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We have previously reported that actin filaments are involved in protein transport from the Golgi complex to the endoplasmic reticulum. Herein, we examined whether myosin motors or actin comets mediate this transport. To address this issue we have used, on one hand, a combination of specific inhibitors such as 2,3-butanedione monoxime (BDM) and 1-[5-isoquinoline sulfonyl]-2-methyl piperazine (ML7), which inhibit myosin and the phosphorylation of myosin II by the myosin light chain kinase, respectively; and a mutant of the nonmuscle myosin II regulatory light chain, which cannot be phosphorylated (MRLC2AA). On the other hand, actin comet tails were induced by the overexpression of phosphatidylinositol phosphate 5-kinase. Cells treated with BDM/ML7 or those that express the MRLC2AA mutant revealed a significant reduction in the brefeldin A (BFA)-induced fusion of Golgi enzymes with the endoplasmic reticulum (ER). This delay was not caused by an alteration in the formation of the BFA-induced tubules from the Golgi complex. In addition, the Shiga toxin fragment B transport from the Golgi complex to the ER was also altered. This impairment in the retrograde protein transport was not due to depletion of intracellular calcium stores or to the activation of Rho kinase. Neither the reassembly of the Golgi complex after BFA removal nor VSV-G transport from ER to the Golgi was altered in cells treated with BDM/ML7 or expressing MRLC2AA. Finally, transport carriers containing Shiga toxin did not move into the cytosol at the tips of comet tails of polymerizing actin. Collectively, the results indicate that 1) myosin motors move to transport carriers from the Golgi complex to the ER along actin filaments; 2) nonmuscle myosin II mediates in this process; and 3) actin comets are not involved in retrograde transport.
Actin filaments are crucial for cell migration and the maintenance of cellular morphology. In addition, a role for actin in membrane trafficking is emerging for both the endocytic and the secretory pathway (for recent reviews, see DePina and Langford, 1999 ; De Matteis and Morrow, 2000 ; Qualmann et al., 2000 ; Apodaca, 2001 ; May and Machesky, 2001 ; Stamnes, 2002 ). Although the role of actin in the secretory pathway has long been elusive, a breakthrough in our understanding has come with the use of highly specific inhibitors of actin polymerization, such as latrunculins and botulinum C2 toxin. Thus, actin filaments play a significant role in basolateral and Golgi-to-endoplasmic reticulum (ER) protein transport in polarized and nonpolarized mammalian cells, respectively (Müsch et al., 1997 , 2001 ; Valderrama et al., 2000 , 2001 ). Additional results that substantiated the involvement of actin in membrane trafficking in mammalian cells stem from the presence of members of myosin superfamily in organelle and vesicle transport in the secretory and endocytic pathways. For example, myosin V transports ER-derived vesicles in nerve cells (Tabb et al., 1998 ) and interacts with synaptic vesicle proteins (Prekeris and Terrian, 1997 ; Miller and Sheetz, 2000 ); myosin I was initially detected in isolated Golgi fractions and vesicles (Fath and Burgess, 1993 ; Fath et al., 1994 ; Montes de Oca et al., 1997 ), although it seems to be functionally and structurally involved in the endocytic pathway (Cordonnier et al., 2001 , and references therein); myosin VI has been localized in the Golgi complex (Buss et al., 1998 ) but to date has only been shown to be functionally involved in clathrin-mediated endocytosis (Buss et al., 2001 ); and finally, myosin II has been implicated in the formation of and immunolocalized in trans-Golgi–derived transport carriers (Narula and Stow, 1995 ; Ikonen et al., 1996 , 1997 ; Ecay et al., 1997 ; Müsch et al., 1997 ; Heimann et al., 1999 ), although with controversial results (Simon et al., 1998 ; Stow et al., 1998 ). Taken together, these results suggest that myosins are involved in intracellular trafficking by binding to subcellular compartments and generating force, either in the formation or the movement of vesicular carriers.
Until recently, the only known mechanism by which membrane structures moved along actin tracks was the myosin motors. However, the molecular mechanisms for the intracellular movement of certain pathogens through the formation of actin comets (for recent reviews, see Cossart, 2000 ; Goldberg, 2001 ) have also been observed in secretory and endocytic vesicles (Merrifield et al., 1999 , 2001 ; Rozelle et al., 2000 ; Taunton et al., 2000 ; Orth et al., 2002 ; Benesch et al., 2002 ; Lee and De Camilli, 2002 ). An actin comet is a characteristic structure easily visible by light microscopy, which results from a focalized actin polymerization (the tail) onto endomembranes and that acts as a driving force to propel them through the cytoplasm (for review, see Taunton, 2001 ). Vesicle rocketing basically requires N-WASP and Arp2/3 (Rozelle et al., 2000 ; Benesch et al., 2002 ). We have recently reported that N-WASP/Arp2/3 regulates Golgi-to-ER protein transport (Luna et al., 2002 ), and, therefore, it is possible that actin comets propel Golgi-to-ER transport carriers (for review, see Ridley, 2001 ).
Herein, we examine whether myosin motors, which exert force against actin filaments, move these Golgi-to-ER transport carriers or, in contrast, they are propelled by actin comets, as occurs with some pathogens. The results indicate that only myosin motors move to transport carriers along actin filaments in the Golgi-to-ER pathway. In addition, we provide direct evidence that nonmuscle myosin II is involved in this process.
Cy3-tagged native Shiga toxin fragment B was obtained from Ludger Johannes and Bruno Goud (Institute Curie, Paris, France; Johannes et al., 1997 ). The plasmid encoding myc-tagged mouse PIP5-KIα (PIP5K) was from Laura M. Machesky (University of Birmingham, Birmingham, United Kingdom; Rozelle et al., 2000 ), and the plasmids encoding myc-tagged MRLC2 wild-type (MRLCwt) and nonphosphorylated (MRLC2AA) forms were generated by site-directed mutagenesis by using polymerase chain reaction as described previously (Iwasaki et al., 2001 ). Antibodies against the myc-epitope were from 9E10 hybridoma, and those to Golgi-resident proteins giantin, mannosidase II, and galactosyltransferase were supplied by H.-P. Hauri (Biozentrum, Basel, Switzerland), K. Moremen (University of Georgia, Athens, GA), and E. Berger (University of Zürich, Zürich, Switzerland), respectively. DMEM and fetal calf serum (FCS) were from Invitrogen (Paisley, UK); secondary Alexa-488 or Alexa-546 F(ab′)2 fragments, cascade blue dextran, tetramethylrhodamine B isothiocyanate (TRITC)-dextran (10,000 mol. wt.), Fluo-4/acetoxymethyl ester (AM), and Pluronic F-127 were from Molecular Probes (Leiden, The Netherlands); 2,3-butanedione monoxime (BDM) was purchased from Sigma-Aldrich (St. Louis, MO); and Mowiol, thapsigargin, 1-[5-isoquinoline sulfonyl]-2-methyl piperazine (ML7), H7, and H89 were from Calbiochem (Nottingham, United Kingdom). Of note, BDM was reconstituted in DMEM (concentrated stock of 250 mM), H7 (10 mM) in distilled water, and ML7 and H89 (10 mM each) in a mixed solution of distilled water and ethanol (1:1). Unless otherwise stated, all other chemicals were from Sigma-Aldrich.
HeLa and normal rat kidney (NRK) cells were cultured in DMEM containing 10% FCS supplemented with 10 mM l-glutamine, penicillin (100 U/ml), and streptomycin (100 μg/ml). Cells were grown in a humidified incubator at 37°C and 5% CO2.
For microinjection, HeLa and NRK cells were grown for 1–2 d on normal glass coverslips and cultured in DMEM plus 10% FCS containing 25 mM HEPES, and supplemented with penicillin, streptomycin, and glutamine. Myc-tagged PIP5K cDNA constructs were first diluted to 50 ng/ml and then microinjected into the cell nucleus with an automated microinjection system (Carl Zeiss, Jena, Germany). After microinjection, the coverslips were transferred to a Petri dish containing fresh culture medium and returned to the incubator.
HeLa cells were transfected using Superfect reagent (QIAGEN, Valencia, CA) following the protocol indicated by the manufacturer. Briefly, cells plated on p35 culture Petri dishes were incubated for 2 h with MRLC2wt or MRLC2AA cDNAs (2 μg) previously mixed with Superfect in DMEM containing 10% of FCS. Subsequently, cells were washed and cultured in DMEM for 16–24 h.
NRK cells were plated onto glass-bottom dishes (Delta T culture dishes; Bioptechs, Butler, PA). After 1 d of culture, cells were rinsed three times with loading buffer (Hanks balanced salt solution with 10 mM d-glucose, 1.3 mM CaCl2, and 1.1 mM MgCl2) and loaded with Fluo-4/AM (4 μM) dissolved in loading buffer containing 0.4% Pluronic for 15 min at 37°C in a 5% CO2 atmosphere. Cells were then washed in loading buffer and incubated in DMEM for 30 min at room temperature to allow complete deesterification of intracellular AM ester. All calcium measurement experiments were carried out at 37°C (Delta T; Bioptechs) adapted to an inverted TCS 4D confocal microscope (Leica Microsystems, Heidelberg, Germany). Images were taken with a Plan Apochromatic 40×/1.3 oil objective by using the Ion Domain Quantify software from Leica Microsystems. Regions of Interest (ROIs) were set for each cell. Background-subtracted, fluorescence signals of each ROI were corrected for the bleaching of the Fluo-4 fluorescence signal and the mean fluorescence intensity of each ROI was calculated. Changes in the intracellular calcium concentration ([Ca2+]i) are given as the relative change in the fluorescence ratio F/F0 of Fluo-4/AM, where F is the fluorescence intensity at any time, and F0 is the baseline fluorescence intensity, as described previously (Bootman et al., 1997 ; Heemskerk et al., 2001 ). Values are expressed as the mean ± S.E. of the normalized fluorescence (F/F0). Data were obtained from 8 to 30 cells.
Infection with the temperature-sensitive (ts) mutant ts045 VSV was performed as described previously (Valderrama et al., 1998 ). Indirect immunofluorescence transport of VSV-G from ER-to-Golgi complex was performed following Bonatti et al. (1989) .
For the Shiga toxin (ST-B-KDEL) transport experiments, HeLa cells were first incubated in binding medium (FCS-free DMEM) and treated with cy3-ST-B-KDEL-fragment for 30 min at 4°C, and the unbound toxin was then washed for 5 min in ice-cold phosphate-buffered saline. Thereafter, cells were incubated with DMEM at 20°C for 2 h to accumulate the internalized ST-B-KDEL in early/recycling endosomes. They were then transferred to 37°C to synchronize the ST-B-KDEL transport to the ER via the Golgi complex.
Indirect immunofluorescence was carried out as described previously (Valderrama et al., 1998 , 2000 ) with the following antibody dilutions: anti-β-coatomer protein (COP), 1:60; anti-mannosidase II, 1:2000; anti-myc, 1:100; anti-galactosiltransferase, 1:500; anti-giantin, 1:500; anti-VSV-G, 1:50, and Alexa 488- and 546-conjugated secondary antibodies, 1:500 and 1:1000, respectively. TRITC- or coumarin-phalloidin were used to stain F-actin, at a dilution of 1:1000 and 1:100, respectively. The coverslips were mounted on microscope slides using Mowiol. Microscopy and imaging were performed with a BX60 epifluorescence microscope with a chilled DP-50 charge-coupled device camera (Olympus, Tokyo, Japan) or a TCS-NT confocal microscope (Leica Microsystems). The images were processed on PC computers using Adobe Photoshop 5.0.
Cells were washed twice in 100 mM cacodylate buffer (pH 7.2) and fixed with 2.5% glutaraldehyde in this buffer for 60 min at room temperature. Cells were then washed (3 × 5 min each) in cacodylate buffer and postfixed with 1% (vol/vol) OsO4/1.5% (wt/vol) K4Fe(CN)6 in 100 mM cacodylate buffer for 1 h at 4°C. Cells were scraped, pelleted, and treated for 1 h at 4°C with 1% tannic acid in cacodylate buffer, rinsed in distilled water, and stained en bloc with 1% aqueous uranyl acetate for 1 h, followed by dehydration through graded ethanol solutions and embedding in Epon 812. Ultrathin sections were stained with lead citrate for 2 min and observed in a 301 electron microscope (Philips, Eindhoven, The Netherlands). Randomly selected micrographs were taken at the same final magnification (47,500×) and analyzed using point-counting procedures. The Golgi complex (GC) was defined as a group of cisternae organized in stacks with tubular and vesicular structures. Total Golgi complex (tGC) was defined as an area containing at least one cisterna and peri-Golgi vesicles, with an arbitrary border in the cytoplasm surrounding the Golgi (Renau-Piqueras et al., 1987 ). Intermediate elements in continuity with the rough ER were excluded. The stereological parameters were determined using standard procedures (Renau-Piqueras et al., 1987 ). The minimum sample size (number of micrographs; Table Table1)1) of each stereological parameter was determined by the progressive mean technique (confidence limit of 5%). The results were expressed as means ± SD and compared using the Student's t test.
To determine whether actin-dependent motors govern membrane dynamics at the ER/Golgi interface, we first used BDM as a broad-spectrum inhibitor of both conventional and unconventional myosin Mg2+-ATPase activity (Higuchi and Takemori, 1989 ; Herrmann et al., 1992 ; Cramer and Mitchison, 1995 ; Lin et al., 1996 ). At fluorescence level, NRK cells treated with BDM at concentrations ranging from 5 to 20 mM showed no change in the Golgi complex morphology in comparison with untreated control cells (Figure (Figure1,1, A and C), but at higher concentrations (30 and 40 mM) the Golgi complex showed a more compact morphology (Figure (Figure1E).1E). Actin stress fibers were virtually unaltered when cells were treated with BDM from 5 to 20 mM (Figure (Figure1D),1D), but they diminished in number when it was used at 40 mM (Figure (Figure1F).1F).
Because myosin II, a major actin-based motor protein, has been implicated in the formation of transport vesicles at the trans-Golgi network (TGN) level (Müsch et al. 1997 ; Stow et al., 1998 ), we then examined the effect of ML7, a compound that inhibits myosin light chain kinase (MLCK) function, thus specifically preventing myosin II activation via phosphorylation (Saitoh et al., 1987 ). When NRK cells were treated with ML7 at concentrations of 15 and 30 μM, the Golgi complex was perinuclear but, instead of showing its continuous reticular shape characteristic of untreated NRK cells (Figure (Figure1A),1A), a pearl necklace-like morphology was observed (Figure (Figure1G).1G). Furthermore, ML7 also decreased actin stress fibers (Lamb et al., 1988 ) and produced numerous actin-stained cytoplasmic spots (Figure (Figure11H).
To determine the site and mode of action of BDM and ML7, an ultrastructural (Figure (Figure2)2) and stereological analysis (Table (Table1)1) of the Golgi complex was performed. BDM at 20 or 40 mM induced severe changes in the Golgi ultrastructure, the cisternae seeming swollen (Figure (Figure2B).2B). BDM increased the volume occupied by the tGC in the cytoplasm (Vv tGC/cyt; Table Table1).1). This could be attributable to an increase either in the volume of Golgi cisternae (Vv cist/tGC) or in the volume of peri-Golgi vesicular structures (Vv ves/tGC). At the electron microscopic (EM) level, the former is visualized as swollen cisternae (Figure (Figure2B)2B) and the latter results from the decrease in the Golgi membrane surface density (Sv cist/tGC). In cells treated with ML-7 (15 and 30 μM), similar results were obtained regardless of the concentration used, but the decrease in the Golgi membrane surface density (Sv cist/tGC) was greater. As expected, this was followed by a much higher volume density of peri-Golgi vesicular structures (Vv ves/tGC), which at the EM level are viewed as numerous vesicular structures of uniform size closely attached to Golgi cisternae (Figure (Figure2C).2C).
Because actin filaments facilitate retrograde transport from the Golgi complex to the endoplasmic reticulum (Valderrama et al., 2001 ), we next studied whether myosin activation is required in ER/Golgi membrane dynamics. We first examined whether these antimyosin agents (BDM and ML7) alter the disassembly of the Golgi complex induced by brefeldin A (BFA) (Figure (Figure3).3). NRK or HeLa cells were first treated with BDM and then with BFA for different times. BDM suppressed the disassembly of the Golgi complex induced by BFA (Figure (Figure3,3, E–H and Q). To examine the involvement of myosin II in this membrane route, we analyzed the effects of inhibiting MLCK by using ML7, and of overexpressing nonmuscle myosin II regulatory light chain (MRLC2) constructs. ML7 also produced a significant delay in the BFA-induced Golgi complex disassembly (Figure (Figure3,3, I-L and Q), but its inhibitory effect was weaker than that produced by BDM (Figure (Figure33 Q). In addition to its effect on MLCK, ML7 is also able to partially inhibit protein kinase (PK) A and PKC enzymatic activities, albeit with a much lower efficiency than for MLCK (Ki values of 21 μM, 42 μM, and 300 nM, respectively). To examine whether PKA or PKC were also significantly involved in the retrograde pathway, NRK cells were treated with H7 or H89 (30 μM). Neither H7- nor H89-treated cells altered the kinetics of the disassembly of the Golgi complex induced by BFA (see supplementary data). This suggests that neither PKA nor PKC is apparently involved in the Golgi-to-ER pathway.
To gain further insight into the involvement of nonmuscle myosin II, HeLa cells were transfected with either the recombinant myc-tagged MRLC2 wild-type (MRLC2wt) or with a MRLC2 mutant that mimics its unphosphorylated state, in which both Ser19 and Thr18 were substituted by Ala (MRLC2AA; Iwasaki et al., 2001 ). The kinetics of the BFA-induced Golgi disassembly was greatly slowed down in cells expressing MRLC2AA (Figure (Figure44 F, F′ to H, H′), whereas it remained unaltered in cells overexpressing the MRLC2wt form (Figure (Figure44 B, B′ to D, D′). As expected, MRLC2AA reduced the presence of actin stress fibers stained with coumarin-phalloidin (our unpublished data), and it was mostly diffuse in the cytoplasm (Figure (Figure4,4, E–H), whereas MRLC2wt was mostly on actin stress fibers (Figure (Figure4,4, A–D). Collectively, the results strongly indicate that nonmuscle myosin II is involved in the Golgi-to-ER pathway.
Next, we used Shiga toxin (ST-B) as a protein marker of the Golgi-to-ER pathway. Unlike NRK, HeLa cells bind Shiga toxin, which is transported to the ER via early endosomes and the Golgi complex (for review, see Sandvig and van Deurs, 2000 ). Cells were treated as reported previously and the toxin transport was imaged by confocal microscopy (Valderrama et al., 2001 ). Briefly, cells were incubated at 4°C with cy3-tagged fragment B of the toxin bearing the KDEL sequence (ST-B-KDEL; Johannes et al., 1997 ) and, after 30 min, incubated at 19.5°C for 1 h to accumulate the internalized toxin in the early/recycling endosomes. Subsequently, cells were treated with or without BDM (40 mM; our unpublished data) or ML7 (30 μM; Figure Figure6)6) and thereafter they were transferred to 37°C to synchronize the retrograde transport of Shiga toxin to the ER. In control cells (Figure (Figure5,5, A–H), ST-B-KDEL travels from early/recycling endosomes (Figure (Figure6A)6A) via the Golgi complex (Figure (Figure5,5, C–E) to the ER (Figure (Figure5G)5G) giving rise to the characteristic ER-like staining pattern as the toxin is retained. In contrast, in BDM- (our unpublished data) and in ML7-treated cells (Figure (Figure5,5, I–O), after 6 h of transport, ST-B-KDEL was still clearly located in the Golgi complex (Figure (Figure5O).5O).
We next studied putative changes in the ER-to-Golgi membrane movement after myosin inhibition. To this end, NRK or HeLa cells were first treated with BFA to induce the fusion of Golgi membrane with ER, then incubated with BDM/ML7, and subsequently BFA was withdrawn from the culture medium. The rebuilding of the Golgi complex was visualized by fluorescence microscopy by using anti-mannosidase II (Man II) antibodies. No differences were observed in the kinetics of the reassembly of the Golgi complex either in cells treated with BDM or ML7 or in those expressing the MRLC2AA mutant (our unpublished data). In addition, we assayed the ER-to-Golgi transport of the VSV-G protein by fluorescence microscopy with anti-VSV-G protein (Figure (Figure6).6). Briefly, after infection of cells with ts045 mutant VSV, the cells were kept at nonpermissive temperature (40°C), at which the VSV-G protein is synthesized but retained in the ER, and then treated with BDM or ML7. Cells were shifted to the permissive temperature (32°C) and the intracellular location of VSV-G protein was monitored by fluorescence microscopy with an anti-VSV-G monoclonal antibody. Neither BDM (Figure (Figure6)6) nor ML7 (our unpublished data) altered the transport of VSV-G protein from ER to the Golgi complex.
Taken together, the data strongly indicate that only myosins, and in particular nonmuscle myosin II, are involved in the Golgi-to-ER membrane pathway.
Treatment of cells with BFA leads to the formation of tubular extensions that have been implicated in the backflow of Golgi components to the ER. These tubules connect the two compartments and mediate the movement of Golgi lipids and proteins to the ER membranes where their membranes finally merge (Sciaky et al., 1997 ). Because myosin II has been reported to be involved in the formation of vesicular carriers from the TGN (Müsch et al., 1997 ; Stow et al., 1998 ), we analyzed whether the formation of tubules from the Golgi complex induced by BFA was impaired by BDM or ML7. However, the first morphological event that is visualized in cells treated with BFA is the release of COP components, such as β-COP from Golgi membranes, which is required for the redistribution of Golgi membranes into the ER (Donaldson et al., 1990 ; Scheel et al., 1997 ). Thus, it is conceivable that BDM and ML7 inhibit the BFA-mediated retrograde flow from the Golgi complex to the ER by inhibiting the release of coatomer from the Golgi complex. Therefore, we first carried out an immunofluorescence study using an anti-β-COP antibody on cells pretreated with BDM/ML7 or expressing MRLC2WT/MRLC2AA. BFA-induced release of β-COP from the Golgi complex was unaffected by BDM or ML7 (Figure (Figure7,7, G and K, respectively) or MRLC2WT/AA (our unpublished data). Regardless of whether cells were preincubated with BDM before BFA treatment or both agents were added simultaneously (see below), β-COP release was indistinguishable from that in cells treated with BFA alone (Figure (Figure7C).7C). β-COP was released at BFA incubation times, in which the Golgi complex remained morphologically unaffected (Figure (Figure7,7, H and L), and neither BDM nor ML7 (Figure (Figure7,7, E and I) nor MRLC2WT/AA (our unpublished data) affected the distribution of the coatomer component.
We next analyzed the kinetics of the appearance of tubules from the Golgi complex when BDM- or ML-7–treated cells were incubated with low concentrations of BFA (Figure (Figure8).8). After 4 min of BFA treatment, the tubules were seen in both control and pretreated cells with BDM and ML7 (Figure (Figure8,8, B, F, and J). After 6 min, the tubules were still visible in BDM/ML7-pretreated cells (Figure (Figure8,8, G and K), although the characteristic ER-like staining pattern was already observed in some control cells (our unpublished data). After 10 min, most of the BDM- or ML7 plus BFA-treated cells still showed Man II-containing tubules and a Golgi structure was still clearly recognizable (Figure (Figure8,8, H and L), whereas all the control BFA-treated cells showed the expected ER-like staining pattern for Man II (Figure (Figure8D).8D). The percentage of cells that showed Golgi-emerging tubules induced by BFA was unaltered by BDM or ML-7 treatments, but the tubules remained longer in the cytoplasm before their fusion with ER membranes (Figure (Figure8M).8M). Similarly, in MRLC2AA-transfected and BFA-treated cells, long Golgi-derived tubules were still visualized when their neighboring nontransfected cells already displayed the characteristic ER-like staining pattern (Figure (Figure4,4, G′ and H′).
Although BDM affects the actomyosin system and inhibits myosin ATPase in vitro (Higuchi and Takemori, 1989 ; Herrmann et al., 1992 ; Cramer and Mitchison, 1995 ) and in vivo (Cramer and Mitchison, 1995 ; Lin et al., 1996 ; Gloushankova et al., 1998 ; Steinberg and McIntosh, 1998 ), it has also been reported that BDM affects intracellular calcium concentration (Steinberg and McIntosh, 1998 , and references therein). This reported collateral effect of BDM on intracellular calcium levels (in particular when it is used at higher concentrations of 30 mM; Phillips and Altschuld, 1996 ) could be relevant because the sequestration of Ca2+ to intracellular stores is required for the anterograde and retrograde transport between the ER and the Golgi complex (Beckers and Balch, 1989 ; Ivessa et al., 1995 ; Chen et al., 2002 ). Thus, to test whether the observed effects on the disassembly of Golgi induced by BDM or ML7 were the result of alterations in calcium homeostasis, we monitored in vivo the cytoplasmic calcium release from intracellular stores. HeLa cells were loaded with Fluo 4/AM and the fluorescence ratio was continuously monitored under the confocal microscope (Figure (Figure9).9). Unlike ML7 (Figure (Figure8C),8C), BDM induced a small biphasic rise in [Ca2+]i (1.34 ± 0.02; Figure Figure9,9, A and B). To assess the relative contribution of BDM to the [Ca2+]i increase from the total Ca2+ from intracellular stores, cells were subsequently treated with thapsigargin, a selective inhibitor of the ER Ca2+-ATPase, a pump that maintains a high concentration of Ca2+ in the lumen of the ER (Thastrup et al., 1990 ). When thapsigargin was added 1.5 min after BDM, a much larger and monophasic rise in [Ca2+]i was registered (4.1 ± 0.11; Figure Figure9A).9A). When thapsigargin was added 30 min after BDM, a smaller peak was also observed but it lasted much longer (Figure (Figure9B).9B). After ML7 treatment, thapsigargin induced a small monophasic transient increase in [Ca2+]i (2.1 ± 0.06; Figure Figure9C)9C) regardless of the time interval used between the two compounds. Taking these results into account, we examined whether the effect of BDM on the disassembly of the Golgi complex induced by BFA was reproduced when cells were pretreated with BDM for only 1–2 min or when BDM and BFA were added simultaneously. In both experimental conditions, BDM suppressed the effect of BFA (our unpublished data). Thus, the inhibition of the BFA-induced Golgi disassembly produced by BDM and ML7 is not merely the result of an alteration in calcium homeostasis.
Activation of myosin II via phosphorylation of the light chain can be regulated not only by MLCK but also by Rho kinase, which, in turn, is activated by RhoA-GTP directly phosphorylating MLC at Ser 19 or via inactivation of myosin phosphatase (Amano et al., 1996 ; Kimura et al., 1996 ; Kureishi et al., 1997 ). To test whether Rho-dependent signaling, in addition to MLCK, plays a role in retrograde transport, we assayed BFA-induced Golgi complex disassembly in cells that had been pretreated with Y-27632, a selective inhibitor of Rho-kinase (Uehata et al., 1997 ), at concentrations of 25 and 50 μM. Under these conditions, Y-27632 induced the disappearance of actin stress fibers (our unpublished data), but the Golgi complex morphology and the kinetics of the disassembly of the Golgi complex induced by BFA remained unaltered (Figure (Figure3,3, M–Q). Thus, Rho-kinase does not play a role in myosin II activation during the Golgi-to-ER membrane flow and this activation is largely regulated by MLCK alone.
Activated Cdc42 regulates retrograde transport via N-WASP, which is recruited to the Golgi complex (Luna et al., 2002 ). This suggests that an actin-based propulsion of transport carriers from Golgi to the ER is possible. To explore this possibility, we overexpressed myc-tagged PIP5K isotype I in cells by microinjection of its cDNA into the nucleus of HeLa cells, producing the appearance of filamentous actin-containing comets as early as after 1 h of expression. To examine whether endocytic transport carriers were present at the heads of these actin comets, cells were first microinjected with PIP5K and, after 1–2 h of expression, they were incubated at 4°C with native Shiga toxin fragment B (ST-B). Subsequently, cells were washed and transferred to 37°C for short internalization times before chemical fixation (Figure (Figure10).10). Confocal images showed that ST-B was not present at the heads of actin comets (Figure (Figure10,10, A and A′). Next, we examined the membrane pathway from the Golgi complex to the ER. Cells were incubated with ST-B at 4°C for 30 min, washed, internalized at 37°C (at this temperature, ST-B shows a steady-state distribution at the Golgi complex, although it continuously and rapidly cycles through the ER), and finally PIP5K DNA was microinjected into the nucleus. Again, no transport carrier-containing ST-B colocalized with the head of actin comets (Figure (Figure10,10, B and B′). PIP5K expression times longer than 3 h were unsatisfactory because the Golgi complex seemed extensively fragmented (our unpublished data). Taken together, these results strongly suggest that PIP5K-induced actin comets do not propel ST-B–containing transport carriers.
We have recently reported that actin facilitates Golgi-to-ER protein transport. Actin is present in the lateral rims of Golgi cisternae and in Golgi-derived transport intermediates (Valderrama et al., 2000 , 2001 ). In the present study, we have examined the mechanism supporting this physiological role of actin in trafficking between the ER and the Golgi. Currently, there are two possibilities; this involves either the actin-based motors or actin comets. We have tested which of these two processes is physiologically relevant in cultured mammalian cells, assuming that simultaneous action of both mechanisms in the Golgi-to-ER protein transport is unlikely. We used pharmacological and molecular experimental approaches to inhibit myosin motor activity or to generate actin comets. Our results can be summarized as follows: only the retrograde Golgi-to-ER pathway is inhibited when myosin motors are impaired; this alteration is not caused by changes in calcium homeostasis; and ST-B is not observed in the actin comet head at any time during its transport from the plasma membrane to the ER via the Golgi complex. Collectively, our results indicate that myosin motors and not actin comets are mediators of the actin-based Golgi-to-ER protein transport.
Myosins have been implicated in vesicular transport (for review, see Allan and Schroer, 1999 ). In particular, using BDM and a monoclonal antibody (AD7), it was reported that myosin II is involved in the formation of transport intermediates at the TGN (Müsch et al., 1997 ). However, this result has been challenged by Simon et al. (1998) , mainly because of the specificity of this antibody (see Stow et al., 1998 for details). Additional experimental evidence that myosin II is the vesicle motor has been obtained by through use of ML7, a selective inhibitor of MLCK (Ruchhoeft and Harris, 1997 ). Inhibition of MLCK by ML7 blocks vesicle movement, presumably by inhibiting the activity of myosin II (for review, see DePina and Langford, 1999 ). Hence, ML7 is a good reagent to test the involvement of myosin II in the biological processes examined. Herein, we observed that impairment in the BFA-induced disassembly of the Golgi complex is much more severe when cells are pretreated with BDM than with ML7. BDM could produce the higher inhibitory effect because of the simultaneous inhibition of several myosins (see below). The results with ML7 confirm those observed with BDM, but with the additional advantage that ML7 produced no alterations in the levels of intracellular calcium and was also more restrictive in the molecular target (myosin II). In this regard, the unaltered kinetics in the Golgi disassembly induced by BFA in cells treated with H89 and H7 suggest that neither PKA nor PKC are apparently involved in the Golgi-to-ER pathway. This is in accordance with the findings of Lee and Linstedt (2001) , which despite the observation that the redistribution of Golgi residents to the ER was inhibited by H89 (at 50 μM), they discarded that PKA was the kinase required for the bidirectional transport at the ER/Golgi interface, because a number of other protein kinase inhibitors at concentrations reported to inhibit PKA and PKC (120 μM H8; 9 μM KT5720; 60 μM H7) inhibited neither the retrograde nor anterograde transport. Thus, the use of ML7 at concentrations that are widely used in vivo are indicative that myosin II is involved in the retrograde protein transport. This was confirmed using a mutant of the nonmuscle myosin II regulatory light chain (MRLC2AA) that cannot be phosphorylated. Cells transfected with this mutant showed similar perturbation in the Golgi disassembly induced by BFA treatment as that observed in ML7-treated cells (Figures (Figures44 and and3,3, respectively). However, myosin II is neither a resident Golgi protein (Narula et al., 1992 ; de Almeida et al., 1993 ) nor a processive motor. These properties constrain its mode of action at the Golgi, and for this reason it has been suggested that the myosin II tail participates only in the budding of transport carriers (Stow et al., 1998 ). Consequently, this assumption and the observation that the delay in the Golgi disassembly induced by BFA is much more severe in BDM- than in ML7-treated cells, suggest the participation of a second (processive) motor that subsequently helps move the newly generated transport carriers to their final destination (in this case the ER). In this respect, myosins V and VI are processive motors (which means that it takes successive steps along the actin filament) that are reported to be located in the Golgi complex and involved in vesicular trafficking in some cell types (Nascimento et al., 1997 ; Buss et al., 1998 ; DePina and Langford, 1999 ; Miller and Sheetz, 2000 ; Schott et al., 2002 ; da Silva Bizario et al., 2002 ) and, thus, they could be acting in tandem with myosin II at the Golgi complex. However, we cannot completely rule out the sole implication of myosin II, because despite that this motor does not take successive steps along the actin filament, multiple myosin II moieties could show successive steps along a filament (a processive movement). This can occur if a second myosin II motor binds the filament before the first motor releases (Howard, 2001 ).
The observation that the emergence of tubules from the Golgi induced by BFA remains unaltered in BDM/ML7-treated cells suggests that actin motors are not directly involved in the formation of these tubules. Interestingly, BFA-induced tubules remain longer in the cytoplasm before their fusion with the ER. In addition, EM data suggest that myosin II is not directly involved in budding, because in ML7- and, to a lesser extent, BDM-treated cells, there is a significant accumulation of peri-Golgi vesicles (Table (Table1;1; Figure Figure2C),2C), probably because they remain blocked in their movement to the ER once they have been formed at the lateral rims of the Golgi complex.
Taken together, these observations lead to the following three possibilities: 1) myosins are only involved in the locomotion process of transport carriers. Consequently, their inhibition delays the arrival of Golgi-derived transport carriers to putative ER entering sites; 2) transport carriers are normally transported via microtubules to the vicinity of these ER entering sites, but cannot be efficiently translocated to actin filaments, and their fusion to the ER is, therefore, delayed. Simultaneously, the BFA-induced tubules are continuously growing from the Golgi and become longer compared with those observed in control cells. This possibility suggests the presence of actin cytoskeleton and myosin motors associated with ER. The former has been reported in photoreceptor cells and in the corneal epithelium (Svoboda and Hay, 1987 ; Baumann and Lautenschlager, 1994 ); the latter is the case of myosin V, which is involved in the ER transport into the dendritic spines of neuronal cells (Tabb et al., 1998 ; Molyneaux et al., 2000 ). However, we are not inclined for this possibility because first, we have not observed a significant association of β/γ-actin isoforms with ER membranes at the ultrastructural level by using monospecific polyclonal antibodies (J.A. Martínez-Menárguez and G. Egea, unpublished data); second, ST-B-KDEL is retained in the Golgi complex of cells with disrupted myosin function; and third, transport carriers are captured by peri-Golgi actin filaments via myosin motors as soon as they are formed at the lateral rims of Golgi cisternae. Subsequently, transport carriers are translocated to the microtubules for their movement to the ER. The presence of actin isoforms in Golgi-derived buds and vesicles (Valderrama et al., 2000 ) and the accumulation of ST-B in the Golgi complex either when actin filaments are disrupted (Valderrama et al., 2001 ) or myosin function is inhibited (this study) strongly support this possibility.
Plasma membrane and endomembranes are able to recruit N-WASP-Arp2/3 that could trigger focalized actin polymerization (actin tail), which in turn acts as a driving force for the locomotion of transport carriers or organelles (Merrifield et al., 1999 , 2001 ; Rozelle et al., 2000 ; Taunton et al., 2000 ; Lee and De Camilli, 2002 ; Benesch et al., 2002 ; Orth et al., 2002 ). Most of studies that report actin comets associated with endo/exocytic transport vesicles use cells that overexpress PIP5K (Rozelle et al., 2000 ; Benesch et al., 2002 ; Lee and De Camilli, 2002 ; Orth et al., 2002 ). Using this same experimental approach, our results clearly show that transport carriers containing ST-B are not visualized at the head of actin tails during trafficking from plasma membrane to the ER (Figure (Figure10).10). Nonetheless, small and short-lived actin comets could be generated concomitantly to the formation of transport carriers at the lateral rims of the Golgi, facilitating their final scission or separation from the cisternae. However, this possibility seems unlikely because the retrograde transport of ST-B and Golgi enzymes remained unaltered in cells expressing a truncated form of N-WASP lacking the Arp2/3 binding site and, therefore, unable to form actin comets (Luna et al., 2002 ). These results, together with the previous observation that VSV-G is rarely associated with actin comets (Rozelle et al., 2000 ), strongly indicate that actin comets do not propel transport carriers at the ER/Golgi interface.
In conclusion, our findings indicate that retrograde transport carriers use actin-based motors, most likely myosin II in their trafficking from Golgi membranes to the ER.
We thank Laura Machesky for helpful discussions and PIP5-KIα cDNA, and Montse Vilella for critically reading of the manuscript. We are also grateful to Bruno Goud, Hans-Peter Hauri, Eric Berger, and Kelley Moremen for antibodies and reagents; Robin Rycroft for editorial assistance; Maite Muñoz for skillful technical assistance; and Serveis CientificoTècnics de la Universitat de Barcelona (Campus Casanova) for help with confocal microscope. The work in the laboratory of G.E. is supported by grants from Comisión Interministerial de Ciencia y Tecnologica (SAF2000-0042) and Comissió Interdepartamental de Recerca i Innovació Tecnològica (AGP2002). J.D., F.V., and M.T. are recipients of predoctoral fellowships from Fondo de Investigaciones Sanitarias, Universitat de Barcelona, and Ministerio de Educacion y Ciencia, respectively. G.E. dedicates this article to the memory of the innocent victims of terrorist attacks wherever they occur.
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Article published online ahead of print. Mol. Biol. Cell 10.1091/mbc.E02–04–0214. Article and publication date are at www.molbiolcell.org/cgi/doi/10.1091/mbc.E02–04–0214.