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This article introduces a novel method to continuously monitor regional muscle blood flow by using Near Infrared Spectroscopy (NIRS). We demonstrate the feasibility of the new method in two ways: (1) by applying this new method of determining blood flow to experimental NIRS data during exercise and ischemia; and, (2) by simulating muscle oxygenation and blood flow values using these newly developed equations during recovery from exercise and ischemia.
Deoxy (Hb) and oxyhemoglobin (HbO2), located in the blood ofthe skeletal muscle, carry two internal relationships between blood flow and oxygen consumption. One is a mass transfer principle and the other describes a relationship between oxygen consumption and Hb kinetics in a two-compartment model. To monitor blood flow continuously, we transfer these two relationships into two equations and calculate the blood flow with the differential information of HbO2 and Hb. In addition, these equations are used to simulate the relationship between blood flow and reoxygenation kinetics after cuff ischemia and a light exercise. Nine healthy subjects volunteered for the cuff ischemia, light arm exercise and arm exercise with cuff ischemia for the experimental study.
Analysis of experimental data of both cuff ischemia and light exercise using the new equations show greater blood flow (four to six times more than resting values) during recovery, agreeing with previous findings. Further, the simulation and experimental studies of cuff ischemia and light exercise agree with each other.
We demonstrate the accuracy of this new method by showing that the blood flow obtained from the method agrees with previous data as well as with simulated data. We conclude that this novel continuous blood flow monitoring method can provide blood flow information non-invasively with NIRS.
Near Infrared Spectroscopy (NIRS) has been used to measure regional skeletal muscle deoxygenation [1-4] by presenting a balance between oxygen delivery and demand in muscle tissue. NIRS is beneficial since it is non-invasive and does not restrict ongoing activities at sports events such as skiing  and skating . Further, it is used to measure local muscular tissue circulation, namely blood flow  and oxygen consumption (O2) . The validity of these measurements and their relationship to global metabolism has been well established [9,10]. Thus, NIRS technology contributes to the understanding of regional muscular metabolism and O2 transport systems.
NIRS is a unique method since hemoglobin in smaller vessels can be detected with greater sensitivity than it can be in larger vessels when the probe is located on the surface of a muscle. Most of the hemoglobin information in NIRS comes from vessels less than 2 mm . This sensitivity feature of NIRS favors the measurement of the microvasculature of regional muscle. On the other hand, it is difficult to predict the hemoglobin distribution ratio between artery, capillary and vein. Nevertheless, NIRS measurements are well validated and are generally consistent with other methods [7-10,12]. Oxygen consumption and blood flow of regional muscles from NIRS (venous and arterial occlusion methods) have been compared to more global methods, large arterial flow using Doppler ultrasound method and global tissue flow using plethysmography, and differences have been found. For example, NIR-determined regional muscle blood flow change more with exercise than with global blood flow, while other global methods show a greater initial value and change less than with the NIRS method. This can be understood since measurements from global methods are averaged from other tissues, such as bones and skin or other neighboring muscles. It is clear that the NIRS method measures more regional blood flow from the microvasculature of a muscle than those methods using large arteries and global tissues. In addition, since NIRS targets red cell flow in the microvasculature and those are directly related to oxygen availability, NIRS based blood flow is a more important and closer parameter for O2 transport than large arterial blood flow. Therefore it is beneficial to develop an accurate method of regional blood flow measurement using NIRS. However current NIRS blood flow measurement by a venous occlusion maneuver, results in intermittent measurements and it is not always desirable to do occlusion every 10 seconds. Other technologies used for monitoring blood flow – Doppler ultrasound , MRI , and PET  – can also be troublesome since they require many restrictions on muscle performance and are not always available. Therefore, it is desirable to find a way to measure blood flow during muscle exercise without disturbing muscle performance during sports events.
In this investigation, we introduce a novel method that measures blood flow using information of tissue blood hemoglobin available with NIRS. The blood flow determination is achieved by calculating blood flow from the following two equations using NIR data on deoxy-hemoglobin (Hb) and oxy-hemoglobin (HbO2) : 1) a mass transfer equation of [HbO2] and 2) an equation that describes the rate of appearance of Hb as exactly analogous to the oxygen consumption rate, O2, with a two-compartment model of hemoglobin distribution. Similar equations are derived to describe 1) isometric exercises at the onset of oxygenation as an indication of O2 using a three-compartment model ; and 2) inflow and outflow hemodynamics due to the effects of external compression on the human breast with a two-compartment model . In addition, since these two equations describe the relationships among blood flow, oxygen consumption and muscle tissue oxygenation, we also use them for a simulation tool.
Thus, in this study, we introduce equations to describe blood flow with Hb and HbO2 kinetics and O2 in a two-compartment model. Then, we apply measured concentrations of Hb and HbO2 ([Hb] and [HbO2]) in arm muscles from experimental NIRS data to calculate blood inflow/outflow, observed in cuff ischemia and light exercise. Further, using the equations, we simulate and describe relationships between blood flow and reoxygenation kinetics during arm cuff ischemia and exercise. Finally, we compare calculated blood flow using measured differential information of [Hb] and [HbO2] to simulated cases, as well as to previous findings, and find good agreement among them. Thus, we demonstrate feasibility of the novel method to monitor regional muscle blood flow continuously by NIRS.
The principle of blood flow measurement is based on differential information of [Hb] and [HbO2] obtained by the NIRS. The NIRS data can be obtained by placing the NIRS probe on a muscle of interest and conducting an experiment, such as cuff ischemia and light exercise, that causes changes in the muscle [Hb] and [HbO2]. The blood flow calculations are based on a two-compartment model, where all the blood in the tissue belongs to either an arterial or venous compartment including capillaries. The first equation describes mass conservation in the two compartments of the muscle.
d[HbO2]/dt = Qa * Sa O2 - Qv * Sv O2 - O2 (1)
[HbO2] is a concentration of HbO2 (μM/kg tissue), Qa and Qv are arterial blood flow and venous blood flow respectively, expressed as hemoglobin mass as μMTHb/sec/kg tissue or μMTHb/sec/kg. O2 is the metabolic rate of the tissue, expressed as oxyhemoglobin carrying capacity μMHbO2/sec/kg tissue. Note that these two parameters, Q and O2, share the same units, namely hemoglobin concentration (total or oxyhemoglobin) per second in a kg tissue. This is similar to comparing O2 availability of Q and O2 utilization of O2, using the same unit of either ml O2/min/100 g or μM O2/min/100 g. (The translation of 1μM hemoglobin O2 carrying capacity is equivalent to 4 μM of O2, and 1 μM Hb O2/sec/kg of either Q and O2 translates to 24 μM O2/min/100 g, or 0.54 ml O2/min/100 g (O2) and 3 ml blood/min/100 g (Q).)
SvO2 is hemoglobin saturation with the oxygen in the venous blood:
Likewise SaO2 is hemoglobin saturation with the oxygen in the arterial blood, which is assumed to be 100%.
Equation 3 explains that oxygen is extracted from oxyhemoglobin, which is converted to deoxyhemoglobin with the same rate as O2 and some is lost through venous outflow.
d[Hb]/dt = O2 - Qv (1 - Sv O2) (3)
With the equations (1), (2), and (3), we come to the solutions of Qa and Qv as follows;
[HbO2]a represents HbO2 in the arterial blood, and [HbO2] represents total oxyhemoglobin ([HbO2] = [HbO2]a + [HbO2]v). Equations (4) and (5) demonstrate that we can calculate the blood flow of the tissue, inflow and outflow, separately with [HbO2], [Hb], and [HbO2]a, as well as the changes of [HbO2] and [Hb], and O2. The necessary normal resting values of muscle tissue [Hb] and [HbO2] in the equations can be measured by a frequency domain (FD) device (IQ system [18,19]) in arm finger flexor muscles. O2 information can be also measured by the slope of HbO2 disappearance during the cuff treatment .
The initial state of resting muscle is under steady state, therefore inflow is same as outflow (Qa = Qv). The inflow and outflow of the arm muscle, Qa and Qv correlate directly with THb, ([HbO2] + [Hb]) and changes in the following equation;
dTHb/dt = Qa - Qv (6)
Equation 6 states that the changes in THb are attributed to hyperemia, the imbalance between Qa and Qv that is typically seen in the recovery phase of either ischemia or exercise. Both situations occur due to the hypoxic status of the muscle tissue prior to the hyperemia . Oxygenation is defined by
Oxygenation = [HbO2] - [Hb] (7)
Oxygenation (μM/kg) is used as an indicator of relative oxygen saturation of the muscle tissue when the absolute values of saturations are not known , and can be negative value. Oxygenation values show how the simple expression of Oxygenation ([HbO2] - [Hb]) can represent relative values of saturation by comparison to StO2 and SvO2 in figures describing experimental data (Figures (Figures1,1, ,2,2, ,3)3) as well as simulation (Figures (Figures4,4, ,55).
StO2 = [HbO2]/THb (8)
The protocol was approved by the IRB of the University of Pennsylvania. Nine healthy volunteers aged from 21 to 30 years agreed to perform an arm cuff ischemia for three minutes with NIRS (IQ system, NIM incorporated, Philadelphia), which served as initial resting muscle parameters, as well as continuous time spectra of [HbO2] and [Hb]. Six of them also performed the cuff ischemia followed by a 90-second hand grip exercise with a continuous wave spectrometer (CWS) NIR device. Cuff ischemia was imposed for three minutes in the forearm at the pressure of 220 mmHg and a five minute recovery time period was observed. The light arm grip test was 90 seconds at the 25% MVC with a frequency of 0.5 Hz. The work intensities were determined by a dynamometer. We also did arm exercise at 75% MVC (0.5 Hz) with cuff in 3 subjects.
Frequency Domain NIRS was used to calculate absolute resting arm tissue concentrations of HbO2, Hb, StO2 and VO2 by arterial occlusion, prior to the ischemia, exercise and ischemic exercise protocols, according to absorption and scattering coefficients . The CWS was used during the protocols, and used a 3-wavelengths with light emitting diodes at 735, 805 and 850 nms, which in turn measured Hb and HbO2 concentration changes of the hand grip muscle tissues – Δ[Hb] and Δ[HbO2] .
The blood flow calculation from each individual needs resting values of [HbO2], [Hb] in each subject. To convert the Δ[Hb] and Δ[HbO2] obtained with CWS to the absolute concentration, [Hb] and [HbO2] in the cuff ischemia and exercise data, we add the values of the resting arm muscles measured by the IQ system to the CWS data. CWS data are smoothed with a 3rd order fitting (Matlab) and expressed as Δ[Hb] and Δ[HbO2]. Then the time course of CWS is analyzed for Qa and Qv. Another necessary parameter for Qa and Qv is O2, and in ischemia protocol, it is provided by the slope of HbO2, O2 is assumed to be unchanged throughout the measurement. However in the exercise protocol, O2 is assumed to have increased 5 times . The arterial and venous [HbO2] are also necessary for blood flow calculation and are calculated from the venous blood volume ratio of 28.3%/71.7%. This ratio comes from the venous blood saturation that is assumed to be near 49.1% .
For the simulation, we use mean values measured from the 9 healthy subjects with IQ systems, shown in Table Table1.1. The measured mean resting hemoglobin related values from the 9 healthy subjects are [Hb] and [HbO2] as 35.8 and 62.3 μM/kg, respectively, blood volume ([Hb] + [HbO2]) 98.1 μM/kg, and tissue saturation as 63.5%. The oxygen consumption of 0.185 μM HbO2/sec/kg is analogous to 0.1 ml O2/min/100 g, and is similar to published data . These are used as the initial resting values for the simulation. All the other necessary values used for the simulations are also listed in Table Table1,1, with the assumptions stated in the comments. We assume hemoglobin concentration of 12.8 g in 100 ml of blood, and that each gram of hemoglobin binds to 1.31 ml of O2, 0.3 ml of dissolved O2 in the 100 g of blood. The Qa, Qv values of 0.33 μM THb/sec/kg are analogous to 0.99 ml blood/min/100 g tissue, which is similar to published data .
In the exercise model, we assume the 25%MVC finger flexor exercise produced five times more O2 than resting value. At the end, the O2 recovered to the resting state within 20 seconds. Then we simulate 3 conditions of recovery blood flow; 1) same as resting, 2) 4 times, and 3) 10 times the resting blood flow. Matlab software is used to run the simulation algorithms and fitting procedures.
Typical results from a three minute arm cuff ischemia in healthy subjects are shown in Figure Figure1.1. The resting values of [THb], [HbO2], [Hb], are 128, 70.3, and 57.5 μM/kg respectively measured by the IQ system and StO2 is 55%, O2 is 0.2 μM HbO2/sec/kg. Average values of muscle hemoglobin in the 6 healthy subjects are listed in Table Table1.1. The healthy arm was cuffed at 220 mmHg, from 10 seconds to 190 seconds, and then, the cuff was released. Blood flow (Qa, Qv) became near zero during the cuff occlusion and abruptly increased immediately after the release of the cuff. The increase in the blood flow during the recovery phase is from 0.35 to over 1.1 μM THb/sec/kg, about three to four times greater than that of the resting blood flow (Figure (Figure1A).1A). The corresponding reoxygenation can be seen in HbO2 (Figure (Figure1B)1B) as well as saturation recovery profiles (Figure (Figure1C).1C). Interestingly, the arterial flow (Qa) kinetics is different from that of venous flow (Qv). BV increase during the recovery (hyperemia, Figure Figure1B,1B, black) resulting from the discrepancy of the kinetics between Qa and Qv (see Equation6). Overall half-time recovery of reoxygenation is approximately 25 seconds.
The high compensatory peak blood flow in the recovery from cuff ischemia for six healthy subjects is on average 4.2 ± 1.9 times of resting values and last for about two minutes and half recovery time of saturation is about 21.2 ± 4.5 seconds, on average. The reoxygenation kinetic phase form is similar to previous findings during arm cuff occlusion [25-27].
We tested the six healthy subjects with the 90 second handgrip test with NIRS. Figure Figure22 shows typical results of the blood flow of a subject during the handgrip test. The results showed that Qa, Qv are four to five times greater than the resting value during and at the end of the exercise lasting 15 to 30 more seconds (Figure (Figure2A2A as an example) in 6 subjects. The half recovery reoxygenation from the exercise is between 15 to 35 seconds (for example see Figure 2B,2C) in 6 subjects. This data agrees with previous studies [23,25,28,29].
We do not have to assume O2 to obtain Qa and Qv during the ischemic exercise protocol since we are able to calculate O2 by taking the initial slope of HbO2 during ischemic conditions from the NIRS spectra and inserting O2 into the equations provided (Equations 4 and 5). Therefore, the calculation of blood flow (Qa and Qv) is more quantitative (Figure (Figure3),3), than in the regular exercise cases, where in Figure Figure22 for example, we rely on an assumption of O2. Figure Figure33 shows an ischemic exercise of a healthy a subject. During 6 min. ischemia (time between 70 seconds and 430 seconds), after resting, handgrip exercise was imposed (between 180 seconds to 340 seconds). There is some flow during exercise even though the cuff pressure is 220 mmHg. The slight blood flow during the cuff ischemia can be confirmed by increases in the blood volume and HbO2 (Figure (Figure3B).3B). The greater blood volume increase is due to venous occlusion with some arterial flow leaking into the tissue. After the cuff ischemia, high blood flow is seen for 100 seconds and the peak blood flow increases as much as six times from the resting blood flow. The high blood flow in the recovery is consistent with studies of the similar protocols [23,25]. The reoxygenation rate is around 20 seconds.
The literature has demonstrated that the blood flow in the recovery from the ischemia is about four to eight times greater than the resting values [25-27]. Our simulation of cuff ischemia includes recovery blood flow values ranging from no compensation (resting value) to over-compensatory flow (ten times of resting value) in the recovery phase from the arm cuff ischemia. We simulate a situation where O2 and BV are maintained throughout the experiment for simplicity. Blood flow is completely stopped in the cuff ischemia and returned to parametric values in the recovery stage, from low (resting value, Figure Figure4A)4A) to middle and high blood flow (four and ten times as high as the resting values, Figure 4B,4C respectively). The duration of the middle and high blood flows in the recovery is 50 seconds and 23 seconds respectively in order to produce muscle reoxygenation to reach a little above the resting values. The results shown in Figure Figure44 indicate that the parametric blood flow in the recovery from the cuff ischemia alters the reoxygenation rate. The higher flow in the recovery caused the shorter recovery reoxygenation rates.
When the recovery blood flow from the cuff ischemia is the same as that of resting, the reoxygenation rate is extremely slow (half recovery time of approximately three minutes, Figure Figure4A).4A). On the other hand, the very high flow during the recovery phase (ten times as high as resting, Figure Figure4C)4C) results in a faster StO2, SvO2 reoxygenation rate and a half-recovery time of six seconds. In the middle of the two extreme cases, the blood flow is four times larger than the resting value (Figure (Figure4B)4B) and the recovery oxygenation rate is approximately 20 seconds for half-recovery time. This simulates the situation of a healthy sedentary arm, where the blood flow function is adequate.
We simulate the muscle deoxygenation and recovery of Hb and HbO2 profiles during 90 seconds handgrip exercise at 25% MVC. Van Beekvelt et al showed that the handgrip exercise test with 25% MVC required approximately five times of resting O2 and four times of resting blood flow . They also show some post exercise O2 and we assume similar trends during the first part of recovery (O2 decay takes 20 seconds in the recovery). We input these values of O2 and blood flow during the exercise and recovery phase in a simulation algorithm and treat the blood flow during the recovery as a variable parameter. The recovery blood flow range from the same as resting value (Figure (Figure5A)5A) to 4 four times (Figure (Figure5B)5B) and ten times (Figure (Figure5C)5C) of resting value, and we observe the recovery oxygenation kinetics of the arm muscle. For simplicity, we do not show any blood volume changes in the simulation (Qa = Qv). The sustaining recovery middle and high blood flow periods require for 26 and 13 seconds after the exercise respectively in order for the reoxygenation to reach beyond the resting values. The half time of reoxygenation in the middle to high blood flow become 20 seconds and 5 seconds respectively. The reoxygenation half time of 20 seconds are similar to the previous data when blood flow was 4 times of the resting . There is a delayed onset of the reoxygenation; 10, 5 and a few seconds of delay, observed in the exercise simulation, under 1, 4 and 10 times of resting blood flow cases (Figure 5A, 5B, 5C respectively). The delayed onset of reoxygenation is not seen in the ischemic cases.
We introduce a novel method to continuously measure arterial blood flow (Qa) and venous flow (Qv) through the NIRS signal of Hb and HbO2. We then demonstrate feasibility of use of the new method during muscle ischemia and exercise performance. This method does not require additional devices or maneuvers since information provided by NIRS is sufficient to calculate blood flow. Therefore, this is a novel, non-invasive and continuous method of blood flow that can be used in many settings of muscle sports exercise as well as in clinical application in real time. This method adds to the importance of NIRS since it provides oxygen concentration information as well as blood flow and oxidative metabolic rate in the muscles. This information will provide major physiological parameters and contribute to better understanding of the skeletal muscle metabolism and circulation status in healthy and diseased muscles.
In addition to the advantages of this method discussed above, we can use the described equations as a simulation tool to explain cases of high or low deoxygenation during perturbations and/or recovery of reoxygenation. This is very important in the diagnosis of vascular disease and myopathy [3,27,31-33], as well as when studying different types of muscle fibers and metabolic processes involved . Examples of simulation that can be used to interpret the reoxygenation kinetic in the ischemia studies, as well as recovery from muscle exercise protocols, are demonstrated. In both ischemia and a light exercise models, it is clear that recovery from deoxygenated muscle needs greater blood flow than resting flow, regardless of prior muscle conditions, because resting blood flow is only sufficient to maintain steady state of the same level of oxygenation. Then, we investigate the relationship between blood flow and half-time reoxygenation in recovery from ischemia and the light exercise. Various blood flows in recovery are simulated and summarized in Figure Figure6.6. The results show that both the ischemia and the light exercise models have reciprocal relationships between blood flow and reoxygenation, showing that reoxygenation is generally a good indicator of increased blood flow in those models. Note that the differences between the two models are primarily caused by the small oxygen debt treated in the light exercise case, where we input the O2 debt to be 10% of the total O2 expenditure during the light exercise occurred for 20 seconds. In the exercise model, there is a delayed onset of reoxygenation and the slower reoxygenation half time in the recovery than that of ischemia model (Figure (Figure6).6). This phenomenon has been seen in many previous studies , and greater oxygen debt relative to their blood flow might be the case to explain the slower reoxygenation. This issue should be more carefully investigated in future, but is beyond the scope of this feasibility investigation.
Another feature of the new method is that both arterial and venous blood flow (Qa and Qv), can be obtained separately. The NIRS is very sensitive to the differences of Qa and Qv by the blood volume differential information, therefore, it is necessary to separate these inflow and outflow measurements in order to obtain accurate blood flow measurements. To our knowledge, this method is the first to give both Qa and Qv separately from muscle regional blood flow measurements. Therefore, there has been no previous publication to compare both Qa and Qv with our data. However, the results from our data generally agree with previous studies [23,25-28].
A two-compartment model of hemoglobin distribution is necessary to get measurements of blood flow, Qa and Qv. However, hemoglobin distributes arteries, capillaries and venous vessels, and oxygen concentration changes constantly from small arterioles to the end of NIRS visible veins. The two-compartment model of hemoglobin location determines only two average values of [HbO2] and [Hb]. The arterial compartment is sequestered in the equation, and any involvement of changes in saturation or blood volume is interpreted as occurring in the venous compartment. If the biological system can not hold the two-compartment model, then the blood flow measurement may result in some error. For example, the arterial compartment may increase its capacity and will then reflect arterializations of the venous compartment. However, one may consider that the arteriole can transport oxygen and can, therefore, be treated as venous in our two-compartment model. But, our definition of the "venous compartment" is a compartment, where the existing blood volume changes its contents by diffusion or convection, therefore this includes arteriole that are capable of exchanging oxygen. Further, we treat this venous volume in the venous compartment as one mixed blood volume with an average value of Hb, and HbO2, even though we know there must be decreasing saturation along the capillary length from arterial side to venous side [34,35]. In spite of the oversimplification of the biological system, the agreements of our results to others may come from the fact that the NIRS is much more sensitive to smaller vessels than to larger ones . The difference between arterial and venous compartment here is not anatomical, but the functionality of the vessels, where the artery is only carrying saturated blood to the venous compartment, while the venous system can transport oxygen by diffusion and convection.
NIRS measures the microvasculature rather than the anatomical distribution of the vessels  which makes this blood flow measurement more useful in regional muscles. Therefore, we expect the outcomes of the blood flow calculations may differ from conventional techniques. Hemoglobin is used as a probe to measure the blood flow, therefore any discrepancy concerning red cell flow versus plasma flow and red cell flow in the capillary versus large vessel flow will result in a difference in blood flow values. Van Beekvelt noted that the NIRS gave a lower blood flow measurement than the plethysmograph. Using NIRS information to measure lower blood flow outcomes as opposed to measuring larger vessels creates a few concerns. First, the red cell flows five times slower in the capillary  than in larger vessels. Second, the hematocrit is 20% less than that in the blood of larger vessels . Third, the red cells cross capillaries intermittently while plasma flow is continuous . Finally, since NIRS visible vessels are smaller ones, its low hematocrit and lower flow rate result in a blood flow calculation smaller than total blood flow .
For the purposes of the blood flow measurement, this paper does not differentiate between myoglobin (Mb) and Hb. However, myoglobin is present in muscles. The molar contribution of the Mb in the NIRS is estimated at not more than 50% and the optical equivalency is 20%  which comprises a large contribution in the NIRS. Our total hemoglobin concentration in the arm muscles is 98.1 μM/kg. Assuming 12.8 g/100 ml of whole blood (12.8*10/64 mM) and comparing to the whole blood hemoglobin concentration, we can calculate blood concentration in the arm muscles to be approximately 5%. This is reasonably close to the tissue blood concentration; therefore we do not expect a high Mb contribution. With the fact that total arm hemoglobin is underestimated by the partial volume problem, the underneath tissue of the NIRS probe, namely fat and skin will contribute to the lower estimated blood concentration, there is as much as 20% of Mb in the muscles. Mb is ignored in the above equations by considering oxygen capacity in the blood and myocytes as one compartment. In addition, oxygen saturation in the Mb is treated as Hb in the capillary. Therefore, the blood flow may have been overestimated as the Mb contribution was taken into account when the muscle becomes very hypoxic and Mb becomes desaturated. In light exercise including less than 3 minutes cuff ischemia, we did not have significant desaturation of Mb . Since Hb and Mb can parallel each other [41,42] and the oxygen gradient, the timing of the changes in blood flow may cause overestimation of the blood flow in response to low tissue saturation when the Mb desaturation occurs.
Taking into account of these differences, this measurement method can be used to describe blood flow in a low flow situation. Figure Figure77 describes a case where a very small blood flow can be detected that may be hard to measure accurately by other modalities. In Figure Figure7,7, incomplete cuff ischemia was imposed in an arm at 160 mmHg, and gradual increases in Qa and Qv are observed under the cuff, and hemoglobin saturation becomes steady state at 5 min of cuff ischemia. Even though the blood flow is very slow, we can observe increasing blood flow, showing the physiological adaptation of the arm circulation and the sensitivity and strength of this technique of measuring the blood flow.
Figure Figure77 also demonstrates that the Qa and Qv are not always simultaneous – actual drainage of venous blood occurred at the beginning of the cuff, and at the end, arterial blood into the muscle and venous outflow is delayed until the muscle is adequately filled with the blood. This indicates the possibility that detected inflow and outflow can be used to better understand physiological functions during exercise tests.
The most common local muscle blood flow measurement method is the ultrasound Doppler blood flow . It is used on larger muscle groups since it obtains information from larger diameter vessels such as the brachial artery  and the femoral artery and produces volume per second quantity from the velocity. The method provides information on oxygen transport of entire muscle groups or complete exercise effects [43-45]. On the other hand, the NIRS techniques provide more regional muscle blood flow using a venous occlusion technique , and Hb, HbO2 from NIRS data. However the venous or arterial occlusion techniques are not a continuous measurement and can disturb the exercise performance. This new method presented in this paper focuses on regional blood flow and provides a continuous measurement as well as in real time. Moreover, it overcomes challenging situations when additional equipment and manipulation are not possible. This technique adds to the importance of NIRS since data may be interpreted by examining the relationship between tissue oxygenation changes and blood flow. This is important for diagnostic purposes and the interpretation of muscle performance and physiology.
The equations discussed above prove to be useful in the calculation of blood inflow and outflow. Since O2 must be known in order to analyze blood flow data, accuracy of the blood flow measurement is dependent upon O2 information. If O2 is not measured, then assumptions can be made from previous literature, as we did in the 25% handgrip exercise case (Figure (Figure2).2). If the assumption of O2 is close enough, then the blood flow values are correct. Since we do not know how different these O2 are between subjects, the accuracy can not be examined in this paper. When we seek higher accuracy, we must design experiments where we can measure O2 either simultaneously or at another time in the same subject. Simultaneous O2 measurement using an ischemia protocol is shown in Figure Figure11 and and3.3. Another assumption required, is SvO2. This is important to provide [HbO2]a and [HbO2]v. We used arterial venous blood ratio of 28.3%/71.7%, calculated from StO2 (our measurement) and SvO2  to calculate [HbO2]v and [HbO2]a in this paper. However, this assumption is only reliable in healthy subjects since we do not know if diseased persons or elite athletes have different values. SvO2 measurement from venous blood sampling may be a good solution, however the venous blood sampling be contaminated from other tissues, such as anastomosis and skin and bone. The majority of venous blood from the muscles consists of a mixture of blood from the capillary and shunting vessels, therefore its' saturation is higher than that of capillary outflow. In this case, it may be more reliable to use SvO2 from non-invasive venous occlusion technique [46,47] in order to measure more accurate mirovascular targeted blood flow.
Another issue concerns resting [Hb], [HbO2] which are required in the calculation. The absolute amount of [Hb], [HbO2] can be measured by TRS, Frequency Domain spectroscopy, as well as CWS with multi detection or multi wavelength methods. However resting [Hb] and [HbO2] in the human muscles have been measured by only a few researchers (, this paper). It is possible to use assumed values (Table (Table1)1) in the healthy individuals. However we will have to validate blood flow values.
We introduce a novel blood flow measurement method for non-invasive and continuous regional blood flow monitoring through the NIRS signal. This method uses differential information of muscle Hb and HbO2 and can be monitored in real time. We demonstrate feasibility of the method by showing measured blood flow time course during ischemia and light exercise on arm muscle, and by simulating these two models using the equations upon which the method is based. These blood flow time profiles agree with previous data as well as each other. Thus we conclude that this method is available to use for muscle activity studies in future.
We thank Guoqiang Yu, Gwen Lech for technical assistance. Grant acknowledgement; supported by NIH grant, HL44125