PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of plosbiolPLoS BiologySubmit to PLoSGet E-mail AlertsContact UsPublic Library of Science (PLoS)View this Article
 
From:
Published online Aug 1, 2006. doi: 10.1371/journal.pbio.0040267

Figure 3

An external file that holds a picture, illustration, etc.
Object name is pbio.0040267.g003.jpg
MS/MS of the Lid Subcomplex

(A) Spectrum showing the dissociation of the 34+ charge state.

(B) Expansion of the spectrum over the m/z range of 1,600–2,800. Three individual subunits are assigned Rpn5, Rpn6, and Rpn9 (orange, green, and blue circles, respectively).

(C) Expansion of the 8,000–16,000 m/z region. Three different hetrotrimers are assigned: Rpn5:Rpn6:Rpn8, Rpn5:Rpn8:Rpn9, and Rpn6:Rpn8:Rpn9 (blue, green, and orange stars, respectively). By extrapolation we can conclude that the subcomplex is composed of four subunits, Rpn5, Rpn6, Rpn8, and Rpn9. Rpn8 is not observed in its monomeric form; however, because it exists in all three different hetrotrimers, we can conclude it adopts a central position within the subcomplex.

(D) A schematic summarizes the structural data obtained from MS and MS/MS results. Red indicates core subunits, while purple and to a greater extent blue specifies peripheral subunits.

Images in this article

  • Figure 1
  • Figure 2
  • Figure 3
  • Figure 4
  • Figure 5
  • Figure 6
Click on the image to see a larger version.