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Infect Immun. 1996 July; 64(7): 2415–2419.
PMCID: PMC174091

Differential effects of the streptococcal fibronectin-binding protein, FBP54, on adhesion of group A streptococci to human buccal cells and HEp-2 tissue culture cells.


We have previously demonstrated that fibronectin mediates streptococcal adhesion to host cells and that streptococci interact primarily with the N-terminal domain of fibronectin. FBP54 is a 54-kDa protein from group A streptococci that binds fibronectin. In this report, we show that the N-terminal domain of fibronectin reacts with FBP54 and preferentially blocks streptococcal adhesion to buccal epithelial cells. FBP54 blocked adhesion to human buccal epithelial cells by 80% in a dose-related fashion. In contrast, FBP54 had little effect on adhesion of group A streptococci to HEp-2 tissue culture cells. The fibronectin-binding domain of FBP54 has been localized to the first 89 N-terminal residues of the protein. Experiments using affinity-purified antibodies to this region indicated that the N terminus of FBP54 is exposed on the surface of streptococci in a manner that can interact with immobilized receptors. Analysis of sera from patients with post-streptococcal glomerulonephritis and acute rheumatic fever indicated that FBP54 is expressed in vivo and is immunogenic in the human host. These data indicate that FBP54 is a streptococcal adhesin that is expressed in the human host and that preferentially mediates adhesion to certain types of human cells.

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Selected References

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  • Abraham SN, Beachey EH, Simpson WA. Adherence of streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa to fibronectin-coated and uncoated epithelial cells. Infect Immun. 1983 Sep;41(3):1261–1268. [PMC free article] [PubMed]
  • Beachey EH, Courtney HS. Bacterial adherence: the attachment of group A streptococci to mucosal surfaces. Rev Infect Dis. 1987 Sep-Oct;9 (Suppl 5):S475–S481. [PubMed]
  • Beachey EH, Ofek I. Epithelial cell binding of group A streptococci by lipoteichoic acid on fimbriae denuded of M protein. J Exp Med. 1976 Apr 1;143(4):759–771. [PMC free article] [PubMed]
  • Bermudez LE, Shelton K, Young LS. Comparison of the ability of Mycobacterium avium, M. smegmatis and M. tuberculosis to invade and replicate within HEp-2 epithelial cells. Tuber Lung Dis. 1995 Jun;76(3):240–247. [PubMed]
  • Bronze MS, Courtney HS, Dale JB. Epitopes of group A streptococcal M protein that evoke cross-protective local immune responses. J Immunol. 1992 Feb 1;148(3):888–893. [PubMed]
  • Courtney HS, Bronze MS, Dale JB, Hasty DL. Analysis of the role of M24 protein in group A streptococcal adhesion and colonization by use of omega-interposon mutagenesis. Infect Immun. 1994 Nov;62(11):4868–4873. [PMC free article] [PubMed]
  • Courtney HS, Hasty DL, Dale JB, Poirier TP. A 28-kilodalton fibronectin-binding protein of group A streptococci. Curr Microbiol. 1992 Nov;25(5):245–250. [PubMed]
  • Courtney HS, Li Y, Dale JB, Hasty DL. Cloning, sequencing, and expression of a fibronectin/fibrinogen-binding protein from group A streptococci. Infect Immun. 1994 Sep;62(9):3937–3946. [PMC free article] [PubMed]
  • Courtney HS, Ofek I, Simpson WA, Hasty DL, Beachey EH. Binding of Streptococcus pyogenes to soluble and insoluble fibronectin. Infect Immun. 1986 Sep;53(3):454–459. [PMC free article] [PubMed]
  • Courtney HS, Simpson WA, Beachey EH. Binding of streptococcal lipoteichoic acid to fatty acid-binding sites on human plasma fibronectin. J Bacteriol. 1983 Feb;153(2):763–770. [PMC free article] [PubMed]
  • Courtney HS, Stanislawski L, Ofek I, Simpson WA, Hasty DL, Beachey EH. Localization of a lipoteichoic acid binding site to a 24-kilodalton NH2-terminal fragment of fibronectin. Rev Infect Dis. 1988 Jul-Aug;10 (Suppl 2):S360–S362. [PubMed]
  • Courtney HS, von Hunolstein C, Dale JB, Bronze MS, Beachey EH, Hasty DL. Lipoteichoic acid and M protein: dual adhesins of group A streptococci. Microb Pathog. 1992 Mar;12(3):199–208. [PubMed]
  • Dale JB, Baird RW, Courtney HS, Hasty DL, Bronze MS. Passive protection of mice against group A streptococcal pharyngeal infection by lipoteichoic acid. J Infect Dis. 1994 Feb;169(2):319–323. [PubMed]
  • Dale JB, Chiang EY, Lederer JW. Recombinant tetravalent group A streptococcal M protein vaccine. J Immunol. 1993 Aug 15;151(4):2188–2194. [PubMed]
  • Fischetti VA, Hodges WM, Hruby DE. Protection against streptococcal pharyngeal colonization with a vaccinia: M protein recombinant. Science. 1989 Jun 23;244(4911):1487–1490. [PubMed]
  • Fischetti VA, Medaglini D, Oggioni M, Pozzi G. Expression of foreign proteins on gram-positive commensal bacteria for mucosal vaccine delivery. Curr Opin Biotechnol. 1993 Oct;4(5):603–610. [PubMed]
  • Grabovskaya KB, Totoljan AA, Rýc M, Havlícek J, Burova LA, Bícová R. Adherence of group A streptococci to epithelial cells in tissue culture. Zentralbl Bakteriol A. 1980 Aug;247(3):303–314. [PubMed]
  • Hanski E, Caparon M. Protein F, a fibronectin-binding protein, is an adhesin of the group A streptococcus Streptococcus pyogenes. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6172–6176. [PubMed]
  • Hasty DL, Ofek I, Courtney HS, Doyle RJ. Multiple adhesins of streptococci. Infect Immun. 1992 Jun;60(6):2147–2152. [PMC free article] [PubMed]
  • Kreikemeyer B, Talay SR, Chhatwal GS. Characterization of a novel fibronectin-binding surface protein in group A streptococci. Mol Microbiol. 1995 Jul;17(1):137–145. [PubMed]
  • McKee ML, Melton-Celsa AR, Moxley RA, Francis DH, O'Brien AD. Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells. Infect Immun. 1995 Sep;63(9):3739–3744. [PMC free article] [PubMed]
  • Natanson S, Sela S, Moses AE, Musser JM, Caparon MG, Hanski E. Distribution of fibronectin-binding proteins among group A streptococci of different M types. J Infect Dis. 1995 Apr;171(4):871–878. [PubMed]
  • Ofek I, Courtney HS, Schifferli DM, Beachey EH. Enzyme-linked immunosorbent assay for adherence of bacteria to animal cells. J Clin Microbiol. 1986 Oct;24(4):512–516. [PMC free article] [PubMed]
  • Okada N, Liszewski MK, Atkinson JP, Caparon M. Membrane cofactor protein (CD46) is a keratinocyte receptor for the M protein of the group A streptococcus. Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2489–2493. [PubMed]
  • Pancholi V, Fischetti VA. A major surface protein on group A streptococci is a glyceraldehyde-3-phosphate-dehydrogenase with multiple binding activity. J Exp Med. 1992 Aug 1;176(2):415–426. [PMC free article] [PubMed]
  • Perez-Casal J, Okada N, Caparon MG, Scott JR. Role of the conserved C-repeat region of the M protein of Streptococcus pyogenes. Mol Microbiol. 1995 Mar;15(5):907–916. [PubMed]
  • Rakonjac JV, Robbins JC, Fischetti VA. DNA sequence of the serum opacity factor of group A streptococci: identification of a fibronectin-binding repeat domain. Infect Immun. 1995 Feb;63(2):622–631. [PMC free article] [PubMed]
  • Roos K, Holm SE, Grahn E, Lind L. Alpha-streptococci as supplementary treatment of recurrent streptococcal tonsillitis: a randomized placebo-controlled study. Scand J Infect Dis. 1993;25(1):31–35. [PubMed]
  • Sela S, Aviv A, Tovi A, Burstein I, Caparon MG, Hanski E. Protein F: an adhesin of Streptococcus pyogenes binds fibronectin via two distinct domains. Mol Microbiol. 1993 Dec;10(5):1049–1055. [PubMed]
  • Simpson WA, Beachey EH. Adherence of group A streptococci to fibronectin on oral epithelial cells. Infect Immun. 1983 Jan;39(1):275–279. [PMC free article] [PubMed]
  • Simpson WA, Courtney HS, Ofek I. Interactions of fibronectin with streptococci: the role of fibronectin as a receptor for Streptococcus pyogenes. Rev Infect Dis. 1987 Jul-Aug;9 (Suppl 4):S351–S359. [PubMed]
  • Sokurenko EV, Courtney HS, Abraham SN, Klemm P, Hasty DL. Functional heterogeneity of type 1 fimbriae of Escherichia coli. Infect Immun. 1992 Nov;60(11):4709–4719. [PMC free article] [PubMed]
  • Stevens DL. Invasive group A streptococcal infections: the past, present and future. Pediatr Infect Dis J. 1994 Jun;13(6):561–566. [PubMed]
  • Summersgill JT, Sahney NN, Gaydos CA, Quinn TC, Ramirez JA. Inhibition of Chlamydia pneumoniae growth in HEp-2 cells pretreated with gamma interferon and tumor necrosis factor alpha. Infect Immun. 1995 Jul;63(7):2801–2803. [PMC free article] [PubMed]
  • Talay SR, Valentin-Weigand P, Jerlström PG, Timmis KN, Chhatwal GS. Fibronectin-binding protein of Streptococcus pyogenes: sequence of the binding domain involved in adherence of streptococci to epithelial cells. Infect Immun. 1992 Sep;60(9):3837–3844. [PMC free article] [PubMed]
  • VanHeyningen T, Fogg G, Yates D, Hanski E, Caparon M. Adherence and fibronectin binding are environmentally regulated in the group A streptococci. Mol Microbiol. 1993 Sep;9(6):1213–1222. [PubMed]
  • Visai L, Bozzini S, Raucci G, Toniolo A, Speziale P. Isolation and characterization of a novel collagen-binding protein from Streptococcus pyogenes strain 6414. J Biol Chem. 1995 Jan 6;270(1):347–353. [PubMed]
  • Wang JR, Stinson MW. M protein mediates streptococcal adhesion to HEp-2 cells. Infect Immun. 1994 Feb;62(2):442–448. [PMC free article] [PubMed]
  • Wang JR, Stinson MW. Streptococcal M6 protein binds to fucose-containing glycoproteins on cultured human epithelial cells. Infect Immun. 1994 Apr;62(4):1268–1274. [PMC free article] [PubMed]
  • Zardi L, Carnemolla B, Balza E, Borsi L, Castellani P, Rocco M, Siri A. Elution of fibronectin proteolytic fragments from a hydroxyapatite chromatography column. A simple procedure for the purification of fibronectin domains. Eur J Biochem. 1985 Feb 1;146(3):571–579. [PubMed]

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