Search tips
Search criteria 


Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. 1996 January; 62(1): 162–167.
PMCID: PMC167783

Phylogenetic position of yeastlike endosymbionts of anobiid beetles.


The Anobiid beetles Stegobium paniceum and Lasioderma serricorne possess the intracellular yeastlike symbionts Symbiotaphrina buchneri and Symbiotaphrina kochii, respectively, in the mycetome between the foregut and midgut. The nucleotide sequences of the small-subunit rRNA-encoding genes of the symbionts were determined for phylogenetic analysis. Five group I introns were found in the small-subunit rRNA-encoding genes of S. buchneri, but S. kochii lacked introns. The phylogenetic position of both symbionts was close to the class Discomycetes, which is a paraphyletic group. The two symbionts formed a monophyletic group distinct from the other major lineages. Both appear to have parted from other filamentous fungi during the early radiation of the euascomycetes and to have later become obligatory partners of the beetles.

Full Text

The Full Text of this article is available as a PDF (237K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Baumann P, Lai C, Baumann L, Rouhbakhsh D, Moran NA, Clark MA. Mutualistic associations of aphids and prokaryotes: biology of the genus buchnera. Appl Environ Microbiol. 1995 Jan;61(1):1–7. [PMC free article] [PubMed]
  • Bej AK, Mahbubani MH, Atlas RM. Amplification of nucleic acids by polymerase chain reaction (PCR) and other methods and their applications. Crit Rev Biochem Mol Biol. 1991;26(3-4):301–334. [PubMed]
  • Belfort M. Self-splicing introns in prokaryotes: migrant fossils? Cell. 1991 Jan 11;64(1):9–11. [PubMed]
  • Belfort M. An expanding universe of introns. Science. 1993 Nov 12;262(5136):1009–1010. [PubMed]
  • Bhattacharya D, Surek B, Rüsing M, Damberger S, Melkonian M. Group I introns are inherited through common ancestry in the nuclear-encoded rRNA of Zygnematales (Charophyceae). Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):9916–9920. [PubMed]
  • Bismanis JE. Endosymbionts of Sitodrepa panicea. Can J Microbiol. 1976 Oct;22(10):1415–1424. [PubMed]
  • Burke JM, Belfort M, Cech TR, Davies RW, Schweyen RJ, Shub DA, Szostak JW, Tabak HF. Structural conventions for group I introns. Nucleic Acids Res. 1987 Sep 25;15(18):7217–7221. [PMC free article] [PubMed]
  • Cech TR. The generality of self-splicing RNA: relationship to nuclear mRNA splicing. Cell. 1986 Jan 31;44(2):207–210. [PubMed]
  • Damberger SH, Gutell RR. A comparative database of group I intron structures. Nucleic Acids Res. 1994 Sep;22(17):3508–3510. [PMC free article] [PubMed]
  • Davies RW, Waring RB, Ray JA, Brown TA, Scazzocchio C. Making ends meet: a model for RNA splicing in fungal mitochondria. Nature. 1982 Dec 23;300(5894):719–724. [PubMed]
  • De Jonckheere JF. Evidence for the ancestral origin of group I introns in the SSUrDNA of Naegleria spp. J Eukaryot Microbiol. 1994 Sep-Oct;41(5):457–463. [PubMed]
  • DePriest PT, Been MD. Numerous group I introns with variable distributions in the ribosomal DNA of a lichen fungus. J Mol Biol. 1992 Nov 20;228(2):315–321. [PubMed]
  • De Wachter R, Neefs JM, Goris A, Van de Peer Y. The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron. Nucleic Acids Res. 1992 Mar 25;20(6):1251–1257. [PMC free article] [PubMed]
  • Dujon B. Group I introns as mobile genetic elements: facts and mechanistic speculations--a review. Gene. 1989 Oct 15;82(1):91–114. [PubMed]
  • Dujon B, Belfort M, Butow RA, Jacq C, Lemieux C, Perlman PS, Vogt VM. Mobile introns: definition of terms and recommended nomenclature. Gene. 1989 Oct 15;82(1):115–118. [PubMed]
  • Gargas A, DePriest PT, Taylor JW. Positions of multiple insertions in SSU rDNA of lichen-forming fungi. Mol Biol Evol. 1995 Mar;12(2):208–218. [PubMed]
  • Gargas A, Taylor JW. Phylogeny of Discomycetes and early radiations of the apothecial Ascomycotina inferred from SSU rDNA sequence data. Exp Mycol. 1995 Mar;19(1):7–15. [PubMed]
  • Kuhsel MG, Strickland R, Palmer JD. An ancient group I intron shared by eubacteria and chloroplasts. Science. 1990 Dec 14;250(4987):1570–1573. [PubMed]
  • Mankin AS, Skryabin KG, Rubtsov PM. Identification of ten additional nucleotides in the primary structure of yeast 18S rRNA. Gene. 1986;44(1):143–145. [PubMed]
  • Neefs JM, Van de Peer Y, De Rijk P, Chapelle S, De Wachter R. Compilation of small ribosomal subunit RNA structures. Nucleic Acids Res. 1993 Jul 1;21(13):3025–3049. [PMC free article] [PubMed]
  • Nishida H, Blanz PA, Sugiyama J. The higher fungus Protomyces inouyei has two group I introns in the 18S rRNA gene. J Mol Evol. 1993 Jul;37(1):25–28. [PubMed]
  • Noda H, Kawahara N. Electrophoretic karyotype of intracellular yeast-like symbiotes in rice planthoppers and anobiid beetles. J Invertebr Pathol. 1995 Mar;65(2):118–124. [PubMed]
  • Noda H, Nakashima N, Koizumi M. Phylogenetic position of yeast-like symbiotes of rice planthoppers based on partial 18S rDNA sequences. Insect Biochem Mol Biol. 1995 May;25(5):639–646. [PubMed]
  • Pearson WR, Lipman DJ. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. [PubMed]
  • Rogers SO, Yan ZH, Shinohara M, LoBuglio KF, Wang CJ. Messenger RNA intron in the nuclear 18s ribosomal RNA gene of deuteromycetes. Curr Genet. 1993;23(4):338–342. [PubMed]
  • Sogin ML, Edman JC. A self-splicing intron in the small subunit rRNA gene of Pneumocystis carinii. Nucleic Acids Res. 1989 Jul 11;17(13):5349–5359. [PMC free article] [PubMed]
  • Unterman BM, Baumann P, McLean DL. Pea aphid symbiont relationships established by analysis of 16S rRNAs. J Bacteriol. 1989 Jun;171(6):2970–2974. [PMC free article] [PubMed]
  • van der WALT J. The mycetome symbiont of Lasioderma serricorne. Antonie Van Leeuwenhoek. 1961;27:362–366. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)