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The microsurgical anatomy of the optic canal was defined on 20 cadaveric specimens. Anatomic parameters of the optic canal, optic nerve, ophthalmic artery, and adjacent structures were measured, and relations of these structures were noted. Five variants of the course of the ophthalmic artery relative to the optic nerve in the optic canal were found. Various aspects of microsurgery of the optic canal are discussed in relation to anatomic findings.