PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of narLink to Publisher's site
 
Nucleic Acids Res. 1997 November 1; 25(21): 4422–4426.
PMCID: PMC147052

Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA.

Abstract

Methylation analysis of individual cytosines in genomic DNA can be determined quantitatively by bisulphite treatment and PCR amplification of the target DNA sequence, followed by restriction enzyme digestion or sequencing. Methylated and unmethylated molecules, however, have different sequences after bisulphite conversion. For some sequences this can result in bias during the PCR amplification leading to an inaccurate estimate of methylation. PCR bias is sequence dependent and often strand-specific. This study presents a simple method for detection and measurement of PCR bias for any set of primers, and investigates parameters for overcoming PCR bias.

Full Text

The Full Text of this article is available as a PDF (201K).

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press