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Environ Health Perspect. 2004 March; 112(3): 353–358.
PMCID: PMC1241866
Research Article

Endocrine-disrupting effects of cattle feedlot effluent on an aquatic sentinel species, the fathead minnow.

Abstract

Over the last decade, research has examined the endocrine-disrupting action of various environmental pollutants, including hormones, pharmaceuticals, and surfactants, in sewage treatment plant effluent. Responding to the growth of concentrated animal feeding operations (CAFOs) and the pollutants present in their wastewater (e.g., nutrients, pharmaceuticals, and hormones), the U.S. Environmental Protection Agency developed a new rule that tightens the regulation of CAFOs. In this study, we collected wild fathead minnows (Pimephales promelas) exposed to feedlot effluent (FLE) and observed significant alterations in their reproductive biology. Male fish were demasculinized (having lower testicular testosterone synthesis, altered head morphometrics, and smaller testis size). Defeminization of females, as evidenced by a decreased estrogen:androgen ratio of in vitro steroid hormone synthesis, was also documented. We did not observe characteristics in either male or female fish indicative of exposure to environmental estrogens. Using cells transfected with the human androgen receptor, we detected potent androgenic responses from the FLE. Taken together, our morphologic, endocrinologic, and in vitro gene activation assay data suggest two hypotheses: a) there are potent androgenic substance(s) in the FLE, and/or b) there is a complex mixture of androgenic and estrogenic substances that alter the hypothalamic-pituitary-gonadal axis, inhibiting the release of gonadotropin-releasing hormone or gonadotropins. This is the first study demonstrating that the endocrine and reproductive systems of wild fish can be adversely affected by FLE. Future studies are needed to further investigate the effects of agricultural runoff and to identify the biologically active agents, whether natural or pharmaceutical in origin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ankley GT, Jensen KM, Kahl MD, Korte JJ, Makynen EA. Description and evaluation of a short-term reproduction test with the fathead minnow (Pimephales promelas). Environ Toxicol Chem. 2001 Jun;20(6):1276–1290. [PubMed]
  • Ankley Gerald T, Jensen Kathleen M, Makynen Elizabeth A, Kahl Michael D, Korte Joseph J, Hornung Michael W, Henry Tala R, Denny Jeffrey S, Leino Richard L, Wilson Vickie S, et al. Effects of the androgenic growth promoter 17-beta-trenbolone on fecundity and reproductive endocrinology of the fathead minnow. Environ Toxicol Chem. 2003 Jun;22(6):1350–1360. [PubMed]
  • Arukwe A, Grotmol T, Haugen TB, Knudsen FR, Goksøyr A. Fish model for assessing the in vivo estrogenic potency of the mycotoxin zearalenone and its metabolites. Sci Total Environ. 1999 Sep 15;236(1-3):153–161. [PubMed]
  • Balter M. Scientific cross-claims fly in continuing beef war. Science. 1999 May 28;284(5419):1453–1455. [PubMed]
  • Daughton CG, Ternes TA. Pharmaceuticals and personal care products in the environment: agents of subtle change? Environ Health Perspect. 1999 Dec;107 (Suppl 6):907–938. [PMC free article] [PubMed]
  • Folmar LC, Denslow ND, Kroll K, Orlando EF, Enblom J, Marcino J, Metcalfe C, Guillette LJ., Jr Altered serum sex steroids and vitellogenin induction in walleye (Stizostedion vitreum) collected near a metropolitan sewage treatment plant. Arch Environ Contam Toxicol. 2001 Apr;40(3):392–398. [PubMed]
  • Folmar LC, Denslow ND, Rao V, Chow M, Crain DA, Enblom J, Marcino J, Guillette LJ., Jr Vitellogenin induction and reduced serum testosterone concentrations in feral male carp (Cyprinus carpio) captured near a major metropolitan sewage treatment plant. Environ Health Perspect. 1996 Oct;104(10):1096–1101. [PMC free article] [PubMed]
  • Guillette LJ, Jr, Gross TS, Gross DA, Rooney AA, Percival HF. Gonadal steroidogenesis in vitro from juvenile alligators obtained from contaminated or control lakes. Environ Health Perspect. 1995 May;103 (Suppl 4):31–36. [PMC free article] [PubMed]
  • Irwin LK, Gray S, Oberdörster E. Vitellogenin induction in painted turtle, Chrysemys picta, as a biomarker of exposure to environmental levels of estradiol. Aquat Toxicol. 2001 Nov 1;55(1-2):49–60. [PubMed]
  • Kolpin Dana W, Furlong Edward T, Meyer Michael T, Thurman E Michael, Zaugg Steven D, Barber Larry B, Buxton Herbert T. Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000: a national reconnaissance. Environ Sci Technol. 2002 Mar 15;36(6):1202–1211. [PubMed]
  • Le Guevel R, Pakdel F. Assessment of oestrogenic potency of chemicals used as growth promoter by in-vitro methods. Hum Reprod. 2001 May;16(5):1030–1036. [PubMed]
  • McLachlan JA. Environmental signaling: what embryos and evolution teach us about endocrine disrupting chemicals. Endocr Rev. 2001 Jun;22(3):319–341. [PubMed]
  • Meyer HH. Biochemistry and physiology of anabolic hormones used for improvement of meat production. APMIS. 2001 Jan;109(1):1–8. [PubMed]
  • Orlando EF, Denslow ND, Folmar LC, Guillette LJ., Jr A comparison of the reproductive physiology of largemouth bass, Micropterus salmoides, collected from the Escambia and Blackwater Rivers in Florida. Environ Health Perspect. 1999 Mar;107(3):199–204. [PMC free article] [PubMed]
  • Parks LG, Lambright CS, Orlando EF, Guillette LJ, Jr, Ankley GT, Gray LE., Jr Masculinization of female mosquitofish in Kraft mill effluent-contaminated Fenholloway River water is associated with androgen receptor agonist activity. Toxicol Sci. 2001 Aug;62(2):257–267. [PubMed]
  • Schiffer B, Daxenberger A, Meyer K, Meyer HH. The fate of trenbolone acetate and melengestrol acetate after application as growth promoters in cattle: environmental studies. Environ Health Perspect. 2001 Nov;109(11):1145–1151. [PMC free article] [PubMed]
  • Soto Ana M, Calabro Janine M, Prechtl Nancy V, Yau Alice Y, Orlando Edward F, Daxenberger Andreas, Kolok Alan S, Guillette Louis J, Jr, le Bizec Bruno, Lange Iris G, et al. Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in Eastern Nebraska, USA. Environ Health Perspect. 2004 Mar;112(3):346–352. [PMC free article] [PubMed]
  • Stumpf M, Ternes TA, Wilken RD, Rodrigues SV, Baumann W. Polar drug residues in sewage and natural waters in the state of Rio de Janeiro, Brazil. Sci Total Environ. 1999 Jan 12;225(1-2):135–141. [PubMed]
  • Tyler CR, Jobling S, Sumpter JP. Endocrine disruption in wildlife: a critical review of the evidence. Crit Rev Toxicol. 1998 Jul;28(4):319–361. [PubMed]
  • Wilson Vickie S, Lambright Christy, Ostby Joe, Gray LE., Jr In vitro and in vivo effects of 17beta-trenbolone: a feedlot effluent contaminant. Toxicol Sci. 2002 Dec;70(2):202–211. [PubMed]

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