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Appl Environ Microbiol. 1996 May; 62(5): 1747–1751.
PMCID: PMC167949

A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus.


Unicellular cyanobacteria of the genus Synechococcus are among the most abundant members of the picoplankton in the open ocean, and their contribution to primary production is considerable. While several isolates have been used for physiological, biochemical, and molecular studies of their unique adaptations to the marine environment, it has become necessary to develop molecular genetic methods for one or more model open-ocean cyanobacteria in order for studies of these organisms and their unique properties to progress. A number of molecular tools for the genetic manipulation of Synechococcus sp. strains WH7803, WH8102, and WH8103 have been developed. These include a plating technique for obtaining isolated colonies at high efficiencies and a conjugation method for introducing both a replicative vector and a suicide vector. In addition, a method for the generation of random, tagged chromosomal insertions (N. Dolganov and A. R. Grossman, J. Bacteriol. 175:7644-7651, 1993; N. F. Tsinoremas, A. K. Kutach, C. A. Strayer, and S. S. Golden, J. Bacteriol. 176:6764-6768, 1994) has been applied to these organisms.

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Selected References

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  • Alberte RS, Wood AM, Kursar TA, Guillard RR. Novel Phycoerythrins in Marine Synechococcus spp. : Characterization and Evolutionary and Ecological Implications. Plant Physiol. 1984 Jul;75(3):732–739. [PubMed]
  • Bagdasarian M, Lurz R, Rückert B, Franklin FC, Bagdasarian MM, Frey J, Timmis KN. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene. 1981 Dec;16(1-3):237–247. [PubMed]
  • Casadaban MJ, Cohen SN. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. [PubMed]
  • Dolganov N, Grossman AR. Insertional inactivation of genes to isolate mutants of Synechococcus sp. strain PCC 7942: isolation of filamentous strains. J Bacteriol. 1993 Dec;175(23):7644–7651. [PMC free article] [PubMed]
  • Elhai J, Wolk CP. Conjugal transfer of DNA to cyanobacteria. Methods Enzymol. 1988;167:747–754. [PubMed]
  • Golden SS, Brusslan J, Haselkorn R. Genetic engineering of the cyanobacterial chromosome. Methods Enzymol. 1987;153:215–231. [PubMed]
  • Gormley EP, Davies J. Transfer of plasmid RSF1010 by conjugation from Escherichia coli to Streptomyces lividans and Mycobacterium smegmatis. J Bacteriol. 1991 Nov;173(21):6705–6708. [PMC free article] [PubMed]
  • Meyer R, Figurski D, Helinski DR. Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Mol Gen Genet. 1977 Apr 29;152(3):129–135. [PubMed]
  • Ong LJ, Glazer AN. R-phycocyanin II, a new phycocyanin occurring in marine Synechococcus species. Identification of the terminal energy acceptor bilin in phycocyanins. J Biol Chem. 1987 May 5;262(13):6323–6327. [PubMed]
  • Ong LJ, Glazer AN, Waterbury JB. An unusual phycoerythrin from a marine cyanobacterium. Science. 1984 Apr 6;224(4644):80–83. [PubMed]
  • Porter RD. DNA transformation. Methods Enzymol. 1988;167:703–712. [PubMed]
  • Scanlan DJ, Mann NH, Carr NG. The response of the picoplanktonic marine cyanobacterium Synechococcus species WH7803 to phosphate starvation involves a protein homologous to the periplasmic phosphate-binding protein of Escherichia coli. Mol Microbiol. 1993 Oct;10(1):181–191. [PubMed]
  • Thiel T, Poo H. Transformation of a filamentous cyanobacterium by electroporation. J Bacteriol. 1989 Oct;171(10):5743–5746. [PMC free article] [PubMed]
  • Tsinoremas NF, Kutach AK, Strayer CA, Golden SS. Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination. J Bacteriol. 1994 Nov;176(21):6764–6768. [PMC free article] [PubMed]
  • Waterbury JB, Valois FW. Resistance to co-occurring phages enables marine synechococcus communities to coexist with cyanophages abundant in seawater. Appl Environ Microbiol. 1993 Oct;59(10):3393–3399. [PMC free article] [PubMed]
  • Waterbury JB, Willey JM, Franks DG, Valois FW, Watson SW. A cyanobacterium capable of swimming motility. Science. 1985 Oct 4;230(4721):74–76. [PubMed]
  • Wilbanks SM, Glazer AN. Rod structure of a phycoerythrin II-containing phycobilisome. I. Organization and sequence of the gene cluster encoding the major phycobiliprotein rod components in the genome of marine Synechococcus sp. WH8020. J Biol Chem. 1993 Jan 15;268(2):1226–1235. [PubMed]
  • Wilbanks SM, Glazer AN. Rod structure of a phycoerythrin II-containing phycobilisome. II. Complete sequence and bilin attachment site of a phycoerythrin gamma subunit. J Biol Chem. 1993 Jan 15;268(2):1236–1241. [PubMed]
  • Willey JM, Waterbury JB. Chemotaxis toward Nitrogenous Compounds by Swimming Strains of Marine Synechococcus spp. Appl Environ Microbiol. 1989 Aug;55(8):1888–1894. [PMC free article] [PubMed]
  • Willey JM, Waterbury JB, Greenberg EP. Sodium-coupled motility in a swimming cyanobacterium. J Bacteriol. 1987 Aug;169(8):3429–3434. [PMC free article] [PubMed]
  • Wilson WH, Joint IR, Carr NG, Mann NH. Isolation and Molecular Characterization of Five Marine Cyanophages Propagated on Synechococcus sp. Strain WH7803. Appl Environ Microbiol. 1993 Nov;59(11):3736–3743. [PMC free article] [PubMed]

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