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Nucleic Acids Res. 1999 January 15; 27(2): 426–428.
PMCID: PMC148196

A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells.


We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector replicates episomally in CHO cells. It occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selection pressure.

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