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Philos Trans R Soc Lond B Biol Sci. 1999 January 29; 354(1379): 65–76.
PMCID: PMC1692445

Documenting the diet in ancient human populations through stable isotope analysis of hair.

Abstract

Fundamental to the understanding of human history is the ability to make interpretations based on artefacts and other remains which are used to gather information about an ancient population. Sequestered in the organic matrices of these remains can be information, for example, concerning incidence of disease, genetic defects and diet. Stable isotopic compositions, especially those made on isolates of collagen from bones, have been used to help suggest principal dietary components. A significant problem in the use of collagen is its long-term stability, and the possibility of isotopic alteration during early diagenesis, or through contaminating condensation reactions. In this study, we suggest that a commonly overlooked material, human hair, may represent an ideal material to be used in addressing human diets of ancient civilizations. Through the analysis of the amino-acid composition of modern hair, as well as samples that were subjected to radiation (thus simulating ageing of the hair) and hair from humans that is up to 5200 years old, we have observed little in the way of chemical change. The principal amino acids observed in all of these samples are essentially identical in relative abundances and content. Dominating the compositions are serine, glutamic acid, threonine, glycine and leucine, respectively accounting for approximately 15%, 17%, 10%, 8% and 8% of the total hydrolysable amino acids. Even minor components (for example, alanine, valine, isoleucine) show similar constancy between the samples of different ages. This constancy clearly indicates minimal alteration of the amino-acid composition of the hair. Further, it would indicate that hair is well preserved and is amenable to isotopic analysis as a tool for distinguishing sources of nutrition. Based on this observation, we have isotopically characterized modern individuals for whom the diet has been documented. Both stable nitrogen and carbon isotope compositions were assessed, and together provide an indication of trophic status, and principal type (C3 or C4) of vegetation consumed. True vegans have nitrogen isotope compositions of about 7/1000 whereas humans consuming larger amounts of meat, eggs, or milk are more enriched in the heavy nitrogen isotope. We have also analysed large cross-sections of modern humans from North America and Europe to provide an indication of the variability seen in a population (the supermarket diet). There is a wide diversity in both carbon and nitrogen isotope values based at least partially on the levels of seafood, corn-fed beef and grains in the diets. Following analysis of the ancient hair, we have observed similar trends in certain ancient populations. For example, the Coptics of Egypt (1000 BP) and Chinchorro of Chile (5000-800 BP) have diets of similar diversity to those observed in the modern group but were isotopically influenced by local nutritional sources. In other ancient hair (Egyptian Late Middle Kingdom mummies, ca. 4000 BP), we have observed a much more uniform isotopic signature, indicating a more constant diet. We have also recognized a primary vegetarian component in the diet of the Neolithic Ice Man of the Oetztaler Alps (5200 BP). In certain cases, it appears that sulphur isotopes may help to further constrain dietary interpretations, owing to the good preservation and sulphur content of hair. It appears that analysis of the often-overlooked hair in archaeological sites may represent a significant new approach for understanding ancient human communities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Böckle B, Galunsky B, Müller R. Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530. Appl Environ Microbiol. 1995 Oct;61(10):3705–3710. [PMC free article] [PubMed]
  • Chisholm BS, Nelson DE, Schwarcz HP. Stable-carbon isotope ratios as a measure of marine versus terrestrial protein in ancient diets. Science. 1982 Jun 4;216(4550):1131–1132. [PubMed]
  • Friedrich AB, Antranikian G. Keratin Degradation by Fervidobacterium pennavorans, a Novel Thermophilic Anaerobic Species of the Order Thermotogales. Appl Environ Microbiol. 1996 Aug;62(8):2875–2882. [PMC free article] [PubMed]
  • Katzenberg MA, Saunders SR, Fitzgerald WR. Age differences in stable carbon and nitrogen isotope ratios in a population of prehistoric maize horticulturists. Am J Phys Anthropol. 1993 Mar;90(3):267–281. [PubMed]
  • Dybing E, Aune T, Nelson SD. Metabolic activation of 2,4-diaminoanisole, a hair-dye component--III. Role of cytochrome P-450 metabolism in irreversible binding in vivo. Biochem Pharmacol. 1979;28(1):51–55. [PubMed]
  • Lubec G, Weninger M, Anderson SR. Racemization and oxidation studies of hair protein in the Homo tirolensis. FASEB J. 1994 Nov;8(14):1166–1169. [PubMed]
  • Nakagawa A, Kitagawa A, Asami M, Nakamura K, Schoeller DA, Slater R, Minagawa M, Kaplan IR. Evaluation of isotope ratio (IR) mass spectrometry for the study of drug metabolism. Biomed Mass Spectrom. 1985 Sep;12(9):502–506. [PubMed]
  • Nakamura K, Schoeller DA, Winkler FJ, Schmidt HL. Geographical variations in the carbon isotope composition of the diet and hair in contemporary man. Biomed Mass Spectrom. 1982 Sep;9(9):390–394. [PubMed]
  • Schoeninger MJ, DeNiro MJ, Tauber H. Stable nitrogen isotope ratios of bone collagen reflect marine and terrestrial components of prehistoric human diet. Science. 1983 Jun 24;220(4604):1381–1383. [PubMed]
  • Tauber H. 13C evidence for dietary habits of prehistoric man in Denmark. Nature. 1981 Jul 23;292(5821):332–333. [PubMed]
  • White CD, Schwarcz HP. Temporal trends in stable isotopes for Nubian mummy tissues. Am J Phys Anthropol. 1994 Feb;93(2):165–187. [PubMed]
  • Yoshinaga J, Minagawa M, Suzuki T, Ohtsuka R, Kawabe T, Inaoka T, Akimichi T. Stable carbon and nitrogen isotopic composition of diet and hair of Gidra-speaking Papuans. Am J Phys Anthropol. 1996 May;100(1):23–34. [PubMed]

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