PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
 
Appl Environ Microbiol. 1996 March; 62(3): 892–897.
PMCID: PMC167854

Isolation, partial characterization, and mode of action of Acidocin J1132, a two-component bacteriocin produced by Lactobacillus acidophilus JCM 1132.

Abstract

Lactobacillus acidophilus JCM 1132 produces a heat-stable, two-component bacteriocin designated acidocin J1132 that has a narrow inhibitory spectrum. Maximum production of acidocin J1132 in MRS broth was detected at pH 5.0. Acidocin J1132 was purified by ammonium sulfate precipitation and sequential cation exchange and reversed-phase chromatographies. Acidocin J1132 activity was associated with two components, termed alpha and beta. On the basis of N-terminal amino acid sequencing and the molecular masses of the alpha and beta components, it is interpreted that the compounds differ by an additional glycine residue in the beta component. Both alpha and beta had inhibitory activity, and an increase in activity by the complementary action of the two components was observed. Acidocin J1132 is bactericidal and dissipates the membrane potential and the pH gradient in sensitive cells, which affect such proton motive force-dependent processes as amino acid transport. Acidocin J1132 also caused efflux of preaccumulated amino acid taken up via a unidirectional ATP-driven transport system. Secondary structure prediction revealed the presence of an amphiphilic alpha-helix region that could form hydrophilic pores. These results suggest that acidocin J1132 is a pore-forming bacteriocin that creates cell membrane channels through the "barrel-stave" mechanism.

Full Text

The Full Text of this article is available as a PDF (249K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abee T, Klaenhammer TR, Letellier L. Kinetic studies of the action of lactacin F, a bacteriocin produced by Lactobacillus johnsonii that forms poration complexes in the cytoplasmic membrane. Appl Environ Microbiol. 1994 Mar;60(3):1006–1013. [PMC free article] [PubMed]
  • Allison GE, Fremaux C, Klaenhammer TR. Expansion of bacteriocin activity and host range upon complementation of two peptides encoded within the lactacin F operon. J Bacteriol. 1994 Apr;176(8):2235–2241. [PMC free article] [PubMed]
  • Barefoot SF, Klaenhammer TR. Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol. 1983 Jun;45(6):1808–1815. [PMC free article] [PubMed]
  • Barefoot SF, Klaenhammer TR. Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B. Antimicrob Agents Chemother. 1984 Sep;26(3):328–334. [PMC free article] [PubMed]
  • Bruno ME, Montville TJ. Common mechanistic action of bacteriocins from lactic Acid bacteria. Appl Environ Microbiol. 1993 Sep;59(9):3003–3010. [PMC free article] [PubMed]
  • Chikindas ML, García-Garcerá MJ, Driessen AJ, Ledeboer AM, Nissen-Meyer J, Nes IF, Abee T, Konings WN, Venema G. Pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC1.0, forms hydrophilic pores in the cytoplasmic membrane of target cells. Appl Environ Microbiol. 1993 Nov;59(11):3577–3584. [PMC free article] [PubMed]
  • Daba H, Lacroix C, Huang J, Simard RE, Lemieux L. Simple method of purification and sequencing of a bacteriocin produced by Pediococcus acidilactici UL5. J Appl Bacteriol. 1994 Dec;77(6):682–688. [PubMed]
  • González B, Arca P, Mayo B, Suárez JE. Detection, purification, and partial characterization of plantaricin C, a bacteriocin produced by a Lactobacillus plantarum strain of dairy origin. Appl Environ Microbiol. 1994 Jun;60(6):2158–2163. [PMC free article] [PubMed]
  • Hastings JW, Sailer M, Johnson K, Roy KL, Vederas JC, Stiles ME. Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J Bacteriol. 1991 Dec;173(23):7491–7500. [PMC free article] [PubMed]
  • Jiménez-Díaz R, Rios-Sánchez RM, Desmazeaud M, Ruiz-Barba JL, Piard JC. Plantaricins S and T, Two New Bacteriocins Produced by Lactobacillus plantarum LPCO10 Isolated from a Green Olive Fermentation. Appl Environ Microbiol. 1993 May;59(5):1416–1424. [PMC free article] [PubMed]
  • Joerger MC, Klaenhammer TR. Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J Bacteriol. 1986 Aug;167(2):439–446. [PMC free article] [PubMed]
  • Kanatani K, Oshimura M, Sano K. Isolation and characterization of acidocin A and cloning of the bacteriocin gene from Lactobacillus acidophilus. Appl Environ Microbiol. 1995 Mar;61(3):1061–1067. [PMC free article] [PubMed]
  • Kashket ER, Blanchard AG, Metzger WC. Proton motive force during growth of Streptococcus lactis cells. J Bacteriol. 1980 Jul;143(1):128–134. [PMC free article] [PubMed]
  • Klaenhammer TR. Bacteriocins of lactic acid bacteria. Biochimie. 1988 Mar;70(3):337–349. [PubMed]
  • Klaenhammer TR. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev. 1993 Sep;12(1-3):39–85. [PubMed]
  • Konings WN, Poolman B, Driessen AJ. Bioenergetics and solute transport in lactococci. Crit Rev Microbiol. 1989;16(6):419–476. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Lear JD, Wasserman ZR, DeGrado WF. Synthetic amphiphilic peptide models for protein ion channels. Science. 1988 May 27;240(4856):1177–1181. [PubMed]
  • Muriana PM, Klaenhammer TR. Conjugal Transfer of Plasmid-Encoded Determinants for Bacteriocin Production and Immunity in Lactobacillus acidophilus 88. Appl Environ Microbiol. 1987 Mar;53(3):553–560. [PMC free article] [PubMed]
  • Muriana PM, Klaenhammer TR. Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl Environ Microbiol. 1991 Jan;57(1):114–121. [PMC free article] [PubMed]
  • Nielsen JW, Dickson JS, Crouse JD. Use of a bacteriocin produced by Pediococcus acidilactici to inhibit Listeria monocytogenes associated with fresh meat. Appl Environ Microbiol. 1990 Jul;56(7):2142–2145. [PMC free article] [PubMed]
  • Nissen-Meyer J, Holo H, Håvarstein LS, Sletten K, Nes IF. A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides. J Bacteriol. 1992 Sep;174(17):5686–5692. [PMC free article] [PubMed]
  • Nissen-Meyer J, Larsen AG, Sletten K, Daeschel M, Nes IF. Purification and characterization of plantaricin A, a Lactobacillus plantarum bacteriocin whose activity depends on the action of two peptides. J Gen Microbiol. 1993 Sep;139(9):1973–1978. [PubMed]
  • Ojcius DM, Young JD. Cytolytic pore-forming proteins and peptides: is there a common structural motif? Trends Biochem Sci. 1991 Jun;16(6):225–229. [PubMed]
  • Poolman B, Hellingwerf KJ, Konings WN. Regulation of the glutamate-glutamine transport system by intracellular pH in Streptococcus lactis. J Bacteriol. 1987 May;169(5):2272–2276. [PMC free article] [PubMed]
  • Poolman B, Smid EJ, Konings WN. Kinetic properties of a phosphate-bond-driven glutamate-glutamine transport system in Streptococcus lactis and Streptococcus cremoris. J Bacteriol. 1987 Jun;169(6):2755–2761. [PMC free article] [PubMed]
  • Quadri LE, Sailer M, Roy KL, Vederas JC, Stiles ME. Chemical and genetic characterization of bacteriocins produced by Carnobacterium piscicola LV17B. J Biol Chem. 1994 Apr 22;269(16):12204–12211. [PubMed]
  • Rottenberg H. The measurement of membrane potential and deltapH in cells, organelles, and vesicles. Methods Enzymol. 1979;55:547–569. [PubMed]
  • Ruhr E, Sahl HG. Mode of action of the peptide antibiotic nisin and influence on the membrane potential of whole cells and on cytoplasmic and artificial membrane vesicles. Antimicrob Agents Chemother. 1985 May;27(5):841–845. [PMC free article] [PubMed]
  • Ruiz-Barba JL, Cathcart DP, Warner PJ, Jiménez-Díaz R. Use of Lactobacillus plantarum LPCO10, a Bacteriocin Producer, as a Starter Culture in Spanish-Style Green Olive Fermentations. Appl Environ Microbiol. 1994 Jun;60(6):2059–2064. [PMC free article] [PubMed]
  • Schillinger U, Kaya M, Lücke FK. Behaviour of Listeria monocytogenes in meat and its control by a bacteriocin-producing strain of Lactobacillus sake. J Appl Bacteriol. 1991 Jun;70(6):473–478. [PubMed]
  • Schnell N, Entian KD, Schneider U, Götz F, Zähner H, Kellner R, Jung G. Prepeptide sequence of epidermin, a ribosomally synthesized antibiotic with four sulphide-rings. Nature. 1988 May 19;333(6170):276–278. [PubMed]
  • Tagg JR, McGiven AR. Assay system for bacteriocins. Appl Microbiol. 1971 May;21(5):943–943. [PMC free article] [PubMed]
  • van Belkum MJ, Hayema BJ, Geis A, Kok J, Venema G. Cloning of two bacteriocin genes from a lactococcal bacteriocin plasmid. Appl Environ Microbiol. 1989 May;55(5):1187–1191. [PMC free article] [PubMed]
  • van Belkum MJ, Hayema BJ, Jeeninga RE, Kok J, Venema G. Organization and nucleotide sequences of two lactococcal bacteriocin operons. Appl Environ Microbiol. 1991 Feb;57(2):492–498. [PMC free article] [PubMed]
  • van Belkum MJ, Kok J, Venema G, Holo H, Nes IF, Konings WN, Abee T. The bacteriocin lactococcin A specifically increases permeability of lactococcal cytoplasmic membranes in a voltage-independent, protein-mediated manner. J Bacteriol. 1991 Dec;173(24):7934–7941. [PMC free article] [PubMed]
  • Venema K, Abee T, Haandrikman AJ, Leenhouts KJ, Kok J, Konings WN, Venema G. Mode of Action of Lactococcin B, a Thiol-Activated Bacteriocin from Lactococcus lactis. Appl Environ Microbiol. 1993 Apr;59(4):1041–1048. [PMC free article] [PubMed]
  • Winkowski K, Crandall AD, Montville TJ. Inhibition of Listeria monocytogenes by Lactobacillus bavaricus MN in beef systems at refrigeration temperatures. Appl Environ Microbiol. 1993 Aug;59(8):2552–2557. [PMC free article] [PubMed]
  • Worobo RW, Henkel T, Sailer M, Roy KL, Vederas JC, Stiles ME. Characteristics and genetic determinant of a hydrophobic peptide bacteriocin, carnobacteriocin A, produced by Carnobacterium piscicola LV17A. Microbiology. 1994 Mar;140(Pt 3):517–526. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)