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From:
doi: 10.1128/MMBR.70.1.157-176.2006
TABLE 1.
Linkage type distribution in glucans synthesized by GS enzymes from LAB produced in recombinant hosts
Glucan and strainGlucan linkage type distributionbMethod usedaGlucan size (Da)GeneProtein size (residues)Reference(s)
ReuteranTerminalα-(1→4)α-(1→6)α-(1→4,6)
    L. reuteri 12111462617Met/1H80 × 106gtfA1,78197
    L. reuteri ATCC 557309691113Met/1H45 × 106gtfO1,78194
DextranTerminalα-(1→3)α-(1→6)α-(1→3,6)
    L. reuteri 18010265113Metgtf1801,77295
    L. sakei Kg1549869MetgtfKg151,56195
    L. fermentum Kg337897MetgtfKg31,59595
    L. parabuchneri 3369759Metgtf331,46395
    L. mesenteroides NRRL B-1299158513CdsrA1,290125
    L. mesenteroides NRRL B-129959513CdsrB1,508118
    L. mesenteroides NRRL B-512F59513CdsrS1,527119, 156
    L. mesenteroides NRRL B-512F405013CdsrT51,49955, 56
    L. mesenteroides Lcc4Mainly13CdsrD1,527135
    S. mutans GS-5157015MetgtfD1,43069, 76, 173
    S. downei MFe289013CgtfS1,36561
    S. sobrinus OMZ176616735MetgtfT1,46868
    S. salivarius ATCC 2597510013CgtfK1,599179
    S. salivarius ATCC 259759513CgtfM1,577179
    S. gordonii CH1257513CgtfG1,57764, 214, 215
MutanTerminalα-(1→3)α-(1→6)α-(1→3,6)
    L. reuteri ML118472613MetgtfML11,77295
    S. mutans GS-58875MetgtfB1,47554, 175
    S. mutans GS-58687MetgtfC1,37554, 203
    S. downei MFe280.58820.513CgtfI1,597163
    S. sobrinus 6715Mainly13CgtfIa1,59290
    S. salivarius ATCC 259759013CgtfJ1,517179
GlucanTerminalα-(1→6)α-(1→3,6)
    S. sobrinus B13N44253113CgtfU1,55467
    L. mesenteroides NRRL B-1355Alternating α-(1→3) and α-(1→6) linkages (alternan)
Met/13Casr2,0574, 32
    L. mesenteroides NRRL B-1299α-(1→2,6) and α-(1→3,6) linkages
Met/13CdsrE2,83517, 170
    S. salivarius ATCC 25975Equal numbers of α-(1→3) and α-(1→6) linkages13CgtfL1,449179
aIndicates method used in linkage type analysis: Met, methylation; 13C, 13C-NMR; 1H, 1H-NMR.
bBoldface type indicates main linkage.
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