichia coli into the chromatically adapting cyanobacterium, Fremyella diplosiphon. Plasmid. 1993 Sep;30(2):90–105. [PubMed]
  • Dubbs JM, Bryant DA. Molecular cloning and transcriptional analysis of the cpeBA operon of the cyanobacterium Pseudanabaena species PCC7409. Mol Microbiol. 1991 Dec;5(12):3073–3085. [PubMed]
  • Fairchild CD, Glazer AN. Oligomeric structure, enzyme kinetics, and substrate specificity of the phycocyanin alpha subunit phycocyanobilin lyase. J Biol Chem. 1994 Mar 25;269(12):8686–8694. [PubMed]
  • Fairchild CD, Zhao J, Zhou J, Colson SE, Bryant DA, Glazer AN. Phycocyanin alpha-subunit phycocyanobilin lyase. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7017–7021. [PubMed]
  • Glauser M, Stirewalt VL, Bryant DA, Sidler W, Zuber H. Structure of the genes encoding the rod-core linker polypeptides of Mastigocladus laminosus phycobilisomes and functional aspects of the phycobiliprotein/linker-polypeptide interactions. Eur J Biochem. 1992 May 1;205(3):927–937. [PubMed]
  • Grossman AR, Schaefer MR, Chiang GG, Collier JL. The phycobilisome, a light-harvesting complex responsive to environmental conditions. Microbiol Rev. 1993 Sep;57(3):725–749. [PMC free article] [PubMed]
  • Jung LJ, Chan CF, Glazer AN. Candidate genes for the phycoerythrocyanin alpha subunit lyase. Biochemical analysis of pecE and pecF interposon mutants. J Biol Chem. 1995 May 26;270(21):12877–12884. [PubMed]
  • Kahn K, Schaefer MR. Characterization of transposon Tn5469 from the cyanobacterium Fremyella diplosiphon. J Bacteriol. 1995 Dec;177(24):7026–7032. [PMC free article] [PubMed]
  • Kalla SR, Lind LK, Lidholm J, Gustafsson P. Transcriptional organization of the phycocyanin subunit gene clusters of the cyanobacterium Anacystis nidulans UTEX 625. J Bacteriol. 1988 Jul;170(7):2961–2970. [PMC free article] [PubMed]
  • Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E, Nakamura Y, Miyajima N, Hirosawa M, Sugiura M, Sasamoto S, et al. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res. 1996 Jun 30;3(3):109–136. [PubMed]
  • Ong LJ, Glazer AN. Phycoerythrins of marine unicellular cyanobacteria. I. Bilin types and locations and energy transfer pathways in Synechococcus spp. phycoerythrins. J Biol Chem. 1991 May 25;266(15):9515–9527. [PubMed]
  • Raps S. Differentiation between Phycobiliprotein and Colorless Linker Polypeptides by Fluorescence in the Presence of ZnSO(4). Plant Physiol. 1990 Feb;92(2):358–362. [PubMed]
  • Reed KC, Mann DA. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985 Oct 25;13(20):7207–7221. [PMC free article] [PubMed]
  • Swanson RV, de Lorimier R, Glazer AN. Genes encoding the phycobilisome rod substructure are clustered on the Anabaena chromosome: characterization of the phycoerythrocyanin operon. J Bacteriol. 1992 Apr;174(8):2640–2647. [PMC free article] [PubMed]
  • Swanson RV, Zhou J, Leary JA, Williams T, de Lorimier R, Bryant DA, Glazer AN. Characterization of phycocyanin produced by cpcE and cpcF mutants and identification of an intergenic suppressor of the defect in bilin attachment. J Biol Chem. 1992 Aug 15;267(23):16146–16154. [PubMed]
  • Wilbanks SM, de Lorimier R, Glazer AN. Phycoerythrins of marine unicellular cyanobacteria. III. Sequence of a class II phycoerythrin. J Biol Chem. 1991 May 25;266(15):9535–9539. [PubMed]
  • Wilbanks SM, Glazer AN. Rod structure of a phycoerythrin II-containing phycobilisome. I. Organization and sequence of the gene cluster encoding the major phycobiliprotein rod components in the genome of marine Synechococcus sp. WH8020. J Biol Chem. 1993 Jan 15;268(2):1226–1235. [PubMed]
  • Zhou J, Gasparich GE, Stirewalt VL, de Lorimier R, Bryant DA. The cpcE and cpcF genes of Synechococcus sp. PCC 7002. Construction and phenotypic characterization of interposon mutants. J Biol Chem. 1992 Aug 15;267(23):16138–16145. [PubMed]

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