Search tips
Search criteria 


Logo of cjvetresCVMACanadian Journal of Veterinary ResearchSee also Canadian Journal of Comparative MedicineJournal Web siteHow to Submit
Can J Vet Res. 1987 October; 51(4): 440–443.
PMCID: PMC1255361

The effect of intrauterine inoculation with Ureaplasma diversum on bovine fertility.


To determine the influence of Ureaplasma diversum on bovine fertility 11 uninfected virgin heifers with normal ovarian cyclic activity were randomly allocated to test or control groups. At a synchronized estrus, five test heifers were given an intrauterine broth inoculum containing 1.09 x 10(8) to 1.4 x 10(9) colony forming units of U. diversum and six control animals were infused with sterile ureaplasma broth medium. All animals were artificially inseminated within one hour of infusion. Pregnancy was diagnosed in one of five test heifers and all of six controls by serum progesterone concentrations measured to 25 days postinsemination. The difference in pregnancy rates between the two groups was statistically significant (p = 0.0152). It was concluded that under the conditions of this experiment U. diversum is capable of causing infertility in cattle.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (596K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Taylor-Robinson D, Haig DA, Williams MH. Bovine T-strain mycoplasma. Ann N Y Acad Sci. 1967 Jul 28;143(1):517–518. [PubMed]
  • Langford EV. Mycoplasma species recovered from the reproductive tracts of western Canadian cows. Can J Comp Med. 1975 Apr;39(2):133–138. [PMC free article] [PubMed]
  • Onoviran O, Truscott RB, Fish NA, Barker CA, Ruhnke HL. The recovery of Mycoplasmas from the genital tracts of bulls in artificial breeding units in Ontarïo. Can J Comp Med. 1975 Oct;39(4):474–475. [PMC free article] [PubMed]
  • Anderson NG. Mycoplasma genital tract infections in cattle. Can Vet J. 1974 Mar;15(3):95–95. [PMC free article] [PubMed]
  • Ruhnke HL, Doig PA, MacKay AL, Gagnon A, Kierstead M. Isolation of Ureaplasma from bovine granular vulvitis. Can J Comp Med. 1978 Apr;42(2):151–155. [PMC free article] [PubMed]
  • Doig PA, Ruhnke HL, MacKay AL, Palmer NC. Bovine granular vulvitis associated with ureaplasma infection. Can Vet J. 1979 Apr;20(4):89–94. [PMC free article] [PubMed]
  • Panangala VS, Fish NA, Barnum DA. Microflora of the cervico-vaginal mucus of repeat breeder cows. Can Vet J. 1978 Apr;19(4):83–89. [PMC free article] [PubMed]
  • Appleby RC, Gourlay RN. Examination of uterine biopsies from infertile cows for the presence of viruses and mycoplasmas. Res Vet Sci. 1980 Mar;28(2):223–227. [PubMed]
  • Doig PA, Ruhnke HL, Palmer NC. Experimental bovine genital ureaplasmosis. II. Granular vulvitis, endometritis and salpingitis following uterine inoculation. Can J Comp Med. 1980 Jul;44(3):259–266. [PMC free article] [PubMed]
  • Miller RB, Ruhnke HL, Doig PA, Poitras BJ, Palmer NC. The effects of Ureplasma diversum inoculated into the amniotic cavity in cows. Theriogenology. 1983 Sep;20(3):367–374. [PubMed]
  • Ball HJ, McCaughey WJ. Distribution of mycoplasmas within the urogenital tract of the cow. Vet Rec. 1979 May 26;104(21):482–483. [PubMed]
  • Johnson CT. Time to onset of oestrus after the injection of heifers with cloprostenol. Vet Rec. 1978 Sep 2;103(10):204–206. [PubMed]
  • Doig PA, Ruhnke HL, Palmer NC. Experimental bovine genital ureaplasmosis. I. Granular vulvitis following vulvar inoculation. Can J Comp Med. 1980 Jul;44(3):252–258. [PMC free article] [PubMed]
  • Ruhnke HL, Palmer NC, Doig PA, Miller RB. Bovine abortion and neonatal death associated with Ureaplasma diversum. Theriogenology. 1984 Feb;21(2):295–301. [PubMed]
  • Rosendal S, Black FT. Direct and indirect immunofluorescence of unfixed and fixed Mycoplasma colonies. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(4):615–622. [PubMed]
  • Stephens LR, Humphrey JD, Little PB, Barnum DA. Morphological, biochemical, antigenic, and cytochemical relationships among Haemophilus somnus, Haemophilus agni, Haemophilus haemoglobinophilus, Histophilus ovis, and Actinobacillus seminis. J Clin Microbiol. 1983 May;17(5):728–737. [PMC free article] [PubMed]
  • Thornber PM. Ureaplasma association with bovine infertility in south-west Scotland. Vet Rec. 1982 Dec 18;111(25-26):591–591. [PubMed]
  • Gowan EW, Etches RJ, Bryden C, King GJ. Factors affecting accuracy of pregnancy diagnosis in cattle. J Dairy Sci. 1982 Jul;65(7):1294–1302. [PubMed]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association