The MASTERPLAN study is designed as a multicenter randomized controlled clinical trial with a duration of 5 years of follow-up.
Aims & primary outcome
The MASTERPLAN study aims to show that in patients with mild to moderate CKD strict implementation of current guidelines by a nurse practitioner, with emphasis on the use of cardioprotective medication and lifestyle changes, results in a reduction of a composite endpoint of myocardial infarction, stroke and cardiovascular mortality.
Cardiovascular morbidity, overall mortality, quality of life, percentage of patients achieving treatment goals, changes in renal function and changes in markers of vascular damage will be considered as secondary outcomes.
Changes in renal function will be documented by annually measuring creatinine clearance from a 24-hour urine sample and measurement of the serum creatinine. Quality of life will be assessed through questionnaires that will be filled out by all participants annually. Markers of vascular damage will be recorded annually in a proportion of the patients. These markers address different aspects like endothelial function, arterial compliance and atherosclerosis in various vascular beds. Pulse wave analysis (PWA) and pulse wave velocity (PWV) will be measured in approximately 300 patients. Aortic pulse wave forms and aortic pulse wave velocity are indicators of arterial stiffness. PWV has been shown to be an independent predictor for cardiovascular risk[23
]. PWA and PWV measurements have been validated and can be measured in a reproducible manner[25
]. Carotid intima media thickness (CIMT) evaluation through B-mode sonography will also be measured in approximately 150 patients. Increased CIMT is associated with an elevated risk of cardiovascular disease[24
]. Change in CIMT has been shown to result from risk factor modifications in a variety of populations[26
]. CIMT measurement has been validated and shown to be reproducible[27
Blood pressure will be recorded twice per year using a non-invasive automated oscillometric device. Patients will stay in a supine position for 30 minutes, blood pressure will be recorded every three minutes. The last five measurements will be used for analysis. Conventional office readings using the auscullatory method will also be documented.
Recruitment and screening
All subjects will be recruited from the outpatient nephrology or internal medicine clinic of nine Dutch hospitals. Patients are identified by checking their medical records for compatibility with the inclusion criteria prior to a regular outpatient visit. Renal function will be estimated using the Cockcroft-Gault formula (with a correction for body surface area as of April 15th
2005)(appendix A). The Cockcroft-Gault equation has been extensively validated and is generally appreciated as a useful tool to estimate creatinine clearance[28
]. The physician will give every eligible patient a brief verbal explanation and extensive written information about the study. A week later follow-up by the nurse practitioner will be performed. Upon verbal consent to participate in the study, the patient will be invited to the office. In this visit the in- and exclusion criteria will be checked thoroughly, written informed consent will be acquired and subsequently baseline measurements will be performed. The medical ethics committees of the participating hospitals have approved the conduct of the study.
Recruitment began in April 2004 and is estimated to continue until December 2005. About 60% of patients deemed eligible by their physician and asked to participate in the study, actually participate and are included. The main reasons for non-participation appear to be: reluctance of the patient to changes in drug therapy and inability of the patient to attend the required visits. On December15, 2005 793 patients had been included.
In- and exclusion criteria
Patients are eligible for inclusion when they fulfill the following criteria:
• The subject is at least 18 years old
• The subject is diagnosed with CKD with a creatinine clearance estimated by the Cockcroft-Gault equation between 20 and 70 ml/min.
• The subject is able and willing to provide written informed consent
The following conditions are considered exclusion criteria:
• A renal transplant less than a year before inclusion
• Acute renal failure or rapidly progressive glomerulonephritis established by the treating physician
• Any malignancy less than five years before inclusion other than basocellular or squamous cell carcinoma of the skin.
• Participation in other clinical trials requiring the use of study medication
From April 15th
2005 until the end of the inclusion the Cockcroft-Gault equation was modified to take into account body surface area according to recent insights into the applicability of formulas to estimate renal function [30
]. This modification was approved by the medical ethics committee.
Baseline evaluation and randomization
Table summarizes the data collected at baseline evaluation. After the baseline evaluation, the patient will be randomized to either nurse practitioner care or physician care. Randomization to treatment is stratified by center and renal transplant status using a web-based randomization module and performed in predefined blocks of certain numbers of patients.
To all patients the same set of guidelines and treatment goals, represented in Table and Table 3, apply. Both patients and physicians are provided with information about the beneficial effects of multifactorial risk factor management regardless of treatment allocation. In the intervention group nurse practitioners, supervised by a qualified nephrologist, will actively pursue lifestyle intervention (physical exercise, nutritional counseling, weight reduction and smoking cessation), the use of specified cardioprotective medication and the implementation of current guidelines. The nurse practitioner will check regularly whether treatment goals have been met and when deemed appropriate adjust treatment to achieve target values. Modification of therapy will be executed according to flowcharts that have been derived from current guidelines.
Goals and relevant guidelines for cardiovascular risk factors in MASTERPLAN
Standard medication to reduce cardiovascular risk in MASTERPLAN
Physician care comprises 'usual care' conform the guidelines mentioned in Table . In contrast to the intervention group and in agreement with real life practice no extra incentives to adhere to the guidelines will be supplied.
The intervention group will receive follow up by the nurse practitioner as often as is indicated by the guidelines and the sort of lifestyle intervention the patient receives. Additional data will be collected for trial purposes quarterly as described in table 1. The physician care group will receive an automated non-invasive blood pressure measurement two times a year. Annually each patient will be invited in the office to undergo a series of measurements and to fill out questionnaires. At the other time-points (represented in Table ) present clinical data derived from the medical records will be recorded in the case report form.
The patients will fill out several questionnaires. Firstly quality of life will be assessed using the SF-36 (Dutch version) and EQ-5 D (Dutch version) questionnaires[34
]. Secondly the Short Questionnaire to asses health enhancing physical activity will be given to subjects[36
]. Thirdly a Food questionnaire developed by the Wageningen University will deliver information about the composition of the diet of the subjects[37
The entire study is performed according to the ICH-GCP guidelines, including on-site monitoring. The data will be documented in a case report form, via a validated web-based structure. Investigators will fill out the required data of a visit in an Internet application based on PDF files. Upon completing the form they will start the submission process. Before submission the data are verified and passed through an editing process to check for logical inconsistencies within the data. Any discrepancies need to be corrected before actual submission can take place. The data will be sent to a web server located in the data management center. The data are immediately transferred into a SQL server database.
The questionnaires are all in Teleform format and mailed to the data management center. Filled out questionnaires are scanned and data are immediately transferred to the same database used to store the clinical data. A specified validation procedure has been developed to check inconsistencies and to generate and process queries.
Endpoint collection and evaluation
The primary endpoint has been defined as follows, based on experience in other studies run at the UMCU [38
• Myocardial infarction is defined as evident new ischemic changes on an ECG or an established rise and fall pattern of cardiac enzymes.
• Stroke is defined as characteristic clinical symptoms of stroke accompanied by signs of recent ischemia using an appropriate imaging technique (CT-scan or MRI).
• Cardiovascular mortality is defined as death due to myocardial infarction, stroke, ruptured abdominal aneurysm, and terminal heart failure. Also sudden death will be regarded as part of cardiovascular mortality.
All suspected events, which might contribute to the primary endpoint, will be evaluated by an independent endpoint-committee. The committee will evaluate these events using definitions for the different events which are also used in other Dutch trials [38
Secondary endpoints are collected via clinical data, questionnaires or specified procedures. The SphygmoCor system (pulse wave velocity system and blood pressure analysis system, PWV Inc., Sydney Australia) is used to assess PWV and to analyze the arterial pulse contours. Pulse contours will be obtained by applanation tonometry at the carotid, radial and femoral arteries. Independent investigators blinded for treatment allocation of the subject will perform evaluation of recorded data. CIMT assessment will be performed using B-mode ultrasonography of both carotid arteries with a 7,5-MHz linear array transducer to assess the presence of plaques in the common carotid artery, bifurcation and internal carotid artery. All measurements will be recorded on video and evaluated off-line by independent evaluators blinded for treatment allocation of the patient [40
The nurse practitioners are all qualified nurses with several years of experience and affinity for nephrology. They received extensive education in cardiovascular risk reduction with emphasis on current guidelines, they were familiarized with the contents of the flowcharts and all were uniformly trained in interview techniques to support them in implementing lifestyle-modification and maximizing compliance. Completion of the official Dutch Nurse Practitioner training program was no prerequisite to function as a nurse practitioner in this study.
Statistical and data analysis
To detect a 50% between-group difference in the trial primary endpoint with a pre-estimated 5-year event incidence of 13.5% for the control group [16
], a power of 80% and a two-sided type I error α of 5%, at least 640 patients will have to be included. Taking into account a possible loss to follow-up of 15%, at least 740 patients will be randomized.
Group sequential analysis
Group sequential analysis will be used to evaluate the primary endpoints and to monitor the safety data. Sequential analysis is a statistical approach where one conducts significance tests over time as the data are collected. Sequential analysis and its application in clinical trials has been described extensively by Whitehead [42
]. The general approach is as follows. A null hypothesis H0
and an alternative hypothesis H1
are formulated for a suitable measure θ of treatment difference. For this study with a survival type outcome variable, θ is equal to the logarithm of the hazard ratio (HR). H0
is formulated as "no difference in the occurrence of the primary endpoints between the two trial arms" or θ = 0 (i.e. HR = 1). The alternative hypothesis H1
is formulated as |θ | ≥ log(0.48) = 0.73 group. Two test statistics, Z and V, can be derived depending on the type of response variable. Z is a measure of the treatment difference; for survival data Z is the observed number of events in the control group minus the expected number of events given treatment equivalence (i.e. the number of events that would have occurred if the same proportion of events was found in the intervention group and in the control group).V reflects the amount of information about θ contained in Z; for survival data V is approximately equal to a quarter of the number of events observed. The sequential analysis requires critical boundaries to be specified in advance. These boundaries depend onθ, the type I error α and the power 1-β. For each new group of patients, values of Z and V are calculated and presented graphically by plotting Z against V (see Figure1 for an illustration of a double-sided sequential test). Based on the path of cumulative (Z, V)-points, one of the following three decisions is made:
1) the upper or the lower (continuous) boundary is crossed: stop the data collection and reject the null hypothesis;
2) one of the inner wedge-shaped (dashed) boundaries is crossed: stop the data collection and accept the null hypothesis;
3) continue the data collection: the cumulative data are inadequate to draw a conclusion yet.
With a sequential approach a trial can be stopped earlier, on average, than with a fixed sample size approach. Using a sequential design between 460 and 716 patients would have to be included. (The number of patients to be included can not be fixed beforehand because patient data are analyzed cumulatively and a decision is made to stop or to continue the trial according to the interim results.) Due to the large difference between the duration of the inclusion period and that of the follow-up, it will not be possible to implement this potential benefit in the design of the trial. Although the length of follow-up in this trial in relation to the period scheduled for inclusion does not allow for a reduction in sample size other benefits of group sequential analysis remain present. Earlier clarity on the primary endpoint could allow for earlier stopping of the trial and thus result in saving time and funds for other experiments. Also safety related issues, which are to be monitored one-sided, will possibly be identified earlier and in this way patient safety is guaranteed throughout the trial.
Group sequential (or interim) analyses will be performed using the double triangular test as described by Whitehead [42
] and implemented in the computer program PEST version 4 [43
]. The analyses will be performed by an independent data safety monitoring board (DSMB) consisting of a nephrologist, an internist and a statistician. They meet every 6 months to monitor various aspects of the study, including recruitment, adverse events and interim analyses of the primary endpoints.
The results of the study will be analyzed following the 'intention to treat' principle. This means that subjects will be analyzed according to the group they have been allocated to by the randomization. Results will be presented as Kaplan-Meier curves for the two treatments and the difference between the treatments will be analyzed using a log-rank test. For the primary outcome variables the log-rank test will be adjusted for the effect of the cumulative data analyses. Results will be presented for all cause mortality and CV events separately.
The primary analysis of CIMT progression wiw ll be performed using a linear random coefficient (Laird-Ware) model using real visit days, treatment and clinical center as independent variables. For each participant, the intercept and slope of CIMT change over time is assumed to be a normally distributed random variable with different means for the two treatment groups. The mean slope for the nurse practitioner group will be compared to that for nephrologist-care group using linear contrasts and a 5% significance level. Additional exploratory analyses will evaluate the impact of including baseline IMT, lumen diameter, ultrasound reader, and center as additional co-variates.
The data analytic approach to arrive at the PWV outcome variable is similar to that of the CIMT outcome. Adjustments that will be taken into account in the estimates are changes in MAP and changes in heart rate, since both are closely related to PWV.