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Appl Environ Microbiol. 1996 January; 62(1): 266–268.
PMCID: PMC1388757

Optimal Renewal Rate and Nutrient Concentration for the Production of the Marine Microalga Phaeodactylum tricornutum in Semicontinuous Cultures

Abstract

A factorial experiment comprising six nutrient concentrations and six renewal rates was set to optimize semicontinuous cultures of Phaeodactylum tricornutum. Maximal cell density, 85 x 10(sup6) cells ml(sup-1), was obtained with 8 and 16 mmol of N per liter and a renewal rate of 10%. Protein stabilized at about 60% of the organic fraction for nitrogen-sufficient cultures. Both lipids and carbohydrates were stored as energetic reserves.


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