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Logo of compmedJournal Web siteSee also archive of Canadian Journal of Comparative MedicineNow Published as Canadian Journal of Veterinary ResearchFormerly published as Canadian Journal of Comparative Medicine and Veterinary Science. 1940 to 1968.
 
Can J Comp Med. 1973 October; 37(4): 405–408.
PMCID: PMC1319799

Differentiation Among Strains of Goat Mycoplasma by Incident Light Immunofluorescence

Abstract

Antigenic differentiation between strains of goat mycoplasma was studied by direct fluorescent antibody reactions employing incident (vertical) ultraviolet light. Agar colonies of the mycoplasma grown in petri dishes were fixed by alcohol in situ, and stained with conjugated globulin before examination with ultraviolet light.

The fluorescent antibody (FA) conjugate against Vom strain of Mycoplasma mycoides var. capri was Vom strain-specific, no cross reaction with Mexico, Connecticut, or Maryland strains. Similarly, the Mexico strain conjugate was specific for colonies of Mexico, and did not cross with the Vom, strain. Additionally, the conjugate of the PG-2 strain of Mycoplasma agalactiae, which was specific for the colonies of PG-2 was refractory for the strain #99 of M. agalactiae.

It was therefore possible to utilize an immunofluorescent technique (incident ultraviolet light) to demonstrate differences among strains of M. mycoides var. capri and M. agalactiae.

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Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association