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J Bacteriol. 1996 August; 178(16): 5045–5048.
PMCID: PMC178295

Escherichia coli DNA repair genes radA and sms are the same gene.


Escherichia coli strains carrying radA100 or sms mutations were identical in their sensitivities to either methyl methanesulfonate or UV radiation treatment and in their plasmid complementation patterns for UV radiation survival. DNA sequencing analysis of the radA mutant and radA+ strains and comparison of their sequences with the published sms gene sequence showed the radA mutant to differ only by a G-to-A transition mutation, which is predicted to change a cysteine in a zinc-finger motif to tyrosine. The sms gene is concluded to be identical to the previously described radA gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bachmann BJ. Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev. 1990 Jun;54(2):130–197. [PMC free article] [PubMed]
  • Berg JM. Potential metal-binding domains in nucleic acid binding proteins. Science. 1986 Apr 25;232(4749):485–487. [PubMed]
  • Diver WP, Sargentini NJ, Smith KC. A mutation (radA100) in Escherichia coli that selectively sensitizes cells grown in rich medium to x- or u.v.-radiation, or methyl methanesulphonate. Int J Radiat Biol Relat Stud Phys Chem Med. 1982 Sep;42(3):339–346. [PubMed]
  • Dutreix M, Moreau PL, Bailone A, Galibert F, Battista JR, Walker GC, Devoret R. New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis. J Bacteriol. 1989 May;171(5):2415–2423. [PMC free article] [PubMed]
  • Felzenszwalb I, Sargentini NJ, Smith KC. Characterization of a new radiation-sensitive mutant, Escherichia coli K-12 radC102. Radiat Res. 1984 Mar;97(3):615–625. [PubMed]
  • Klug A, Schwabe JW. Protein motifs 5. Zinc fingers. FASEB J. 1995 May;9(8):597–604. [PubMed]
  • Krasin F, Hutchinson F. Repair of DNA double-strand breaks in Escherichia coli, which requires recA function and the presence of a duplicate genome. J Mol Biol. 1977 Oct 15;116(1):81–98. [PubMed]
  • Neuwald AF, Berg DE, Stauffer GV. Mutational analysis of the Escherichia coli serB promoter region reveals transcriptional linkage to a downstream gene. Gene. 1992 Oct 12;120(1):1–9. [PubMed]
  • O'Connor TR, Graves RJ, de Murcia G, Castaing B, Laval J. Fpg protein of Escherichia coli is a zinc finger protein whose cysteine residues have a structural and/or functional role. J Biol Chem. 1993 Apr 25;268(12):9063–9070. [PubMed]
  • Sargentini NJ, Smith KC. Quantitation of the involvement of the recA, recB, recC, recF, recJ, recN, lexA, radA, radB, uvrD, and umuC genes in the repair of X-ray-induced DNA double-strand breaks in Escherichia coli. Radiat Res. 1986 Jul;107(1):58–72. [PubMed]
  • Sargentini NJ, Smith KC. Ionizing and ultraviolet radiation-induced reversion of sequenced frameshift mutations in Escherichia coli: a new role for umuDC suggested by delayed photoreactivation. Mutat Res. 1987 Jul;179(1):55–63. [PubMed]
  • Sargentini NJ, Smith KC. DNA sequence analysis of gamma-radiation (anoxic)-induced and spontaneous lacId mutations in Escherichia coli K-12. Mutat Res. 1994 Sep 1;309(2):147–163. [PubMed]
  • Wang J, Grossman L. Mutations in the helix-turn-helix motif of the Escherichia coli UvrA protein eliminate its specificity for UV-damaged DNA. J Biol Chem. 1993 Mar 5;268(7):5323–5331. [PubMed]

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