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Nuclear aggregates of polyglutamine (polyQ)-expanded proteins are associated with a number of neurodegenerative diseases including Huntington’s disease (HD) and spinocerebellar ataxias (SCAs). The nuclear deposition of polyQ proteins correlates with rearrangements of nuclear matrix, transcriptional dysregulation, and cell death. To explore the requirement for polyQ tracks in educing such cellular responses, we examined whether a non-polyQ protein can deposit as nuclear aggregates and elicit similar responses. We report that a protein chimera (GFP170*) composed of the green fluorescent protein (GFP) fused to an internal fragment of the Golgi Complex Protein (GCP-170) forms nuclear aggregates analogous to those formed by polyQ proteins. Like the polyQ nuclear aggregates, GFP170* inclusions recruit molecular chaperones and proteasomal components, alter nuclear structures containing the promyelocytic leukemia protein (PML), recruit transcriptional factors such as CREB-binding protein (CBP) and p53, repress p53 transcriptional activity, and induce cell death. Our results indicate that nuclear aggregation and transcriptional effects are not unique to polyQ-containing proteins and may represent a general response to misfolded proteins in the nucleus.
At least nine neurodegenerative diseases, including Huntington’s disease (HD), spinobulbar muscular atrophy (SBMA), dentatorubral-pallidoluysian atrophy (DRPLA), and spinocerebellar ataxias (SCA) 1, 2, 3, 6, 7, and 17, are caused by a single type of mutation, the expansion of CAG repeats encoding for a polyglutamine (polyQ) track in unrelated proteins (Zoghbi and Orr, 2000). The mutant proteins form protein aggregates or inclusions that are the hallmark of polyQ diseases (Ross, 2002). Unlike other mutant proteins that form cytoplasmic aggregates in diseases such as Alzheimer’ and Parkinson’s diseases or amyo-trophic lateral sclerosis, polyQ proteins can be deposited as cytoplasmic inclusions as well as nuclear inclusions. The nuclear deposition of polyQ proteins has been correlated with cytotoxicity. Transgenic mice expressing polyQ human huntingtin develop neuronal intranuclear inclusions prior to developing a neurological phenotype (Davies et al., 1997). Similarly, nuclear localization of polyQ proteins is essential to induce cell death in cultured cell and transgenic mouse models (Katsuno et al., 2002; Klement et al., 1998; Takeyama et al., 2002). However, the exact correlation between nuclear polyQ aggregates and pathology remains elusive. It is apparent that not all neurons containing polyQ aggregates die (Ross et al., 1998), and temporal studies of the appearance of aggregates and the onset of clinical pathology suggest that tissue damage and pathology can manifest before detection of aggregates (Saudou et al., 1998).
PolyQ pathogenesis may be linked to the sequestration and inactivation of proteins essential for cellular functions. Analyses of human post-mortem brains, animal models, and cell culture systems have shown that polyQ deposits recruit various cellular components. Invariably, proteins involved in protein folding and degradation, as well as transcriptional regulators are associated with polyQ aggregates (Li and Li, 2004). All the major classes of chaperones including members of the Hsp70 family (Hsc70 and Hsp70) and the Hsp40 family (Hdj1 and Hdj2) are recruited. Like chaperones, proteasomes have been shown to be associated with polyQ aggregates (Waelter et al., 2001). Often, polyQ inclusions are ubiquitin positive (DiFiglia et al., 1997). The sequestration of folding/degradative machinery to protein aggregates results in compromised proteasomal degradation (Bence et al., 2001).
Nuclear factors also have been shown to interact with polyQ nuclear inclusions (Okazawa, 2003). Nuclear aggregates of ataxin-1 recruit the promyelocytic leukemia protein (PML), a component of nuclear PML bodies (Skinner et al., 1997). Direct association of the CREB binding protein (CBP) with polyQ aggregates has been observed in HD cell culture models, HD transgenic mice, and human HD post-mortem brain (Nucifora et al., 2001). Sp1 and p53 also interact with polyQ huntingtin fragments (Nucifora et al., 2001; Steffan et al., 2000).
The sequestration of transcription factors by aggregates appears to alter their transcriptional activity. Specifically, polyQ expanded huntingtin and atrophin-1 (responsible for DRPLA) decrease CBP-mediated transcription in transfected primary cortical neurons (Nucifora et al., 2001). Similarly, polyQ expanded huntingtin represses transcription of a p53 reporter construct (Steffan et al., 2000). Genomic screens have shown that CBP-regulated genes, such as eukepholin and Jun, are downregulated in HD transgenic mice and in HD post-mortem brains (Luthi-Carter et al., 2000; Richfield et al., 1995). The inhibition of transcription may be a consequence of direct binding since CBP and p53 interact directly with polyQ tracks of huntingtin (Nucifora et al., 2001; Steffan et al., 2000) and with the polyQ tracks of androgen receptor that causes SBMA (McCampbell et al., 2000). Transcriptional regulators such as CBP and TATA binding protein (TBP) contain polyQ stretches, suggesting that complementary polyQ–polyQ interactions may mediate the sequestration and the inactivation.
The ability of nuclear polyQ aggregates to recruit folding/degradative cellular components, disrupt nuclear architecture, sequester transcriptionally relevant proteins, and alter transcriptional activity of the sequestered factors may be specific to the polyQ content or may represent a general cellular response to nuclear inclusions. To address this question, it is necessary to examine whether non-polyQ proteins can form nuclear inclusions and elicit similar cellular effects as polyQ aggregates. Here, we document that a non-polyQ protein (GFP170*), which contains GFP fused to an internal segment (amino acids 566 to 1375) of the Golgi Complex Protein 170 (GCP170), forms nuclear aggregates analogous to those deposited by polyQ proteins. GCP-170, also known as golgin-160, is a Golgi localized protein that associates peripherally with the cytoplasmic side of Golgi membranes (Hicks and Machamer, 2002; Misumi et al., 1997). GCP-170 was identified as a Golgi auto-antigen in sera of patients suffering from Sjorgen Syndrome (Fritzler et al., 1993). The cellular function of GCP170 is currently unknown. The internal fragment of GCP170 used here to generate GFP170* represents an artificial substrate that lacks a polyQ-expanded sequence. The ability of GFP170* to form nuclear aggregates suggests that the formation of nuclear inclusions is not a polyQ-specific process. Furthermore, like deposits of polyQ proteins, the nuclear aggregates of GFP170* recruit molecular chaperones and proteasomal components, cause a redistribution of PML bodies, and sequester transcription factors such as CBP and p53. In addition, expression of GFP170* represses p53 transcriptional activity and causes cell death. The similarity in cellular responses elicited by polyQ proteins and our non-polyQ GFP170* is consistent with the hypothesis that those responses are common to the presence of any misfolded proteins in the nucleus. Our findings raise the possibility that the etiology of diverse polyQ diseases such as HD, SBMA, DRPLA, and ataxias may share a set of common cytopathologies elicited solely by nuclear inclusions (irrespective of the polyQ content of the protein), in addition to specific responses elicited by distinct polyQ proteins.
Polyclonal anti-GFP antibody was from Abcam Inc. (Cat. # AB-290). Anti-CBP (A-22) (Cat. # sc-369) polyclonal antibody and anti-SC35 (Y-16) (Cat. # sc-10251) monoclonal antibody were purchased from Santa Cruz Biotechnology, Inc. Anti-Hsp70 (Cat. # SPA-810) monoclonal antibody was purchased from Stressgen Biotechnologies. Anti-Hdj2 polyclonal antibody was a gift from Dr. Douglas Cyr (University of North Carolina at Chapel Hill). Anti-20S proteasome (α-subunit) polyclonal antibody was purchased from Calbiochem-Novabiochem. Mouse anti-GMP-1 monoclonal antibody (clone 21C7) recognizes the SUMO-1 protein was from Zymed Laboratories Inc. A combined monoclonal anti-p53 antibody, 1801 and 421, was kindly provided by Dr. Xinbin Chen (University of Alabama at Birmingham). Texas red-labeled goat anti-mouse IgG antibody, Texas red-labeled goat anti-rabbit IgG antibody, and Hoechst 33258 were from Molecular Probes, Inc. Restriction enzymes and molecular reagents were from Promega, New England BioLabs, Inc., or QIAGEN. All other chemicals were from Sigma-Aldrich or Fisher Scientific.
To make a chimera of GFP and GCP-170, an XhoI restriction enzyme site was generated in front of the start codon of GCP-170. The 770-base pair PCR fragment containing sequences from the start codon of GCP-170 to the EcoRI site of FQSY1024 (Misumi et al., 1997) was cloned into the XhoI and EcoRI sites of pEGFP-C2 plasmid (Clontech Laboratories Inc.). A 5558-base-pair EcoRI fragment from FQSY1024 was then cloned to the EcoRI sites of the plasmid above to generate an EGFP-tagged full-length GCP-170 (GFP-GCP170FL). GFP170* construct was then generated by removing the BglII fragment and SacII fragment from the N-terminal and C-terminal end of GCP-170, respectively. The resulted construct expresses an EGFP-tagged GCP-170 fragment from amino acid 566 to 1375. The Q80-GFP construct has been described previously (Ding et al., 2002) and was kindly provided by Dr. Qunxing Ding (University of Kentucky). The construct expressing firefly luciferase under the control of the p21 promoter with two p53-responsive elements was provided by Dr. Xinbin Chen and has been described previously (Chinery et al., 1997).
COS-7 cells were grown in DMEM with glucose and glutamine (Mediatech, Inc.) supplemented with 10% FBS (Life Technologies), 100 U/ml penicillin, and 100 μg/ml streptomycin (Life Technologies). Cortical neurons isolated from mouse were cultured in Neurobasal Media (Cat.#21103-049, GIBCO) supplemented with B27 (Cat.#17504-010, GIBCO). Cells were transfected with the Fugene transfection reagent (Roche) or with TransIT polyamine transfection reagents (Mirus Corporation), according to manufacturer protocols. 18–48 h after transfection, cells were fixed with 3% paraformaldehyde and processed for immunofluorescence microscopy as previously described (Alvarez et al., 1999).
Cells were transfected with the GFP170* construct or the Q80-GFP construct. 48 h after transfection, cells were washed with PBS, detached from the plate by trypsinization, and collected by centrifugation at 300 × g for 5 min at 4°C. Cells were washed twice with PBS and then fixed for 90 min with 1.5% glutaraldehyde in 0.1 M sodium cacodylate pH 7.4. Cells were then washed three times with sodium cacodylate and postfixed with 1% OsO4 in 0.1 M sodium cacodylate pH 7.4 for 60 min on ice. After washing three times with 0.1 M sodium cacodylate pH 7.4, cells were dehydrated with a series of ethanol solutions (30, 50, 70, 90, 95, and 3 × 100%) followed by 2 h incubation in 1:1 Spurr’s resin/propylene oxide. After two changes of fresh 100% resin, the cell pellets were transferred to gelatin molds and polymerized in fresh resin overnight at 60°C. Gold epoxy sections (100 nm thick) were generated with a Reichert Ultracut ultramicrotome and collected on 200 mesh copper grids. The grid specimens were stained for 20 min with saturated aqueous uranyl acetate (3.5%) diluted 1:1 with ethanol just before use followed by staining with lead citrate for 10 min. Stained samples were examined on a JEOL 100CX electron microscope.
For immunogold electron microscopy, cells expressing GFP170* were harvested by trypsinization 24 h after transfection. Cells were washed with PBS and pre-fixed with 3% formaldehyde and 0.2% glutaraldehyde for 40 min followed by dehydration with series of graded ethanol at room temperature. The cells were then infiltrated and embedded with LR white. After polymerization, sections were cut with ultramicrotome and collected onto nickel grids. The grids were incubated with anti-GFP primary antibody and goat anti-rabbit IgG conjugated to 6-nm gold particles (Jackson ImmunoResearch Laboratories, Inc.) followed by postfixation with 2% glutaraldehyde and counterstaining with uranyl acetate. Samples were then examined on a JEOL 100CX electron microscope.
COS-7 cells were either mock-transfected with PBS or transfected with GFP170* construct. 48 h after transfection, cells were washed and harvested in ice-cold PBS. Cells were then lysed for 1 h on ice with RIPA buffer (50 mM Tris–HCl, pH 8.0, 1% NP-40, 0.5% deoxycholate, 0.1% SDS, and 150 mM NaCl) supplemented with protease inhibitor cocktail and 1.0 mM PMSF. Lysates were sonicated for 5 s with microtip sonicator followed by 15 min centrifugation at 15,000 × g. Pellets were washed 2× with RIPA buffer and resuspended with equal volume of RIPA buffer. Equal volumes of samples from the total cell lysate, supernatant, and pellet fractions were boiled in SDS-PAGE sample buffer and resolved on 8% SDS-PAGE. The gel was transferred to nitro-cellulose membrane and processed for Western blotting as previously described (Gao and Sztul, 2001).
COS-7 cells were transfected with either GFP170* or pEGFP-C2 (BD Bioscience). 32 h after transfection, the cells were incubated with 30 μM BrdU for 14 h followed by immunofluorescent staining with anti-BrdU monoclonal antibody, PRB-1 (Molecular Probes).
COS-7 cells were mock-transfected or transfected with GFP170* or Q80-GFP. 48 h after transfection, cells were detached from the plate by trypsinization. Cells were then incubated with a red fluorescent dye L-23102 for 30 min at room temperature. Live cells exclude the dye and therefore can be separated from dead cells based on their low fluorescence intensity. Cells were then fixed with formaldehyde and washed with PBS followed by FACS analysis. Cells were first gated according to the intensity of green fluorescence. Dead cells in GFP-negative or GFP-positive groups were counted separately.
COS7 cells in 6-well plates were transfected with 300 ng luciferase expressing vector and 300 ng of pcDNA3.1 vector alone or vector expressing Q80-GFP or GFP170*. 48 h after transfection, cell lysates were made using the passive lysis buffer in the Dual Luciferase Assay system form Promega according to the manufacturer’s instructions. Luciferase activity in the lysate was measured with a Luminometer from Promega. The protein concentrations of the lysates were determined by Bradford analysis, and luciferase activity was calculated per milligram of protein and then normalized to the activity in the control sample.
GCP-170 contains 1530 amino acids, arranged into an N-terminal head domain followed by a long stalk regions and a short C-terminal tail. The stalk region consists of 6 coiled-coil domains. The coiled-coil domains of GCP170 may be responsible for its dimerization (Hicks and Machamer, 2002; Misumi et al., 1997). Coiled-coil domains are known to mediate protein–protein interactions and may enhance the propensity of a protein to aggregate. GCP170 has been shown to be aggregation-prone in vitro (Misumi et al., 1997). In agreement, GFP-tagged full-length GCP-170 protein (GFP-GCP170FL) forms aggregates when over-expressed in COS-7 cells (Fig. 1A, insert, arrows).
Here, we have generated a chimera by fusing in frame an internal segment of the coiled-coil region of GCP170 composed of amino acids 566 to 1375 to the C-terminus of the enhanced green fluorescent protein (GFP). The resulting construct is called GFP170*. GFP170* does not contain polyQ repeats (Misumi et al., 1997). When transiently expressed in COS-7 cells, GFP170* deposits as cytoplasmic aggregates in the peri-nuclear region (Fig. 1A, arrows). The aggregates appear “ribbon-like” and are significantly more dispersed than the ‘‘ball-like’’ aggregates formed by GFP-250 (Garcia-Mata et al., 1999) or CFTR (Johnston et al., 1998). The GFP170* aggregates appear concentrated around the nucleus, but in some cases extend into the periphery of the cell. In addition to the cytoplasmic inclusions, GFP170* deposits in spherical foci within the nucleus (Fig. 1A, arrowheads). The morphology of cytoplasmic and nuclear GFP170* aggregates was compared to those formed by a model polyQ protein (Q80-GFP) (Fig. 1B). Q80-GFP encodes a fusion protein containing an 80-glutamine expansion fused to the amino-terminus of GFP. Q80-GFP has been shown to deposit in characteristic cytoplasmic and nuclear aggregates (Onodera et al., 1997). The cytoplasmic inclusions formed by Q80-GFP are irregular in shape (arrow), while the nuclear aggregates are spherical (arrowheads) and resemble the GFP170* aggregates. The Q80-GFP cytoplasmic inclusions localize to the peri-centriolar region but appear more compact than those of GFP170*. Q80-GFP forms one or two aggregates per nucleus, while GFP170* forms multiple inclusions per nucleus.
We examined the ultrastructure of GFP170* aggregates by transmission electron microscopy (Fig. 1C). The cytoplasmic aggregates (arrows) can extend to more than 15 μm in length. They are often surrounded by mitochondria, similar to the close association of mitochondria with the cytoplasmic aggregates formed by the HDQ83 huntingtin mutant (Waelter et al., 2001). The nuclear aggregates of GFP170* (arrowheads) are spherical or ovoid and range from 0.5 μm to 3 μm in diameter. They are similar to the nuclear inclusions formed by the Q80-GFP (Fig. 1D). In both cases, the nuclear aggregates appear as homogenous accumulations of granular material, without apparent fibrillar content or subdomain structures. Non-transfected control cells never contain cytoplasmic or nuclear aggregates (data not shown). The deposition of GFP170* within the morphologically defined cytoplasmic and nuclear aggregates was confirmed by immunogold labeling with anti-GFP antibodies. Gold particles label the cytoplasmic and nuclear aggregates (Figs. 1E and F).
The clinically relevant deposits of polyQ proteins occur in neuronal cells (Ross, 2002). To test if GFP170* aggregates also form in neuronal cells, we expressed GFP170* in mouse primary cortical neurons. Q80-GFP was used in analogous transfections to allow direct comparisons. As shown in Fig. 2, both GFP170* and Q80-GFP form cytoplasmic and nuclear aggregates in cultured mouse primary cortical neuronal cells. Their morphologies are similar. Like in COS-7 cells, GFP170* forms multiple nuclear inclusions, while Q80-GFP deposits within a single structure. Similar results were also obtained with PC12 cells, a rat neuronal cell line (data not shown). These results indicate that GFP170* forms aggregates in neuronal cells that are morphologically indistinguishable from those formed in non-neuronal cells. This suggests that results obtained in COS-7 cells may be applicable to neuronal cells. In our studies, COS-7 cells are used because of their ease of culture and transfection.
A characteristic feature of polyQ aggregates that parallels cytopathology is the recruitment of various cellular components. Specifically, polyQ aggregates have been shown to recruit molecular chaperones and proteasomes. Inclusions of polyQ-expanded huntingtin recruit proteasomal subcomplexes 20S, 11S, and 19S and the chaperones BIP, HSP70, and HSP40 (Waelter et al., 2001). Similarly, polyQ-expanded androgen receptor aggregates recruit HSP70 (Kobayashi et al., 2000). This may facilitate the degradative clearance of the aggregates (Cummings et al., 1998). Therefore, we examined the recruitment of similar components by GFP170* aggregates. Hsp70 and Hdj2, representatives of the Hsp70 and the Hsp40 families of chaperones, respectively, are recruited to the nuclear as well as cytoplasmic GFP170* deposits (Figs. 3A and B). In addition to chaperones, proteasomal components are also recruited to the cytoplasmic and nuclear GFP170* aggregates (Fig. 3C). Another important feature of the polyQ aggregates is that they are usually detergent insoluble (Perez et al., 1998; Waelter et al., 2001). We examined the solubility of GFP170* aggregates after lysing cells in buffer containing detergent. GFP170* is largely insoluble in a RIPA buffer containing 0.1% SDS (Fig. 3D). The recovery of cytosolic β-tubulin in the soluble fraction provides an internal control for the efficacy of the solubilization.
Nuclear inclusions of polyQ proteins have been shown to recruit nuclear structures containing the promyelocytic leukemia protein (PML bodies) (Skinner et al., 1997). PML bodies are also called nuclear domain 10 (ND10) bodies or PML oncogenic domains (PODs). The mammalian nucleus contains 10 to 30 PML bodies, which vary in size from 0.2 to 1 μm. They are thought to function in transcriptional regulation, cell cycle progression, and apoptosis, based on their content of proteins such as Sp100, PML, Daxx, pRB, CBP, and p53 that are involved in these processes (Maul et al., 2000; Yasuda et al., 1999; Zhong et al., 2000). Disruption of PML bodies caused by the (t15;17:q22;q21) translocation that results in a fusion of the PML protein and the retinoic acid receptor alpha (RARα) leads to acute promyelocytic leukemia (Weis et al., 1994).
GFP170* aggregates also recruit PML bodies (Figs. 4A and B). In normal untransfected cells, PML bodies are detected as numerous small nuclear foci (Figs. 4A and B, arrowheads). PML bodies with similar morphology are also evident in a cell expressing GFP170* at low levels (Fig. 4A, arrows). A distinct phenotype is observed in cells expressing high levels of GFP170* and displaying large nuclear aggregates (Fig. 4B). In such cells, PML bodies re-distribute to the surface of the GFP170* aggregates (Fig. 4B, arrows). Our results indicate that, like polyQ proteins, GFP170* causes changes in the nuclear architecture of PML bodies.
Recently, it has been shown that the mutant huntingtin, Httex1p, is modified with the small-ubiquitin-related modifier (SUMO) (Steffan et al., 2004). Like ubiquitin, SUMO is ligated to lysine residues of a variety of proteins involved in multiple cellular pathways (Verger et al., 2003). For example, the PML protein in the PML bodies is sumoylated (Duprez et al., 1999). We therefore tested the relationship between GFP170* aggregates and SUMO-1. SUMO-1 appears diffusely distributed in the nuclei of control cells (Fig. 4C, arrows). SUMO-1 appears to be recruited to the GFP170* nuclear aggregates, but not the cytoplasmic GFP170* aggregates in cells expressing GFP170* (Fig. 4C, arrows). The insert shows extensive co-localization of GFP170* and SUMO-1 in the nuclear aggregates.
To explore whether GFP170*, like Httex1p, is sumoylated, we performed immunoblotting experiments. GFP170* is detected as a ~124-kDa band in transfected cells (Fig. 4D, anti-GFP panel), and this protein is also detected by anti-SUMO-1 antibodies (anti-SUMO panel). A major sumoylated ~98-kDa band detected in non-transfected and in transfected cells corresponds in molecular weight to sumoylated PML (Muller and Dejean, 1999).
Nuclear inclusions of polyQ proteins have been shown to recruit transcriptional regulators. Specifically, CBP, the coactivator for CREB-mediated transcription, redistributes to huntingtin polyQ (Htt-N63-148Q) protein aggregates (Nucifora et al., 2001). Similarly, the tumor supressor p53 interacts with aggregates of a pathogenic amino-terminal region of huntingtin, httex1p (Steffan et al., 2000; Suhr et al., 2001). We therefore tested if CBP and p53 also relocate in response to GFP170* aggregates that do not contain polyQ tracks. CBP is diffusely distributed in the nucleus of control cells (Fig. 5A, arrowhead) but redistributes to the GFP170* nuclear aggregates in GFP170* transfected cells (arrows). The overall level of CBP seems to be increased in transfected cells, suggesting that transcription of CBP-responsive genes might be altered. The levels of p53 are significantly increased in cells containing GFP170* aggregates since p53 is barely visible in non-transfected cells (Fig. 5B). Like CBP, p53 is recruited to nuclear inclusions of GFP170* (Fig. 5B).
In addition to transcriptional factors, the mRNA splicing factor, SC-35, which normally localizes in the nucleus as nuclear speckles (Fu and Maniatis, 1990), relocates to nuclear inclusions formed by a truncated form of ataxin-3 (HA-Q78) (Chai et al., 2001). This response appears uncommon, and SC-35 is not recruited to aggregates formed by the full-length ataxin-3 (myc-ataxin-3-Q84) (Chai et al., 2001). We examined the relationship between nuclear aggregates of GFP170* and nuclear speckles. GFP170* aggregates do not significantly influence the distribution of nuclear speckles marked by SC35 (Fig. 5C).
The nuclear deposition of polyQ proteins has been linked to alterations in transcription (Okazawa, 2003). For example, a mutant form of huntingtin, httex1p, that sequesters p53 in inclusions, represses transcription of the p53-regulated proteins, p21WAF1/CIP1 (Steffan et al., 2000). Since p53 is also sequestered by nuclear GFP170* inclusions, we tested the effect of this sequestration on p53 transcriptional activity. The activity of p53 was analyzed by measuring transcription from a reporter construct composed of firefly luciferase fused to p21 promoter with two p53-responsive elements (p21-Luc) (Chinery et al., 1997). Analogous experiments were performed with Q80-GFP to allow direct comparisons. COS-7 cells were co-transfected with p21-Luc and either GFP170*, Q80-GFP, or a plasmid control. 48 h after transfection, luciferase activity in cell lysates was measured. COS-7 cells transfected with the control plasmid have wild type p53 activity, which is consistent with the results described previously (Ray et al., 1997). The luciferase activity in cells co-transfected with the GFP170* and the p21-Luc constructs is reduced to 30% of that in control cells co-transfected with control plasmid and the p21-Luc construct (Fig. 6A). This value is comparable to the luciferase activity in COS-7 cells expressing Q80-GFP, in which luciferase is reduced to 10% of control cells (Fig. 6A). These results indicate that p53 transcriptional activity can be repressed by GFP170* to a level similar to that caused by a polyQ protein.
Expression of polyQ proteins such as mutant huntingtin and atrophin-1 in cultured cells or in animal disease models leads to cellular toxicity (Nucifora et al., 2001). We therefore analyzed the effects of expressing GFP170* on cell cycle regulation and cell viability. We first examined the effects of GFP170* on DNA synthesis by measuring the incorporation of the thymidine analog 5-bromo-2′-deoxyuridine (BrdU) in COS-7 cells expressing GFP170*. BrdU incorporation during the S phase of mitosis is an indirect measure of cell proliferation. BrdU is incorporated in COS-7 cells expressing the GFP control plasmid (Fig. 6B). In contrast, BrdU is not incorporated in cells with GFP170* aggregates (Fig. 6B; arrows), suggesting a defect in DNA synthesis or a cell cycle arrest.
The effect of GFP170* expression was further analyzed by measuring the viability of cells expressing GFP170* by fluorescent-associated cell sorting (FACS) analysis. Control cells (mock-transfected) have a death rate of ~2.5%, probably due to transfection and experimental damage (Fig. 6C). In contrast, ~21% of cells containing GFP170* aggregates die 48 h after transfection. This number is similar to the ~17% of dead cells containing Q80-GFP aggregates 48 h after transfection. The percentage of dead cells expressing GFP170* or Q80-GFP is similar to that expressing the HD83Q huntingtin mutant (Waelter et al., 2001).
Neurodegenerative disorders, including HD, SBMA, DRPLA, and SCAs 1, 2, 3, 6, 7, and 17, are characterized by the formation of cytoplasmic and/or nuclear inclusions of polyQ proteins. The nuclear aggregates recruit molecular chaperones, ubiquitin, and proteasome proteins (Cummings et al., 1998; Davies et al., 1997; Paulson et al., 1997) and cause significant alterations in the nuclear matrix-associated structures containing PML (Skinner et al., 1997). In addition, transcription factors such as TAF (TATA-binding protein-associated factor), CREB (cAMP-responsive element-binding protein), and CBP (CREB-binding protein) are recruited to inclusions of polyQ proteins in vitro and in vivo (McCampbell et al., 2000; Nucifora et al., 2001; Shimohata et al., 2000). This recruitment influences transcriptional regulation (Dunah et al., 2002; Nucifora et al., 2001; Obrietan and Hoyt, 2004; Shimohata et al., 2000; Steffan et al., 2000; Suhr et al., 2001) and has been correlated with cytopathology. The cellular responses to the polyQ proteins appear directly linked to the polyQ tracks because the wild type proteins without extended polyQ tracks do not form nuclear inclusions and do not elicit cellular defects (Michalik and Van Broeckhoven, 2003; Orr, 2001; Ross, 2002). Some transcriptional factors, exemplified by CBP, contain poly-Q stretches, leading to the proposal that the recruitment and direct interactions with transcriptional regulators may be mediated through the polyQ tracks. It has not been addressed whether proteins lacking polyQ tracks can form nuclear inclusions, alter nuclear structure, and induce transcriptional responses analogous to those caused by polyQ proteins.
We report that nuclear aggregates are formed by GFP170*, a GFP-tagged fragment of the Golgi protein GCP170 that lacks a polyQ tract. Like aggregates of polyQ proteins, the nuclear aggregates of GFP170* recruit molecular chaperones and proteasomal components and cause redistribution of PML bodies (Davies et al., 1997; Ross, 2002; Schilling et al., 1999; Skinner et al., 1997; Waelter et al., 2001). It is therefore unlikely that the recruitment of these proteins is directly mediated through the polyQ tracks. Rather, it may involve mechanisms that recognize any misfolded protein as part of the cellular responses to either re-fold or clear aggregated proteins. PML bodies have been proposed to be depots (Maul et al., 2000; Negorev and Maul, 2001), and our findings confirm that they associate with aggregated proteins in the nucleus.
Like aggregates of polyQ proteins, the nuclear aggregates of GFP170* recruit CBP and p53 (McCampbell et al., 2000; Steffan et al., 2000; Suhr et al., 2001). The interaction between polyQ proteins and CBP requires polyQ repeats in both proteins since altering the polyQ domain in either protein prevents association (Nucifora et al., 2001). Whether polyQ proteins bind directly to CBP is unknown. Although polyQ-expanded huntingtin fragment can be co-immunoprecipitated with CBP from cell lysates, suggesting a direct interaction, the co-immunoprecipitation may reflect the recovery of a larger complex of proteins. The nature of GFP170* association with CBP remains to be defined. The interaction is unlikely to be direct since the Golgi-localized GCP170 has not been proposed to be involved in transcriptional regulation. It is more likely that binding of chaperones and proteasomes or sumoylation provides the link with CBP recruitment. The interaction of polyQ proteins with p53 may be direct or mediated through other cofactors (Steffan et al., 2000). The nature of GFP170* association with p53 remains to be characterized and may or may not involve mechanisms analogous to those recruiting CBP. Irrespective of the actual mechanisms, our results suggest that the cellular models used to examine the role of polyQ inclusions in pathogenesis reveal general (rather than polyQ-specific) cellular responses to the accumulation of misfolded protein in the nucleus.
Significantly, like the expression of polyQ proteins, the expression of GFP170* represses p53 transcriptional activity and causes cell death. The cell death pathways induced by GFP170* remain to be analyzed. Cell death may be due to the alteration of p53 activity since it has been documented that p53 may induce apoptosis through transcriptional repression (Oren, 2003). Some polyQ proteins appear to induce apoptosis (Ellerby et al., 1999; de Cristofaro et al., 2000; Saudou et al., 1998; Wanker, 2002). However, there are reports of non-apoptotic death caused by polyQ proteins (Moulder et al., 1999), indicating that the pathways leading to cell death are complex (Sawa et al., 2003).
Aggregation of non-polyQ proteins other than GFP170* has been linked to human diseases. For example, expression of mutant forms of aggregation-prone proteins such as superoxide dismutase (Bruijn et al., 1998; Durham et al., 1997), α-synuclein (Masliah et al., 2000), glial fibrillary acidic protein (GFAP) (Messing et al., 1998), or β-amyloid (Harper and Lansbury, 1997; Selkoe, 2003) in transgenic mice models of human disease results in the formation of large aggregates in selected neurons and neurodegeneration of the same neurons that mimic the pathology of FALS, Parkinson’s disease, Alexander’s disease or Alzheimer’s disease, respectively. However, in all known cases on non-polyQ proteins, the aggregates are either cytoplasmic or extracellular. To our knowledge, GFP170* represents the only non-polyQ protein that deposits in cytoplasmic as well as nuclear aggregates. Our findings indicate that nuclear aggregation may be caused by general features of misfolded proteins rather than the presence of polyQ tracks. Aggregate-induced nuclear alterations and transcriptional dysregulation may represent a general cellular response to the accumulation of any (polyQ or non-polyQ) aggregated protein in the nucleus. This suggests that the mechanisms for the neuropathology of polyQ neurodegenerative diseases may include common (polyQ-independent) events in addition to polyQ-specific responses.
We thank Dr. Susan Lyons for her help on preparing the primary neuronal cells from mice. We thank Dr. Xinbin Chen for the anti-p53 antibody and Ms. Kelly Harms in his laboratory for help on the luciferase assay. We thank Ms. Leigh Millican for technical support during the electron microscopy study. We thank Drs. Gail Johnson and Peter Detloff for helpful discussions. This work was partially supported by a NIH grant DK68074 (to L.F.).