PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
 
Appl Environ Microbiol. 1996 January; 62(1): 67–73.
PMCID: PMC167774

Description of a new polymer-secreting bacterium from a deep-sea hydrothermal vent, Alteromonas macleodii subsp. fijiensis, and preliminary characterization of the polymer.

Abstract

A deep-sea, aerobic, mesophilic, heterotrophic bacterium was isolated from fluid collected near an active hydrothermal vent. On the basis of phenotypic and phylogenetic analyses and DNA-DNA relatedness, strain ST716 could be assigned to the species Alteromonas macleodii as a new subspecies. This bacterium secreted an unusual high-molecular-weight polysaccharide in the presence of glucose in batch cultures. The viscosity of this exopolysaccharide is of the same order of magnitude as that of xanthan, another bacterial polysaccharide of industrial interest. This polysaccharide, produced during the stationary phase, contained glucose, mannose, pyruvated mannose, and galactose along with galacturonic acid and glucuronic acid.

Full Text

The Full Text of this article is available as a PDF (218K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Blumenkrantz N, Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. [PubMed]
  • Boyle CD, Reade AE. Characterization of two extracellular polysaccharides from marine bacteria. Appl Environ Microbiol. 1983 Aug;46(2):392–399. [PMC free article] [PubMed]
  • Christensen BE, Kjosbakken J, Smidsrød O. Partial Chemical and Physical Characterization of Two Extracellular Polysaccharides Produced by Marine, Periphytic Pseudomonas sp. Strain NCMB 2021. Appl Environ Microbiol. 1985 Oct;50(4):837–845. [PMC free article] [PubMed]
  • Elson LA, Morgan WT. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. [PubMed]
  • Fox GE, Wisotzkey JD, Jurtshuk P., Jr How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int J Syst Bacteriol. 1992 Jan;42(1):166–170. [PubMed]
  • Gauthier G, Gauthier M, Christen R. Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol. 1995 Oct;45(4):755–761. [PubMed]
  • Kamerling JP, Gerwig GJ, Vliegenthart JF, Clamp JR. Characterization by gas-liquid chromatography-mass spectrometry and proton-magnetic-resonance spectroscopy of pertrimethylsilyl methyl glycosides obtained in the methanolysis of glycoproteins and glycopeptides. Biochem J. 1975 Dec;151(3):491–495. [PubMed]
  • KING EO, WARD MK, RANEY DE. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed]
  • Lijour Y, Gentric E, Deslandes E, Guezennec J. Estimation of the sulfate content of hydrothermal vent bacterial polysaccharides by Fourier transform infrared spectroscopy. Anal Biochem. 1994 Aug 1;220(2):244–248. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Mittelman MW, Geesey GG. Copper-binding characteristics of exopolymers from a freshwater-sediment bacterium. Appl Environ Microbiol. 1985 Apr;49(4):846–851. [PMC free article] [PubMed]
  • Nazarenko EL, Zubkov VA, Shashkov AS, Knirel' Iu A, Gorshkova RP, Ivanova EP, Ovodov Iu S. Struktura povtoriaiushchegosia zvena kislogo polisakharida Alteromonas macleodii 2MM6. Bioorg Khim. 1993 Jul;19(7):740–751. [PubMed]
  • Rimington C. The carbohydrate complex of the serum proteins: Improved method for isolation and re-determination of structure. Isolation of glucosaminodimannose from proteins of ox blood. Biochem J. 1931;25(4):1062–1071. [PubMed]
  • Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. [PubMed]
  • Selvendran RR, March JF, Ring SG. Determination of aldoses and uronic acid content of vegetable fiber. Anal Biochem. 1979 Jul 15;96(2):282–292. [PubMed]
  • Sneath PH. Evidence from Aeromonas for genetic crossing-over in ribosomal sequences. Int J Syst Bacteriol. 1993 Jul;43(3):626–629. [PubMed]
  • Sritharan V, Barker RH., Jr A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR. Mol Cell Probes. 1991 Oct;5(5):385–395. [PubMed]
  • Umezawa H, Okami Y, Kurasawa S, Ohnuki T, Ishizuka M, Takeuchi T, Shiio T, Yugari Y. Marinactan, antitumor polysaccharide produced by marine bacteria. J Antibiot (Tokyo) 1983 May;36(5):471–477. [PubMed]
  • Vincent P, Pignet P, Talmont F, Bozzi L, Fournet B, Guezennec J, Jeanthon C, Prieur D. Production and Characterization of an Exopolysaccharide Excreted by a Deep-Sea Hydrothermal Vent Bacterium Isolated from the Polychaete Annelid Alvinella pompejana. Appl Environ Microbiol. 1994 Nov;60(11):4134–4141. [PMC free article] [PubMed]
  • Wrangstadh M, Conway PL, Kjelleberg S. The production and release of an extracellular polysaccharide during starvation of a marine Pseudomonas sp. and the effect thereof on adhesion. Arch Microbiol. 1986 Aug;145(3):220–227. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)