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Appl Environ Microbiol. 1996 January; 62(1): 94–99.
PMCID: PMC167777

Degradation of cocaine by a mixed culture of Pseudomonas fluorescens MBER and Comamonas acidovorans MBLF.


A mixed culture that could utilize cocaine as the sole source of carbon and energy for growth was isolated by selective enrichment. The individual microorganisms within this mixed culture were identified as Pseudomonas fluorescens (termed MBER) and Comamonas acidovorans (termed MBLF). Each microorganism was shown to be unable to grow to any appreciable extent on 10 mM cocaine in the absence of the other. C. acidovorans MBLF was found to possess an inducible cocaine esterase which catalyzed the hydrolysis of cocaine to ecgonine methyl ester and benzoate. C. acidovorans was capable of growth on benzoate at concentrations below 5 mM but was unable to metabolize ecgonine methyl ester. P. fluorescens MBER was capable of growth on either benzoate as the sole source of carbon or ecgonine methyl ester as the sole source of carbon and nitrogen. P. fluorescens MBER was found to initiate the degradation of ecgonine methyl ester via ecgonine, pseudoecgonine, and pseudoecgonyl-coenzyme A. Subcellular studies resulted in the identification of an ecgonine methyl esterase, an ecgonine epimerase, and a pseudoecgonyl-coenzyme A synthetase which were induced by growth on ecgonine methyl ester or ecgonine. Further metabolism of the ecgonine moiety is postulated to involve nitrogen debridging, with the production of carbonyl-containing intermediates.

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Selected References

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  • Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. [PubMed]
  • Bartholomew BA, Smith MJ, Long MT, Darcy PJ, Trudgill PW, Hopper DJ. The isolation and identification of 6-hydroxycyclohepta-1,4-dione as a novel intermediate in the bacterial degradation of atropine. Biochem J. 1993 Jul 1;293(Pt 1):115–118. [PubMed]
  • Bartholomew BA, Smith MJ, Long MT, Darcy PJ, Trudgill PW, Hopper DJ. Tropine dehydrogenase: purification, some properties and an evaluation of its role in the bacterial metabolism of tropine. Biochem J. 1995 Apr 15;307(Pt 2):603–608. [PubMed]
  • Blakley ER. The microbial degradation of cyclohexanecarboxylic acid by a beta-oxidation pathway with simultaneous induction to the utilization of benzoate. Can J Microbiol. 1978 Jul;24(7):847–855. [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Britt AJ, Bruce NC, Lowe CR. Identification of a cocaine esterase in a strain of Pseudomonas maltophilia. J Bacteriol. 1992 Apr;174(7):2087–2094. [PMC free article] [PubMed]
  • Misra AL, Pontani RB, Mulé SJ. Separation of cocaine, some of its metabolites and congeners on glass fibre sheets. J Chromatogr. 1973 Jun 27;81(1):167–169. [PubMed]
  • Nathanson JA, Hunnicutt EJ, Kantham L, Scavone C. Cocaine as a naturally occurring insecticide. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9645–9648. [PubMed]
  • Overath P, Pauli G, Schairer HU. Fatty acid degradation in Escherichia coli. An inducible acyl-CoA synthetase, the mapping of old-mutations, and the isolation of regulatory mutants. Eur J Biochem. 1969 Feb;7(4):559–574. [PubMed]

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