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Nucleic Acids Res. 1996 November 1; 24(21): 4362–4363.
PMCID: PMC146234

An efficient DDAB-mediated transfection of Drosophila S2 cells.


I have developed an efficient method for transfecting Drosophila S2 cells using DDAB, a cationic liposome reagent. The optimized DDAB method resulted in a 10 times or greater increase in transfection efficiency compared with the conventional calcium phosphate method which has been essentially the only way for transfecting S2 cells.

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