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Nucleic Acids Res. 1997 October 1; 25(19): 3957–3958.
PMCID: PMC146979

Betaine improves the PCR amplification of GC-rich DNA sequences.


Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.

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Selected References

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