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Skull base surgery. 1997; 7(3): 123–128.
PMCID: PMC1656632

An Experimental Model of Intraoperative Venous Injury in the Rat


Intraoperative obliteration of cerebral veins occasionally causes unexpected severe complications, especially in elderly patients. However, very title information is available on the pathophysiology of cerebral venous circulation disturbance. Occlusion of cortical veins in rats by a photochemical thrombotic technique is a less invasive, clinically relevant and reproducible model that is suitable for the study of venous circulation disturbance. In the present study, 54 male Wistar rats were used. We examined changes of the cerebral venous flow pattern by fluorescence anglography and brain damage histologically in a one- or two-(cortical) vein occlusion model using a photochemical thrombotic technique. Approximately 30% (9 of 27) of animals in the single-vein occlusion group and 90% (15 of 17) of those in the two-vein occlusion group had microcirculation perturbation, which results very soon in the formation of venous thrombus accompanied by severe venous infarction. In addition, infarction size in the two-vein occlusion group (9.7 ± 3.2%) was significantly larger than in the single-vein group (2.9 ± 1.3%) (unpaired T-test, P < 0.01).

In conclusion, the photochemical dye technique of attaining cerebral venous occlusion is a worth while addition to the study of circulation perturbations of the brain.

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Selected References

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  • Fries G, Wallenfang T, Hennen J, Velthaus M, Heimann A, Schild H, Perneczky A, Kempski O. Occlusion of the pig superior sagittal sinus, bridging and cortical veins: multistep evolution of sinus-vein thrombosis. J Neurosurg. 1992 Jul;77(1):127–133. [PubMed]
  • Gotoh M, Ohmoto T, Kuyama H. Experimental study of venous circulatory disturbance by dural sinus occlusion. Acta Neurochir (Wien) 1993;124(2-4):120–126. [PubMed]
  • Nakase H, Kakizaki T, Miyamoto K, Hiramatsu K, Sakaki T. Use of local cerebral blood flow monitoring to predict brain damage after disturbance to the venous circulation: cortical vein occlusion model by photochemical dye. Neurosurgery. 1995 Aug;37(2):280–286. [PubMed]
  • Ungersböck K, Heimann A, Kempski O. Cerebral blood flow alterations in a rat model of cerebral sinus thrombosis. Stroke. 1993 Apr;24(4):563–570. [PubMed]
  • Dietrich WD, Feng ZC, Leistra H, Watson BD, Rosenthal M. Photothrombotic infarction triggers multiple episodes of cortical spreading depression in distant brain regions. J Cereb Blood Flow Metab. 1994 Jan;14(1):20–28. [PubMed]
  • Nakayama H, Dietrich WD, Watson BD, Busto R, Ginsberg MD. Photothrombotic occlusion of rat middle cerebral artery: histopathological and hemodynamic sequelae of acute recanalization. J Cereb Blood Flow Metab. 1988 Jun;8(3):357–366. [PubMed]
  • Prado R, Dietrich WD, Watson BD, Ginsberg MD, Green BA. Photochemically induced graded spinal cord infarction. Behavioral, electrophysiological, and morphological correlates. J Neurosurg. 1987 Nov;67(5):745–753. [PubMed]
  • Nakase H, Heimann A, Kempski O. Local cerebral blood flow in a rat cortical vein occlusion model. J Cereb Blood Flow Metab. 1996 Jul;16(4):720–728. [PubMed]
  • Nakase H, Heimann A, Kempski O. Alterations of regional cerebral blood flow and oxygen saturation in a rat sinus-vein thrombosis model. Stroke. 1996 Apr;27(4):720–728. [PubMed]
  • Frerichs KU, Deckert M, Kempski O, Schürer L, Einhäupl K, Baethmann A. Cerebral sinus and venous thrombosis in rats induces long-term deficits in brain function and morphology--evidence for a cytotoxic genesis. J Cereb Blood Flow Metab. 1994 Mar;14(2):289–300. [PubMed]
  • Nakase H, Kempski OS, Heimann A, Takeshima T, Tintera J. Microcirculation after cerebral venous occlusions as assessed by laser Doppler scanning. J Neurosurg. 1997 Aug;87(2):307–314. [PubMed]
  • Sakaki T, Kakizaki T, Takeshima T, Miyamoto K, Tsujimoto S. Importance of prevention of intravenous thrombosis and preservation of the venous collateral flow in bridging vein injury during surgery: an experimental study. Surg Neurol. 1995 Aug;44(2):158–162. [PubMed]

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