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Logo of cjvetresCVMACanadian Journal of Veterinary ResearchSee also Canadian Journal of Comparative MedicineJournal Web siteHow to Submit
 
Can J Vet Res. 1989 April; 53(2): 172–175.
PMCID: PMC1255543

Protein A gold identification of ureaplasmas on the bovine zona pellucida.

Abstract

The object of this study was to develop a prefixation protein A gold labelling technique for Ureaplasma diversum and to apply this to bovine embryos. Sixteen hour cultures of Ureaplasma diversum strain 2312 were incubated with either specific antiserum or nonimmune serum, followed by exposure to protein A gold and negative staining. The ureaplasmas which were incubated with specific antiserum were labelled with gold particles while those ureaplasmas which were incubated with nonimmune serum were not labelled. Twenty-three unhatched, day 7 bovine embryos were then incubated in either embryo culture medium (ECM) alone, ECM with sterile ureaplasma broth added or ECM with 1.7 X 10(6) colony forming units of Ureaplasma diversum strain 2312 per embryo. After 16 hours, the embryos were washed twice and incubated with either specific antiserum or nonimmune serum. The embryos were then incubated with medium containing protein A gold and examined by electron microscopy. No ureaplasmas were identified on the zona pellucida of the control embryos. Ureaplasmas were identified on the outer surface of the zona pellucida of 13 of the 17 embryos which had been exposed to the organism. Of these, the embryos which were incubated with specific antiserum had labelled ureaplasmas while the embryos which were incubated with nonimmune serum had unlabelled ureaplasmas on the zona pellucida. It was concluded that the protein A gold method was a suitable technique for the identification of ureaplasmas in EM preparations. The presence of ureaplasmas on the outer surface of the bovine zona pellucida following in vitro exposure to the organism was confirmed.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  • Singh EL, Thomas FC, Papp-Vid G, Eaglesome MD, Hare WC. Embryo transfer as a means of controlling the transmission of viral infections. II. The in vitro exposure of preimplantation bovine embryos to infectious bovine rhinotracheitis virus. Theriogenology. 1982 Aug;18(2):133–140. [PubMed]
  • Singh EL, Eaglesome MD, Thomas FC, Papp-Vid G, Hare WC. Embryo transfer as a means of controlling the transmission of viral infections. I. The in vitro exposure of preimplantation bovine embryos to akabane, bluetongue and bovine viral diarrhea viruses. Theriogenology. 1982 Apr;17(4):437–444. [PubMed]
  • Lauerman LH, Stringfellow DA, Sparling PH, Kaub LM. In vitro exposure of preimplantation bovine embryos to vesicular stomatitis virus. J Clin Microbiol. 1986 Sep;24(3):380–383. [PMC free article] [PubMed]
  • Bowen RA, Howard TH, Elsden RP, Seidel GE., Jr Embryo transfer from cattle infected with bluetongue virus. Am J Vet Res. 1983 Sep;44(9):1625–1628. [PubMed]
  • Singh EL, McVicar JW, Hare WC, Mebus CA. Embryo transfer as a means of controlling the transmission of viral infections. VII. The in vitro exposure of bovine and porcine embryos to foot-and-mouth disease virus. Theriogenology. 1986 Nov;26(5):587–593. [PubMed]
  • Hare WC, Mitchell D, Singh EL, Bouillant AM, Eaglesome MD, Ruckerbauer GM, Bielanski A, Randall GC. Embryo transfer in relation to bovine leukemia virus control and eradication. Can Vet J. 1985 Aug;26(8):231–234. [PMC free article] [PubMed]
  • Britton AP, Ruhnke HL, Miller RB, Johnson WH, Leslie KE, Rosendal S. In vitro exposure of bovine morulae to Ureaplasma diversum. Can J Vet Res. 1987 Apr;51(2):198–203. [PMC free article] [PubMed]
  • Ruhnke HL, Doig PA, MacKay AL, Gagnon A, Kierstead M. Isolation of Ureaplasma from bovine granular vulvitis. Can J Comp Med. 1978 Apr;42(2):151–155. [PMC free article] [PubMed]
  • Doig PA, Ruhnke HL, MacKay AL, Palmer NC. Bovine granular vulvitis associated with ureaplasma infection. Can Vet J. 1979 Apr;20(4):89–94. [PMC free article] [PubMed]
  • Doig PA, Ruhnke HL, Palmer NC. Experimental bovine genital ureaplasmosis. I. Granular vulvitis following vulvar inoculation. Can J Comp Med. 1980 Jul;44(3):252–258. [PMC free article] [PubMed]
  • Doig PA, Ruhnke HL, Palmer NC. Experimental bovine genital ureaplasmosis. II. Granular vulvitis, endometritis and salpingitis following uterine inoculation. Can J Comp Med. 1980 Jul;44(3):259–266. [PMC free article] [PubMed]
  • Gale SP. The effects of two Ureaplasma diversum strains on early pregnancy in heifers. Can J Vet Res. 1987 Oct;51(4):536–538. [PMC free article] [PubMed]
  • Kreplin CM, Ruhnke HL, Miller RB, Doig PA. The effect of intrauterine inoculation with Ureaplasma diversum on bovine fertility. Can J Vet Res. 1987 Oct;51(4):440–443. [PMC free article] [PubMed]
  • Neighbour PA. Studies on the susceptibility of the mouse preimplantation embryo to infection with cytomegalovirus. J Reprod Fertil. 1978 Sep;54(1):15–20. [PubMed]
  • Shea BF. Evaluating the bovine embryo. Theriogenology. 1981 Jan;15(1):31–42. [PubMed]
  • Whitescarver J, Furness G. T-mycoplasmas: a study of the morphology, ultrastructure and mode of division of some human strains. J Med Microbiol. 1975 May;8(2):349–355. [PubMed]
  • Black FT, Birch-Andersen A, Freundt EA. Morphology and ultrastructure of human T-mycoplasmas. J Bacteriol. 1972 Jul;111(1):254–259. [PMC free article] [PubMed]

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