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Recent genetic and cell biological studies illustrate the importance of active transforming growth factor-β signaling in preventing the proliferation of estrogen receptor-positive cells in the normal mammary gland, and suggest how the loss of this inhibition may be important in early breast cancer progression.
A recent report by Ewan and colleagues  sheds some light on the mechanism by which steroid receptor-positive cells are prevented from proliferating in the normal adult mammary gland by the expression of activated transforming growth factor (TGF)-β. This report is an extension of earlier studies by the same authors that first demonstrated the ability to detect latent versus activated TGF-β expression in situ . Steroid receptor expression is often used as a prognostic indicator and target of endocrine therapy in breast cancer. However, our understanding of the normal distribution and regulation of estrogen receptor (ER)-α and progesterone receptor (PR) expression is still evolving. In normal adult mammary glands from humans, rats, mice, or cows, steroid receptor-positive cells are heterogeneously located in luminal epithelial cells throughout the ducts and rarely co-localize with markers of proliferation [3-6]. Growth factors expressed in steroid receptor-positive cells act on neighboring cells to induce proliferation in a paracrine fashion. An elegant series of experiments by Cathrin Brisken and her colleagues [7,8] have established the ability of estrogen receptor-positive or progesterone receptor-positive cells to rescue ER-null and PR-null cells, respectively, in chimeric fat-pad transplantation experiments. However, a key question in mammary gland development is what prevents ER/PR-positive cells from dividing and why this proliferative block is lost during breast cancer progression. For example, ER/PR-positive cells are often proliferative in mouse models of mammary hyperplasia and in precancerous lesions of the human breast, such as ductal carcinoma in situ, suggesting a switch from a paracrine to an autocrine response to proliferative stimuli [9,10].
TGF-β is a potent inhibitor of epithelial cell proliferation, but little is known about the process involved in activating the latent form secreted by most cells . The relationship between TGF-β activation, ER-α expression, and proliferation were the focus of the recent study by Ewan and colleagues . Using immunostaining to detect the activated form of TGF-β during estrus, they were able to show that the cells positive for active TGF-β also expressed the downstream effector R-SMAD in the nucleus, providing evidence that TGF-β acts in an autocrine manner. Co-immunostaining experiments using double immunofluorescence were performed to show that ER-α co-localized with nuclear R-SMAD staining and activated TGF-β co-localized with both ER-α and PR, supporting their hypothesis that TGF-β activation might inhibit the ability of ER/PR-expressing cells to respond to ovarian hormone-induced proliferation.
Mice heterozygous for the TGF-β1 allele have a 90% reduction in the amount of TGF-β1 protein expressed . Using mammary glands from these mice, the expression of ER-α and Ki67, a marker of proliferation, was assessed. Mammary glands from TGF-β1+/- mice exhibited a 24-fold increase in proliferation, and co-localization of ER-α and Ki67 was increased 16-fold. The same results were found when this analysis was performed on outgrowths after transplantation of TGF-β1+/- mammary epithelial cells, suggesting that epithelial, rather than stromal, TGF-β is responsible for keeping ER/PR-positive cells from proliferating. After ovariectomy, no ER-α/Ki67 double-positive cells were observed in TGF-β1+/- glands in the absence of ovarian hormones. Only when estrogen and progesterone were added back was there a 17-fold increase in double-labeled cells. Although a loss of TGF-β expression resulted in increased proliferation in the presence of ovarian hormones, the expression of constitutively active TGF-β was able to override hormone-induced proliferation. Mammary glands from mouse mammary tumor virus (MMTV)–TGF-β transgenic mice displayed sixfold fewer ER-α/Ki67 double-positive cells than wild-type glands at estrus.
Fewer proliferating ER-α-positive cells are detected in the mammary glands of parous humans or rats than in glands from nulliparous subjects [4,13]. To test whether TGF-β has a function in this phenomenon, parous glands from TGF-β1+/- mice were analyzed for ER-α and Ki67 co-localization. The frequency of double-labeled cells in parous glands from TGF-β1+/- mice was fourfold that in parous wild-type mice, indicating that TGF-β is also involved in inhibiting the proliferation of ER-α-positive cells in parous animals. These data also fit with the findings of Boulanger and colleagues, who demonstrated that TGF-β-positive cells from parous glands did not contribute to repopulating the gland, suggesting that they were incapable of 'expansive cellular proliferation' and stem cell self-renewal . TGF-β expression has also been shown to increase mammary stem cell senescence and to inhibit MMTV-induced mammary carcinogenesis . Finally, the effect of TGF-β1 depletion was analyzed during puberty, a time of rapid proliferation in the terminal end bud structures. Loss of TGF-β1 did not increase the proliferation of ER-α-positive cells in either the terminal end buds or ducts, although proliferation was increased overall. This supports the idea that proliferation of ER/PR-positive cells during puberty is regulated differently from that in the adult gland.
These data advance our understanding of how steroid receptor-positive cells might be prevented from proliferating in the normal mammary gland, and how this process might become deregulated in breast cancer progression. Further evidence for the role of TGF-β in regulating the proliferation of steroid receptor-expressing cells comes from our studies of CCAAT-enhancer-binding protein (C/EBP)β-null mice. Mammary glands from these mice show increased numbers of ER/PR-positive cells and a 10-fold decrease in proliferation . Recently, we discovered that these C/EBPβ-null glands also display a significant increase in activated TGF-β along with increased downstream Smad2 expression and signaling . Another downstream target of TGF-β is the cyclin-dependent kinase inhibitor, p27Kip1, which in turn affects the activity of other cell cycle components such as cyclin E and cyclin-dependent kinase (cdk)2 . All of these molecules were altered in C/EBPβ-null glands, resulting in decreased cyclin E expression, loss of cdk2 kinase activity, increased p27 stability and decreased levels of cdc25A phosphatase activity. These studies with mouse models have led us to speculate that loss of active TGF-β expression in precancerous breast lesions might result in increased expression or stability of cdc25A and increased cyclin E/cdk2 activity in steroid receptor-positive cells, allowing them to proliferate. In support of this hypothesis, cdc25A was recently shown to be induced by genomic and non-genomic actions of estrogen in breast cancer cells [18,19]. The expression of cdc25a is also regulated both transcriptionally and post-transcriptionally by TGF-β, and thus may be a useful downstream indicator of active TGF-β signaling . p27 has also been proposed as a prognostic marker in breast cancer . However, the subcellular localization and phosphorylation state of the protein are critical in regulating its activity, and it would, therefore, be problematic to assess these changes in clinical samples. A hypothetical model summarizing these results with regard to breast cancer progression is shown in Figure Figure11.
Critical questions that still need to be addressed are what mechanisms are responsible for the activation of TGF-β only in the ER/PR-positive cells, and how the patterning of cells expressing steroid receptors is established both in normal development and breast cancer. With the availability of appropriate immunological reagents, these hypotheses should be tested with patient samples, to determine whether these markers will provide a method for predicting which patients with ductal carcinoma in situ have a higher likelihood of progression to infiltrating ductal carcinoma.
cdk = cyclin-dependent kinase; C/EBP = CCAAT-enhancer-binding protein; ER = estrogen receptor; MMTV = mouse mammary tumor virus; PR = progesterone receptor; TGF = transforming growth factor.
The authors declare that they have no competing interests.
This study was supported by NIH grant CA16303.