Search tips
Search criteria 


Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. 1995 May; 61(5): 2020–2022.
PMCID: PMC167468

Emergence of nylon oligomer degradation enzymes in Pseudomonas aeruginosa PAO through experimental evolution.


Through selective cultivation with 6-aminohexanoate linear dimer, a by-product of nylon-6 manufacture, as the sole source of carbon and nitrogen, Pseudomonas aeruginosa PAO, which initially has no enzyme activity to degrade this xenobiotic compound, was successfully expanded in its metabolic ability. Two new enzyme activities, 6-aminohexanoate cyclic dimer hydrolase and 6-aminohexanoate dimer hydrolase, were detected in the adapted strains.

Full Text

The Full Text of this article is available as a PDF (185K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Foster PL, Trimarchi JM. Adaptive reversion of a frameshift mutation in Escherichia coli by simple base deletions in homopolymeric runs. Science. 1994 Jul 15;265(5170):407–409. [PMC free article] [PubMed]
  • Hall BG, Clarke ND. Regulation of newly evolved enzymes. III Evolution of the ebg repressor during selection for enhanced lactase activity. Genetics. 1977 Feb;85(2):193–201. [PubMed]
  • Harris RS, Longerich S, Rosenberg SM. Recombination in adaptive mutation. Science. 1994 Apr 8;264(5156):258–260. [PubMed]
  • Holloway BW, Morgan AF. Genome organization in Pseudomonas. Annu Rev Microbiol. 1986;40:79–105. [PubMed]
  • Kanagawa K, Oishi M, Negoro S, Urabe I, Okada H. Characterization of the 6-aminohexanoate-dimer hydrolase from Pseudomonas sp. NK87. J Gen Microbiol. 1993 Apr;139(4):787–795. [PubMed]
  • Kinoshita S, Negoro S, Muramatsu M, Bisaria VS, Sawada S, Okada H. 6-Aminohexanoic acid cyclic dimer hydrolase. A new cyclic amide hydrolase produced by Achromobacter guttatus KI74. Eur J Biochem. 1977 Nov 1;80(2):489–495. [PubMed]
  • Kinoshita S, Terada T, Taniguchi T, Takene Y, Masuda S, Matsunaga N, Okada H. Purification and characterization of 6-aminohexanoic-acid-oligomer hydrolase of Flavobacterium sp. Ki72. Eur J Biochem. 1981 Jun 1;116(3):547–551. [PubMed]
  • Lenski RE, Mittler JE. The directed mutation controversy and neo-Darwinism. Science. 1993 Jan 8;259(5092):188–194. [PubMed]
  • Negoro S, Kakudo S, Urabe I, Okada H. A new nylon oligomer degradation gene (nylC) on plasmid pOAD2 from a Flavobacterium sp. J Bacteriol. 1992 Dec;174(24):7948–7953. [PMC free article] [PubMed]
  • Negoro S, Shinagawa H, Nakata A, Kinoshita S, Hatozaki T, Okada H. Plasmid control of 6-aminohexanoic acid cyclic dimer degradation enzymes of Flavobacterium sp. KI72. J Bacteriol. 1980 Jul;143(1):238–245. [PMC free article] [PubMed]
  • Negoro S, Taniguchi T, Kanaoka M, Kimura H, Okada H. Plasmid-determined enzymatic degradation of nylon oligomers. J Bacteriol. 1983 Jul;155(1):22–31. [PMC free article] [PubMed]
  • O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed]
  • Okada H, Negoro S, Kimura H, Nakamura S. Evolutionary adaptation of plasmid-encoded enzymes for degrading nylon oligomers. Nature. 1983 Nov 10;306(5939):203–206. [PubMed]
  • Ramos JL, Stolz A, Reineke W, Timmis KN. Altered effector specificities in regulators of gene expression: TOL plasmid xylS mutants and their use to engineer expansion of the range of aromatics degraded by bacteria. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8467–8471. [PubMed]
  • Rosenberg SM, Longerich S, Gee P, Harris RS. Adaptive mutation by deletions in small mononucleotide repeats. Science. 1994 Jul 15;265(5170):405–407. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)