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Antimicrob Agents Chemother. 1988 December; 32(12): 1854–1858.
PMCID: PMC176032

Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri.


Lactobacillus reuteri is a prominent member of the Lactobacillus population in the gastrointestinal ecosystem of humans, poultry, swine, and other animals. Reuterin is a newly discovered, broad-spectrum antimicrobial substance produced by this species during fermentation of glycerol. In this report, we describe procedures for (i) producing reuterin in sufficient amounts to isolate from a fermentation mixture and (ii) isolating this substance by high-performance liquid chromatography. By using uniformly labeled [14C]glycerol, reuterin was identified as a product of glycerol fermentation associated with the production of beta-hydroxypropionic acid and trimethylene glycol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ABELES RH, BROWNSTEIN AM, RANDLES CH. beta-Hydroxypropionaldehyde, an intermediate in the formation of 1,3-propanediol by Aerobacter aerogenes. Biochim Biophys Acta. 1960 Jul 15;41:530–531. [PubMed]
  • Axelsson L, Lindgren S. Characterization and DNA homology of Lactobacillus strains isolated from pig intestine. J Appl Bacteriol. 1987 May;62(5):433–440. [PubMed]
  • Forage RG, Lin EC. DHA system mediating aerobic and anaerobic dissimilation of glycerol in Klebsiella pneumoniae NCIB 418. J Bacteriol. 1982 Aug;151(2):591–599. [PMC free article] [PubMed]
  • Forsberg CW. Production of 1,3-Propanediol from Glycerol by Clostridium acetobutylicum and Other Clostridium Species. Appl Environ Microbiol. 1987 Apr;53(4):639–643. [PMC free article] [PubMed]
  • SOBOLOV M, SMILEY KL. Metabolism of glycerol by an acrolein-forming lactobacillus. J Bacteriol. 1960 Feb;79:261–266. [PMC free article] [PubMed]

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