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Nucleic Acids Res. 2006; 34(6): 1676–1684.
Published online 2006 March 22. doi:  10.1093/nar/gkl090
PMCID: PMC1409817

A TFIIB-like protein is indispensable for spliced leader RNA gene transcription in Trypanosoma brucei

Abstract

The lack of general class II transcription factors was a hallmark of the genomic sequences of the human parasites Trypanosoma brucei, Trypanosoma cruzi and Leishmania major. However, the recent identification of TFIIA as part of a protein complex essential for RNA polymerase II-mediated transcription of SLRNA genes, which encode the trans splicing-specific spliced leader RNA, suggests that trypanosomatids assemble a highly divergent set of these factors at the SLRNA promoter. Here we report the identification of a trypanosomatid TFIIB-like (TFIIBlike) protein which has limited overall sequence homology to eukaryotic TFIIB and archaeal TFB but harbors conserved residues within the N-terminal zinc ribbon domain, the B finger and cyclin repeat I. In accordance with the function of TFIIB, T.brucei TFIIBlike is encoded by an essential gene, localizes to the nucleus, specifically binds to the SLRNA promoter, interacts with RNA polymerase II, and is absolutely required for SLRNA transcription.

INTRODUCTION

Initiation of transcription by eukaryotic RNA polymerase (pol) II is a highly conserved process mediated by a set of general transcription factors (13). The core factors required for this process are the TATA-binding protein (TBP) which binds to the TATA box, nucleating the formation of a pre-initiation complex, and the transcription factor (TF)IIB, which interacts with promoter DNA, TBP, the general factor TFIIF and RNA pol II to recruit the polymerase to the correct transcription initiation site (2). TFIIB harbors three distinct domains: an N-terminal zinc ribbon, which binds to the dock domain of RNA pol II and consists of a zinc binding motif and three anti-parallel beta strands (46), the B finger which reaches into the active center of RNA pol II, and a C-terminal core domain which consists of two cyclin repeats facilitating the interaction with TBP and the promoter DNA (79). TBP and TFIIB have conserved orthologs in archaean prokaryotes in which the single RNA polymerase is directed to the transcription initiation site according to the same principle (10,11). Trypanosomatids, a family of unicellular flagellated parasites, appear to represent an exception to the above scheme. While these organisms harbor a single TBP homologue termed TBP-related factor 4 (TRF4) (12), analysis of the completed genomes from the human parasites Trypanosoma brucei, Trypanosoma cruzi and Leishmania major, also known as the Tritryp parasites, revealed no orthologs of the general transcription factors TFIIA, TFIIB, TFIIE and TFIIF (1315). Trypanosomatid protein coding genes are arranged in tandem in directional gene clusters which are transcribed in a polycistronic manner (16,17). This leads to large precursor RNAs from which individual mRNAs are processed by spliced leader (SL) trans splicing and polyadenylation. In trans splicing, the same SL, which is derived from the 5′ terminus of the small nuclear SL RNA, is fused to the 5′ end of each mRNA. Hence, 5′ ends of trypanosomatid mRNAs are determined by the splice site, not the transcription initiation site. Accordingly, a specific RNA pol II initiation site has not been determined for protein coding gene transcription, suggesting that these parasites recruit RNA pol II to the DNA by a novel mechanism which is independent of general transcription factors.

However, trypanosomatids utilize RNA pol II also for the synthesis of SL RNA and, in contrast to the protein coding genes, SL RNA genes (SLRNAs) are transcribed monocistronically from a concrete transcription initiation site (18,19). SL RNA synthesis is of crucial importance to the parasites because maturation of each mRNA results in the consumption of one SL RNA molecule. To accommodate a high synthesis rate, SL RNA is encoded in ~100 tandemly arranged SLRNAs per haploid genome. The SLRNA promoter is conserved within trypanosomatids and consists of a bipartite upstream sequence element (USE) and an initiator element (2023). Recently, a transcription factor complex was characterized in T.brucei which specifically binds the USE, is essential for SLRNA transcription in vitro, and consists of TRF4, the small nuclear RNA activating complex (SNAPc) and a highly divergent TFIIA (24,25). In the human system, the same factors are involved in RNA pol II-mediated transcription of small nuclear RNA genes [reviewed in (26)]. However, this enzymatic activity also requires other general transcription factors including TFIIB (27). Hence, the parallels to the human system and the identification of TFIIA in T.brucei suggest that, in contrast to the predictions of the genome analyses, trypanosomatids do harbor a highly divergent set of general transcription factors. Here we report the identification of a trypanosomatid TFIIB-like (TFIIBlike) protein which, as would be anticipated for TFIIB, is an essential transcription factor of T.brucei SLRNA transcription interacting with RNA pol II.

MATERIALS AND METHODS

Plasmids

The transfection vector pTFIIBlike-RNAi is a derivative of the T.brucei stem–loop RNAi vector (28,29) containing the TFIIBlike coding region from position 534 to 1008 as inverted repeats around a stuffer sequence. pTFIIBlike-PTP-NEO was derived from pC-PTP-NEO (30) by fusing 827 bp of the C-terminal TFIIBlike coding sequence to the PTP sequence via restriction sites ApaI and NotI. For the dot blot analysis, pTZ18U-derived plasmids were generated in which the complete coding regions of SL RNA, GPEET procyclin, α tubulin, HSP70, 18 S rRNA, U2 snRNA or U6 snRNA were cloned into the SmaI site of the polylinker.

Cells

Procyclic cell culture, targeted integration of linear DNAs into cells by electroporation, and the generation of stable cell lines by selection and limiting dilution were described in detail previously (3133). For TFIIBlike silencing, T.brucei 29.13.6 cells (34) were transfected with 10 µg of the SacII-linearized construct pTFIIBlike-RNAi and cloned by limited dilution in the presence of 50 µg/ml of hygromycin, 15 µg/ml of G418 and 2.5 µg/ml of phleomycin. Silencing of TFIIBlike expression was induced by adding doxycycline to the culture medium at a final concentration of 10 µg/ml. Cells were counted daily and diluted to 2 × 106 cells/ml. The clonal cell line TbH8 which exclusively expressed PTP-tagged TFIIBlike was generated in a first step by integrating StuI-linearized plasmid TFIIBlike-PTP-NEO into one TFIIBlike allele. In a second step, the remaining TFIIBlike allele was replaced by a DNA amplification product of the hygromycin phosphotransferase coding region which was flanked by 100 nt of TFIIBlike untranslated region on either side. TbH8 cells were resistant to 40 µg/ml of G418 and 20 µg/ml of hygromycin. For each cell line, correct construct integration was confirmed by PCR and Southern analysis.

RNA analysis

For steady state analysis of SL RNA and U snRNA abundance in cells in which TFIIBlike silencing had been induced, total RNA was prepared from induced and non-induced cells and assayed by primer extension using the 5′-32P-end labeled oligonucleotides SLf and U2f (35), and the Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol. For nascent RNA labeling, cells were permeabilized with the detergent lysolecithin as detailed elsewhere (36), mixed into a transcription cocktail containing 20 mM potassium l-glutamate, 20 mM HEPES–KOH, pH 7.7, 3 mM MgCl2, 1 mM DTT, 25 mM creatine phosphate, 0.6 mg/ml creatine kinase, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 2 mM ATP, 1 mM GTP, 1 mM CTP, 5 µM UTP and 100 µCi of [α-32P]UTP (3000 Ci/mmole); and incubated for 8 min at 28°C. A small aliquot of total RNA prepared from these cells was separated on 6% polyacrylamide–50% urea gels and visualized by autoradiography. For dot blot assays, denatured plasmid DNA (5 µg per dot) was blotted onto positively charged nylon membrane and hybridized to the remainder of the radio-labeled RNA for 16 h at 55°C. Blots were then washed twice with 2× SSC/0.1% SDS for 5 min at room temperature and twice with 0.2× SSC/0.1% SDS for 15 min at 65°C. Signals were visualized by autoradiography and quantified by scintillation counting.

Immunofluorescence

Cells were harvested, washed once with phosphate-buffered saline (PBS, pH 7.4), and resuspended in 60 µl of PBS. Eight microliters of the cell suspension were fixed onto a glass slide overnight in methanol at −20°C. The slides were incubated with rat anti-TFIIBlike polyclonal serum, or the corresponding pre-immune serum diluted 1:100 in PBS/1% BSA. After washing with PBS containing 0.1% Tween 20 (Sigma, St Louis, MO), 4,6-diamidino-2-phenylindol (DAPI, 2 µg/ml) and a 1:400 dilution of the secondary anti-rat IgG conjugated with fluorescein isothiocyanate (FITC) were added. The rinsed samples were mounted in Vectashield (Vector laboratories); images were made using a Zeiss Axiovert 200 microscope with identical exposure settings and analyzed with AxioVision software.

Protein analysis

TFIIBlike-PTP was purified from crude TbH8 extract by IgG affinity chromatography, TEV protease treatment and anti-ProtC immunoaffinity chromatography exactly as described previously (30). Purified proteins were separated on a 10–20% SDS–polyacrylamide gradient gel and stained with Pierce Gelcode Coomassie stain (Pierce, Rockford, IL). Protein bands were excised and analyzed by MALDI-TOF mass spectrometry. For the expression and purification of TFIIBlike in Escherichia coli, the complete coding sequence of this protein was C-terminally fused to ProtC, a thrombin cleavage site and the 6× His tag in the expression vector pET100/D-TOPO (Invitrogen). The recombinant protein was expressed in BL21Star (DE3) cells upon induction with 1 mM IPTG at 37°C for 4 h. rTFIIBlike was purified by TALON metal affinity chromatography (BD Biosciences), thrombin cleavage and anti-ProtC immunoaffinity chromatography. For functional analysis, rTFIIBlike was directly eluted into transcription buffer in the presence of 0.5 mg/ml of ProtC peptide. Monospecific polyclonal antiserum directed against TFIIBlike was generated by hyper-immunizing female Sprague Dawley rats with purified rTFIIBlike protein mixed 1:1 with Freunds complete adjuvant (Sigma) which was administered intra-peritoneally. The rats were boosted after 3 and 6 weeks with the same antigen mixed 1:1 with Freunds incomplete adjuvant (Sigma). Pre-immune sera was collected from each animal prior to immunization to serve as controls. All animal handling and procedures were performed according to protocols approved by the University of Connecticut Animal Care Committee. For the bioinformatic analysis of the TFIIBlike zinc ribbon domain, the Superfamily server was used and human TFIIB protein sequence served as the query for the ten Hidden Markov models in the zinc beta-ribbon superfamily. The trypanosomatids sequences were then aligned against these models.

Functional in vitro analysis

Promoter pull-downs were carried out as described previously (37) except that the salt concentration in the binding reaction and in the washing buffers was increased to 80 mM potassium chloride. The in vitro transcription system has been detailed elsewhere (31,38). Briefly, reactions of 40 µl containing 8 µl of extract, 20 mM potassium l-glutamate, 20 mM KCl, 3 mM MgCl2, 20 mM HEPES–KOH, pH 7.7, 0.5 mM of each nucleoside triphosphate (NTP), 20 mM creatine phosphate, 0.48 mg/ml of creatine kinase, 2.5% polyethylene glycol, 0.2 mM EDTA, 0.5 mM EGTA, 4 mM DTT, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 12.5 µg/ml vector DNA, 20 µg/ml GPEET-trm template and 7.5 µg/ml SLins19 template were incubated for 1 h at 27°C. In addition, the ProtC peptide was added to a final concentration of 0.1 mg/ml in each reaction to eliminate a nonspecific effect in reconstitution assays performed with peptide-eluted protein. Specific transcripts from GPEET-trm and SLins19 were analyzed by extension of 32P-end-labeled primers Tag_PE (31) and SLtag (21) which hybridize to unrelated oligonucleotide tags of GPEET-trm and SLins19, respectively.

RESULTS AND DISCUSSION

Identification of a trypanosomatid TFIIBlike protein

Homology searches of trypanosomatid genome databanks with human or yeast TFIIB amino acid sequences yielded only one significant match to an ortholog of the TFIIB-related protein 1 (BRF1), a subunit of transcription factor TFIIIB involved in RNA pol III-mediated transcription initiation (data not shown). By analyzing all sequences from the homology search for the presence of one of the key features of the TFIIB family of proteins, namely, an N-terminal zinc binding motif, we identified a candidate TFIIB protein which we tentatively designated as TFIIBlike. The corresponding gene is present in each of the Tritryp parasites and has been annotated to encode a hypothetical conserved protein. The T.brucei TFIIBlike consists of 345 amino acids and has a predicted mass of 37.6 kDa and a theoretical pI of 6.28 (accession numbers: GeneDB Tb09.160.4220, cDNA AM235385). These attributes are similar to those of other eukaryotic TFIIBs (data not shown). All three trypanosomatid sequences contain the zinc-binding motif and conserved residues in most positions of the zinc ribbon beta strands (Figure 1A). Using the Superfamily Server (39), the trypanosomatid proteins were predicted to form a corresponding beta sheet in this domain (data not shown). In TFIIB/TFB, the B finger is located adjacent to the zinc ribbon. The trypanosomatid sequences contain a highly conserved sequence element at this position, but it deviates substantially from the B finger consensus of other organisms. However, the B finger key residues (Glu51 and Arg66 in the human sequence), which form a salt bridge and are essential for the integrity of this structure, are conserved in the Tritryp parasites (Figure 1A) [Refs (6,40)]. Moreover, in the TFIIB core domain, the trypanosomatid sequences exhibit many conserved residues within the first of the two cyclin repeats (Figure 1A). On the other hand, cyclin repeat 2 is much less well conserved and most of the mapped contact residues of human TFIIB with TBP and promoter DNA (7) are absent in the trypanosomatid sequences (Figure 1A and data not shown).

Figure 1
Trypanosomatid TFIIBlike is highly divergent to eukaryotic TFIIB and archaeal TFB. (A) Conserved TFIIB domains were aligned manually (B finger) or with ClustalW. TFIIB sequences are from human (hu, accession number AAH21000), Drosophila melanogaster (Dm, ...

Phylogenetic analysis also suggested that the trypanosomatid sequences are more divergent than their putative eukaryotic and archaeal counterparts (Figure 1B). This result was not due to insertions or C-terminal extensions present in the trypanosomatid TFIIBlike sequences because their exclusion from the analysis did not significantly change the phylogenetic tree (data not shown). The accuracy of the tree is further supported by the correct clustering of archaeal, trypanosomatid, fungal and metazoan species. Taken together, these data suggest that trypanosomatid parasites harbor a highly divergent member of the TFIIB family of proteins.

Silencing of T.brucei TFIIBlike is lethal and affects the synthesis of SL RNA

To evaluate whether TFIIBlike is a factor of SLRNA transcription, we silenced T.brucei TFIIBlike expression in vivo by conditional RNAi. For this, we employed the tetracycline-inducible RNAi system in T.brucei which involves the stable transfection of a construct for double-stranded RNA (dsRNA) synthesis under the control of tetracycline repressor into trypanosomes constitutively expressing this bacterial repressor protein (34). After transfection of a stem-loop vector (28,29) harboring both strands of the TFIIBlike coding region from position 534 to 1008, three clonal cell lines were obtained and raised by limiting dilution. In the absence of antibiotic, the cell growth in those lines was logarithmic. However, when TFIIBlike dsRNA synthesis was induced by adding doxycycline to the medium, cells began to die 48 h after induction, leading to a cessation of cell growth (Figure 2A). The partial growth recovery beginning at day six is a phenotype commonly observed in trypanosomes and attributed to the emergence of RNAi-defective cells (41). Northern blot analysis confirmed that TFIIBlike mRNA was quantitatively degraded within 48 h after induction of TFIIBlike dsRNA synthesis (Figure 2B). Similarly, immunodetection of TFIIBlike protein with a polyclonal antibody raised against the purified recombinant protein showed that only trace amounts of TFIIBlike were detectable 48 and 54 h after induction (Figure 2C). We concluded, therefore, that TFIIBlike is essential in trypanosomes.

Figure 2
T.brucei TFIIBlike is an essential gene. (A) Growth curve of a clonal procyclic T.brucei cell line in which the addition of doxycycline induces the expression of TFIIBlike dsRNA. Cells were diluted to a density of 2 × 106 cells/ml each day and ...

Since TFIIBlike may be involved in SL RNA synthesis, we first determined the abundance of SL RNA in total RNA preparations from cells in which TFIIBlike expression was silenced. A primer extension assay revealed that, in comparison to the RNA pol III-transcribed U2 snRNA, the amount of SL RNA was dramatically reduced 48 h after the addition of doxycycline (Figure 3A). To analyze whether this reduction was caused by a defect in synthesis, we labeled nascent RNA using a permeabilized cell system (36) in which newly synthesized SL RNA can be directly visualized when labeled, nascent RNA is separated by denaturing PAGE (21,36). As shown in Figure 3B, silencing of TFIIBlike did not significantly affect labeling of either tRNA or high molecular weight RNA within the 54 h time frame of the experiment, whereas the level of labeled SL RNA was reproducibly decreased 2- to 3-fold after 48 h of induction with doxycycline. The labeled RNA was also used in a dot blot analysis to assess the effect of TFIIBlike silencing on specific genes (Figure 3C and D). These experiments confirmed that silencing of TFIIBlike reduced SL RNA synthesis nearly 3-fold 54 h after induction. In contrast, transcription of the U2 and U6 snRNA genes, as well as RNA pol I-mediated synthesis of 18 S rRNA, was not greatly affected within this same time frame. The RNA pol I of T.brucei is unique since it transcribes not only the large ribosomal gene unit (RRNA), but also the gene units encoding the major cell surface antigens GPEET and EP procyclin and variant surface glycoprotein (VSG) (33). Interestingly, the dot blot analysis suggested that TFIIBlike silencing affected mRNA synthesis by RNA pol I and II differently. While labeling of GPEET procyclin mRNA was not greatly affected, RNA pol II–mediated synthesis of α-tubulin and HSP70 mRNA was significantly reduced 54 h after induction, albeit not as strongly as was transcription of SLRNA. Taken together, these data strongly suggested that TFIIBlike plays an important role in SLRNA transcription, and in addition, they raised the possibility that this protein may also function in RNA pol II-mediated polycistronic transcription of protein coding genes.

Figure 3
Silencing of TFIIBlike expression affects SL RNA synthesis in vivo. (A) Total RNAs prepared from RNAi-induced cells were analyzed by primer extension assays using 5′-32P-end labeled oligonucleotides specific for SL RNA and U2 snRNA. The lower ...

Interestingly, a recent study on archaean TFB revealed a stimulatory transcription effect of this factor which is recruitment-independent (42). Hence, it is possible that even if in trypanosomatids RNA pol II recruitment to protein coding genes is non-specific, TFIIBlike may enhance successful transcription initiation by interacting with the RNA polymerase.

TFIIBlike localizes to the nucleus and interacts with RNA pol II and the SLRNA promoter

As a transcription factor, TFIIBlike should predominantly localize to the nucleus. To test this, we used polyclonal antisera to detect TFIIBlike in fixed procyclic cells by indirect immunofluorescence. As expected, the antiserum exclusively stained the nucleus whereas there was no labeling with the pre-immune serum (Figure 4).

Figure 4
TFIIBlike is localized to the nucleus. Wild-type cells were fixed and stained with DAPI. TFIIBlike was detected using either a polyclonal antisera or, as a control, with the corresponding pre-immune serum and a FITC-labeled anti-rat IgG secondary antibody. ...

For a functional in vitro analysis, we generated the procyclic cell line TbH8 which exclusively expressed TFIIBlike C-terminally fused to the PTP tag (TFIIBlike-PTP). The PTP tag is an epitope combination for tandem affinity purification and consists of two protein A moieties, a tobacco etch virus protease site and the protein C epitope (30). Exclusive expression of TFIIBlike-PTP in TbH8 cells was achieved by replacing one allele of the single copy TFIIBlike gene with the hygromycin phosphotransferase gene and by targeted integration of construct pTFIIBlike-PTP-NEO into the second allele (Figure 5A). Immunoblot analysis of extracts derived from wild type and TbH8 cells using antibodies directed against the PTP tag or TFIIBlike showed that TbH8 cells expressed TFIIBlike-PTP but not the untagged protein (Figure 5B). Since TFIIBlike is an essential gene and TbH8 cells did not exhibit a growth defect (data not shown), we inferred that the C-terminal tag did not critically interfere with TFIIBlike function. We first used this cell line to purify TFIIBlike-PTP from crude TbH8 extract (Figure 5C). The final eluate contained several proteins with sizes ranging from 200 to 10 kDa. Most of these proteins were present in approximately stoichiometric amounts and the apparent sizes of the top band doublet and of the band beneath correlated well with the predicted molecular masses of the two largest RNA pol II subunits RPB1 (197 kDa) and RPB2 (135 kDa). Excision of these bands and mass spectrometric identification of the proteins confirmed that both bands of the doublet contained RPB1 and that the lower band consisted of RPB2 (data not shown). Hence, TFIIBlike interacts with RNA pol II in a manner which is unaffected by tandem affinity purification.

Figure 5
PTP purification of TFIIBlike. (A) Illustration of the two TFIIBlike alleles in the clonal cell line TbH8. In one allele, the TFIIBlike coding region was replaced by the hygromycin phosphotransferase gene (HYG-R) and in the second allele, the PTP coding ...

To assess whether TFIIBlike binds to the SLRNA promoter, we employed a promoter pull-down assay using the TbH8 cell line and linear promoter DNA fragments which comprised complete promoter regions to allow the formation of a stable pre-initiation complex. The promoter DNAs were labeled with biotin, immobilized on streptavidin beads, and incubated with transcriptionally active cell extract. The proteins which remained bound to the DNAs after several washing steps were analyzed by immunoblotting (Figure 6). In comparison to pull-downs with unrelated control DNA, TFIIBlike–PTP clearly interacted with the SLRNA promoter but not with the class I GPEET, RRNA and VSG expression site promoters (Figure 6, compare lane 2 with lanes 3, 4, 6 and 8). In addition to the proximal RRNA promoter (RRNA −84/+126) which is sufficient for maximal transcription in vitro (31), we analyzed the promoter fragment RRNA −257/−162 which harbors the most distal domain of the RRNA promoter. The relevant sequence elements of this domain resemble an oppositely oriented SLRNA USE and bind SNAPc and TRF4 (37,38) (data not shown) but not TFIIBlike (Figure 6, lane 5). This result is in accordance with the finding that the region upstream of the RRNA promoter is transcriptionally silent (37). In addition to these characterized promoters, we analyzed the intergenic region of HSP70 genes 2 and 3 (H23) which was identified previously as a potential promoter region for RNA pol II-mediated transcription of protein coding genes (43). A pull-down assay performed using H23 DNA reproducibly yielded a signal slightly above background (Figure 6, lane 7). Given that this DNA fragment was the longest fragment used in these analyses, it is likely that the larger DNA length caused an increased background signal. Collectively, our data suggest that TFIIBlike specifically binds to the SLRNA promoter, but does not recognize either class I promoters or the H23 intergenic region.

Figure 6
TFIIBlike specifically binds to the SLRNA promoter. Promoter pull-down assays were carried out with TbH8 extract and immobilized DNAs of GPEET, SLRNA, RRNA and VSG expression site (VSG ES) promoters. The numbers indicate the end positions of the DNA fragments ...

TFIIBlike is essential for SLRNA transcription in vitro

Finally, we investigated the function of TFIIBlike in an in vitro system in which SLRNA and RNA pol I-mediated transcription are active (31). We used the TbH8 cells because the high affinity interaction of the protein A domains within the PTP tag with immobilized IgG allowed efficient and rapid depletion of TFIIBlike-PTP from the extract (Figure 7A). Reactions were carried out in which the SLRNA promoter template SLins19 and, as a control, the class I GPEET promoter template GPEET-trm were co-transcribed. Both of these constructs harbor unrelated sequence tags downstream of the transcription initiation site which allow for specific detection of the corresponding RNAs by primer extension analysis. As shown in Figure 7B, depletion of TFIIBlike completely abolished SLRNA transcription without affecting GPEET promoter transcription (lane 3), while a mock depletion had only a slight effect on the transcription on both templates (compare lanes 1 and 2). This result is in close agreement with our earlier finding that TFIIBlike did not bind to the GPEET promoter in the pull-down assay. To confirm that TFIIBlike was responsible for the effect on SLRNA transcription, we attempted to reconstitute the transcriptional activity in the depleted extract by adding back purified protein. The final eluate of purified TFIIBlike-PTP (see above, Figure 5C) reconstituted SLRNA transcription in a dose-dependent manner (Figure 7B, lanes 6–8). A control eluate from the PTP-tagged and purified largest subunit of RNA pol I did not affect SLRNA transcription (lane 4), demonstrating that the TFIIBlike portion of the fusion protein was responsible for the restoration of activity. Similarly, the SNAP2-PTP purified TRF4/SNAPc/TFIIA complex which has been shown to be functional, did not reconstitute SLRNA transcription in the TFIIB-depleted extract (lane 5). This result also indicates that the SNAP2-P eluate did not contain TFIIBlike and that a putative interaction of TRF4 and TFIIBlike is not resistant to the conditions of tandem affinity purification. While these experiments strongly suggested that TFIIBlike is essential for SLRNA transcription, it was still possible that RNA pol II or other proteins that co-purified with PTP-tagged TFIIBlike were responsible for the observed transcriptional effects. To unequivocally demonstrate that these effects can be attributed to TFIIBlike alone, we expressed and purified the protein in E.coli. When added back to TFIIBlike-depleted extract, recombinant TFIIBlike was as efficient in reconstituting SLRNA transcription as was the eluate of the tandem affinity purification (Figure 7B, lanes 9–11). Hence, we concluded that TFIIBlike is indispensable for SLRNA transcription. Since this protein was able to reconstitute SLRNA transcription in an extract with no detectable SLRNA transcription activity, we infer that TFIIBlike is a basal transcription factor.

Figure 7
SLRNA transcription in vitro essentially depends on TFIIBlike. (A) Transcriptionally active extract of TbH8 cells was depleted of TFIIBlike-PTP by IgG affinity chromatography. Mock-treated and depleted (depl) extracts were analyzed by immunoblotting using ...

Although it will require a more detailed characterization to determine whether TFIIBlike is the ortholog of TFIIB/TFB, our results indicate that this is indeed the case. Why then has TFIIBlike diverged much more than trypanosomatid BRF1? Both factors are functionally similar and bridge promoter bound factors with RNA pol II and III, respectively. A likely explanation is that while trypanosomatids harbor a standard set of small RNA genes which are transcribed by RNA pol III and most likely require BRF1 for transcription initiation, a class II pre-initiation complex is formed at relatively few sites in the genome thus reducing the constraints on TFIIBlike conservation. It is possible that TFIIBlike interacts only with the SLRNA promoter but even if this factor is involved in recruiting RNA pol II to protein coding genes, initiation sites may be limited due to the polycistronic mode of transcription. Accordingly, strand-specific nuclear run-on assays in L.major suggested that protein coding gene transcription initiates predominantly at strand-switch regions between divergent gene clusters (17).

CONCLUSION

We have identified a TFIIBlike protein in trypanosomatid parasites and our functional analysis suggests that this protein is the orthologue of eukaryotic TFIIB and archaeal TFB. If this is the case, further analysis of TFIIBlike function may resolve the long standing question on how RNA pol II is recruited to protein coding genes in trypanosomes. Most importantly, however, is our finding that TFIIBlike is absolutely required for SL RNA synthesis in T.brucei. Since this process is vital to all life cycle stages of trypanosomatid parasites and since the protein is so divergent to other members of the TFIIB family of proteins, a detailed functional and structural characterization of TFIIBlike and its interactions with other proteins may reveal a parasite-specific target in a fundamentally important process.

Acknowledgments

We thank Mary Lee (New York University) for providing HSP70 constructs, Christian Tschudi (Yale University) for discussion, and Justin Radolf (UCHC) for critically reading the manuscript. The trypanosomatid genomes were sequenced at TIGR and the Sanger Centre with support from the NIH and the Wellcome Trust. This work was supported by grants of the National Institute of Health (AI059377 to A.G. and AI-29735 to M.J.C.) and an institutional fund of the University of Connecticut Health Center to A.G. Funding to pay the Open Access publication charges for this article was provided by National Institute of Allergy and Infectious Disease grant AI059377.

Conflict of interest statement. None declared.

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