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Environ Health Perspect. 2002 August; 110(8): 829–833.
PMCID: PMC1240957
Research Article

Temporal association of children's pesticide exposure and agricultural spraying: report of a longitudinal biological monitoring study.


We measured organophosphorus (OP) pesticide exposures of young children living in an agricultural community over an entire year and evaluated the impact of agricultural spraying on exposure. We also examined the roles of age, sex, parental occupation, and residential proximity to fields. We recruited 44 children (2-5 years old) through a Women, Infants, and Children clinic. We collected urine samples on a biweekly basis over a 21-month period. Each child provided at least 16 urine samples, and most provided 26. We analyzed samples for the dialkylphosphate (DAP) metabolites common to the OP pesticides. DAP concentrations were elevated in months when OP pesticides were sprayed in the region's orchards. The geometric means of dimethyl and diethyl DAPs during spray months were higher than those during nonspray months (p = 0.009 for dimethyl; p = 0.018 for diethyl). Dimethyl DAP geometric means were 0.1 and 0.07 micro mol/L for spray months and nonspray months, respectively (57% difference); diethyl DAP geometric means were 0.49 and 0.35, respectively (40% difference). We also observed differences for sex of the child, with male levels higher than female levels (p = 0.005 for dimethyl; p = 0.046 for diethyl). We observed no differences due to age, parental occupation, or residential proximity to fields. This study reports for the first time the temporal pattern of pesticide exposures over the course of a full year and indicates that pesticide spraying in an agricultural region can increase children's exposure in the absence of parental work contact with pesticides or residential proximity to pesticide-treated farmland.

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Selected References

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  • Simcox NJ, Fenske RA, Wolz SA, Lee IC, Kalman DA. Pesticides in household dust and soil: exposure pathways for children of agricultural families. Environ Health Perspect. 1995 Dec;103(12):1126–1134. [PMC free article] [PubMed]
  • Lu C, Fenske RA, Simcox NJ, Kalman D. Pesticide exposure of children in an agricultural community: evidence of household proximity to farmland and take home exposure pathways. Environ Res. 2000 Nov;84(3):290–302. [PubMed]
  • Faustman EM, Silbernagel SM, Fenske RA, Burbacher TM, Ponce RA. Mechanisms underlying Children's susceptibility to environmental toxicants. Environ Health Perspect. 2000 Mar;108 (Suppl 1):13–21. [PMC free article] [PubMed]
  • Eskenazi B, Bradman A, Castorina R. Exposures of children to organophosphate pesticides and their potential adverse health effects. Environ Health Perspect. 1999 Jun;107 (Suppl 3):409–419. [PMC free article] [PubMed]
  • Loewenherz C, Fenske RA, Simcox NJ, Bellamy G, Kalman D. Biological monitoring of organophosphorus pesticide exposure among children of agricultural workers in central Washington State. Environ Health Perspect. 1997 Dec;105(12):1344–1353. [PMC free article] [PubMed]
  • Azaroff LS. Biomarkers of exposure to organophosphorous insecticides among farmers' families in rural El Salvador: factors associated with exposure. Environ Res. 1999 Feb;80(2 Pt 1):138–147. [PubMed]
  • Aprea C, Strambi M, Novelli MT, Lunghini L, Bozzi N. Biologic monitoring of exposure to organophosphorus pesticides in 195 Italian children. Environ Health Perspect. 2000 Jun;108(6):521–525. [PMC free article] [PubMed]
  • Lu C, Knutson DE, Fisker-Andersen J, Fenske RA. Biological monitoring survey of organophosphorus pesticide exposure among pre-school children in the Seattle metropolitan area. Environ Health Perspect. 2001 Mar;109(3):299–303. [PMC free article] [PubMed]
  • MacIntosh DL, Needham LL, Hammerstrom KA, Ryan PB. A longitudinal investigation of selected pesticide metabolites in urine. J Expo Anal Environ Epidemiol. 1999 Sep-Oct;9(5):494–501. [PubMed]
  • Moate TF, Lu C, Fenske RA, Hahne RM, Kalman DA. Improved cleanup and determination of dialkyl phosphates in the urine of children exposed to organophosphorus insecticides. J Anal Toxicol. 1999 Jul-Aug;23(4):230–236. [PubMed]
  • Hubal EA, Sheldon LS, Zufall MJ, Burke JM, Thomas KW. The challenge of assessing children's residential exposure to pesticides. J Expo Anal Environ Epidemiol. 2000 Nov-Dec;10(6 Pt 2):638–649. [PubMed]
  • Zartarian VG, Ozkaynak H, Burke JM, Zufall MJ, Rigas ML, Furtaw EJ., Jr A modeling framework for estimating children's residential exposure and dose to chlorpyrifos via dermal residue contact and nondietary ingestion. Environ Health Perspect. 2000 Jun;108(6):505–514. [PMC free article] [PubMed]
  • Shurdut BA, Barraj L, Francis M. Aggregate exposures under the Food Quality Protection Act: An approach using chlorpyrifos. Regul Toxicol Pharmacol. 1998 Oct;28(2):165–177. [PubMed]
  • Melnyk LJ, Berry MR, Sheldon LS. Dietary exposure from pesticide application on farms in the Agricultural Health Pilot Study. J Expo Anal Environ Epidemiol. 1997 Jan-Mar;7(1):61–80. [PubMed]

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