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Logo of annrheumdAnnals of the Rheumatic DiseasesCurrent TOCInstructions for authors
 
Ann Rheum Dis. Dec 2002; 61(12): 1047–1054.
PMCID: PMC1753975
Receptor activator NF-κB ligand (RANKL) expression in synovial tissue from patients with rheumatoid arthritis, spondyloarthropathy, osteoarthritis, and from normal patients: semiquantitative and quantitative analysis
T Crotti, M Smith, H Weedon, M Ahern, D Findlay, M Kraan, P Tak, and D Haynes
Department of Pathology, University of Adelaide, Adelaide, South Australia.
Objectives: To compare receptor activator of NF-κB ligand (RANKL) production in the synovial tissue from patients with active rheumatoid arthritis (RA), inactive RA, spondyloarthropathies (SpA), osteoarthritis, and from normal subjects. In addition, to establish the cell lineages expressing RANKL in these tissues.
Methods: Immunohistological analysis of frozen synovial tissue biopsy specimens was performed using a monoclonal antibody (mAb) to detect RANKL. Sections were evaluated by computer assisted image analysis and semiquantitative analysis to compare RANKL expression between groups. Dual and sequential labelling with mAb RANKL and cell lineage specific monoclonal antibodies were used to determine the types of cells expressing RANKL.
Results: Higher levels of RANKL were expressed in tissues from patients with active RA and SpA than in tissues from patients with inactive RA, osteoarthritis, and from normal subjects. RANKL protein was associated with CD3 antigen-positive lymphocytes and some macrophages. RANKL was predominantly associated with activated, memory T cells (CD45Ro positive cells) in patients with active RA and spondyloarthropathy (SpA).
Conclusions: The highest levels of RANKL were detected in patients with RA with active synovitis and in some patients with SpA. An increase in RANKL in the inflamed joint of patients with RA, produced by infiltrating activated T cells and macrophages, is likely to be an important cause of joint erosions in RA.
Figure 1
Figure 1
RANKL detected with mAb 626/immunoperoxidase and AEC (red), in synovial tissue from a patient with RA with (A) active disease and (B) inactive disease, (C) a patient with SpA, and (D) a patient with OA. (E) to (H) identify cell lineages expressing RANKL (more ...)
Figure 2
Figure 2
Panels (A), (C), (E), (G) demonstrate RANKL expression, detected with mAb 626/ immunoperoxidase and AEC (red), in synovial tissue from a patient with RA with active disease (A), inactive disease (C), SpA (E), and from a normal subject (G). Panels (B), (more ...)
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