The 96-well plate is widely used in ELISpot assay. The whole plate information
will be broken into small pieces according to the normalization
of relational database, and saved into different tables. The overall
design concept of database tables for DBSEA can be shown below.
Standardized data entry
It is challenging to standardize the immunology lab data due to a great
variability and inconsistency in laboratory process and data management. The
DBSEA shall use the standardized data entry for the effort of
data standardization in ELISpot assay. Many lab investigators typically
use the Excel spreadsheet as a means of data recording for the plate
information in a format of 8×12 matrix (96 wells). It will be
very useful if we can create a general template in Excel spreadsheet
for lab investigators. Then, we let DBSEA do data entry automatically
by transferring data from spreadsheet to database tables.
The DBSEA will use a custom authentication schema to ensure data security. The
username and password will be required when logging to DBSEA. The
investigator can access his/her own data in DBSEA. The data can be
shared only after the original investigator issues permission for it.
The DBSEA is not just for a group of investigators connected by a local
network. It will mainly provide data services for broad research communities. As
a user-friendly Web application, the DBSEA will be effectively
used through the Internet for data saving, data retrieval, and data
Data sharing method
The DBSEA shall provide the mechanism for data sharing among broad research
communities. The data will be available for sharing once the original
investigator releases permission. The DBSEA shall let investigators
download the data in multiple methods, such as spreadsheet and XML
document. By downloading data in XML, investigators can use their own
tools to parse XML files and analyze data.