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J R Soc Med. 1987 August; 80(8): 482–484.
PMCID: PMC1290951

Effect of two disinfectant treatments on laboratory analyses.


Inactivation of human immunodeficiency virus (HIV), the retrovirus associated with the acquired immune deficiency syndrome (AIDS), can be achieved both by heating at 56 degrees C and by chemical disinfection with beta-propiolactone (BPL). Such treatment of specimens from patients with suspected AIDS or antibodies to HIV could reduce any occupational risk to laboratory staff. This study demonstrates the effects of these treatments on laboratory analyses. Heat treatment of plasma caused clinically insignificant alteration in the results of electrolyte, urea, creatinine, albumin and glucose concentration, but significant alteration to total protein estimation and the activities of many enzymes. Haemolysis and cell agglutination occurred in many specimens of whole blood, making them unsuitable for haematological analyses. BPL caused less reduction of enzyme activities, and assay of many analytes, including electrolytes, plasma proteins, haemoglobin, white cell and platelet counts, was not significantly affected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Jones P, Hamilton P. HTLV-III antibodies in haematology staff. Lancet. 1985 Jan 26;1(8422):217–217. [PubMed]
  • Shanson DC, Evans R, Lai L. Incidence and risk of transmission of HTLV III infections to staff at a London hospital, 1982-85. J Hosp Infect. 1985 Dec;6 (Suppl 100):15–22. [PubMed]
  • Geddes AM. Risk of AIDS to health care workers. Br Med J (Clin Res Ed) 1986 Mar 15;292(6522):711–712. [PMC free article] [PubMed]
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  • Lloyd G, Bowen ET, Slade JH. Physical and chemical methods of inactivating Lassa virus. Lancet. 1982 May 8;1(8280):1046–1048. [PubMed]
  • LOGRIPPO GA. Investigations of the use of beta-propiolactone in virus inactivation. Ann N Y Acad Sci. 1960 Jan 13;83:578–594. [PubMed]

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