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Apoptosis of mature T lymphocytes preserves immune system homeostasis by counteracting transient increases in T-cell number. This process is regulated, at least in part, by the cytokine interleukin 2 (IL-2): T cells deprived of IL-2 undergo apoptosis. The mechanism of apoptosis induction by IL-2 deprivation remains to be determined but is known to require RNA synthesis, implying the existence of transcriptionally activated genes whose products induce cell death. To identify such genes, we have performed expression profiling in IL-2-dependent T cells following cytokine deprivation. Our results reveal an intricate transcriptional program entailing the induction of known proapoptotic factors and the simultaneous repression of known antiapoptotic factors. Surprisingly, one gene whose transcription substantially increased was RC3 (also called neurogranin), which encodes a calmodulin binding protein thought to be a neural-specific factor involved in learning and memory. We show that ectopic expression of RC3 in IL-2-dependent T cells increases the intracellular Ca2+ concentration and induces apoptosis even in the presence of cytokine. Buffering the Ca2+ increase with the cytoplasmic Ca2+ chelator BAPTA-AM [1,2-bis(2-aminophenoxy)ethane-N,N,N1,N-tetraacetic acid] blocks RC3-induced apoptosis, indicating that the rise in intracellular Ca2+ is required for apoptotic death. RC3 mutants unable to bind calmodulin fail to increase intracellular Ca2+ levels and to induce apoptosis. Based upon these results, we propose that IL-2 deprivation raises the level of RC3 and other apoptotic factors, which induce apoptosis by increasing the intracellular Ca2+ concentration.
A complex network of cytokines controls the development, maturation, homeostasis, and responses of the immune system. An immune response typically involves a large increase in activated T cells, which must be eliminated following termination of the response to preserve homeostasis. This is achieved by apoptosis (also called programmed cell death), which can result from either cytokine deprivation or T-cell receptor activation (19). The cytokine interleukin 2 (IL-2) is produced and secreted by activated T cells and controls lymphocyte survival and cell cycle progression (17), and paradoxically it can also facilitate a particular apoptotic program in T lymphocytes termed activation-induced cell death (25). When deprived of IL-2, cycling T cells cease to proliferate and rapidly undergo apoptosis (19).
A key component of the apoptotic machinery is a proteolytic system involving an evolutionarily conserved family of cysteine aspartate proteases called caspases. Although this machinery is common to many forms of apoptosis, the precise mechanisms by which it becomes activated can vary considerably. Two pathways leading to caspase activation have been characterized: the extrinsic pathway, which involves so-called death receptors, and the intrinsic pathway, which involves the release of proapoptotic proteins from the mitochondria. In addition, some apoptotic pathways are transcription dependent, whereas others entail a purely posttranscriptional signal transduction cascade.
There have been several proposals for the mechanism by which IL-2 deprivation leads to apoptosis, including regulation of Bcl-2 family members, modulation of second messengers, and alteration of the activity of protein kinases or phosphatases (1, 5, 11). However, none of these regulatory events explain the requirement of de novo RNA or protein synthesis for induction of apoptosis following IL-2 deprivation (10). Here we use expression profiling to identify death-promoting genes that are transcriptionally activated in lymphocytes following IL-2 deprivation.
RC3 cDNA was hemagglutinin tagged at the 3′ end and cloned into pcDNA3.1+ and pcDNA3.1− vectors (Invitrogen). IL-2-dependent murine HT-2 (T helper) cells, CTLL-2 (cytotoxic T) cells, myeloid 32D cells, HEK-293 cells, and human T-cell leukemia Jurkat cells were all obtained from the American Type Culture Collection; IL-3-dependent murine FL5.12 cells (pro-B lymphocytes) were provided by Craig Thompson (University of Pennsylvania). HT-2 and CTLL-2 cells were maintained in RPMI 1640 medium (GIBCO-BRL) supplemented with 10% fetal bovine serum (FBS), 2 mM l-glutamine (GIBCO-BRL), 1 mM sodium pyruvate (GIBCO-BRL), 100 U of penicillin (Sigma) per ml, 100 μg of streptomycin (Sigma) per ml, 0.05 mM 2-mercaptoethanol (GIBCO-BRL), and 200 U of recombinant IL-2 (PharMingen) per ml or 10% rat T-STIM factor with concanavalin A (Becton Dickinson). FL5.12 and 32D cells were maintained in RPMI medium supplemented with 10% FBS, 1 mM l-glutamine, 100 U of penicillin per ml, 100 μg of streptomycin per ml, 0.05 mM 2-mercaptoethanol, and 3 ng of recombinant IL-3 (PharMingen) per ml. HEK-293 cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% FBS, 1 mM l-glutamine, 100 U of penicillin per ml, and 100 mg of streptomycin per ml. Jurkat cells were maintained in RPMI medium supplemented with 10% FBS, 1 mM l-glutamine, 100 U of penicillin per ml, and 100 mg of streptomycin per ml.
Human peripheral blood lymphocytes from healthy donors were initially cultured in RPMI medium supplemented with 10% FBS, 1 mM l-glutamine, and 5 μg of phytohemagglutinin (Sigma) per ml for 2 days. Cells were then collected and placed in RPMI containing 10% FBS and 10% human IL-2 (Advanced Biotechnologies) for 4 days with a medium change every 48 h. Staurosporine (Sigma) and thapsigargin (Calbiochem) were added to the media to final concentrations of 10 and 2 μM, respectively. BAPTA-AM [1,2-bis(2-aminophenoxy)ethane-N,N,N1,N-tetraacetic acid] (Sigma) was added to the media to a final concentration of 25 μM.
HT-2 cells were cultured in the presence of IL-2 and subjected to IL-2 deprivation as described previously (10). Poly(A)+ mRNA was isolated at 8 h following IL-2 withdrawal by using an Oligotex direct mRNA isolation kit (Qiagen). The integrity of mRNA was monitored by a Northern blot assay with a glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) probe. Two micrograms of mRNA was used to generate a cDNA library by using an oligo(dT) T7 primer and Superscript cDNA synthesis system (GIBCO-BRL) according to the manufacturer's instructions. The cDNA library was in vitro transcribed with biotinylated nucleotides by using the T7 Megascript kit (Ambion) according to the manufacturer's instructions. The resulting cRNA was fragmented by heating at 94°C for 30 min. GeneChip Murine 19K and 11K Affymetrix oligonucleotide arrays representing ~30,000 known genes or expressed sequence tags were hybridized with fragmented cRNA overnight at 37°C. Following washing, the chips were scanned and analyzed with Affymetrix microarray suite software. The complete data set is available upon request.
HT-2, CTLL-2, and FL5.12 cells were transfected with plasmids by using Superfect transfection reagent (Qiagen) according to the manufacturer's instructions. Cells were collected 24 h after transfection and stained with annexin V-fluorescein isothiocyanate (FITC)-propidium iodide (Oncogene). For cotransfection experiments, HT-2 cells were cotransfected with 1 μg of enhanced green fluorescent protein (GFP) plasmid (Clontech) and 4 μg of pcDNA3.1+/RC3 (sense orientation) or pcDNA3.1−/RC3 (antisense orientation). Cotransfected cells were stained with annexin V-phycoerythrin (PE) (PharMingen) and analyzed by fluorescence-activated cell sorting (FACS) analysis. At least two independent experiments were performed, and the results are presented as the averages ± standard deviations.
For HEK-293 fibroblasts, cells were seeded into a Lab-Tek II chamber slide (Nunc) at 6 × 105 cells per slide and cotransfected with 0.5 μg of β-galactosidase reporter plasmid (Clontech) and 4 μg of expression plasmid (as indicated), using Superfect transfection reagent. Following transfection, cells were washed three times with phosphate-buffered saline, fixed with 0.5% glutaraldehyde, and stained with 0.2% 5-bromo-4-chloro-3-indolyl-β-d-galactoside (X-Gal) (Promega) as described previously (20). The percentage of transfected (blue) cells with apoptotic morphology was determined. At least two independent experiments were performed, and the results are presented as the averages ± standard deviations.
For DNA fragmentation analysis, DNA from 2 × 106 cells was isolated by phenol extraction 24 h following transfection and analyzed on a 1% agarose gel as previously described (32).
To measure intracellular Ca2+ concentrations, 5 × 106 HT-2 cells were stained with 2 mM fura-2 AM (Molecular Probes), a fluorescent Ca2+ indicator, in 1 ml of saline containing 1 mM CaCl2. Cells were stained for 30 min at 37°C and for 10 min at room temperature. The cells were washed two times with saline containing 1 mM CaCl2 and resuspended at 106 cells/ml in the same solution. Spectrometric analysis was performed at 380 and 340 nm as described previously (36). The Ca2+ ionophore ionomycin was added to a final concentration of 10 mM at the end of each experiment to determine whether the limits of saturation of fura-2 AM had been reached. At least two independent experiments were performed, and the results are presented as the averages ± standard deviations.
Intracellular Ca2+ was measured in primary human T lymphocytes and in HT-2 cells transfected with the RC3 expression plasmid. HT-2 cells were cotransfected with 5 μg of either pcDNA3.1+/RC3 or pcDNA3.1 and a plasmid expressing the truncated human CD4 molecule as a marker to select transfected cells. Transfected cells were then isolated from untransfected cells by using the MACSelect-transfected cell isolation kit (Miltenyi Biotech) according to the manufacturer's instructions. Transfected cells were then stained with 3 μM fura-2 AM in RPMI for 20 min at room temperature. Cells were washed three times with RPMI and plated onto poly-d-lysine-coated glass-bottom dishes (Mattek Corporation) for 10 min at room temperature. Cells were washed once with Ringer lactate solution. Images were collected at 340- and 380-nm wavelengths. Single-cell measurements of intracellular Ca2+ ion concentration were calculated from the 340 nm/380 nm ratios (generated from background-corrected 340 nm/380 nm images) by using the equation of Grynkiewicz et al. (18) and a Kd of 250 nM for fura-2 AM. At least two independent experiments were performed, and the results are presented as the averages ± standard deviations.
The mouse HT-2 T helper cell line requires IL-2 for growth, and in its absence HT-2 cells undergo apoptosis (Fig. (Fig.1A)1A) (34). For expression profiling, HT-2 poly(A)+ mRNA was isolated 8 h after IL-2 withdrawal and used to interrogate Affymetrix DNA chips that contained ~30,000 genes or expressed sequence tags. Comparison of the transcription profiles of cells grown in the presence or absence of IL-2 revealed that ~97% of the genes were unaffected by cytokine deprivation (Fig. (Fig.1B).1B). Of the genes whose transcription was altered by cytokine deprivation, surprisingly, approximately three-quarters were stimulated and one-quarter were repressed (Fig. (Fig.1C1C).
Several of the genes that underwent significant transcriptional changes following IL-2 deprivation are listed in Table Table1.1. Three of the genes that underwent transcriptional stimulation were CAPN5, encoding a calpain cysteine protease; DRP-1, which encodes death-associated protein kinase (DAPK)-related protein 1 (21); and RC3/neurogranin, encoding a calmodulin (CaM) binding protein (4). The apoptotic roles of calpains and DRP-1 are well documented (21, 24). DRP-1 encodes a CaM-regulated serine/threonine kinase that is highly similar to DAPK. Both kinases contain a CaM binding domain, and, interestingly, ectopic expression of either DRP-1 or DAPK induces apoptosis in a wide variety of cell types (6, 21).
Conversely, transcription of a number of genes decreased following IL-2 deprivation, including the Pim1 oncogene (33) and ATFx, which encodes a basic-region leucine zipper (bZIP) transcription factor (28) (Table (Table1).1). Significantly, expression of Pim1 and ATFx is also repressed following cytokine deprivation in IL-3-dependent FL5.12 cells (8), and both genes encode proteins with antiapoptotic activities (30, 35).
The finding that RC3 was transcriptionally activated in lymphocytes was surprising, because the RC3 protein was previously characterized as a neurally specific factor involved in synaptic plasticity and spatial learning (29, 37). Therefore, RC3 represented a putative novel gene involved in lymphocyte apoptosis, and we sought to test this hypothesis and study the mechanism of RC3 action. Northern blot analysis confirmed the results of the DNA microarray experiments: IL-2 withdrawal resulted in transcriptional activation of RC3 and repression of ATFx (Fig. (Fig.2A).2A). A time course analysis revealed that RC3 transcriptional induction preceded the onset of apoptosis (Fig. (Fig.2B).2B). Immunoblot analysis showed that like the mRNA levels, RC3 protein levels were also up-regulated following IL-2 withdrawal (Fig. (Fig.2C2C).
To investigate the specificity of RC3 transcriptional activation, we analyzed several other cytokine-dependent cell lines: IL-2-dependent CTLL-2 cells, IL-2-dependent primary human T lymphocytes, IL-3-dependent murine pro-B lymphocytic FL5.12 cells, and IL-3-dependent murine myeloid 32D cells. Similar to the results obtained with the IL-2-dependent HT-2 cell line, transcription of RC3 was also activated in CTLL-2 cells (Fig. (Fig.3A)3A) and in IL-2-dependent primary human T lymphocytes (Fig. (Fig.3B)3B) following cytokine deprivation. However, the results with the IL-3-dependent cell lines differed. Although RC3 mRNA could be detected in FL5.12 cells grown in the presence of IL-3, the mRNA levels were not significantly increased following cytokine deprivation (Fig. (Fig.3C).3C). In IL-3-dependent 32D cells, RC3 mRNA was not detected in either the presence or absence of cytokine (Fig. (Fig.3D).3D). Transcriptional activation of RC3 also did not occur in cytokine-deprived IL-7-dependent D1-F4 cells or serum-deprived human fibroblasts (data not shown). Finally, we analyzed RC3 expression in a cytokine-independent T-cell line (human T-cell leukemia Jurkat cells). Apoptosis was induced in Jurkat cells with staurosporine, and RC3 protein levels were analyzed by immunoblot analysis. Although staurosporine treatment resulted in proteolytic activation of the apoptotic marker caspase 3, expression of RC3 was not detected (Fig. (Fig.3E).3E). Collectively, these results indicate that RC3 transcriptional activation is not a general consequence of apoptosis induction due to growth factor deprivation.
To investigate whether overexpression of RC3 was sufficient to induce apoptosis, we transfected IL-2-dependent HT-2 and CTLL-2 cells and IL-3-dependent FL5.12 cells with a plasmid expressing RC3. After 24 h, cells were stained with annexin V-FITC-propidium iodide to monitor apoptosis. Figure Figure4A4A shows that expression of RC3 induced apoptosis in HT-2 and CTLL-2 cells but not in FL5.12 cells. As expected, transfection of cells with the expression vector pcDNA3.1 had no effect on either cell line. Immunoblot analysis revealed that the differential response of HT-2 and FL5.12 cells was not due to variations in RC3 expression (Fig. (Fig.4B4B).
The ability of RC3 to induce apoptosis in HT-2 cells was also investigated by using two other independent assays. In one assay, the RC3 expression plasmid was cotransfected with a plasmid expressing GFP, which provided a marker for transfected cells. Quantitation of cells that were both GFP positive and annexin V-PE positive by FACS analysis confirmed that ectopic RC3 expression induced apoptosis (Fig. (Fig.4C).4C). As expected, cotransfection with a plasmid lacking the RC3 coding sequence (pcDNA3.1 vector alone) or with RC3 cloned in the antisense orientation failed to induce apoptosis (Fig. (Fig.4C).4C). In the second assay, DNA extracted from HT-2 and CTLL-2 cells transfected with an RC3 expression plasmid was analyzed by using a DNA fragmentation assay. Figure Figure4D4D shows that in both cell types RC3 expression resulted in a characteristic DNA ladder, confirming that cell death was apoptotic.
Alterations in intracellular ion levels are a feature of several apoptotic pathways, and the involvement of Ca2+ has been well documented. For example, Ca2+ ionophores, which directly mobilize Ca2+ from internal stores, induce apoptosis in diverse cell types (27). Conversely, agents that decrease intracellular Ca2+ concentration can inhibit apoptosis; for example, one of the many activities of the antiapoptotic Bcl-2 protein is to prevent the loss of Ca2+ from intracellular stores, thereby blocking apoptosis (2).
Many of the physiological effects of Ca2+ are mediated by CaM, a small, ubiquitous protein that regulates a wide variety of cellular processes by activating Ca2+/CaM-dependent enzymes that, in turn, modulate signaling cascades. Like intracellular Ca2+, CaM may also regulate apoptosis; for example, increased CaM expression has been correlated with apoptosis (9), and, conversely, CaM antagonists can interfere with apoptosis (26).
RC3 contains a well-characterized 20-amino-acid sequence known as the IQ motif (3), which functions as a binding site for CaM (12). Specific single amino acid substitutions in the IQ domain eliminate binding of RC3 to CaM in vivo (31). In the brain, CaM appears to be the major protein that interacts with RC3, and the RC3-CaM interaction is believed to be essential for RC3 function (12, 31). To investigate whether the ability of RC3 to induce apoptosis correlated with its ability to bind CaM, we analyzed the apoptotic activity of RC3 derivatives bearing mutations in the CaM binding domain by using two different assays. In the first assay, HT-2 cells cultured in the presence of IL-2 were transfected with plasmids expressing either wild-type RC3 or an RC3 mutant, and the percentage of apoptotic cells was determined by annexin V-FITC staining. Figure Figure5A5A shows that mutation of Ile-33 to Gln (I33Q), which completely inhibits RC3 binding to CaM (31), also eliminated its proapoptotic activity. Similarly, mutation of either Ser-36 to Asp (S36D) or Arg-38 to Gln (R38Q), which weakens the RC3-CaM interaction (31), also reduced the proapoptotic activity of RC3. In contrast, the Ser-36-to-Ala mutation (S36A), which does not affect the RC3-CaM interaction (31), induced apoptosis at levels similar to those for wild-type RC3.
In the experiment shown in Fig. Fig.5A,5A, apoptosis was analyzed in a population in which there was a high background of untransfected cells (~60%). To confirm these results, we performed a second assay that measured apoptosis only in transfected cells. For these experiments, which involve staining individual cells, we used the adherent cell line HEK-293. HEK-293 cells were cotransfected with a β-galactosidase reporter plasmid and a plasmid encoding either the wild-type or mutant RC3 protein. The cells were then stained with the chromogenic substrate X-Gal, which marks transfected cells, and apoptotic cells were identified by their characteristic condensed morphology (Fig. (Fig.5B)5B) and quantitatively assessed (Fig. (Fig.5C).5C). The results of this second apoptotic assay were similar to those of Fig. Fig.5A.5A. Collectively, these results reveal a strong correlation between the abilities of RC3 to bind CaM and to induce apoptosis.
An increased intracellular Ca2+ concentration is known to induce apoptosis. We therefore reasoned that increased RC3 levels that occur during IL-2 deprivation might trigger apoptosis by sequestering CaM, thereby releasing Ca2+ and increasing the intracellular Ca2+ concentration. As an initial test of this hypothesis, we measured intracellular Ca2+ levels in HT-2 cells following IL-2 deprivation. As a positive control, HT-2 cells were treated with thapsigargin, which releases Ca2+ from intracellular pools and induces apoptosis. Figure Figure6A6A shows that thapsigargin treatment increased the intracellular Ca2+ concentration 10-fold, whereas IL-2 deprivation increased the intracellular Ca2+ concentration 5-fold. We conclude that IL-2 deprivation increases intracellular Ca2+ concentration.
We next asked whether RC3 expression would, like IL-2 deprivation, increase the intracellular Ca2+ concentration. HT-2 cells were transfected with a plasmid expressing RC3 or an RC3 mutant. Figure Figure6B6B shows that the intracellular Ca2+ concentration in cells transfected with an RC3 expression plasmid was 2.5-fold higher than that in untransfected cells or cells transfected with the pcDNA3.1 expression plasmid. After correcting for transfection efficiency (~40%), we concluded that RC3 expression increased the intracellular Ca2+ concentration approximately sixfold, which was very similar to the result observed following IL-2 deprivation. Analysis of two RC3 mutants (R38Q and S36A) again revealed a correlation between the abilities of RC3 to interact with CaM and to increase intracellular Ca2+. We conclude that RC3 increases the intracellular Ca2+ concentration.
To directly quantify the Ca2+ increase induced by RC3 expression, we performed single-cell imaging experiments. HT-2 cells transfected with either an RC3 expression plasmid or pcDNA3.1 were isolated and subjected to single-cell intracellular Ca2+ imaging analysis. The pseudocolor image in Fig. Fig.6C6C shows that RC3 expression resulted in an increased intracellular Ca2+ concentration. Quantitation revealed that RC3 expression increased the intracellular Ca2+ concentration approximately eightfold, comparable to the approximately ninefold increase observed upon IL-2 withdrawal in the same assay, confirming the results of Fig. Fig.6A6A and B. Finally, a similar analysis for IL-2-dependent primary human T lymphocytes again indicated that RC3 expression increased the intracellular Ca2+ concentration (Fig. (Fig.6D6D).
To determine if the Ca2+ increase that occurs following IL-3 withdrawal or RC3 expression is directly responsible for triggering apoptosis, we asked whether depletion of intracellular Ca2+ could protect cells from apoptotic death. Incubation in the presence of BAPTA-AM, an intracellular Ca2+ chelator, inhibited apoptosis induced by cytokine deprivation in IL-2-dependent HT-2 cells (Fig. (Fig.7A)7A) and in IL-2-dependent primary human T lymphocytes (Fig. (Fig.7B).7B). Similarly, BAPTA-AM treatment abolished the apoptotic effects of RC3 expression (Fig. (Fig.7C).7C). These results indicate that intracellular free Ca2+ accumulation plays a critical role in apoptosis induced by either IL-2 deprivation or RC3 expression.
We have found that induction of apoptosis due to cytokine deprivation in IL-2-dependent lymphocytes involves an orchestrated transcriptional program in which genes encoding proapoptotic factors are transcriptionally activated and genes encoding antiapoptotic factors are transcriptionally repressed. In addition, we have identified a novel proapoptotic factor, RC3, a member of the calpacitin family, whose members bind and release CaM in response to Ca2+ flux (13). The role of RC3 in modulating intracellular Ca2+ and CaM levels in neurons is well documented (13). Our studies reveal that RC3 also has a function in lymphocytes. We find that RC3 is transcriptionally activated in IL-2-dependent T cells following cytokine deprivation and that ectopic expression of RC3 in these cells induces apoptosis. These results suggest a model in which RC3 plays a role in eliminating activated T cells following an immune response. Consistent with a potential role in the immune response, previous studies have shown that in addition to the brain, RC3 is expressed in the spleen and thymus (16, 37). RC3 knockout mice have been derived (29), and it will be important to carefully evaluate their immunological status.
Although the mechanism by which IL-2 promotes transcription has been well characterized (reviewed in reference 15), the pathway by which IL-2 deprivation increases transcription of RC3 and other genes remains to be elucidated. One potential clue has come from studies with IL-2-dependent mouse lymphoid cells, which show that expression of the AP-1 transcription factors c-Fos and c-Jun is rapidly induced following growth factor deprivation (7). The RC3 promoter contains putative AP-1 binding sites (22), suggesting a possible mechanism for transcriptional activation of RC3 following IL-2 deprivation.
The proapoptotic activity of RC3 requires a functional CaM binding domain, indicating that the ability of RC3 to induce apoptosis is related to its role in modulating intracellular CaM and/or Ca2+ levels. Recent computer-based modeling of calpacitin-CaM interactions predicts that increased RC3 levels would lead to dissociation of the Ca2+-CaM complex, thereby increasing intracellular Ca2+ levels (14). Consistent with this prediction, we found that overexpression of RC3 resulted in an increased intracellular Ca2+ concentration (Fig. (Fig.66).
Based on the findings presented in this report and previous studies on calpacitin regulation and function, we propose the following model for the induction of apoptosis by RC3 in lymphoid cells. IL-2 deprivation induces RC3 expression, and the elevated levels of RC3 sequester CaM and stabilize it in its Ca2+-free form, which, as described above, contributes to an increase in intracellular Ca2+ and triggers apoptosis. In support of this model, antisense-mediated blocking of the Ca2+ channel renders T cells resistant to apoptosis, indicating that intracellular Ca2+ levels play a role in T-cell apoptosis (23).
IL-2 deprivation may also decrease protein kinase C (PKC) activity (17). PKC phosphorylates RC3 and blocks the RC3-CaM interaction (12). Thus, a reduction in PKC phosphorylation should stabilize the Ca2+-free form of CaM, which would also increase the intracellular Ca2+ concentration. Finally, RC3 could also increase intracellular Ca2+ and induce apoptosis by regulating the association of Ca2+/CaM with various proteins, thereby modulating other CaM-dependent pathways. One such protein may be the CaM-dependent plasma membrane Ca2+-ATPase; a decreased efflux of intracellular Ca2+ could contribute to the Ca2+ increase.
The ability of a CaM binding protein to induce apoptosis when overexpressed has also been demonstrated with two proapoptotic serine/threonine kinases: DAPK and DRP-1. Intriguingly, like that of RC3, expression of DRP-1 is also increased following IL-2 deprivation (Table (Table1).1). DRP-1 has been shown to induce apoptosis when expressed in a variety of cell lines (21), and both RC3 and DRP-1 have similar IQ motif CaM binding domains. It therefore appears that IL-2 deprivation increases the level of multiple CaM-interacting proteins that activate parallel apoptotic pathways, thus ensuring efficient onset of apoptosis.
We thank J. G. Sutcliffe for the RC3 cDNA and antibody, D. Storm for RC3 mutants, C. Sagerstorm for help with the microscopy, A. Earhardt and D. Lambright for help with the spectrofluorometry, the University of Massachusetts Medical School Center for AIDS Research (CFAR) for human peripheral blood lymphocytes, P. Furcinitti for help with the Ca2+ single-cell imaging, members of our laboratory for helpful discussions and comments on the manuscript, and S. Evans for editorial assistance.
This work was supported in part by an NIH grant (to M.R.G.) and by the Leukemia and Lymphoma Society (to L.R.D.). M.R.G. is an investigator of the Howard Hughes Medical Institute.