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Gut. 1998 May; 42(5): 721–726.
PMCID: PMC1727121

Liver/kidney microsomal antibody type 1 and liver cytosol antibody type 1 concentrations in type 2 autoimmune hepatitis


Background—Liver/kidney microsomal antibody type 1 (LKM1) and liver cytosol antibody type 1 (LC1) are the serological markers of type 2 autoimmune hepatitis (AIH).
Aims—Since LKM1 and LC1 react against two distinct liver specific autoantigens (cytochrome P450IID6 (CYP2D6) and a 58 kDa cytosolic polypeptide respectively), the aim was to see whether LKM1 and LC1 concentrations correlate with liver disease activity.
Patients—Twenty one patients with type 2 AIH were studied.
Methods—All sera were tested by indirect immunofluorescence, counterimmunoelectrophoresis, and immunoblotting visualised by enhanced chemiluminescence. To evaluate LKM1 and LC1 levels, the 50 kDa microsomal reactivity (corresponding to CYP2D6) and the 58 kDa cytosolic reactivity were quantified by densitometric analysis.
Results—Seven patients were positive for LKM1, nine for LC1, and five for both. Serial serum samples at onset and during immunosuppressive treatment were analysed in 13 patients (four positive for LKM1, six positive for LC1 and three positive for both). During remission, LKM1 concentration remained essentially unchanged in six of seven patients, and decreased in only one. Conversely, in two of nine patients, LC1 was completely lost, and, in the remaining seven, LC1 concentration was reduced by more than 50%. After immunosuppression tapering or withdrawal, flare ups of liver necrosis ensued with increasing LC1 concentration, but not LKM1.
Conclusions—LC1 concentration, at variance with that of LKM1, parallels liver disease activity, and its participation in the pathogenic mechanisms of liver injury can be hypothesised.

Keywords: autoantibodies; immunoblotting; LKM1; LC1; immunosuppression

Figure 2
(A) Representative behaviour of liver cytosol antibody type 1 (LC1) concentration and serum alanine aminotransferase (ALT) levels during immunosuppression in patient 19. LC1 immunoreactivity against the 58 kDa (kD) human liver cytosolic (HLC) polypeptide ...
Figure 1
Liver cytosol antibody type 1 (LC1) (A) and liver/kidney microsomal antibody type 1 (LKM1) (B) concentrations were evaluated at onset and during treatment induced remission in patients with type 2 autoimmune hepatitis. LC1, but not LKM1, was decreased significantly ...
Figure 3
Liver cytosol antibody type 1 (LC1) (A; patient 12) and liver/kidney microsomal antibody type 1 (LKM1) (B; patient 9) concentrations were sequentially reported in parallel with serum alanine aminotransferase (ALT) levels. ALT flares occurred during immunosuppression ...

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