Search tips
Search criteria 


Logo of bmcgenoBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genomics
BMC Genomics. 2006; 7: 13.
Published online Jan 24, 2006. doi:  10.1186/1471-2164-7-13
PMCID: PMC1420295
High resolution physical map of porcine chromosome 7 QTL region and comparative mapping of this region among vertebrate genomes
Julie Demars,1 Juliette Riquet,1 Katia Feve,1 Mathieu Gautier,2 Mireille Morisson,1 Olivier Demeure,3 Christine Renard,4 Patrick Chardon,4 and Denis Milancorresponding author1
1Laboratoire de Génétique Cellulaire, INRA, BP52627, 31326 Castanet-Tolosan, France
2Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, 78352 Jouy en Josas, France
3Laboratoire de Génétique animale, INRA, 35042 Rennes, France
4Laboratoire de Radiobiologie et d'Etude du Génome, INRA-CEA, 78352 Jouy en Josas, France
corresponding authorCorresponding author.
Julie Demars: julie.demars/at/; Juliette Riquet: juliette.riquet/at/; Katia Feve: katia.feve/at/; Mathieu Gautier: mathieu.gautier/at/; Mireille Morisson: mireille.morisson/at/; Olivier Demeure: demeure/at/; Christine Renard: crenard/at/; Patrick Chardon: Patrick.chardon/at/; Denis Milan: denis.milan/at/
Received August 2, 2005; Accepted January 24, 2006.
On porcine chromosome 7, the region surrounding the Major Histocompatibility Complex (MHC) contains several Quantitative Trait Loci (QTL) influencing many traits including growth, back fat thickness and carcass composition. Previous studies highlighted that a fragment of ~3.7 Mb is located within the Swine Leucocyte Antigen (SLA) complex. Internal rearrangements of this fragment were suggested, and partial contigs had been built, but further characterization of this region and identification of all human chromosomal fragments orthologous to this porcine fragment had to be carried out.
A whole physical map of the region was constructed by integrating Radiation Hybrid (RH) mapping, BAC fingerprinting data of the INRA BAC library and anchoring BAC end sequences on the human genome. 17 genes and 2 reference microsatellites were ordered on the high resolution IMNpRH212000rad Radiation Hybrid panel. A 1000:1 framework map covering 550 cR12000 was established and a complete contig of the region was developed. New micro rearrangements were highlighted between the porcine and human genomes. A bovine RH map was also developed in this region by mapping 16 genes. Comparison of the organization of this region in pig, cattle, human, mouse, dog and chicken genomes revealed that 1) the translocation of the fragment described previously is observed only on the bovine and porcine genomes and 2) the new internal micro rearrangements are specific of the porcine genome.
We estimate that the region contains several rearrangements and covers 5.2 Mb of the porcine genome. The study of this complete BAC contig showed that human chromosomal fragments homologs of this heavily rearranged QTL region are all located in the region of HSA6 that surrounds the centromere. This work allows us to define a list of all candidate genes that could explain these QTL effects.
Chromosome 7 contains a region of the porcine genome in which the highest number of quantitative trait loci (QTL) have been mapped, affecting most notably growth, fat deposition and carcass composition [1-5]. Among all QTL detected on this chromosome, many have been mapped in an interval spanning the SLA complex in position 7p12-q12. Development of a high resolution porcine gene map and comparison with the human map is a major step towards the identification of the genes responsible for QTL effects. The development of two radiation hybrid panels IMpRH and IMNpRH2 constructed after irradiation of porcine cells at 7.000 and 12.000 rads [6,7] provides tools of choice for such comparative mapping studies at 2 complementary resolutions : 1) one breakage could be observed on at least one hybrid of the panel for markers at an average distance of >50 and 20 Kb, 2) the order of a set of markers can be unambiguously determined, provided that the distance between adjacent markers range from 0.25 to 1.5 Mb on IMpRH panel and from 100 to 600 Kb on IMNpRH2 panel [8].
At first glance, the chromosomal region in which QTL have been mapped on SSC7 seemed identical to the p arm of HSA6 [9,10]. A more detailed study showed that a fragment of ~3.7 Mb found at a pericentromeric location on HSA6 is situated at 23 Mb from the expected location on the porcine genome, precisely in the region of interest [11,12]. This fragment was defined by three genes (FLJ10775, BMP5 and BAG2) located close to the centromere at 56 Mb on HSA6p12.1-6p11.2 and mapped on SSC7q11 close to RAB2L and DAXX genes localized at 33 Mb on HSA6p21.32. A preliminary physical map of this region was proposed by Barbosa [12], but 4 limited BAC contigs were built in this rearranged region. However, it failed to establish a complete comparative map of this region and to ensure that no additional fragments found elsewhere on the human genome are located in the porcine QTL region.
We now report the building of a complete BAC map covering the whole region. This was achieved by taking advantage of the fingerprinting of a 5.5× BAC library (Chardon et al, in preparation), undertaken to establish primary BAC contigs. We monitored the assembly of the complete contig by mapping genes and markers on the high resolution IMNpRH212000 panel, as previously done in the Halothane region [8]. Sequence comparison of BAC ends from this contig with the human sequence allowed us to identify 4 BAC containing the four synteny breaking points detected in this region. A local bovine RH map was also established in this region to determine if the rearrangements observed on the pig genome are specific of suines or if they occurred in an ancestral species. A multispecies comparison of this region was finally performed.
Development of a preliminary porcine gene map
To study the complete rearrangements that occurred in this region of an estimated 3.7 Mb and containing the 3 genes LANO (previously called FLJ10775), BMP5 and BAG2 [11,12], it was necessary to: 1) define the chromosomal blocks arranged differently between HSA6 pericentromeric region and SSC7; 2) determine if additional human fragments moved in this porcine region. In the first approach, we increased the density of genes mapped in this region of the IMNpRH212000 panel [7]. Using the ICCARE browser [13], we identified genes for which porcine EST were available. We chose 5 genes located on HSA6p21.32 and 5 genes located on HSA6p12.1-6p11.2: COL11A2 (33.24 Mb), HSD17B8 (33.28 Mb), RING1 (33.29 Mb), VPS52 (33.33 Mb), RPS18 (33.35 Mb), GCLC (53.47 Mb), TINAG (54.28 Mb), HCRTR2 (55.15 Mb), COL21A1 (56.03 Mb) and DST (56.43 Mb). No gene was selected below PRIM2A since no porcine sequence was available for the few genes located in this region very close to the centromere of HSA6. When possible, primers were developed in the 3' UTR sequence of selected genes (Table (Table1).1). For GCLC only, we had to choose primers in two consecutive exons framing an intron. Markers developed for these 10 genes, as well as markers previously developed [11,12] for RXRb (33.27 Mb), ELOVL5 (53.28 Mb) and PRIM2A (57.29 Mb), were analyzed on the 90 clones of IMNpRH212000 panel. In addition to markers produced in the frame of this project, we included in the analysis markers already mapped in this region on IMpRH and IMNpRH2 panels, in particular HKE4 (33.28 Mb), RAB2L (33.37 Mb), LANO (53.77 Mb) (called previously FLJ10775),BMP5 (55.73 Mb) and BAG2 (57.15 Mb) [11,12]. An average retention frequency of 40% was observed, ranging from 27% for PRIM2A to 55% for RAB2L and HKE4. A draft 1000:1 framework map was then built with Carthagene software [14], using a stepwise locus adding strategy (data not shown). The robustness of the map was tested by comparison of the likelihood of the reference map to the likelihood of maps derived from the reference map by all possible permutations in a window of 6 markers and by inversion of parts of the map using the simulated annealing algorithm. The 6 additional genes (COL11A2, RXRb, RING1, PRIM2A, COL21A1 and RPS18) were mapped at their most likely location relatively to markers of this preliminary framework map, considering the distance between framework markers as fixed.
Table 1
Table 1
Porcine markers developed for RH mapping and BAC contig construction
To compare the order of genes between SSC7 and HSA6, we determined the location of these genes on the NCBI Build 35 assembly of the human genome available at UCSC [15]. We confirmed the insertion in pig of a large fragment between RING1 and VPS52 genes (fragments 1 and 2), which are only 40 Kb apart on the human genome (Figure (Figure1A).1A). The fragment found in pig between these two genes is located on human genome close to the centromere on the HSA6p arm. The mapping of these genes on the porcine RH map allowed us to distinguish two main conserved fragments: one fragment containing BMP5 HCTR2 – Sw2019 – TINAG LANO GCLC (fragment 4), and a second fragment containing DST, BAG2 and PRIM2A (fragment 5). The order of genes is conserved inside each fragment; however, the 2 blocks are oppositely oriented in the porcine and human genomes. A large distance is observed between GCLC and BAG2, suggesting that another fragment is inserted between them, possibly a fragment called "6" in the human genome. This additional porcine fragment is particularly interesting because it contains Sw1856, a marker mapped at the most likely location of the QTL of interest. At that step, it was not possible to determine whether the rearranged fragment includes a short fragment located on the q arm of HSA6. The first gene found on HSA6q arm also mapped on the porcine genome, being FLJ13159 located on SSC1 [11].
Figure 1
Figure 1
Gene based comparative map of SSC 7q1.2 and BTA 23q11 radiation hybrid maps and HSA 6p21.32/HSA 6p12.1-q112 physical map. A- SSC 7q1.2 radiation hybrid map was build using IMNpRH212000 developed by Yerle et al [7]. B- BTA 23q11 radiation hybrid map was (more ...)
Bac contig construction
In order to confirm the rearrangements previously observed between HSA6 (6p21.32 and 6p12.1-11.2) and SSC7 (7q11), we built a complete BAC contig of this region. As in previous studies, an almost complete BAC contig of the SLA region has been developed [12], but a gap remained to be filled precisely in this region that was very different between the human and pig genomes. We screened by PCR the INRA BAC library [16] using primers defined for microsatellites Sw2019 and Sw1856, and for the 10 following genes RING1, HSD17B8, BMP5, HCRTR2, TINAG, LANO, GCLC, PRIM2A, BAG2 and DST. For each of these 10 genes or 2 markers (Sw2019 and Sw1856), we identified one positive BAC (Figure (Figure2).2). To accelerate the establishment of a complete BAC contig, we took advantage of the availability of preliminary BAC contigs built after the fingerprinting of 72 190 BAC of the INRA library (Chardon et al, in preparation). It appeared that 8 of these 12 BAC were mapped in 8 different INRA contigs (contigs 5174, 1178, 5990, 3302, 2270, 137, 236 and 1421), containing from 3 to 21 BAC (Figure (Figure22).
Figure 2
Figure 2
Porcine BAC contig with anchoring on porcine RH map and on human sequence after BAC end sequence analysis. The full contig is presented including mainly INRA BAC. Some additional BAC where recruited on the international analysis available at (more ...)
In the frame of the INRA physical mapping project, 35 000 INRA BAC regularly dispersed on the INRA contigs had been selected. The sequence of their extremities were determined at CNS (Evry, France) and compared with the human sequence (Chardon et al, in preparation). Analysis of 108 porcine BES anchored on the human region between 33 and 34 Mb, or between 53 and 64 Mb, allowed us to identify 55 BAC located on the pig genome in the chosen region. Among these BAC, 39 belonged to one of the 8 INRA contigs already identified. 8 additional BAC allowed us to identify 3 others INRA contigs (contigs 5488, 6382 and 7444) located in this same region, and the last 8 clones correspond to BAC that had not been fingerprinted.
To fill several gaps, the INRA BAC library was screened with markers derived from BES, and 12 additional INRA BAC (bI0149A04, bI0474F10, bI0403D01, bI0842H05, bI0323A08, bI0159D05, bI0438F07, bI1044F02, bI0135F11, bI0311E12, bI0105B03 and bI0032D02) were recruited. For BAC of interest whose BES had not been sequenced by the CNS, we sequenced the extremities in order to get on with the construction of the contig; these sequences were submitted to EMBL Nucleotide Sequence Database (AM156864 to AM156903). To fill the remaining gaps, we selected 7 additional BAC (bE0164A10, RPCI44_0470A18, bE0047O02, bT0079E18, bE0251O22, RPCI_440334P20 and bT220O06) on the international BAC map (Humphrey et al, in preparation, and [17] that were overlapping with BAC already selected. To check for the accuracy of the global contig, new markers were developed using BES. The overlapping of BAC derived from the different INRA contigs was tested by PCR on BAC clones (Table (Table11 and Figure Figure22).
The building of the complete BAC contig was also monitored regularly by the mapping of BAC end- markers on the IMNpRH2 RH panel, verifying that the order of the markers and the distances between them were consistent with the BAC contig results. In particular, markers found at the extremities of the 11 INRA contigs were mapped on the RH panel. For these 19 markers, an average retention frequency of 35% was observed. A 1000:1 framework map was then established, including previously mapped genes and 11 markers derived from BES. The 8 others BAC ends were mapped at their most likely location relatively to markers of the framework. This RH map covering 550 cR is in total agreement with the BAC contig covering the whole region (Figure (Figure22).
Analysis of BAC contig
The availability of BES from BAC located between PRIM2A (fragment 5) and GCLC (fragment 4) permitted characterization of the intermediate fragment in which no genes were mapped (Figure (Figure2).2). All the significant homologies detected with these BES show that this fragment corresponds to fragment 6 located on HSA6 in the interval 57.50 – 63.5 Mb framing the centromere. Among BES anchored at this human region, bI0666E01F is highly homologous to an intron of KHDRBS2, demonstrating the presence of the gene in this porcine region. In addition, the comparison of porcine BES produced from BAC bI0222C06, bI0223C10 and bI0819C05 revealed that these sequences share significant homology with 2 human fragments at a distance of 600 Kb, suggesting the presence of duplication in the human genome. A dot-plot of the human region 57.73 Mb-58.9 Mb on itself was performed with Dotter, using its default settings [18]. The dot-plot showed that this region contains many large repeated sequences. A fragment of 290 Kb located at 57.74 Mb-58.03 Mb is duplicated in position 58.34 Mb-58.63 Mb, and a 50 Kb fragment from 58.07 Mb to 58.12 Mb is duplicated and turned over in position 58.73 Mb-58.77 Mb on HSA6 (results not shown). In the pig, this fragment covers ~40 cR, which corresponds to ~380 Kb suggesting that a single copy is present in the pig genome.
Finally, for BAC bI0688H06 and bI0046E05 mapped in the same region, hits obtained for the two BES of both clones were 650 Kb apart, which is not compatible with the size of a BAC. Analysis of this human region on ENSEMBL database [19] indicates that no porcine BES matches the human genome between 63.05 and 63.55 Mb, whereas BES were anchored in the surrounding region on average each 20 Kb. Altogether these results suggest that a 500 Kb region (in which no genes have already been identified on the human genome) is missing in the pig genome.
From this analysis, an estimate can be made of the size of the rearranged region on the pig genome – considering that the size of this region on the porcine genome is similar to the size of human region also found on the porcine genome. The sizes of the three rearranged fragments are: ~2.5 Mb for fragment 4, 1.8 Mb for fragment 5 and 0.9 Mb for fragment 6. The whole rearranged fragment covers thus ~5.2 Mb of the porcine genome.
To confirm the reality of the rearrangements identified during the building of the BAC contig, we tried to identify a porcine BAC spanning each of the 4 breakpoints, with BES anchored on the 2 expected locations on human genome (Figure (Figure3).3). In order to increase the probability of identifying these BAC, we also selected from the international map [17] BAC overlapping INRA BAC close to synteny breaking points. For BAC with no significant homology, we used FASTA to detect any potential homology, using less stringent parameters. In pig, one BAC anchored on the 2 expected human locations was identified, for the 4 breaking points A-B, C-D, E-F and G-H (bI0149A04 (33.03 Mb – 55.93 Mb), bT0141E11 (53.48 Mb-62.48 Mb), bI0222C06 (63.7 Mb – 57.85 Mb) and RPCI44_0428O15 (56.20 Mb – 33.35 Mb)) (Figure (Figure33).
Figure 3
Figure 3
Description of the synteny breakpoints between SSC7 and HSA6. The arrows represent the various fragments of conserved synteny. The small green arrows symbolize a human duplication of a fragment of 290 Kb. The white box represents a fragment of 500 Kb (more ...)
Analysis of the anchorage on the human map of all BES available in these regions, allowed us to estimate more precisely the location of the 4 breakpoints on the human genome (Figure (Figure3):3): A/H breakpoint is located at 33.26 – 33.35 Mb, I/C at 53.24 – 53.48 Mb, B/G at 55.93 – 56.01, F/D after 58.03 Mb and before 62.46 Mb (with human centromere located in this interval), E point after 63.78 Mb.
Multispecies comparison of the organization of the studied region
To compare the order of genes in this region between various species, we identified the location on the genome of sequenced species of several genes representing each chromosomal fragment described previously: fragment 1 (RXRb, RING1), fragment 2 (VPS52, RPS18 and RAB2L), fragment 3 (ELOVL5), fragment 4 (GCLC, LANO, TINAG and BMP5), fragment 5 (COL21A1, DST and PRIM2A), fragment 6 (KHDRBS2) and fragment 7 (GLULD1). Their locations were determined by blasting the porcine sequences against current genome assemblies: mouse sequence Build 33 assembly from NCBI, dog whole genome shotgun (WGS) assembly v1.0 (July 2004), and chicken galGal2 assembly. To enrich the set of data available for the chicken genome, we developed markers for VPS52 and RAB2L which were not identified on the sequence of chicken genome with the ESTs available for these genes. We mapped these 2 markers on chickRH panel [20] and analyzed the results on ChickRH server [21].
It was also important to include a species phylogenetically close to the pig in the comparison. We selected cattle, a species for which both detailed comparative mapping studies have been performed [22,23] and a partial genomic sequence is available. However, in the region of interest, the assembly of the bovine genome is by no means complete (each gene is found in a different scaffold after blasting the sequence of genes mapped in pig against the bovine genome) and available RH maps are insufficiently detailed. This led us to develop a bovine RH map in this region. We used the ICCARE browser [13], to select bovine EST available for 16 genes of this region: RXRb, RING1, HKE4, HSD17B8, VPS52, RAB2L, ELOVL5, TINAG, LANO, COL21A1, DST, BAG2, PRIM2A, KHDRBS2, PHF3 and PTP4A1 (Table (Table2).2). We developed pairs of primers for each gene and we mapped them on the RH5000 bovine panel [22]. An average retention frequency of 36% was observed, ranging from 26% for GLO1 to 50% for PTP4A1. Vectors obtained for the 16 new genes were analyzed together with 31 markers previously available for BTA23 [22]. A 1000:1 framework map including 10 genes, was built with Carthagene software [14] in the region from PRIM2A to VPS52 (Figure (Figure1B).1B). In addition gene KHDRBS2 is linked with a LOD score 3.5 at the top of this map. Four additional genes HSD17B8, RXRb, RING1, and HKE4 were found totally linked and mapped at their most likely location relatively to markers of the framework (Figure (Figure1B).1B). ELOVL5 was found linked to markers previously mapped in a distal position. PHF3 and PTP4A1 were found significantly linked together (LOD = 13.2), but totally unlinked to other genes.
Table 2
Table 2
Bovine markers developed for RH mapping
In the dog genome, all the selected genes are grouped into two large fragments (fragments 3, 4, 5, 6 and 7 and fragments 1 and 2) located on chromosome 12 (Figure (Figure4).4). In the mouse genome, the same region is similarly split into 3 conserved fragments (Figure (Figure4):4): fragments 5, 6 and 7 are located on MMU1; fragments 3 and 4 on MMU9; and fragments 1 and 2 on MMU17. In chicken, a conserved fragment containing fragments 3, 4, 5, 6 and 7 is located on GGA3. Two other genes, VPS52 and RAB2L (fragment 2), are mapped in a linkage group that also contains markers derived from chicken MHC region located on GGA16 (Figure (Figure4).4). No information is available for RXRB and RING1 (fragment 1). If the order of genes is thus globally conserved in these species, a very different situation is observed on the bovine genome (Figure (Figure4):4): 14 of the 16 selected genes are localized on BTA23, but in an order very different from the generally conserved order, and 2 others genes (PHF3 and PTP4A1 representing fragment 7) are located on chromosome 9.
Figure 4
Figure 4
Multi-species (Gallus gallus, Sus scrofa, Bos taurus, Canis familiaris, Mus musculus and Homo sapiens) comparative map. Data concerning Canis familiaris, Gallus Gallus, Mus musculus, and Homo sapiens result respectively from Dog Jul. 2004 (canFam1) assembly, (more ...)
The analysis of these results shows that the whole fragment (comprising fragments 4, 5 and 6) inserted on the pig genome between fragments 1 and 2 (RING1 and VPS52) seems very well conserved in chicken, dog, mouse and human. In cattle and pig, belonging both to the clade of Cetartiodactyles, fragments 4, 5 and 6 are found at a different location. However, in this 5.2 Mb large fragment, a total conservation of gene order was observed between human and cattle genomes. A global analysis of the chromosomal rearrangements that occurred during evolution was carried out using GRIMM. [24]. The results obtained are summarized on Figure Figure44.
The study of porcine chromosome 7 is of particular interest due to the presence of many QTL affecting traits of economic importance and of MHC [1,2]. One approach to study this chromosome is to identify all the genes located on this chromosome and to compare their order relatively to that in the human genome. Previously, a strong conservation of gene order was found between a large fragment of porcine SSC7 and human HSA6 p arm [9,10]. We had highlighted the existence of a fragment estimated to be less than 4 Mb rearranged between SSC7 and HSA6 [11]. This fragment defined by 3 genes (LANO previously named FLJ10775, BMP5 and BAG2) located on HSA6p12.1-6p11.2 is found in the pig genome between genes located on HSA6p21.32 [11]. The building of a BAC contig in the chromosomal region surrounding SLA complex provided additional information, even if the contig remained incomplete [12]. This study describes a situation that is even more complex, suggesting the presence of VPS52 (previously named SACM2L) within the rearranged region. In the pig genome, this rearranged chromosomal region is included in the region where the QTL of interest is mapped, between markers Sw1856 and NFY (Demeure et al, JAS, in press). In order to identify all candidate genes that may be responsible for the QTL effects, it was particularly important to establish a detailed comparative mapping analysis of this region.
We first built a dense porcine RH map (Figure (Figure1A)1A) using the high resolution IMNpRH212000rad panel, allowing us to order unambiguously genes at distances of 100 to 600 Kb [7]. Our data confirmed the shift of the rearranged fragment and its precise location in the pig genome between RING1 and VPS52 genes (previously called SACM2L) (fragments 1 and 2). In contrast to Barbosa et al [12], we show that VPS52 is located close to RAB2L in both species. We also determined more precisely the extent of the fragment rearranged between the human and pig genomes. The upper frontier of this fragment is located between ELOVL5 (previously named HELO1) and GCLC, located respectively at 53.28 and 53.43 Mb on HSA6. The lower boundary of the fragment is defined by the first gene located on HSA6q also mapped on SSC1. This first gene was previously designated as FLJ13159, and was located at 71.53 Mb on HSA6 [11]. We showed that GLULD1, located at 64.05 Mb on HSA6, also mapped on SSC1 and constitutes the limit currently identified in this fragment.
The gene-based RH map (Figure (Figure1A)1A) that we built allowed us to define 3 sub-fragments of this region arranged differently on the pig and human genomes. Two of these sub-fragments are delimited by genes: BMP5 and GCLC for the first one (fragment 4), and PRIM2A and COL21A1 for the second (fragment 5). No genes were identified in the third fragment (fragment 6), which contains the anonymous microsatellite Sw1856. This map greatly refines the internal rearrangements suggested by our previous work [11]. The sum of the sizes of fragments 4 and 5 is ~4.3 Mb and is similar to the orthologous human region. Fragment 6 covers 1.4 Mb in the human map, whereas the size of this fragment in the porcine RH map suggests a size of approximately 900 Kb which would indicate that a fragment of 500 Kb is absent in the porcine genome. The fragment containing Sw1856 on the pig genome might thus be orthologous to part of the region flanking the centromere of HSA6, but at that step it remained to be proved (Figure (Figure1A1A).
To confirm the order of genes suggested by RH mapping and study this region in more detail, we built a contig of BAC covering the entire region (Figure (Figure2).2). The availability of elementary contigs resulting from the fingerprinting of clones from the INRA BAC library and sequences of extremities of 35000 of these BAC (BES) (Chardon et al, in preparation) greatly facilitated the establishment of this complete contig. Our work completes the studies begun by Barbosa et al [12], who presented 4 small contigs. The analysis of porcine BES anchoring on the human genome demonstrates that the fragment 6 containing porcine marker Sw1856 corresponds to a fragment framing the centromere of HSA6 (6p11.1-6q11.1). Taking into account the average distance between two hits of porcine BES on human genome, we can say that if additional fragments from other human chromosomes are located in this rearranged region of the porcine genome, they should measure <50 Kb.
Analysis of the anchoring of porcine BES on the human genome highlighted also additional results: 1) It was indeed possible to define the position of the 3 synteny breakpoints (Figure (Figure3).3). On the human genome, these synteny breakpoints (A-H, I-C and B-G) are situated between genes RING1-VPS52, ELOVL5-GCLC and BMP5-COL21A1. Each of these 3 synteny breakpoints are localized in a interval of ~100–200 Kb, between the human positions 33.26 Mb-33.35 Mb, 53.24 Mb-53.48 Mb and 55.93 Mb-56.01 Mb. 2) The fourth synteny breakpoint (E-F on the pig genome and F-D on the human genome) was identified thanks to the anchoring of the BES in the human genome: the two fragments framing HSA6 centromere were found in a different order on the pig genome without the presence of a centromere (Figure (Figure3).3). 3) For several BES, alignment on the human genome showed 2 possible positions with similar likelihood. This result suggested the existence of duplicated sequences close to the human centromere, which was confirmed by a dot plot on itself of the sequence of this human region. Phylogeny studies of human chromosome 6 demonstrated the repositioning of this centromere among primate species in a region equivalent to 6p22.1 in Eulemur macaco, Callithrix jacchus and Lagothrix lagothrica, to 6q22 in Macaca fascicularis and Presbytis cristata, and to 6p12 in the great apes [25]. The repeated sequences we highlighted might then correspond to sequences of an ancestral centromere positioned in HSA6p22.1 [25]. 4) Lastly, in silico analysis of the BES alignments on the human genome provided evidence of the absence from the pig genome of a fragment of ~400–500 Kb found in human genome. One can argue that the 2 BES of BAC bI0688H06 match on the human genome with sequences distant of 650 Kb (at 63.02 Mb and 63.67 Mb). The absence of anchoring of other BES between 63.05 Mb and 63.55 Mb, whereas one hit is found for each 20 Kb in the surrounding region, confirmed that this fragment is absent from the pig genome.
This region appears to be heavily rearranged between the porcine and human genomes. We wanted to determine if these rearrangements are specific of suines or Cetartiodactyles, or occur in many other species. To identify gene order in other species, we used the genomic sequence when available. For Gallus gallus, the assembly of the genome has not been totally completed; this is why we enriched information obtained from the sequence by RH mapping data when it was necessary (Figure (Figure4).4). The order of genes seems to be conserved between the chicken and human genomes except for the RXRb and RING1 genes (belonging to fragment 1). These 2 genes which may not exist in the chicken genome are located in other species in the extended class II region of MHC. The chicken MHC, named the B locus, is a 92 Kb DNA sequence containing 19 genes [26,27]. It defines a minimal essential set of MHC genes conserved over 200 million years of divergence between birds and mammals [27].
As the available assembly of the cow genome consists of many independent sequence scaffolds, we determined the gene order by RH mapping [22] (Figure (Figure1B).1B). Our results reveal that genes from GCLC to KHDRBS2 (fragments 4, 5 and 6) are similarly ordered on the human and bovine genomes in this region, which suggests that the internal rearrangements of these 3 fragments, comparing the human and porcine genomes, are specific to the evolution of the suine family. Everts-van der Wind et al. [18] reported that 12 cattle chromosomes are entirely similar to a complete human chromosome arm, and in particular, that p and q arms of HSA6 are homologs of BTA23 and BTA9. Our study modifies slightly this report, in that KHDRBS2 (belonging to fragment 6), the first gene localized on the long arm of human chromosome 6, is probably located on bovine chromosome 23. As the number of genes in this region, as in other species, is very limited; it is difficult to develop and map markers in this region. The end of BTA23 might thus signal the location of the centromere in the ancestors of Cetartiodactyles, in which the fission between the 2 chromosomal arms occurred. In this region, a further comparison of the bovine genomic sequence with the human sequence will be of interest.
We compared the gene order in the pig (Sus scrofa) and cattle (Bos Taurus) with the situation in the genomic sequence of Mus musculus, Canis familiaris, Gallus gallus and Homo sapiens (Figure (Figure4).4). We observed at first that on the pig genome, a large fragment (comprising fragments 4, 5 and 6) of ~5.2 Mb is shifted between RING1 and VPS52, 2 genes belonging to the extended class II region of the MHC. If MHC is one of the most dynamic regions of the genome [28], a high level of conservation of genome organization provides evidence in this region of extended class II genes [29]. Fourteen genes occur in this region between RING1 and the VPS52 in dog, cat, human and mouse genomes, which only differs in the presence of processed pseudogenes [29]. This small fragment of 500 Kb containing RING1 and VPS52 is thus dynamically involved in chromosomal evolution. It is interesting to note that the translocation of the 5.2 Mb fragment occurs in this region. In this region, the genomic organization of the fragment and its flanking regions is similar in mouse, human, dog and chicken, whereas translocation is observed in pig and cattle. This suggests that this genomic reorganization occurred after the divergence of the common ancestor of Cetartiodactyles from the Ferungulates ancestor between 94 to 62 million years ago [30]. The gene order observed in pig for the 5.2 Mb fragment is different from the one identified in other species, indicating that these internal rearrangements occurred after ruminant-suine speciation. We identified a situation really different from the map presented by Everts-van der Wind et al. for the proximal part of BTA23 close to the centromere [23]. Further study of the rearrangements that occurred in ruminants in this region of extended class II of the MHC complex is clearly needed.
We used GRIMM to compare the different maps available and to present a possible evolution of this region between the various species [24]. The unrooted tree we obtained (Figure (Figure4)4) presents a very different view from a classical phylogenic tree [31,32], highlighting a high rate of rearrangements in this region that have occurred recently in Cetartiodactyles. The study of local rearrangements also provides a view different from the recent multispecies comparison of this region [33]. In order to filter out noise induced by mapping errors, this analysis from Murphy et al only took into account fragments >3 Mb, defined by three genes. Among other results, they documented the reuse of evolutionary breakpoint regions identified between conserved homologous synteny blocks. Our study indicates that such a study should be redone on a more detailed scale, when the accuracy of mapping data is adequate.
A QTL affecting fattening and growth had been mapped in the porcine region SSC 7q1.2. As we had shown that a small fragment is translocated between porcine and human genome, it was important to establish a final comparative map of this region. The RH map and the BAC contig we developed in this region allowed us to attest that the translocated fragment is approximately 5.2 Mb long. It consists in three blocks, found in HSA6 between 53.45 and 63.5 Mb, one of these blocks containing a few genes, framing the centromere on the human chromosome. The multispecies study allowed us to confirm that the translocation of this 5.2 Mb fragment occurred in the common ancestor of pig and ruminants, whereas internal rearrangements are found only in the pig genome. The exact knowledge of the genomic content of this region permits us now to establish a list of all possible candidate genes that may affect the growth and fattening of pigs.
Markers and PCR amplification
The ICCARE (Interspecific Comparative Clustering and Annotation foR Ests) tool available at [13] allowed us to select 16 genes in the region between RXRB and RAB2L, for which a porcine EST sequence is available (Table (Table1).1). In addition, 88 markers have been developed from INRA BAC ends. Several result from personal sequencing using the BigDye Terminator V3.1 cycle sequencing kit (Applied Biosystems) on the ABI 3700 automatic sequencer; these sequences were submitted to EMBL Nucleotide Sequence Database (AM156864 to AM156903) [34]. Others come from BAC ends sequences available on ENSEMBL [19]. Primers have been chosen using Primer3 software [35]. PCR was performed in a 15 μl reaction volume containing 25 ng template DNA, 200 μM dNTP, 0.25 μM specific primer pair and 0.5 U Taq polymerase (GoTAQ) in the reaction buffer supplied by the manufacturer. Amplifications were carried out on a Gene-Amp System 9700 (Applied Biosystems) thermocycler. Thermal cycling parameters were defined as follows: denaturation at 94°C for 5 min, followed by 32 cycles of (1) 94°C for 45 sec, (2) annealing temperature for 45 sec, (3) 72°C for 45 sec, and a final extension was performed at 72°C for 5 min. PCR products were analyzed on a 2% agarose gel, and visualized after ethidium bromide staining.
RH mapping
Markers were mapped on the IMpRH panel [6] and IMNpRH2 panel [7] according to the INRA protocols. For markers used on IMpRH panel, vectors were submitted to the IMpRH web server at [36] for an initial two-point assignment. Then, vectors obtained on IMpRH and IMNpRH2 were analyzed with Carthagene software [14]. A 1000:1 framework map was built with buildfw option using a stepwise locus adding strategy under an haploid model of fragment retention. The different provisional frameworks were checked using the simulated annealing algorithm to test inversion of the map fragments, and the flips algorithm to test all local permutations in a window of 6 markers. The resulting RH map was drawn with Map-chart 2.0 [37]. An RH map was also established for the bovine genome using the bovine RH panel RH5000 [22]. Several markers were also mapped on the chicken genome using ChickRH6 panel [20].
BAC screening and chromosome walking
The INRA BAC porcine library [12], was screened by PCR on BAC super pools and pools. The presence of the expected sequence in the identified BAC was checked by PCR on DNA from the isolated BAC clone. For the selected BAC that had been previously fingerprinted and incorporated in a primary INRA contig (Chardon et al in preparation), all BAC from this contig were selected from INRA BAC Server [38]. To confirm the BAC overlapping, markers developed from BES were tested against DNA of BAC belonging to its primary contig or BAC located at the extremities of adjacent contigs.
Multispecies comparison
In order to determine genes order in other species, 12 genes (RXRB, RING1, VPS52, RAB2L, ELOVL5, GCLC, BMP5, COL21A1, DST, PRIM2A, KHDBRS2, GLULD1) were located with Blast in human by using NCBI build 35 produced by the International Human Genome Sequencing Consortium, in mouse (Mus musculus) draft genome data was obtained from the Build 33 assembly by NCBI, in chicken (Gallus gallus) draft assembly was produced by the Genome Sequencing Center at the Washington University School of Medicine in St. Louis, in dog (Canis familiaris) whole genome shotgun (WGS) assembly v1.0 was sequenced and assembled by the Broad Institute of MIT/Harvard and Agencourt Bioscience. We used the GRIMM software [24] to estimate the rearrangements between species and build a multispecies phylogenetic tree.
List of abbreviations
MHC major histocompatibility complex; QTL quantitative trait locus; SLA swine leucocyte antigen; RH radiation hybrid; BAC bacterial artificial chromosome; BES BAC end sequence, cR centi Ray; HSA human chromosome; SSC porcine chromosome; MMU murine chromosome; BTA bovine chromosome; GGA chicken chromosome; CFA dog chromosome; IMNpRH2 INRA Minnesota Nevada porcine Radiation Hybrid panel 2.
BAC bIxxxZxx are from INRA BAC library [12], BAC bExxxZxx are from CHORI242 library (P. de Jong, et al., unpubliched), BAC bTxxxZxx are from Roslin BAC library [39], RPCI44_xxxZxx are BAc clones from RPCI44 library (P. de Jong et al., unpublished).
JD built the complete contig using the INRA BAC library fingerprinting, did the genotyping of the porcine and bovine RH panels, made the in silico analyses and drafted the manuscript. JR supervised this work. KF took part in the development and the explanation of the techniques. MG provided DNA of bovine RH5000 panel and the vectors for the bovine markers previously mapped in the studied region. MM did the genotyping and the analyses on the chicken RH panel. CR performed some preliminary BAC screening. PC coordinated the fingerprinting of INRA BAC and the providing of all BAC necessary to the construction of the contig. DM conceptualized the investigation, analyzed the RH data, and finalized the manuscript.
We would like thank Laurent Schibler, Anne Roig and Jean Claude Save for providing the fingerprints of BAC clones of INRA library, F. Puimi, and the CRB GADIE (Jouy en Josas, France) for providing the BAC clones. We also thank the CNS (Evry, France) for the BAC ends sequencing, and T. Faraut and P. Dehais for help in sequence comparison. JD is supported by a PhD grant from the FSE (Fonds Social Européen).
  • Milan D, Bidanel JP, Iannuccelli N, Riquet J, Amigues Y, Gruand J, Le Roy P, Renard C, Chevalet C. Detection of quantitative trait loci for carcass composition traits in pigs. Genet Sel Evol. 2002;34:705–728. doi: 10.1051/gse:2002026. [PMC free article] [PubMed] [Cross Ref]
  • Bidanel JP, Milan D, Iannuccelli N, Amigues Y, Boscher MY, Bourgeois F, Caritez JC, Gruand J, Le Roy P, Lagant H, Quintanilla R, Renard C, Gellin J, Ollivier L, Chevalet C. Detection of quantitative trait loci for growth and fatness in pigs. Genet Sel Evol. 2001;33:289–309. doi: 10.1051/gse:2001120. [PMC free article] [PubMed] [Cross Ref]
  • Wada Y, Akita T, Awata T, Furukawa T, Sugai N, Inage Y, Ishii K, Ito Y, Kobayashi E, Kusumoto H, Matsumoto T, Mikawa S, Miyake M, Murase A, Shimanuki S, Sugiyama T, Uchida Y, Yanai S, Yasue H. Quantitative trait loci (QTL) analysis in a Meishan x Gottingen cross population. Anim Genet. 2000;31:376–384. doi: 10.1046/j.1365-2052.2000.00696.x. [PubMed] [Cross Ref]
  • Rattink AP, De Koning DJ, Faivre M, Harlizius B, van Arendonk JA, Groenen MA. Fine mapping and imprinting analysis for fatness trait QTLs in pigs. Mamm Genome. 2000;11:656–661. doi: 10.1007/s003350010117. [PubMed] [Cross Ref]
  • Malek M, Dekkers JC, Lee HK, Baas TJ, Prusa K, Huff-Lonergan E, Rothschild MF. A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition. Mamm Genome. 2001;12:637–645. doi: 10.1007/s003350020019. [PubMed] [Cross Ref]
  • Yerle M, Pinton P, Robic A, Alfonso A, Palvadeau Y, Delcros C, Hawken R, Alexander L, Beattie C, Schook L, Milan D, Gellin J. Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet. 1998;82:182–188. doi: 10.1159/000015095. [PubMed] [Cross Ref]
  • Yerle M, Pinton P, Delcros C, Arnal N, Milan D, Robic A. Generation and characterization of a 12,000-rad radiation hybrid panel for fine mapping in pig. Cytogenet Genome Res. 2002;97:219–228. doi: 10.1159/000066616. [PubMed] [Cross Ref]
  • Martins-Wess F, Milan D, Drogemuller C, Vobeta-Nemitz R, Brenig B, Robic A, Yerle M, Leeb T. A high resolution physical and RH map of pig chromosome 6q1.2 and comparative analysis with human chromosome 19q13.1. BMC Genomics. 2003;4:20. doi: 10.1186/1471-2164-4-20. [PMC free article] [PubMed] [Cross Ref]
  • Genet C, Renard C, Cabau C, Rogel-Gaillard C, Gellin J, Milan D. In the QTL region surrounding porcine MHC, gene order is conserved with human genome. Mamm Genome. 2001;12:246–249. doi: 10.1007/s003350010261. [PubMed] [Cross Ref]
  • Tanaka M, Matsumoto T, Yanai S, Domukai M, Toki D, Hayashi T, Kiuchi S, Yasue H, Uenishi H, Kobayashi E, Awata T. Conservation of the syntenies between porcine chromosome 7 and human chromosomes 6, 14 and 15 demonstrated by radiation hybrid mapping and linkage analysis. Anim Genet. 2003;34:255–263. doi: 10.1046/j.1365-2052.2003.00999.x. [PubMed] [Cross Ref]
  • Demeure O, Renard C, Yerle M, Faraut T, Riquet J, Robic A, Schiex T, Rink A, Milan D. Rearranged gene order between pig and human in a QTL region on SSC 7. Mamm Genome. 2003;14:71–80. doi: 10.1007/s00335-002-3034-1. [PubMed] [Cross Ref]
  • Barbosa A, Demeure O, Urien C, Milan D, Chardon P, Renard C. A physical map of large segments of pig chromosome 7q11-q14: comparative analysis with human chromosome 6p21. Mamm Genome. 2004;15:982–995. [PubMed]
  • Muller C, Denis M, Gentzbittel L, Faraut T. The Iccare web server: an attempt to merge sequence and mapping information for plant and animal species. Nucleic Acids Res. 2004;32:W429–34. [PMC free article] [PubMed]
  • de Givry S, Bouchez M, Chabrier P, Milan D, Schiex T. CARHTA GENE: multipopulation integrated genetic and radiation hybrid mapping. Bioinformatics. 2005;21:1703–1704. doi: 10.1093/bioinformatics/bti222. [PubMed] [Cross Ref]
  • UCSC GB.
  • Rogel-Gaillard C, Bourgeaux N, Billault A, Vaiman M, Chardon P. Construction of a swine BAC library: application to the characterization and mapping of porcine type C endoviral elements. Cytogenet Cell Genet. 1999;85:205–211. doi: 10.1159/000015294. [PubMed] [Cross Ref]
  • Sonnhammer EL, Durbin R. A dot-matrix program with dynamic threshold control suited for genomic DNA and protein sequence analysis. Gene. 1995;167:GC1–10. doi: 10.1016/0378-1119(95)00714-8. [PubMed] [Cross Ref]
  • Morisson M, Lemiere A, Bosc S, Galan M, Plisson-Petit F, Pinton P, Delcros C, Feve K, Pitel F, Fillon V, Yerle M, Vignal A. ChickRH6: a chicken whole-genome radiation hybrid panel. Genet Sel Evol. 2002;34:521–533. doi: 10.1051/gse:2002021. [PMC free article] [PubMed] [Cross Ref]
  • ChickRH WS.
  • Williams JL, Eggen A, Ferretti L, Farr CJ, Gautier M, Amati G, Ball G, Caramorr T, Critcher R, Costa S, Hextall P, Hills D, Jeulin A, Kiguwa SL, Ross O, Smith AL, Saunier K, Urquhart B, Waddington D. A bovine whole-genome radiation hybrid panel and outline map. Mamm Genome. 2002;13:469–474. doi: 10.1007/s00335-002-3001-x. [PubMed] [Cross Ref]
  • Everts-van der Wind A, Kata SR, Band MR, Rebeiz M, Larkin DM, Everts RE, Green CA, Liu L, Natarajan S, Goldammer T, Lee JH, McKay S, Womack JE, Lewin HA. A 1463 gene cattle-human comparative map with anchor points defined by human genome sequence coordinates. Genome Res. 2004;14:1424–1437. doi: 10.1101/gr.2554404. [PubMed] [Cross Ref]
  • Tesler G. GRIMM: genome rearrangements web server. Bioinformatics. 2002;18:492–493. doi: 10.1093/bioinformatics/18.3.492. [PubMed] [Cross Ref]
  • Eder V, Ventura M, Ianigro M, Teti M, Rocchi M, Archidiacono N. Chromosome 6 phylogeny in primates and centromere repositioning. Mol Biol Evol. 2003;20:1506–1512. doi: 10.1093/molbev/msg165. [PubMed] [Cross Ref]
  • Guillemot F, Billault A, Pourquie O, Behar G, Chausse AM, Zoorob R, Kreibich G, Auffray C. A molecular map of the chicken major histocompatibility complex: the class II beta genes are closely linked to the class I genes and the nucleolar organizer. Embo J. 1988;7:2775–2785. [PubMed]
  • Kaufman J, Milne S, Gobel TW, Walker BA, Jacob JP, Auffray C, Zoorob R, Beck S. The chicken B locus is a minimal essential major histocompatibility complex. Nature. 1999;401:923–925. doi: 10.1038/44856. [PubMed] [Cross Ref]
  • Kelley J, Walter L, Trowsdale J. Comparative genomics of major histocompatibility complexes. Immunogenetics. 2005;56:683–695. doi: 10.1007/s00251-004-0717-7. [PubMed] [Cross Ref]
  • Debenham SL, Hart EA, Ashurst JL, Howe KL, Quail MA, Ollier WE, Binns MM. Genomic sequence of the class II region of the canine MHC: comparison with the MHC of other mammalian species. Genomics. 2005;85:48–59. doi: 10.1016/j.ygeno.2004.09.009. [PubMed] [Cross Ref]
  • Springer MS, Murphy WJ, Eizirik E, O'Brien SJ. Placental mammal diversification and the Cretaceous-Tertiary boundary. Proc Natl Acad Sci U S A. 2003;100:1056–1061. doi: 10.1073/pnas.0334222100. [PubMed] [Cross Ref]
  • Bourque G, Pevzner PA, Tesler G. Reconstructing the genomic architecture of ancestral mammals: lessons from human, mouse, and rat genomes. Genome Res. 2004;14:507–516. doi: 10.1101/gr.1975204. [PubMed] [Cross Ref]
  • Bourque G, Pevzner PA. Genome-scale evolution: reconstructing gene orders in the ancestral species. Genome Res. 2002;12:26–36. [PubMed]
  • Murphy WJ, Bourque G, Tesler G, Pevzner P, O'Brien SJ. Reconstructing the genomic architecture of mammalian ancestors using multispecies comparative maps. Hum Genomics. 2003;1:30–40. [PubMed]
  • Primer3 S.
  • Milan D, Hawken R, Cabau C, Leroux S, Genet C, Lahbib Y, Tosser G, Robic A, Hatey F, Alexander L, Beattie C, Schook L, Yerle M, Gellin J. IMpRH server: an RH mapping server available on the Web. Bioinformatics. 2000;16:558–559. doi: 10.1093/bioinformatics/16.6.558. [PubMed] [Cross Ref]
  • Voorrips RE. MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered. 2002;93:77–78. doi: 10.1093/jhered/93.1.77. [PubMed] [Cross Ref]
  • Server INRABACW.
  • Anderson SI, Lopez-Corrales NL, Gorick B, Archibald AL. A large-fragment porcine genomic library resource in a BAC vector. Mamm Genome. 2000;11:811–814. doi: 10.1007/s003350010155. [PubMed] [Cross Ref]
Articles from BMC Genomics are provided here courtesy of
BioMed Central