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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Clin Oncol. Author manuscript; available in PMC 2006 June 12.
Published in final edited form as:
PMCID: PMC1475951

Adoptive Cell Transfer Therapy Following Non-Myeloablative but Lymphodepleting Chemotherapy for the Treatment of Patients With Refractory Metastatic Melanoma



We investigated the combination of lymphodepleting chemotherapy followed by the adoptive transfer of autologous tumor reactive lymphocytes for the treatment of patients with refractory metastatic melanoma.

Patients and Methods

Thirty-five patients with metastatic melanoma, all but one with disease refractory to treatment with high-dose interleukin (IL)-2 and many with progressive disease after chemotherapy, underwent lymphodepleting conditioning with two days of cyclophosphamide (60 mg/kg) followed by five days of fludarabine (25 mg/m2). On the day following the final dose of fludarabine, all patients received cell infusion with autologous tumor-reactive, rapidly expanded tumor infiltrating lymphocyte cultures and high-dose IL-2 therapy.


Eighteen (51%) of 35 treated patients experienced objective clinical responses including three ongoing complete responses and 15 partial responses with a mean duration of 11.5 ± 2.2 months. Sites of regression included metastases to lung, liver, lymph nodes, brain, and cutaneous and subcutaneous tissues. Toxicities of treatment included the expected hematologic toxicities of chemotherapy including neutropenia, thrombocytopenia, and lymphopenia, the transient toxicities of high-dose IL-2 therapy, two patients who developed Pneumocystis pneumonia and one patient who developed an Epstein-Barr virus-related lymphoproliferation.


Lymphodepleting chemotherapy followed by the transfer of highly avid antitumor lymphocytes can mediate significant tumor regression in heavily pretreated patients with IL-2 refractory metastatic melanoma.


Adoptive cell transfer (ACT) immunotherapy is based on the ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host.1 Murine models of ACT have established the ability of this approach to mediate the regression of established cancers and have provided important principles to guide human studies.2-4In murine models, prior host immunosuppression can dramatically improve the antitumor effects of ACT therapy.5,6 Additional improvements can be achieved by the systemic administration of interleukin (IL)-2 or other cytokines to support the transferred cells, and concurrent immunization with inflammatory formulations of the tumor antigen recognized by the transferred cells.7,8

In the National Cancer Institute (NCI; Bethesda, MD) Surgery Branch, we have developed improved methods for the generation of human tumor-infiltrating lymphocytes (TILs) with antitumor activity9,10 and have administered these TILs in combination with prior immunosuppression and the administration of IL-2.11 We previously reported that six (47%) of 13 patients with IL-2 refractory metastatic melanoma treated with this combination experienced an objective partial response. Two of the responding patients exhibited a transient lymphocytosis composed primarily of the transferred, tumor-reactive TIL cells. These two patients further demonstrated a clonal repopulation of their immune systems, with more than 70% of their peripheral blood lymphocytes (PBLs) consisting of a single tumor reactive clone for over four months after treatment.

In the current study, we extend these results to report on the treatment of a total of 35 patients with refractory metastatic melanoma using selected, rapidly expanded TIL cultures and high-dose IL-2 therapy after nonmyeloablative but lymphodepleting chemotherapy. Eighteen (51%) of the 35 treated patients demonstrated an objective response to treatment, and eight others demonstrated a mixed or minor response. Some patients also exhibited symptoms of autoimmune melanocyte destruction including vitiligo or uveitis. The results and analysis reported here help elucidate many of the principles required for the immune-mediated destruction of established cancers.


Patient Treatments and Clinical Assessment

Patients older than 18 years with stage IV melanoma who were negative for hepatitis B and C and HIV infection and had a good performance status and a life expectancy of at least 3 months were eligible for treatment on this protocol. All patients signed an institutional review board-approved consent and had melanoma that was histologically confirmed by pathologists at the Clinical Center, National Institutes of Health (Bethesda, MD). All patients had assessable disease (measurable disease on computed tomography scan or by physical exam) refractory to standard treatments including high-dose IL-2 therapy (except patient 31, who did not receive IL-2 before entry into this protocol). Five (14.7%) of the 34 patients who received high-dose IL-2 initially responded to IL-2 therapy alone, but then exhibited progressive disease and were enrolled on this protocol. Granulocyte colony-stimulating factor (G-CSF)-mobilized stem cells were obtained by leukapheresis from all patients and cryopreserved before initiation of chemotherapy in the event that patients did not reconstitute their hematopoetic system. Patients received nonmyeloablative lymphodepleting chemotherapy as previously described12,13 consisting of 2 days of cyclophosphamide (60 mg/kg) followed by 5 days of fludarabine (25 mg/m2). On the day following the final dose of fludarabine, patients received cell infusion with tumor-reactive lymphocytes and high-dose IL-2 therapy consisting of 720,000 IU/kg intravenously every 8 hours to tolerance as previously described.14,15 After enrollment of 15 patients, the protocol was amended through the institutional review process so that all subsequent patients who had MART-1-specific16 or gp100-specific17 TIL received vaccination with 1 mg MART-1:26-35(27L) or gp100:209-217(210M) peptide in incomplete Freund’s adjuvant (IFA) injected subcutaneously.

Patients’ hematologic parameters were monitored daily by obtaining complete and differential blood counts, and by flow cytometric analysis of peripheral mononuclear cells. Patient response was assessed using standard radiographic studies and physical examination at four weeks following cell administration and at regular intervals thereafter. A complete response (CR) was defined as the disappearance of all clinical evidence of disease. A partial response (PR) was defined as a 50% or greater decrease in the sum of the products of perpendicular diameters of all measurable lesions for at least one month with no increase in any lesion and no new lesions. Appearance of any new lesion or greater than 25% increase in any lesion following a PR or CR was considered a relapse. A CR or PR was considered to be an objective response, and duration was measured from the initiation of treatment to the time of relapse. Patients with mixed or minor responses or progressive disease were considered nonresponders.

Patient Materials and Cell Lines

TIL cultures for treatment were generated as previously described.9 This clinical trial was not designed to predict response rates on an intent-to-treat basis. Our prior analysis of sequential melanoma biopsies from HLA-A2+ patients demonstrated that tumor specific activity was detectable in TILs from 29 (81%) of the 36 patients screened.9 In brief, the method for TIL generation involved the initiation of multiple independent cultures in 24 well plates, which were maintained at 0.8-1.5 × 106 cells/mL. When sufficient TILs were available, each culture was tested by cytokine-release assay for antigen specificity and tumor reactivity. Active TIL cultures were expanded to treatment levels using a rapid expansion protocol (REP) as previously described18,19 with OKT3(anti-CD3) antibody (Ortho Biotech, Bridgewater, NJ) and IL-2 in the presence of irradiated, allogeneic peripheral-blood mononuclear cells from at least three different donors. The rapidly expanded cultures were typically maintained for 13 to 14 days before patient infusion, and were infused into the patient by intravenous administration over 30 minutes.

Immunologic Assays

TIL activity and phenotype were determined by analysis of cytokine secretion, flourescence activated cell scanning (FACS), and immunocytochemistry as previously described.9,20,21

Statistical Analysis

Significance of variation between groups was evaluated using a two-tailed Student t test assuming equal or unequal variance with P ≤ .05 considered significant.


Patient Characteristics

Thirty-five patients with assessable metastatic melanoma who had TIL cultures that recognized autologous or HLA-matched tumor cells were eligible for treatment on this protocol. The demographic characteristics of this patient population are shown in Table 1. All but two patients (patients 34 and 35) were HLA-A*0201+. All patients had progressive disease after receiving multiple prior therapies before treatment on this protocol, including surgery and (except patient 31) high-dose IL-2 therapy, and 18 of the patients had progressive disease after receiving chemotherapy.

Table 1
Patient Demographics

Each patient had one or more metastatic lesions resected for the generation of lymphocyte cultures and autologous tumor cell targets.9 The activity and specificity of three representative TIL cultures used for treatment are shown in Table 2.All TIL cultures that recognized allogeneic HLA-A2-matched melanoma cells were screened for recognition of MART-1:27-35 and gp100:209-217 peptides.

Table 2
Antigen Specificity and Activity of Representative TIL Cultures Infused for Patient Treatment (IFNγ, pg/mL)

Clinical Results

Individual treatments varied based on the growth rates of cells and patient tolerance of IL-2 administration and are summarized in Table 3.A mean of 6.3 × 1010 cells was infused (range, 1.1 to 16.0 × 1010), comprising from one to four independently expanded TIL cultures per patient.

Table 3
Treatment Characteristics

Eighteen (51%) of the 35 patients experienced objective clinical responses to treatment (three CRs and 15 PRs); their sites of tumor regression and duration of response are listed in Table 4.Eight other patients (23%) experienced mixed or minor responses to treatment. Regression of metastatic melanoma in diverse anatomic locations was seen, including bulky cutaneous and subcutaneous disease; widespread nodal disease in peripheral or central locations (Fig 1A); liver metastases (Fig 1B); multiple sites including brain and axillary disease (Fig 1C); lung and lymph nodes (Fig 1D); and lung and subcutaneous sites (Fig 1E).

Fig 1Fig 1
Lymphodepletion and adoptive cell transfer-induced regression of metastatic disease in diverse anatomic sites. (A) Axillary, pelvic and intraperitoneal metastases (patient 1); (B) extensive liver disease (patient 31); (C) brain and recurrent axillary ...
Table 4
Clinical Responses to Treatment

Patients who were treated with lymphodepleting chemotherapy and ACT therapy and who experienced a minor or mixed response, who had stable disease after treatment, or who progressed after a PR were eligible for an additional course of treatment. Three patients experienced an objective clinical response after the second course of treatment (patients 2, 6, and 17). Fourteen other patients received more than one course of lymphodepleting chemotherapy followed by cell transfer and high-dose IL-2 therapy on NCI Surgery Branch protocols. Eight patients who had a mixed or minor response or stable disease after a first course of treatment were re-treated with a second course with the same or a different TIL, and high-dose IL-2 therapy, but did not achieve an objective clinical response after the second course. Six additional patients had received a primary course of lymphodepleting chemotherapy and high-dose IL-2 therapy with cell transfer in prior Surgery Branch protocols without achieving an objective response before their enrollment in this protocol. These cell treatments included administration of cloned T cells (n = 2), nonrapidly expanded TIL (n = 1), peripheral blood-derived cells (n = 1) or gene-modified cells (n = 2). Five of these six patients experienced an objective clinical response to their second cycle of nonmyeloablative chemotherapy that was administered with rapidly expanded TIL and high-dose IL-2 therapy. In all, eight (47%) of the 17 patients treated with a second course including nonmyeloablative chemotherapy and rapidly expanded TIL experienced an objective response, suggesting that multiple courses of treatment are justified in some patients.

In an attempt to identify characteristics of the administered treatments that could predict patient response, many parameters of treatment were compared between the responding and nonresponding patients (Table 5). Characteristics of the lymphocyte cultures were similar in the two groups, and despite comparison of a large number of variables, no statistical difference was detected in any measured parameter of the cultures. Peptide vaccination after cell transfer also had no apparent effect on treatment efficacy, with eight of 17 nonresponders receiving vaccines (three gp100 and five MART-1) and 10 of 18 responding patients receiving vaccine (three gp100 and seven MART-1). The only statistically significant difference was found to be the number of IL-2 doses tolerated (P = .03, Student t test not corrected for multiple comparisons), with the nonresponding patients receiving more doses (mean, 9.6 ± 0.7) than the responding patients (mean, 7.7 ± 0.5). This difference might reflect an increased capacity for cytokine production or pro-inflammatory activities of the infused lymphocytes in vivo in the responding patients that limited their tolerance to high-dose IL-2.

Table 5
Comparison of Treatments Administered to Patients With OR and NR

Thirteen of the patients who had an objective response to this treatment ultimately progressed at one or more sites after treatment. For nine of these patients, antigen expression by tumor cells was evaluated in pretreatment and recurrent lesions. Recurrent lesions from four patients did not express HLA-A2 and recurrent lesions from one other patient did not express the MART-1 protein (Table 6, Fig 2). These results suggest that ACT therapy exerted a strong selective pressure on the tumor in these patients, and that the transferred cells eliminated antigen-expressing tumor cells by an antigen-dependent mechanism. Ten nonresponding patients had immunocytologically assessable disease before and after treatment; one patient exhibited an apparent loss of antigen expression coincident with cell administration, and two patients had no expression of HLA-A2 before treatment (on retrospective analysis). Seven nonresponding patients expressed HLA, MART-1 and gp100 antigens.

Fig 2
Loss of antigen expression in post-treatment lesions. Tumor cells in pretreatement samples expressed all antigens; however, individual antigens were lost from recurrent lesions. A) MART-1 antigen expression was lost in a recurrent lesion from patient ...
Table 6
Antigen Loss in Recurrent Lesions of Six Patients Revealed by Immunocytochemistry

Toxicity of Treatment

There were no treatment-related deaths. Hematologic toxicities due to the conditioning chemotherapy were transient, and included low RBC and platelet counts requiring transfusions in 34 and 29 patients, respectively (Table 7). Low absolute neutrophil counts (ANCs) and absolute lymphocyte counts, and the extended depression of CD4 lymphocyte counts were observed in most patients. Seven patients experienced opportunistic infections including herpes zoster (n = 3), Pneumocystis carinii (n = 2), and respiratory syncitial virus (n = 1). One patient (the only patient who was seronegative for EBV on entrance into the trial) who experienced greater than 90% regression of cancer and returned to normal function, developed an EBV lymphoproliferative disease four months after treatment and died of this lymphoma eight months later. Several nonhematologic grade 3 and 4 toxicities associated with the lymphodepleting chemotherapy were noted in patients treated with ACT therapy (Table 7). Thirteen patients experienced episodes of febrile neutropenia, and one patient developed cortical blindness secondary to progressive multifocal neuropathy, a rare toxicity associated with fludarabine. Patients also exhibited typical transient toxicities associated with high-dose IL-2 therapy, which were easily managed. There were no grade 3 or 4 toxicities attributable to the administration of antitumor lymphocytes. However, 17 patients exhibited autoimmune melanocyte destruction, including twelve patients with vitiligo, three patients with uveitis, and two patients with both vitiligo and uveitis. Uveitis was treated with topical corticosteroid eye drops and all patients’ vision returned to normal. Autoimmune manifestations of treatment were not significantly correlated with objective antitumor responses, although the correlation for patients who received TIL specific for melanocyte differentiation antigens suggested a trend (two-tailed P = .06).

Table 7
Time in Hospital and Nonhematologic Grade 3 and 4 Toxicities Related to Lymphodepleting Chemotherapy and Cell Transfer

The chemotherapy administered before cell transfer induced a transient depression of neutrophil counts. Because of conflicting information concerning the immunosuppressive impact of G-CSF administration on lymphocyte recovery in allotransplant patients, ten consecutive patients in this study (patients 14 through 23) received cell transfer in the absence of G-CSF administration. No obvious difference in clinical response was observed (4 PR, 6 no response [NR] in the no-G-CSF group compared to 14 PR, 11 NR in the G-CSF group), but G-CSF administration significantly improved neutrophil recovery, as measured by time to recovery of ANC > 500/mm3 (Fig 3) and average ANCs during the recovery phase (not shown).

Fig 3
Granulocyte colony-stimulating factor (G-CSF) administration significantly improved the time to neutrophil recovery (two-tailed P < .03). The percentage of patients who had recovered neutrophil counts above 500/ mm3is graphed. ANC, absolute neutrophil ...

Absolute lymphocyte counts reached their lowest point at a mean value of 17/mm3 (range, 0 to 58 lymphocytes/ mm3) by the day of cell transfer (normal range > 460 cells/mm3). In some patients, a transient lymphocytosis that peaked within the first week after cell transfer was seen (Fig 4). Figure 5shows the analysis of PBL subsets in patients from 1 month to more than 1 year after cell transfer. The average pretreatment CD4+, CD8+, and B-cell counts were within normal limits. However, by 30 to 60 days after treatment, the average CD4+ count fell to 137 cells/mm3, and remained below 200 cells/mm3 for the duration of the study. CD8+ cell counts transiently rose to a maximum of 835 cells/mm3 60 to 90 days after transfer, but decayed to normal levels over time. Like T cells, the B-cell compartment was transiently depleted by the chemotherapy, but B-cell levels returned to the normal range with a few months of treatment in most patients (not shown). Platelet recovery more than 30,000/mm3 and ANC values at normal levels were typically achieved within 2 weeks of cell transfer in the first course of treatment. Interestingly, 17 patients who received a second course of treatment exhibited a significant delay in recovery of platelets, with a median time to recovery increasing from 8 days to 11 days (two-tailed P < 3 × 10-5) and a small (approximately 1 day) delay in neutrophil recovery (two-tailed P = .03). The delayed hematologic recovery after multiple courses of treatment represents a potential impediment to the use of repeated courses of treatment in some patients, although no patient on this study experienced pancytopenia that required administration of their mobilized progenitor cells banked before treatment.

Fig 4
Absolute lymphocyte count (ALC) increased transiently in some patients after adoptive cell transfer. The maximum ALC for each patient within the first 8 days after cell transfer is plotted for all nonresponding patients (NR) and objective responders (OR) ...
Fig 5
CD8+lymphocyte counts recovered more rapidly than CD4+lymphocytes. Each symbol represents an individual patient. All available data is included, but measurements were not available for all patients at each time increment. Open symbols represent five patients ...

Persistence of Transferred Lymphocytes

The long-term persistence of tumor-reactive T cells from the transferred TIL was investigated using fluorochrome-labeled HLA-A2/peptide tetramers or T cell receptor beta chain variable region (TCR Vβ) family specific antibodies. The use of TCR Vβ family-specific antibodies to follow individual T cell clones is hampered by a lack of a specific antibody to approximately one half of all expressed TCR Vβ families, and interpretation of results is also complicated by endogenous reconstituting lymphocytes that can express the same Vβ as the clone of interest. Several of the patients who exhibited an objective response had received a TIL that contained a predominant T cell clone that was recognized by HLA tetramers or Vβ antibodies. Previous reports11,22 demonstrated that a high frequency of the transferred, tumor-reactive clone was maintained in peripheral blood of a few patients for several weeks after cell transfer. We extended these results by evaluating PBLs from select responding patients sampled months to years after treatment. The relative percentage of the transferred tumor reactive clones derived from the TIL varied widely from patient to patient, and four of the five patients with assessable samples had evidence of long-term clonal persistence (Fig 6). The frequency of MART-1-reactive cells in the PBL of patient 28 changed from a pretreatment level below detection (< 0.1%) to 3.8% of circulating CD8+ cells by day 146 after treatment. Similarly, patient 16, whose administered TIL included a tumor reactive, Vβ1-expressing clone (not shown) demonstrated an increase in circulating Vβ1 cells from a pretreatment value of 4.2% to a level of 8.8% of circulating CD8+ cells 23 months after treatment. Patient 21 had extended persistence of a tumor-reactive Vβ22-expressing clone, with an endogenous pretreatment Vβ22 level of 3.9% that increased 403 days after administration of a Vβ22-containing TIL to 25.5% of circulating CD8+ cells. Most strikingly, 810 days after treatment, a MART-1-reactive, Vβ7-expressing clone from the TIL of patient 10 comprised more than 66% of his circulating CD8+ lymphocytes. We are currently investigating a potential correlation between long-term tumor antigen-reactive lymphocyte persistence and tumor regression in patients treated with ACT using sensitive and quantitative nucleotide sequence based approaches including random amplification of cDNA ends (5′-RACE) analysis.

Fig 6
Transferred cells persisted for extended time in responding patients. Each tumor-infiltrating lymphocyte administered on day 0 contained a tumor reactive clone with the indicated T-cell receptor beta chain variable region (Vβ) expression or HLA-A2/MART-1-26-35 ...


The administration of ACT therapy and high-dose IL-2 therapy following lymphodepleting chemotherapy resulted in objective clinical responses in 18 of 35 patients with metastatic melanoma, including 34 patients who were refractory to treatment with high-dose IL-2. Thirteen of the 18 responding patients were also refractory to chemotherapy. Metastatic disease at numerous anatomic sites was susceptible to this therapy, including cutaneous lesions, bulky nodal disease, and visceral, brain, and bony metastases. The toxicities associated with this treatment included the expected toxicities of high-dose IL-2 therapy and the hematologic suppression associated with lymphodepleting chemotherapy; these were mostly transient and readily managed by standard supportive techniques.

Autoimmunity directed against normal melanocytes was observed in nine (75%) of the twelve patients who were treated with T-cell cultures that recognized melanocyte differentiation antigens and who experienced objective tumor regression. Melanocyte destruction in the skin commonly resulted in vitiligo in the responding patients. Similarly, anterior uveitis was detected in four responding patients, and was controlled with corticosteroid eye drops. A link between successful immunotherapy and the onset of autoimmunity has been noted in several clinical immune-based therapies,15,23,24 as well as preclinical immunotherapy models,25 suggesting a strong link between the mechanisms of tumor regression and autoimmune attack on normal tissues. The nature of the melanoma antigen targeted by TIL did not predict the outcome of treatment. Shared tumor antigens such as MART-1 that are expressed by nonmalignant tissues were as effective for targeting melanoma destruction as unique antigens expressed only by the tumor cells. This observation suggests that peripheral mechanisms of self-tolerance (ie, other than thymic deletion) can inhibit the endogenous immune response to tumor, and need to be overcome to induce clinically relevant antitumor responses.

The current protocol incorporated several changes compared to our prior efforts at ACT of patients with melanoma. Improved approaches were developed for the generation of tumor-reactive lymphocyte cultures that often contained multiple clonotypes and a mixture of CD4+ and CD8+ cells.9 In the current trial, 31 treatments contained cells that were selected for tumor recognition and rapidly expanded with OKT3 only a single time, and five treatments (1 PR and 4 NR) contained cells expanded with OKT3 more than once. These cultures contrasted with the unselected TIL26 or the highly selected cloned CD8+ lymphocytes13,18 we administered in prior trials. In previous trials with cloned CD8+ lymphocytes for ACT, the generation of large numbers of cloned cells required at least three expansions mediated by OKT3. The decreased proliferative potential of the cells used in prior trials that were rapidly expanded multiple times in vitro may have accounted for the poor persistence of these cells after cell transfer and their failure to cause tumor regression. A second important change we incorporated into the current protocol was the administration of a conditioning chemotherapy regimen before the cell infusion. This chemotherapy regimen contained agents with little anti-melanoma activity and was designed to deplete endogenous lymphocytes before the cell transfer. The role of lymphodepletion in augmenting the antitumor function of adoptively transferred lymphocytes is an area of active investigation. Two potential mechanisms have been proposed based on studies in preclinical models: the elimination of suppressor T cells,27 or the decreased competition by endogenous lymphocytes for homeostatic regulatory cytokines such as IL-7 or IL-15.7,28,29 The observation of lymphocytosis in some responding patients (Fig 4), together with the suggestion of antitumor T-cell persistence in patients with extended responses (Fig 6) offers hints as to the requirements for successful tumor treatment by ACT therapy. These observations suggest that the capacity for T-cell proliferation in vivo and the transfer into a lymphodepleted host are important determinants of efficacy. More research into these aspects of cell transfer therapy is warranted.

Several variations in administered treatments did not apparently impact the treatment efficacy. Although administration of G-CSF has been reported to be immunosuppressive in patients undergoing allotransplantation of stem cells,30,31 no statistical difference in the response rate was observed in the small, nonrandomized patient populations in this study. Similarly, there were no apparent immunologic or clinical differences between patients receiving a peptide vaccine after cell transfer and those patients who did not receive vaccination. Other aspects of treatment, which are active areas of investigation, include the route of cell administration (intra-arterial v intravenous32), and the dose and schedule of IL-2 administration.33

Some of the patients reported here had extensive tumor regression, but eventually recurred at pre-existing or new sites. Immunocytochemical analysis of pretreatment and recurrent lesions demonstrated a loss of antigen expression by the recurrent tumor in five (55%) of nine patients who relapsed after an objective response. In two patients (patients 2 and 28) the loss of HLA class I antigens observed by immunocytochemical analysis was confirmed by in vitro functional analysis. Melanoma cell lines derived from recurrent tumor biopsies of these patients demonstrated loss of HLA expression (due to mutation of the beta2-microglobulin gene in one patient) and failure to stimulate antigen-specific T cells (not shown). In one patient treated with MART-1 reactive cells, the expression of MART-1 protein was selectively decreased while other melanoma differentiation antigens including gp100 were not affected. These results demonstrate that the transferred T cells exerted a strong selective pressure on the tumor in vivo, and confirm that the antitumor effects of this treatment are antigen mediated.

In summary, the results presented here establish that ACT therapy can impact bulky metastatic melanoma that is refractory to other treatments including high-dose IL-2 therapy and chemotherapy. Fifty-one percent of the patients experienced objective tumor responses with durations from 2 months to more than 2 years, including three patients (9%) with an ongoing complete response. Many aspects of this treatment have the potential to be improved by additional studies of the basic scientific principles of immune-mediated tumor rejection and the optimization of their clinical application. Current studies in our laboratory are focused on the logistical aspects of generating autologous cell-based patient treatments, the genetic modification of lymphocytes with T-cell receptor genes and cytokine genes to change their specificity or improve their persistence, and the administration of antigen specific vaccines to augment the function of the transferred cells.


We are grateful for the expert cell culture, technical, and data processing assistance provided by Linda Parker, Azam Nahvi, Thomas Shelton, Amy Hubicki, Kevin O’Regan, Mary Ganges, Patti Fetsch, and Melissa Corbett. We are also grateful for the expert patient care provided by the immunotherapy nurses and fellows of the Surgery Branch, the nurses and staff of 2 East of the Clinical Center, National Institutes of Health, and the Surgical Intensive Care Units of the Center for Cancer Research, the NCI, and Michael Bishop.


Authors’ Disclosures of Potential Conflicts of Interest The authors indicated no potential conflicts of interest.


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