Search tips
Search criteria 


Logo of jrsocmedLink to Publisher's site
J R Soc Med. 1987 August; 80(8): 495–498.
PMCID: PMC1290956

Intracellular metabolites in rat muscle following trauma: a 31P and 1H nuclear magnetic resonance study.


Hind limb skeletal muscle was studied in vivo in a rat trauma model using nuclear magnetic resonance (NMR) spectroscopy. The model used was a 25% body surface area, full-thickness burn administered under anaesthesia. Two groups of six rats were studied. Weight loss was observed in the experimental group whilst the control group continued to gain weight. Concentration ratios involving intramyocellular phosphocreatine (PCr), creatine (Cr), adenosine triphosphate (ATP), inorganic phosphate (Pi), anserine (Ans) and taurine (Tau) were measured. No change in the ratios of PCr/Pi, PCr/ATP, Ans/PCr + Cr and Tau/PCr + Cr were seen between the two groups. Intracellular pH was the same in the two groups. NMR spectroscopy in vivo gives values of Pi and PCr that differ from those obtained by conventional techniques. NMR values are probably more accurate as no degradation occurs during measurement, the measurements being repeatable and noninvasive.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (847K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Lovén L, Larsson J, Lennquist S, Liljedahl SO. Hypophosphataemia and muscle phosphate metabolism in severely injured patients. Acta Chir Scand. 1983;149(8):743–749. [PubMed]
  • Lovén L, Andersson E, Larsson J, Lennquist S. Muscular high-energy phosphates and red-cell 2,3-DPG in post-traumatic hypophosphataemia. An experimental study in pigs. Acta Chir Scand. 1983;149(8):735–741. [PubMed]
  • Stinnett JD, Alexander JW, Watanabe C, MacMillan BG, Fischer JE, Morris MJ, Trocki O, Miskell P, Edwards L, James H. Plasma and skeletal muscle amino acids following severe burn injury in patients and experimental animals. Ann Surg. 1982 Jan;195(1):75–89. [PubMed]
  • Bergström J, Boström H, Fürst P, Hultman E, Vinnars E. Preliminary studies of energy-rich phosphagens in muscle from severely ill patients. Crit Care Med. 1976 Jul-Aug;4(4):197–204. [PubMed]
  • Edwards RH. Percutaneous needle-biopsy of skeletal muscle in diagnosis and research. Lancet. 1971 Sep 11;2(7724):593–595. [PubMed]
  • Bergstrom J. Percutaneous needle biopsy of skeletal muscle in physiological and clinical research. Scand J Clin Lab Invest. 1975 Nov;35(7):609–616. [PubMed]
  • Newman JJ, Strome DR, Goodwin CW, Mason AD, Jr, Pruitt BA., Jr Altered muscle metabolism in rats after thermal injury. Metabolism. 1982 Dec;31(12):1229–1233. [PubMed]
  • Taylor DJ, Bore PJ, Styles P, Gadian DG, Radda GK. Bioenergetics of intact human muscle. A 31P nuclear magnetic resonance study. Mol Biol Med. 1983 Jul;1(1):77–94. [PubMed]
  • Moon RB, Richards JH. Determination of intracellular pH by 31P magnetic resonance. J Biol Chem. 1973 Oct 25;248(20):7276–7278. [PubMed]
  • Ackerman JJ, Grove TH, Wong GG, Gadian DG, Radda GK. Mapping of metabolites in whole animals by 31P NMR using surface coils. Nature. 1980 Jan 10;283(5743):167–170. [PubMed]
  • Nordström H, Lennquist S, Lindell B, Sjöberg HE. Hypophosphataemia in severe burns. Acta Chir Scand. 1977;143(7-8):395–399. [PubMed]
  • Karner J, Roth E, Funovics J, Hanusch J, Walzer L, Adamiker D, Berger A, Meissl G. Effects of burns on amino acid levels in rat plasma, liver and muscle. Burns Incl Therm Inj. 1984 Dec;11(2):130–137. [PubMed]
  • Thurston JH, Hauhart RE, Naccarato EF. Taurine: possible role in osmotic regulation of mammalian heart. Science. 1981 Dec 18;214(4527):1373–1374. [PubMed]
  • Askanazi J, Carpentier YA, Michelsen CB, Elwyn DH, Furst P, Kantrowitz LR, Gump FE, Kinney JM. Muscle and plasma amino acids following injury. Influence of intercurrent infection. Ann Surg. 1980 Jul;192(1):78–85. [PubMed]
  • Snelling CF, Woolf LI, Groves AC, Moore JP, Duff JH. Amino acid metabolism in patients with severe burns. Surgery. 1982 Apr;91(4):474–481. [PubMed]
  • Radda GK, Bore PJ, Rajagopalan B. Clinical aspects on 31P NMR spectroscopy. Br Med Bull. 1984 Apr;40(2):155–159. [PubMed]

Articles from Journal of the Royal Society of Medicine are provided here courtesy of Royal Society of Medicine Press