PMCCPMCCPMCC

Conseils de recherche
Les critères de recherche 

Avancée

 
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
 
Appl Environ Microbiol. 1995 January; 61(1): 310–316.
PMCID: PMC1388333

Occurrence of Arginine Deiminase Pathway Enzymes in Arginine Catabolism by Wine Lactic Acid Bacteria

Abstract

l-Arginine, an amino acid found in significant quantities in grape juice and wine, is known to be catabolized by some wine lactic acid bacteria. The correlation between the occurrence of arginine deiminase pathway enzymes and the ability to catabolize arginine was examined in this study. The activities of the three arginine deiminase pathway enzymes, arginine deiminase, ornithine transcarbamylase, and carbamate kinase, were measured in cell extracts of 35 strains of wine lactic acid bacteria. These enzymes were present in all heterofermentative lactobacilli and most leuconostocs but were absent in all the homofermentative lactobacilli and pediococci examined. There was a good correlation among arginine degradation, formation of ammonia and citrulline, and the occurrence of arginine deiminase pathway enzymes. Urea was not detected during arginine degradation, suggesting that the catabolism of arginine did not proceed via the arginase-catalyzed reaction, as has been suggested in some earlier studies. Detection of ammonia with Nessler's reagent was shown to be a simple, rapid test to assess the ability of wine lactic acid bacteria to degrade arginine, although in media containing relatively high concentrations (>0.5%) of fructose, ammonia formation is inhibited.

Full Text

Le texte intégral de cet article est accessible comme PDF (186K).

Selected References

Ces références figurent dans PubMed. Il se peut que la bibliographie de l'article soit plus exhaustive.
  • Crane CJ, Abdelal AT. Regulation of carbamylphosphate synthesis in Serratia marcescens. J Bacteriol. 1980 Aug;143(2):588–593. [PMC free article] [PubMed]
  • Crow VL, Thomas TD. Arginine metabolism in lactic streptococci. J Bacteriol. 1982 Jun;150(3):1024–1032. [PMC free article] [PubMed]
  • Davis CR, Wibowo DJ, Lee TH, Fleet GH. Growth and Metabolism of Lactic Acid Bacteria during and after Malolactic Fermentation of Wines at Different pH. Appl Environ Microbiol. 1986 Mar;51(3):539–545. [PMC free article] [PubMed]
  • DEIBEL RH, NIVEN CF, Jr, WILSON GD. Microbiology of meat curing. III. Some microbiological and related technological aspects in the manufacture of fermented sausages. Appl Microbiol. 1961 Mar;9:156–161. [PMC free article] [PubMed]
  • DEIBEL RH, WILSON GD, NIVEN CF., Jr Microbiology of meat curing. IV. A lyophilized Pediococcus cerevisiae starter culture for fermented sausage. Appl Microbiol. 1961 May;9:239–243. [PMC free article] [PubMed]
  • Garvie EI. Leuconostoc oenos sp.nov. J Gen Microbiol. 1967 Sep;48(3):431–438. [PubMed]
  • GILBOE DD, WILLIAMS JN., Jr Evaluation of the Sakaguchi reaction for quanitative determination of arginine. Proc Soc Exp Biol Med. 1956 Apr;91(4):535–536. [PubMed]
  • Henick-Kling T, Sandine WE, Heatherbell DA. Evaluation of Malolactic Bacteria Isolated from Oregon Wines. Appl Environ Microbiol. 1989 Aug;55(8):2010–2016. [PMC free article] [PubMed]
  • Hiraoka BY, Mogi M, Fukasawa K, Harada M. Coordinate repression of arginine aminopeptidase and three enzymes of the arginine deiminase pathway in Streptococcus mitis. Biochem Int. 1986 Jun;12(6):881–887. [PubMed]
  • Izuagbe YS, Dohman TP, Sandine WE, Heatherbell DA. Characterization of Leuconostoc oenos Isolated from Oregon Wines. Appl Environ Microbiol. 1985 Sep;50(3):680–684. [PMC free article] [PubMed]
  • Montel MC, Champomier MC. Arginine catabolism in Lactobacillus sake isolated from meat. Appl Environ Microbiol. 1987 Nov;53(11):2683–2685. [PMC free article] [PubMed]
  • Ough CS. Ethylcarbamate in fermented beverages and foods. I. Naturally occurring ethylcarbamate. J Agric Food Chem. 1976 Mar-Apr;24(2):323–328. [PubMed]
  • Pilone GJ, Kunkee RE, Webb AD. Chemical characterization of wines fermented with various malo-lactic bacteria. Appl Microbiol. 1966 Jul;14(4):608–615. [PMC free article] [PubMed]
  • Sedmak JJ, Grossberg SE. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. Anal Biochem. 1977 May 1;79(1-2):544–552. [PubMed]
  • THORNE KJ, JONES ME. CARBAMYL AND ACETYL PHOSPHOKINASE ACTIVITIES OF STREPTOCOCCUS FAECALIS AND ESCHERICHIA COLI. J Biol Chem. 1963 Sep;238:2992–2998. [PubMed]
  • Weiller HG, Radler F. Uber den Aminosäurestoffwechsel von Milchsäurebakterien aus Wein. Z Lebensm Unters Forsch. 1976;161(3):259–266. [PubMed]
  • YASHPHE J, GORINI L. PHOSPHORYLATION OF CARBAMATE IN VIVO AND IN VITRO. J Biol Chem. 1965 Apr;240:1681–1686. [PubMed]

Les articles de Applied and Environmental Microbiology ont été offerts à titre gracieux par American Society for Microbiology (ASM).