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We previously reported that human CD4+ Tregs secrete high levels of IL-10 when stimulated in the presence of dexamethasone and calcitriol (vitamin D3). We now show that following stimulation by allergen, IL-10–secreting Tregs inhibit cytokine secretion by allergen-specific Th2 cells in an IL-10–dependent manner. A proportion of patients with severe asthma fail to demonstrate clinical improvement upon glucocorticoid therapy, and their asthma is characterized as glucocorticoid resistant (SR, abbreviation derived from “steroid resistant”). Dexamethasone does not enhance secretion of IL-10 by their CD4+ T cells. Addition of vitamin D3 with dexamethasone to cultures of SR CD4+ T cells enhanced IL-10 synthesis to levels observed in cells from glucocorticoid-sensitive patients cultured with dexamethasone alone. Furthermore, pretreatment with IL-10 fully restored IL-10 synthesis in these cells in response to dexamethasone. Vitamin D3 significantly overcame the inhibition of glucocorticoid-receptor expression by dexamethasone while IL-10 upregulated glucocorticoid-receptor expression by CD4+ T cells, suggesting potential mechanisms whereby these treatments may overcome poor glucocorticoid responsiveness. We show here that administration of vitamin D3 to healthy individuals and SR asthmatic patients enhanced subsequent responsiveness to dexamethasone for induction of IL-10. This strongly suggests that vitamin D3 could potentially increase the therapeutic response to glucocorticoids in SR patients.
CD4+ Th2 cells are implicated in the pathogenesis of asthma and allergic diseases through the actions of IL-4 and IL-13, which switch B cells to IgE synthesis; IL-5, which plays a role in eosinophil maturation and survival; and IL-13, which regulates airway hyperresponsiveness and mucus hyperplasia (1). Glucocorticoids are the first-line antiinflammatory treatment for asthma (2). Their multiple inhibitory properties, including the inhibition of Th2 cytokine synthesis, are likely to contribute to clinical efficacy. Glucocorticoids also enhance IL-10 production in vitro by human CD4+ and CD8+ T cells (3), and glucocorticoid treatment induces the synthesis of IL-10 by airway cells in asthmatic patients (4). IL-10 is a potent antiinflammatory and immunosuppressive cytokine that mediates its major immunosuppressive function by inhibiting APC function and cytokine production by macrophages and dendritic cells, leading to profound inhibition of Th1 cell–mediated immunity (5). IL-10 also regulates effector responses associated with established allergic and asthmatic disease, including inhibition of cytokine production by Th2 cells as well as mast cell and eosinophil function and modulation of IgG4:IgE ratios (5, 6). There appears to be an inverse correlation between IL-10 levels and the incidence and/or severity of asthmatic and allergic disease (7–10). Furthermore, studies in animal models of transfer of the IL-10 gene directly to the lung (11) or adoptive transfer of IL-10 transfected CD4+ T cells (12) demonstrate that IL-10 can block allergic airway inflammation (reviewed in ref. 6). These studies imply a central role for IL-10 in disease control and suggest that the induction of IL-10 synthesis may contribute to the clinical efficacy of glucocorticoids in allergy and asthma.
A proportion of asthmatic patients fails to benefit from oral glucocorticoid therapy and are thus denoted as having glucocorticoid-resistant (SR, derived from “steroid resistant”) or insensitive asthma (13). SR is associated with in vitro and in vivo alterations in cellular responses to exogenous glucocorticoids. We have previously demonstrated that CD4+ T cells from SR asthma patients fail to induce IL-10 synthesis following in vitro stimulation in the presence of dexamethasone as compared with their glucocorticoid-sensitive counterparts (SS, derived from “steroid sensitive”) (14), suggesting a link between induction of IL-10 synthesis and clinical efficacy of glucocorticoids. Resistance to the inhibitory effects of glucocorticoids can be induced in T cells in vitro, for example, by incubation with IL-2 and IL-4 (15). It may, therefore, be possible to modulate the function of SR CD4+ T cells in vitro and restore IL-10 synthesis.
One potential source of IL-10 is Tregs, which control the function of effector T cells, thereby preventing responsiveness to self antigens and regulating immune responses. The major Treg populations described to date include naturally occurring CD4+CD25+ Tregs and inducible IL-10 or TGF-β secreting Tregs (16–18). IL-10–secreting Tregs have been described following different regimens of antigenic administration both in vitro and in vivo (19–24). Our initial studies showed that glucocorticoids enhanced the production of IL-10 by polyclonally stimulated T cells and that these cells inhibited IFN-γ production by human CD4+ T cells in an IL-10–dependent manner (3). More recently, we demonstrated in both mouse and human that a combination of dexamethasone and calcitriol, the active form of vitamin D3, induced high numbers of IL-10–producing T cells that made negligible amounts of Th1 and Th2 cytokines (25). The inhibitory activity of these cells was demonstrated in vivo, where adoptive transfer of IL-10–secreting Tregs inhibited the development of murine experimental allergic encephalomyelitis (25); and in vitro, where they inhibited the proliferation of naive T cells (26). However, no data are yet available on the capacity of drug-induced IL-10–secreting Tregs to inhibit previously activated T cells, specifically Th2 cells, a major therapeutic target in allergic asthma. In vitro suppression by CD4+CD25+ T cells appears to be independent of IL-10 (27) whereas in vivo regulation by these cells is IL-10 dependent in some models (28–30). The transcription factor Foxp3 has recently been reported to be specifically expressed by CD4+CD25+ Tregs, controlling their development and function (31). To date, the relationship between naturally occurring CD4+CD25+ Tregs and IL-10–secreting Treg subsets is unclear.
In this study, we describe the in vitro inhibitory potential of human IL-10–secreting Tregs, derived by stimulation in the presence of vitamin D3 and dexamethasone, to inhibit cytokine production by allergen-specific Th2 cells associated with immune pathology in allergic asthma. We further demonstrate that impaired induction of IL-10 by glucocorticoids in T cells from SR patients can be reversed by vitamin D3 and IL-10, leading to levels of IL-10 production comparable to those observed in SS asthmatic patients, and examine the mechanisms by which this may occur.
One week of culture of CD4+ T cells with anti-CD3, APCs, IL-2, and IL-4 in the presence of dexamethasone and vitamin D3 promoted an IL-10–expressing phenotype and a reduction in Th1- and Th2-associated cytokines as well as GM-CSF, a key cytokine implicated in allergic airway inflammation (32) (6% IL-10–positive T cells in medium versus 26% IL-10–positive T cells in the presence of vitamin D3 plus dexamethasone; Figure Figure1A).1A). In the absence of IL-4, T cells stimulated in the presence of vitamin D3 and dexamethasone showed reduced cell survival (data not shown) but still demonstrated enhanced IL-10 synthesis (4% IL-10–positive T cells in medium versus 15% IL-10–positive T cells in presence of vitamin D3 plus dexamethasone).
To determine a possible relationship between naturally occurring CD4+CD25+ Tregs and antigen-stimulated IL-10–secreting Tregs, we compared the expression of the transcription factor Foxp3 and IL-10 using real-time PCR analysis. In vitro–derived IL-10–secreting Tregs harvested at the end of 14 days of culture expressed comparatively low amounts of Foxp3 mRNA compared with unstimulated CD4+CD25+ Tregs isolated directly ex vivo (Figure (Figure1B).1B). In contrast, IL-10–secreting Tregs expressed markedly higher amounts of mRNA encoding IL-10 than CD4+CD25+ Tregs, suggesting that these 2 regulatory populations may represent 2 distinct lineages (Figure (Figure1B).1B). Similarly, to permit a comparison of drug-induced IL-10–secreting Tregs with TGF-β–secreting Th3 cells, CD4+ T cells were cultured in the presence or absence of vitamin D3 and dexamethasone in serum-free media to facilitate analysis of TGF-β secretion. The drug treatment enhanced IL-10 production, as assessed by both ELISA and quantitative PCR analysis, but no increase in TGF-β was observed in the same cultures, suggesting drug-induced IL-10–secreting Tregs are distinct from Th3-type regulatory cells (Figure (Figure2,2, A and B).
To determine whether drug-induced IL-10–secreting Tregs manifest regulatory function for inhibition of CD4+ T cell proliferation, autologous CD4+ T cells were isolated and labeled with the fluorescent dye CFSE. Cell cycle progression of CFSE-labeled CD4+ T cells was analyzed on day 6 by flow cytometry after stimulation with anti-CD3 and APCs (Figure (Figure3B;3B; the ModFit-generated proliferation index of CFSE CD4+ T cells in the absence or presence of anti-CD3 was 1.1 versus 5.95). IL-10–secreting Tregs blocked the cell cycle progression of the freshly isolated CD4+ T cells at a ratio of 1 IL-10–secreting Treg to 4 CFSE CD4+ T cells (Figure (Figure3D;3D; proliferation index, 1.55). This inhibition of proliferation by IL-10–secreting Tregs was reversed by the addition of anti–IL-10R mAbs (Figure (Figure3F;3F; proliferation index, 5.12), in contrast to an isotype control, which showed little effect (Figure (Figure3E;3E; proliferation index, 2.03). As few as 1 IL-10–secreting Treg could ablate the proliferative response of 16 CFSE-labeled responder T cells (data not shown). T cell lines generated with IL-4 in the absence of drugs had little or no effect on cell cycle progression of the autologous freshly derived CFSE-CD4+ T cells (Figure (Figure3C;3C; proliferation index, 5.3).
Allergen-specific Th2 cells and IL-10–secreting Tregs were generated in parallel cultures using T cells from the same atopic donor. After two 7-day cycles of stimulation with cat allergen presented by autologous, irradiated T cell–depleted PBMC, the cells were stimulated alone or together at a ratio of 1 IL-10–secreting Treg to 4 Th2 cells with APCs and with or without allergen. IL-10–secreting Tregs inhibited IL-5 and IL-13 synthesis by allergen-stimulated Th2 cells almost to the levels of cytokine production detected in supernatants of unstimulated Th2 cells (Figure (Figure3G).3G). As predicted from independent studies demonstrating IL-10 synthesis by Th2 cells, addition of anti–IL-10R mAbs to cultures of cat allergen–stimulated Th2 cells enhanced IL-13 cytokine production. Furthermore, the marked inhibition of IL-13 production by Th2 cells observed in the presence of IL-10–secreting Tregs was completely overcome by the addition of anti–IL-10R mAbs but not by isotype-matched control antibodies (Figure (Figure33H).
In addition to their ability to inhibit Th2 responses, IL-10–secreting Tregs also inhibited IFN-γ production by T cells in anti-CD3–driven stimulation cultures. Again, this inhibition was reversed by an anti–IL-10 receptor antibody but not by a neutralizing anti–TGF-β antibody (Figure (Figure2C).2C). Anti–TGF-β also failed to reverse IL-10–secreting Treg–mediated inhibition of Th2 cytokine production in this anti-CD3–driven culture system (Figure (Figure2C).2C). These data further emphasize that IL-10–secreting Tregs represent a population distinct from previously described naturally occurring and Th3 Treg populations.
We previously demonstrated that CD4+ T cells from SR patients show a statistically significant reduction in their capacity to respond to dexamethasone for induction of IL-10 synthesis in comparison with SS patients (14). We therefore tested whether vitamin D3 could enhance IL-10 synthesis in the SR cultures stimulated with anti-CD3 and APCs. Mean data from 8 SR patient cell cultures is shown in Figure Figure4A.4A. The poor response of CD4+ T cells from SR patients to dexamethasone in producing IL-10 was greatly enhanced by the addition of Vitamin D3 to the cultures, restoring IL-10 levels to those seen in cultures of cells from SS patients stimulated with dexamethasone alone such that these 2 groups were not significantly different. However, under none of the experimental conditions tested was IL-10 synthesis in the SR patient cultures fully restored to the levels observed in CD4+ T cells derived from SS patients.
Since the initial development of murine IL-10–secreting Tregs in the presence of dexamethasone and vitamin D3 is IL-10 dependent (25), we investigated whether pretreatment with IL-10 could further enhance the capacity of SR CD4+ T cells to produce IL-10 when stimulated in the presence of dexamethasone. T cells were incubated overnight either in medium or with 2 ng/ml IL-10 and then washed prior to polyclonal stimulation in the presence of the indicated combinations of dexamethasone and vitamin D3 for 7 days. All culture groups were then restimulated for 48 hours with anti-CD3 and IL-2 in the absence of the drugs. Following overnight preculture in medium prior to stimulation with anti-CD3 and dexamethasone plus vitamin D3, a clear difference in IL-10 synthesis was still maintained between the SR and SS patient groups (P < 0.01; Figure Figure4B).4B). In contrast, overnight pulsing with IL-10 prior to stimulation in the presence of dexamethasone alone fully restored IL-10 synthesis by CD4+ T cells from SR patients to the levels seen in SS patients. In addition, IL-10 pretreatment and subsequent stimulation in the presence of dexamethasone plus vitamin D3 further enhanced levels of IL-10 synthesis in SR CD4+ T cell cultures. IL-10 pretreatment did not, however, significantly affect the production of IL-10 by CD4+ T cells from SS patients stimulated in the presence of the 2 drugs (Figure (Figure44B).
Since we have implicated deficient IL-10 production by T cells in the mechanism of clinical glucocorticoid resistance, we hypothesized that IL-10 may regulate responsiveness of T cells to glucocorticoids and therefore tested to determine whether IL-10 modulates the expression of the glucocorticoid receptor (GR). Western blotting studies of total cell lysates from CD4+ T cells from nonasthmatic healthy individuals demonstrated an IL-10 concentration–dependent induction of GRα expression in comparison with cells cultured in medium alone (Figure (Figure5A).5A). Induction was already detectable at 6 hours but most marked following 18 and 24 hours of culture with IL-10. GRβ expression was not detected in the T cell lysates (Figure (Figure5B)5B) although it could be detected in U293 cells transfected with cDNA encoding FLAG-tagged GRβ, indicating very low expression of GRβ in human peripheral CD4+ T cells.
We investigated whether vitamin D3 might also act to increase GR expression since topical administration of calcitriol to patients with psoriasis has been shown to increase IL-10 locally in the skin (33). Although this was not the case (Figure (Figure6A),6A), we observed, as independently reported in certain cell lines (34), that the culture of CD4+ T cells with glucocorticoids leads to a dose-dependent decrease in GRα (Figure (Figure6B).6B). Strikingly, vitamin D3 abrogated the profound downregulation of GRα caused by dexamethasone (Figure (Figure6C),6C), suggesting a potential mechanism whereby vitamin D3 may contribute to the efficacy of dexamethasone in promoting glucocorticoid-induced IL-10 synthesis.
In a proof of concept study, the effect of ingestion of vitamin D3 in SR asthmatic patients was investigated. Subjects were bled prior to vitamin D3 ingestion and on days 1, 3, and 7 after ingestion of 0.5 μg vitamin D3 daily. T cells were stimulated with anti-CD3 in the presence or absence of dexamethasone for 7 days, using methodology identical to that used in earlier experiments, to determine any differences in reactivity to dexamethasone. Cell pellets were prepared at this time for mRNA extraction, or cells were recultured in the absence of drugs and analyzed for changes in IL-10 protein at 48 hours. In all SR individuals tested, IL-10 expression by CD4+ T cells was enhanced following vitamin D3 ingestion as assessed by intracellular cytokine staining, ELISA, and quantitative PCR (Figure (Figure7).7). In 2 donors (SR1 and SR2), this enhancement was sustained on days 1 and 3 as well as day 7 in SR2 over a range of dexamethasone concentrations. In the third donor, SR3, enhancement was observed on day 3 alone. Similar protein data were observed in 4 healthy individuals tested in a pilot study (Supplemental Figure 1; supplemental material available online with this article; doi:10.1172/JCI21759DS1 http://dx.doi.org/10.1172/JCI21759DS1; RNA not assayed in this earlier study). These preliminary data provide encouraging evidence of the capacity of vitamin D3 to promote responsiveness to glucocorticoids for the induction of IL-10 synthesis.
The present study demonstrates that human IL-10–secreting Tregs induced ex vivo following stimulation in the presence of vitamin D3 and dexamethasone secrete high levels of IL-10 that is associated with a potent capacity to inhibit immune responses implicated in the pathogenesis of allergic and asthmatic disease. Specifically, allergen-induced cytokine production by Th2 cells was inhibited by allergen-induced IL-10–secreting Tregs in an IL-10–dependent manner. Furthermore, we demonstrated that by combining preincubation with IL-10 and addition of vitamin D3 to the T cell stimulation cultures, a defect in glucocorticoid-induced IL-10 production by CD4+ T cells from SR asthma patients was fully overcome. This may result from our findings that IL-10 increased GR expression by human CD4+ T cells while vitamin D3 overcame ligand-induced downregulation of GR. Strikingly, ingestion of vitamin D3 by SR asthmatic patients enhanced subsequent responsiveness to dexamethasone for the induction of IL-10 synthesis, highlighting the potential clinical relevance of these observations. IL-10–secreting Tregs may hold therapeutic potential for asthma and allergic diseases, and our findings offer potential novel strategies for reversing glucocorticoid resistance.
We have previously described the capacity of vitamin D3 and dexamethasone to induce IL-10–producing T cells from both murine and human CD4+ T cells (25) and show here that the human IL-10–producing Tregs exhibit regulatory activity in vitro. Importantly, human IL-10–secreting Tregs could suppress previously activated antigen-specific Th2 cells, the therapeutic target in allergy and asthma. This inhibition of cytokine secretion from allergen-specific Th2 cells by IL-10–secreting Tregs was IL-10 dependent in this system. Since these cultures included APCs, this IL-10–dependent suppression may have been mediated via the well established effects of IL-10 on APCs (5).
CD4+CD25+ Tregs suppress allergen-induced proliferation and Th2 cytokine production by peripheral CD4+ T cells independently of IL-10 secretion (35). Here, we compared human IL-10–secreting Tregs and CD4+CD25+ T cells and show that unlike IL-10–secreting Tregs, expression of IL-10 by CD4+CD25+ Tregs was minimal. Furthermore, unlike CD4+CD25+ Tregs, IL-10–secreting Tregs did not express high levels of mRNA encoding the forkhead winged helix transcription factor FoxP3. Thus, although FoxP3 appears to be important for the development and function of naturally occurring CD4+CD25+ T cells (31), in vitro–derived IL-10–secreting Tregs appear to have regulatory function despite low levels of Foxp3 expression. We further demonstrate that drug-induced IL-10–secreting Tregs are distinct for TGF-β synthesizing Th3 cells, as they show no increased synthesis of TGF-β, and neutralizing antibody to this cytokine does not modify their inhibitory function (17).
Although inhaled glucocorticoids are very effective for the treatment of most patients with asthma, an important subgroup of patients require oral glucocorticoids to control their disease at the risk of considerable side effects, and a subgroup of these patients fail to show clinical improvement even with high-dose glucocorticoid therapy (13). These patients represent an important health-care problem and, consequently, if the response to glucocorticoid treatment could be enhanced in such patients, this would represent a major therapeutic advance. Our data imply that induction of IL-10 synthesis may contribute to the clinical efficacy of glucocorticoid therapy in asthma since IL-10 plays an essential role in the functional activities of IL-10–secreting Tregs and patients who fail to respond clinically to glucocorticoids also fail to respond ex vivo to glucocorticoids for induction of IL-10 synthesis (14). We now present evidence that it is possible to reverse this defect. First, addition of vitamin D3 to T cell cultures polyclonally stimulated with anti-CD3 and dexamethasone enhanced IL-10 synthesis in the SR cultures, restoring them to levels observed in SS cultures stimulated in the presence of dexamethasone alone. Secondly, administration of vitamin D3 orally to SR patients and healthy donors modified subsequent responsiveness of their CD4+ T cells to glucocorticoids for induction of IL-10 synthesis in all individuals tested. Vitamin D3 is at present occasionally administered to severe asthmatic patients as part of a prophylactic regimen against glucocorticoid-induced osteoporosis (36), and our studies suggest an additional potential benefit of such treatment. These data with vitamin D3 establish an important precedent showing that it is possible to boost IL-10 synthesis in this important patient group. However, it may also be possible to achieve similar cooperative effects between glucocorticoids and other drugs for induction of IL-10–secreting Tregs. Indeed, our recent studies demonstrate a similar capacity upon combining glucocorticoids with long-acting β2-agonists to induce an IL-10–secreting Treg profile (37).
The mechanisms by which IL-10 and vitamin D3 enhance glucocorticoid-induced IL-10 synthesis were investigated. We hypothesized that IL-10 itself may be regulating responsiveness to glucocorticoids and now demonstrate a concentration-dependent effect of IL-10 on GRα expression by human CD4+ T cells. The possible functional significance of this observation is supported by an independent study that demonstrated that IL-10 increased the sensitivity of human monocytes to dexamethasone and increased the number of tritiated dexamethasone binding sites in U937 cells (38). The antibody used in our Western blotting detects both α and β spliced variants of GR, and these forms are readily distinguishable in Western blotting by virtue of their size difference. We consistently observed enhancement of GRα in IL-10–treated T cells whereas the GRβ variant remained undetectable by Western blot in agreement with several independent studies (ref. 39 and references therein). Two studies have used immunohistochemistry to detect GR expression in tissues, with both suggesting that less GRα is available in SR. One study demonstrated that glucocorticoid resistance in asthma is associated with elevated in vivo expression of GRβ relative to GRα in skin biopsies (40) while the second showed that airway T cells from SR patients showed increased GRβ expression in comparison with cells from glucocorticoid-sensitive asthmatics or control subjects (41). Notably, however, although elevated GRβ was also reported in blood T cells from this patient group, the levels were much lower than those observed in airway T cells. Thus GRβ is expressed at extremely low levels in normal cells but may be upregulated in SR asthma (40, 42). GRβ has been proposed to act as a dominant-negative form of GR operative at glucocorticoid-response elements (43). Thus, the upregulation of GRα observed by Western blot may alter the GRα to GRβ ratio and therefore partially restore responsiveness. In studies into the mechanism of vitamin D3 action, we observed that dexamethasone induces a dose-dependent and profound downregulation of GR in primary human CD4+ T cells. Strikingly, coincubation of CD4+ T cells with vitamin D3 and dexamethasone was observed to rescue GR expression and lead to a less severe decrease of GR expression in the CD4+ T cell population. We found no direct effect of vitamin D3 on GR expression by human CD4+ T cells. We propose that these observations may, in part, explain the capacity of vitamin D3 to promote glucocorticoid-induced IL-10 synthesis.
It will be important to determine whether the defect in glucocorticoid-induced IL-10 synthesis observed in some asthmatic patients is a common feature of other immune-mediated inflammatory diseases that exhibit clinical refractoriness to glucocorticoid therapy, e.g., inflammatory bowel disease and rheumatoid arthritis (44). If failure to induce IL-10 ex vivo also correlates with clinical refractoriness to glucocorticoid therapy in other inflammatory conditions, then analysis of IL-10 synthesis may ultimately prove useful in disease management. For example, it could act as a useful predictive tool in determining which patients will be unresponsive to glucocorticoid therapy so that alternative treatment regimens can be introduced sooner.
Both our own and independent studies suggest the capacity of both glucocorticoids and vitamin D3 to promote IL-10 synthesis in vivo. Other regimens, such as peptide therapy in murine models or allergen immunotherapy in allergic patients, may also induce IL-10–secreting Tregs (45–47). However, allergen immunotherapy carries a risk of severe allergic reactions or anaphylaxis (48), which precludes its use for severe asthma in current dose regimens, and peptide therapy for autoimmune diseases carries similar risks (49, 50). Combination with drug therapy might allow safer use of immunotherapy and promote the induction of IL-10–secreting Tregs. Other strategies have been adopted for investigating IL-10–secreting Tregs in vitro in animal models and support a therapeutic role of IL-10 synthesizing T cells in alleviating allergic symptoms (6, 51–53). For example, 1 recent study demonstrated that CD4+ T cells engineered to express IL-10 prevent Th2-driven airway hyperresponsiveness and inflammation (12). Finally, although 2 groups suggest that IL-10 may be required in the development of murine airway hyperresponsiveness (54, 55), these represent extremes of either transgenic overexpression or the complete absence of IL-10 in the airways and therefore do not represent physiological conditions. We would argue that the challenge for the future will be to harness these beneficial drug-mediated effects and induce allergen-specific IL-10 synthesis in a local and controlled manner at the time of allergen exposure (6).
In conclusion, we show that human IL-10–secreting Tregs are distinct from CD4+CD25+ T cells and TGF-β–secreting Th3 cells and show in vitro suppressive activity against allergen-specific Th2 cells. Furthermore, we show that both vitamin D3 and IL-10 can overcome the defect in IL-10 production by CD4+ T cells from SR asthma patients. Induction of antigen-specific IL-10–secreting Tregs is an appealing future therapeutic area for asthma and allergic diseases.
PBMCs were obtained from healthy individuals and patients attending the Asthma Clinic at Guy’s Hospital, London, United Kingdom. Asthma was defined by American Thoracic Society criteria as reversible obstruction (≥15%) of the airways (14). Glucocorticoid resistance (SR) was defined as the failure of forced expired volume in 1 second (FEV1) to improve by 15% or more from a baseline of 75% or less after 14 days of 40 mg/day oral prednisolone, and glucocorticoid sensitivity (SS) was defined as an improvement of 25% or more in FEV1 following an identical course of prednisolone treatment. PBMCs were obtained from 7 SR patients (2 female, 5 male; mean age ± SD, 54 ± 15; mean basal FEV1, 55% ± 20% of predicted; and range of prednisolone reversibility, 0–14%) and from 5 SS patients (2 female, 3 male; mean age, 50 ± 10; mean basal FEV1, 46% ± 24% of predicted; and range of prednisolone reversibility, 26% to > 100%). All patients were nonsmokers and using inhaled (400–1000 μg) glucocorticoids daily and β2-agonists as required. Patients taking oral glucocorticoids within 1 month of study were excluded. All donors signed a consent form approved by the Ethics Committee at Guy’s Hospital.
PBMCs were isolated as previously described (3). CD4+ T cells were purified by positive selection using Dynabeads (Dynal Biotech; typical purity, 99.5%). Samples containing CD4+ T cells plus APCs, unless otherwise specified, were prepared by negative depletion using CD8+ Dynabeads (3). CD4+CD25+ T cells were isolated as previously described (35).
We stimulated 1 × 106 cells/ml CD4+ T cells or CD4+ T cells plus APCs with 1 μg/ml plate-bound or soluble anti-CD3 (OKT-3), respectively, 50 U/ml IL-2 (EuroCetus), 10 ng/ml IL-4 (NBS), calcitriol (Vitamin D3; BIOMOL), and dexamethasone (Sigma-Aldrich) as indicated. Cells were restimulated with cross-linked anti-CD3 and the same stimuli as in the first culture (3, 25). Where indicated, cells were recultured after 7 days with cross-linked anti-CD3 and IL-2 alone and supernatants harvested at 48 hours for cytokine analysis.
To generate allergen-specific T cell lines, PBMCs were obtained from atopic volunteers. Atopy was defined as previously described (3). We cultured 2 × 106 cells/ml CD4 plus APCs with 10 μg/ml of allergen and 10 ng/ml IL-4. The Th2 group also received 5 μg/ml anti–IFN-γ and anti–IL-12 (both from BD Biosciences — Pharmingen), and the regulatory group received 10–7 M dexamethasone and 10–7 M vitamin D3. On day 7, cells were washed and recultured under the same initial culture conditions plus fresh allergen and irradiated APCs (T cell–depleted PBMCs; 300 Gy). T cell lines were also derived from healthy volunteers by stimulating CD4 and APCs with anti-CD3 and IL-2 in the presence of IL-4 (TIL-4) or IL-4, vitamin D3, and dexamethasone (IL-10–secreting Tregs).
On day 14, fresh autologous CD4+ T cells were purified and labeled with 2 μM CFSE (Invitrogen Corp.), and 2 × 105 cells were cocultured with 5 × 104 autologous irradiated APCs and 5 × 104 TIL-4 or IL-10–secreting Tregs as indicated. Cultures were stimulated with soluble anti-CD3, with or without 5 μg/ml anti–IL-10 receptor (3F9-2; DNAX), 5 μg/ml anti–TGF-β (DNAX), or 5 μg/ml isotype control rat IgG2a (R35-9S; BD Biosciences — Pharmingen) as indicated for 6 days. Propidium iodide (Sigma-Aldrich) was then added to exclude dead cells and 10,000 CD4-CFSE–positive cells analyzed using a FACSCaliber flow cytometer (BD). The proliferation index was generated using ModFit LT software version 3.0 (Verity Software House). Alternatively, supernatants were harvested on day 3 and assayed by ELISA for cytokine content. For analysis of allergen-specific regulatory function, 14-day Th2 and IL-10–secreting Treg lines were generated. Cocultures contained the indicated combinations of 1 × 105 Th2, 5 × 104 IL-10–secreting Tregs, and 1 × 105 autologous irradiated APCs, stimulated with 10 μg/ml allergen and, where indicated, 5 μg/ml anti–IL-10 receptor or 5 μg/ml isotype control rat IgG2a added. Supernatants were harvested on day 3 for analysis of cytokine content.
On day 7 or day 7 plus 48 hours of a further anti-CD3–induced stimulation, cells were restimulated for 4 hours with 0.25 ng/ml PMA (Sigma-Aldrich) and 25 ng/ml ionomycin (Calbiochem), with monensin (Sigma-Aldrich) added for the final 2 hours. Cells were washed, fixed, and permeabilized using Cytofix/Cytoperm kit (BD Biosciences — Pharmingen) and triple-stained with fluorescently labeled monoclonal antibodies to IL-10, IL-2, IL-4, IL-13, GM-CSF (all at 100 ng/sample), or IFN-γ (250 ng/sample) (BD Biosciences — Pharmingen) (3, 25). Staining with isotype control antibodies (BD Biosciences — Pharmingen) was performed in all experiments. Dead cells (7-aminoactinomycin D [7-AAD] positive; Sigma-Aldrich) were gated out and 10,000 live cells analyzed by flow cytometry.
IL-5, IL-10, IL-13, and IFN-γ were measured using ELISA and matched antibody pairs (BD Biosciences — Pharmingen), with reference to commercial standards (R&D Systems). The lower limit of detection for IFN-γ and IL-10 was 50 pg/ml, for IL-5, 100 pg/ml, and for IL-13, 100 pg/ml. When less than 100 μl supernatant was available, a human Th1/Th2 cytometric bead array kit (BD Biosciences — Pharmingen) was used. TGF-β1 was measured using the TGF-β1 Emax Immunoassay System (Promega). For assay of total active TGF-β1, samples were diluted 1:5, treated with 1 N HCl, then neutralized with 1 N NaOH.
The study was approved by the Research Ethics Committee of Guy’s Hospital and informed consent obtained from volunteers. Four healthy volunteers (1 female, 3 male; 36–48 years old) and 3 SR patients (1 female, 2 male; 50–60 years old) were given 0.5 μg/day (2 × 0.25 μg) oral calcitriol for 7 days. PBMCs were obtained at days 0 (before treatment) and days 1, 3, and 7. Donors were always bled between 9:30 am and 10:30 am to control for any influence of diurnal changes. CD4+ T lymphocytes plus APCs were stimulated for 7 days with 1 μg/ml plate-bound anti-CD3, 50 U/ml IL-2, and varying concentrations of dexamethasone. On day 7, cells were washed and restimulated with PMA-ionomycin for 4 hours (cell pellets kept for mRNA extraction) or cross-linked with anti-CD3 and IL-2 for a further 48 hours. Cells were harvested at 48 hours for intracellular staining for IL-10 and culture supernatants harvested for cytokine analysis.
CD4+ T cells were incubated with human recombinant IL-10 (R&D Systems), vitamin D3, and/or dexamethasone for the indicated periods (0 to 72 hours), then they were washed, soluble cell proteins were extracted, and Western blot was performed as previously described (56). GR was detected using a mouse monoclonal antibody (BD Transduction Laboratories; BD Biosciences — Pharmingen) and goat anti-mouse horseradish peroxidase–conjugated secondary antibody. Immunoreactive complexes were revealed by ECL (Amersham Biosciences) and visualized by autoradiography. Discrimination between GRα and GRβ was facilitated by the use of FLAG epitope–tagged GRα and GRβ cDNAs. An empty vector (pcDNA3; Invitrogen Corp.) was used as a negative control. Overexpression of these cDNAs in U293 cells permitted Western blotting with either anti-FLAG (Sigma-Aldrich; not shown) or anti-GR (56). The different migration of these 2 proteins and comparison with native protein allowed discrimination among spliced variants.
RNA was extracted from cell pellets using either the Absolutely RNA kit (Stratagene) or the RNeasy Mini kit (QIAGEN) according to the manufacturers’ instructions. The RNA was quantified using Ribogreen RNA quantification kit (Molecular Probes). For each sample, 250 ng of RNA was reverse transcribed in a total volume of 30 μl using random hexamer primers (Fermentas Life Sciences). Real-time RT-PCR was performed in triplicate using an Applied Biosystems 7900 HT system and carboxy fluorescein–labeled (FAM-labeled) Assay-on-Demand reagent sets (Applera Corp.) for the following: FoxP3, Hs00203958_m1; IL-10, Hs00174086_m1; and TGF-β, Hs99999918_m1. Real-time RT-PCR reactions were multiplexed using VIC-labeled 18s primers and probes (Hs99999901_s1) as endogenous control and analyzed using SDS software version 2.1 (Applied Biosystems) according to the 2–(ΔΔCt) method.
Results are presented as mean ± SEM. Differences between groups were assessed using the 2-tailed paired Student’s t test. Differences were considered significant at the 95% confidence level.
E. Xystrakis and S. Kusumakar were funded by Asthma-UK (00/023 and 03/065). T. Adikibi was funded by a UK Medical Research Council Realising Our Potential Award. T.H. Lee and P. Lavender were funded by a UK Medical Research Council Programme Grant.
Sandra Boswell and Emma Peek contributed equally to this work.
Emmanuel Xystrakis and Siddharth Kusumakar are joint first authors.
Nonstandard abbreviations used: FEV1, forced expired volume in 1 second; GR, glucocorticoid receptor; SR, glucocorticoid resistant; SS, glucocorticoid sensitive.
Conflict of interest: The authors have declared that no conflict of interest exists.