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J Exp Med. 2005 February 7; 201(3): 341–348.
PMCID: PMC1237122
NIHMSID: NIHMS2077

Direct in vivo VH to JH rearrangement violating the 12/23 rule

Abstract

V(D)J recombination at the immunoglobulin heavy chain (IgH) locus follows the 12/23 rule to ensure the correct assembly of the variable region gene segments. Here, we report characterization of an in vivo model that allowed us to study recombination violating the 12/23 rule, namely a mouse strain lacking canonical D elements in its IgH locus. We demonstrate that VH to JH joining can support the generation of all B cell subsets. However, the process is inefficient in that B cells and antibodies derived from the DH-less allele are not detectable if the latter is combined with a wild-type IgH allele. There is no preferential usage of any particular VH gene family or JH element in VHJH junctions, indicating that 23/23-guided recombination is possible, but is a low frequency event at the IgH locus in vivo.

B and T cell antigen receptor genes are assembled from variable (V), diversity (D), and joining (J) segments in a process termed V(D)J recombination. V(D)J recombination requires the lymphoid-specific proteins RAG1 and RAG2 as well as ubiquitous DNA repair factors. The coding sequences of V, D, and J gene segments are flanked by recombination signal sequences (RSSs). Each RSSs consists of a highly conserved heptamer and a nanomer separated by a spacer of either 12 or 23 base pairs (1).

V(D)J recombination is initiated by the introduction of DNA double-strand breaks at one 12 RSS and one 23 RSS by RAG1/2. The coding segments are fused to produce a coding joint and the RSS are assembled to create a signal joint. In the murine IgH locus, the V and J elements are flanked by RSSs with a 23-bp spacer and the D elements are flanked on both sides by RSSs with a 12-bp spacer, thus insuring that direct VH to JH joining does not occur. As a rule, the segments to be recombined are flanked by RSSs of dissimilar length. This phenomenon, referred to as the 12/23 rule, ensures correct assembly of VDJ joints (2).

In vitro assays by Gellert et al. (3) as well as other groups (4, 5) have demonstrated a strong preference for dissimilar partners regardless of whether the RAG/DNA synapse formation begins at a 12 or at 23 RSS. However, although incorporation of a similar RSS partner was undetectable when RAG1/2 was assembled on the 12 RSS, incorporation of a 23-RSS compared with a 12-RSS partner was only sixfold reduced when synapse formation was initiated on the 23 RSS (3).

We turned our attention to a mouse cloned from the nucleus of a lymph node B cell (6) in search of an in vivo model for VH replacement, a process in which a new VH element “invades” and replaces the VH element used in a rearranged V(D)J joint. The B cell nucleus that gave rise to this mouse contained two rearranged IgH alleles, one of which was in-frame. The other IgH locus was nonproductively rearranged, carrying an elusive rearrangement that could not be characterized using standard PCR for VDJ/DJ joint amplification. In the course of identifying the nature of this rearrangement, we were surprised to find an IgH locus lacking canonical DH elements. Although this allele is not suited to analyze VH replacement, it provides a unique opportunity to study possible exceptions to the 12/23 rule of VDJ recombination at the IgH locus in vivo. Indeed, DH-less mice generate small numbers of B cells whose IgH chains likely result from direct VH to JH joining.

Results AND Discussion

Characterization of the nonproductive IgH gene rearrangement in the LN1 mouse

We set out to identify the nature of the nonproductive rearrangement in the mouse generated from a LN B cell (LN1) by means of a genomic PCR walk (see Materials and Methods) because standard PCR approaches using cocktails of VH and DH gene-specific primers and primers 3′ of the JH elements did not allow us to amplify this rearrangement (6). We identified a rearrangement that used the JH1 element and a sequence immediately upstream of DFL16.1 (Fig. 1). Because DFL16.1 is the most 5′ canonical DH element in the mouse, this rearrangement deletes all the DH elements on the corresponding IgH allele. In addition, the JH1 element is no longer available for further rearrangements because its RSS is deleted. However, the other three JH elements are retained, roughly 98 kb downstream of the most 3′ VH segment. The newly identified allele structure predicts the pattern seen in the Southern analyses describing the LN1 mouse in the original publication (6). We termed this IgH allele ΔD and mice homozygous for ΔD DH-less.

Figure 1.
LN1 nonproductive allele structure. Configurations of wild type and LN1 nonproductive (ΔD) IgH loci are depicted together with proposed intermediates. Nucleotides inserted during the ΔD rearrangement are highlighted in italics and nucleotides ...

The nucleus of the B lymphocyte from which Hochedlinger and colleagues (6) succeeded to clone a mouse had acquired an irregular DH to JH joining event on one of its IgH alleles, leading to the inappropriate joining of a sequence upstream of DFL16.1 to JH1, rather than joining that DH element to JH1 in inverted orientation. Although such an event is probably rare in B cell development, we see no reason why it should have conferred a selective advantage to the nucleus carrying this rearrangement with respect to its ability to allow nuclear cloning. Therefore, we consider the fact that this particular joining event was present in the B cell nucleus from which the mouse was cloned to be a fortunate coincidence, allowing us to study B cell development in mice lacking canonical DH elements in their IgH locus.

DH-less mice are capable of generating B cells

In 5-wk-old DH-less mice, B cells are readily detectable and represent ~5% compared with ~55% of splenocytes in WT animals. Absolute splenic B cell numbers are ~34-fold reduced at this age (Fig. 2 A). In 6-mo-old animals, we saw a significant accumulation of B cells to ~22% of total splenocytes in mutants versus 56% in WT, with absolute numbers of B cells only approximately sevenfold reduced compared with WT (Fig. 2 A). These data are consistent with a low rate of B cell production and mature B cell accumulation with age, and correspond to what is observed in other mouse mutants with impaired B cell production (7). Analyzing mice at the age of 2–3 wk, we observed a ~40-fold reduction in B cell numbers (unpublished data).

Figure 2.
ΔD/ΔD mice generate all peripheral B cell subsets with a low rate of B cell production. Representative flow cytometric analyses of lymphocytes in spleen (A), bone marrow (B), and peritoneal cavity (C) of ΔD/ΔD and WT mice. ...

Marginal zone (MZ) B cells and B1 cell fractions are enlarged in DH-less mice

Based on the CD23/CD21 staining pattern, CD19+CD21int CD23hi follicular (B2) cells were more strongly reduced in the spleens of the mutant animals than MZ B cells (Fig. 2 A). The number of CD19+CD21hiCD23lo MZ B cells increased more than sevenfold from weeks 5 to 25, whereas the increase was less than threefold in control mice. A similar accumulation of MZ B cells has been observed earlier in mice with low rates of B cell generation such as mice lacking the λ5 gene product or both λ5 and κ light chains (unpublished data) and other mouse mutants (8, 9). The B1a (CD5+CD19hi) and B1b (CD5CD19hi) cell populations were not reduced as dramatically as B2 cells in DH-less compared with WT mice, with virtually no reduction in the peritoneal cavity (Fig. 2 C) and an approximately fivefold reduction in the spleen of 5-wk-old mice. B1 cells have been ascribed a self-renewing capacity (10) and this may also pertain to the MZ subset (8).

Most B cell progenitors are blocked at the pro– to pre–B cell transition in DH-less mice

Consistent with the low rate of B cell generation, we detected a block at the pro– to pre–B cell transition with accumulation of IgMB220+CD43+ pro–B cells (Fig. 2 B) in the bone marrow. Only those developing B lymphocytes that receive a signal from a pre–B cell receptor are selected into the pre–B cell compartment (for review see reference 9). Thus, the observed blockade at this developmental stage likely reflects the low probability of generating a gene encoding a functional heavy chain by direct VH to JH joining. There is an approximately eightfold decrease of IgMB220+ CD43 pre–B cells compared with controls, and IgM+B220hi mature recirculating B cells are hardly detectable in the BM of mice homozygous for the ΔD allele (Fig. 2 B).

Homozygous but not heterozygous mutant mice produce cells and antibodies derived from the ΔD allele

In an attempt to compare the efficiencies of classical VDJ recombination and the aberrant joining that occurs at the ΔD allele in a competitive situation, we generated heterozygous mutant mice, with the WT IgH allele of the a allotype from the BALB/c strain. The DH-less allele is derived from the C57BL/6 mice and, thus, is of the b allotype (6). B cells of the IgMb allotype were virtually undetectable in these animals and, in the serum, IgM antibodies of the b allotype were also essentially absent (Fig. 3). In mice homozygous for the ΔD allele, the levels of IgM were similar to those of controls (Fig. 3), whereas the levels of IgG1 were fivefold lower and those of IgA two times higher than in ΔD/+ mice (not depicted).

Figure 3.
The ΔD allele cannot compete with the WT allele in either B cell generation or antibody production. FACS analysis of CD19-gated splenocytes from 5-mo-old ΔD/ΔD, (BALB/c × ΔD)F1, (BALB/c × C57BL/6)F1, C57BL/6, ...

Joints formed at the IgH allele in the DH-less mouse show direct V to J joining

To analyze the gene rearrangements in the IgH locus that allowed for the production of B cells in DH-less mice, we examined the sequences of VH to JH joints in homozygous DH-less mice or ΔD/JHT mice. The JHT allele is not able to encode IgH chains because it lacks JH elements (11). We amplified IgH rearrangements from the B cells of these mice using three different approaches: PCR with single cell DNA as a template, followed by direct sequencing (12) as well as RT-PCR using RNA from FACS-purified B cells and PCR using DNA from magnetic-activated cell sorting (MACS)-purified B cells followed by cloning and sequencing (see Materials and Methods). Fig. 4 depicts sequences obtained by these three approaches, with joints amplified by RT-PCR shown in detail (Fig. 4 A) and the fractions of productive and nonproductive rearrangements for the two DNA-based methods (Fig. 4 B). 66 out of 71 recombination products in the DH-less mice were likely resulting from direct VH to JH joining. However, five joints used a single, previously identified putative DH element, DST4.2 (13), which is still present in the ΔD allele. In the sequence between VH81X and DFL16.1 (as currently available from Ensembl), we found only two pairs of inverted heptamers that are separated by <150 bp: DST4.2, which is flanked by poorly conserved RSSs, and another 10-bp-long sequence flanked by canonical heptamers but lacking recognizable nanomers (GenBank/EMBL/DDBJ accession no. AY841982). We did not detect any remnants of this 10-bp sequence in any of the 71 sequences. We screened all stretches of N/P nucleotides of six bases or more in our sequences against the 97-kb germline sequence between VH81X and DFL16.1 to make sure that they did not reappear in the sequence flanked by sequences resembling heptamers and found no such case. All three JH elements remaining in the ΔD allele and a spectrum of VH gene families were used in our sequence collection (Fig. 4 A). We analyzed the RSSs of the VH genes used in the 66 sequences likely reflecting direct VH to JH joining and found that the distance between canonical heptamers and nanomers of these VH genes was 22–23 nucleotides with no sequence resembling a nanomer in the spacer (unpublished data). We conclude that the process allowing the formation of a productive joint on the ΔD allele in 66 out of the 71 sequences is likely direct recombination between VH and JH elements, both flanked by 23 RSSs.

Figure 4.
Analysis of IgH V region joints derived from B cells in ΔD mice. (A) Alignment of joints amplified from the RNA of 2-wk-old ΔD/ΔD and ΔD/JHT mice. The joints are shown from the codon immediately 5′ of the second ...

In the 66 VHJH joints, we observed an average of ~3 P and N nucleotide insertions per junction (Fig. 4), compatible with a single recombination event (14, 15). We found that the CDR3 regions amplified from the ΔD/ΔD B cells are shorter than the WT CDR3s, and that this reduction in length corresponds to the average number of nucleotides inserted in one round of N and P nucleotide addition and the average length of a DH sequence in WT VHDHJH joints (Fig. 4 C). Looking at VHJH junctions from 2-, 5-, and 10-wk-old mice, no obvious selection over time of cells expressing antibodies with longer CDR3s became apparent. Among the sequences amplified from the DNA of B cells from homozygous mutant mice, we detected only a single, potentially nonproductive joint with a stop codon in the VH gene segment in 39 analyzed sequences from ΔD/ΔD B cells. All other joints were productive. In contrast, out of 71 joints amplified from WT B cells, we found 24 to be nonproductive, either due to out-of-frame joining or stop codons in the joint (Fig. 4 B). From this, we conclude that direct recombination between VH and JH elements is a rare event in vivo, as cells that have acquired a nonproductive VHDHJH joint on one allele and proceeded to rearrange the second allele are apparently missing or very rare in the ΔD/ΔD mice.

Inefficiency of VH to JH joining is likely the main explanation for the ΔD allele deficiency

Mice carrying the ΔD allele at both IgH loci show a severe block of B cell development at the pro–B to pre–B transition, but small numbers of B cells were generated, and these B cells expressed IgH chains that resulted from direct VH to JH joining as demonstrated by sequence analysis of the corresponding gene rearrangements. All subsets of mature peripheral B cells were detectable in DH-less mice. Therefore, DH elements are not essential for the generation of antibody specificities that drive follicular, MZ, or B1 cell differentiation (16).

However, strikingly, the ΔD allele was unable to successfully compete with a WT IgH locus in heterozygous mutant mice in the generation of the antibody repertoire. In these animals, essentially all B cells expressed IgH chains encoded by the WT IgH locus, and there were almost no antibodies with IgH chains encoded by the ΔD allele present in the blood. One reason for the almost complete absence of cells expressing the ΔD allele is clearly the inefficiency of VH to JH joining, as is apparent from the block in B cell development in homozygous mutant mice and the almost exclusive presence of productive VHJH joints in the B cells generated in these animals. The latter finding indicates that the mutant cells do not have sufficient time in development to undergo successive rearrangements at the two homologous IgH loci. Given this situation, it is expected and indeed experimentally found (Fig. 3) that B cells expressing the WT rather than the mutant IgH locus vastly outnumber cells expressing the ΔD allele in heterozygous mutants. B cells expressing the WT allele may have an additional advantage in populating the peripheral immune system in that they express a broader repertoire of antibody specificities. However, even in the bone marrow, immature B cells expressing the ΔD allele are hardly detectable in the heterozygous mutant mice (Fig. 5). Therefore, inefficiency of VH to JH joining is apparently the main cause of the inability of the ΔD allele to compete with a WT IgH allele in vivo.

Figure 5.
Absence of newly generated B cells expressing the ΔD allele in heterozygous mutant mice. Immature and mature B cells in the bone marrow of 10-mo-old (BALB/c × ΔD)F1 mice were compared with those of 5-mo-old (BALB/c × C57BL/6)F1, ...

Materials and Methods

Cloning of the nonproductive IgH gene rearrangement from the LN1 mouse.

Genomic DNA was prepared from LN1 ES cells (6) using the Genomic DNA Kit according to the manufacturer's instructions (QIAGEN). To isolate the unknown sequence fused to the JH region in the nonproductively rearranged IgH allele of the LN1 mouse, a “pan”-PCR genome walking strategy was performed using adaptor AP1 and adaptor specific primers A1 and A2 (GenomeWalker Kit; CLONTECH Laboratories, Inc.) as described in the original publication by the inventors (17). The gene-specific primers JH4E and JH4A have been described previously (12). The resulting amplification product of 1.7 kb in length was cloned and sequenced to reveal a rearrangement that used the JH1 element and a sequence immediately upstream of DFL16.1 (Fig. 1).

Preparative and analytical FACS and MACS.

Fluorescence staining was performed as described previously (18). Antibodies were conjugated to FITC, PE, APC, PerCP, Cy-Chrome, or biotin. Biotinylated antibodies were developed with streptavidin conjugated to Cy-Chrome or PerCP. Stained cells were analyzed on a FACScalibur (Becton Dickinson). Cell sorting was performed using a triple laser flow cytometer (FACSVantage; Becton Dickinson). Single splenic B and T cells were directly deposited into PCR 96-well plates containing 20 μl 1× PCR buffer (2.5 mM MgCl2; GIBCO BRL), immediately frozen on dry ice, and stored at −80°C. Single cells from the E14 embryonic stem cell line (19) were isolated in a similar way as negative controls for the PCR. Beads were used to confirm that one cell only was deposited in a microtiter plate well; this was further confirmed by the fact that we never saw more than two rearrangements per well in sorted WT B cells.

For bulk analysis, 5 × 104 splenic B cells were sorted into TRIzol (Invitrogen)-containing tubes. T cells from the same cell suspension were sorted in a similar manner as negative controls for the analyses of IgH gene rearrangements.

In a separate experiment, CD19 MACS beads and LS columns (Miltenyi Biotec) were used to separate B cells from whole spleens. The purity of the cells was assessed by B220/CD19 staining. The resulting cell populations contained 90–96% B cells. Mice were kept according to Harvard guidelines.

Single cell PCR

To prepare DNA for amplification, 1 μl of an aqueous solution of proteinase K (10 mg/ml; Boehringer) was added to frozen single cell containing tubes and samples were overlaid with paraffin oil and incubated for 30 min at 55°C. Subsequently, proteinase K was inactivated for 10 min at 95°C. PCR amplification was performed in two rounds: the first reaction contained a mix of all VH family specific primers and the JH4E primer (12). Amplification was performed over 30 cycles (1 min at 95°C, 1 min at 60°C, and 2.5 min at 72°C). For the second round of amplification, 1.5-μl aliquots of the product of the first round were transferred into separate reactions (set up in 96-well microtiter plates), each containing 7 pmol of a single 5′ primer in combination with 7 pmol of the nested JH4A primer (13). 30 cycles were performed (1 min at 95°C, 1 min at 63°C, and 1.5 min at 72°C). All PCRs contained dATP, dCTP, dGTP, and dTTP (Amersham Biosciences) at 200 μM each, PCR buffer (Eppendorf), 2.5 mM Mg2+, and 5 U Taq DNA polymerase (Eppendorf) in the first round, and 3 U in the second round. The final volume of each reaction was 50 μl. Each PCR was followed by a 5–10-min incubation at 72°C. 10 μl of the second-round PCR product was analyzed on agarose gels. Before sequencing, 1.5 μl of second-round product was reamplified for 20 cycles (30 s at 95°C, 1 min at 63°C, and 2 min at 72°C) using appropriate 5′ primers and nested 3′ primers. Sequencing was performed at the High-throughput DNA Sequencing Facility of the Dana Farber/Harvard Cancer Center. The primers used for amplification and sequencing of V(D)J rearrangements have been described by Ehlich et al. (12) and Löffert et al. (20).

Bulk RT-PCR

RNA was extracted from sorted cells according to the TRIzol (Invitrogen) manufacturer's protocol starting with 5 × 104 cells. cDNA was prepared using the oligo-dT priming the same day. 2 μl of the cDNA was used for further amplification of the VDJ joints. Two rounds of amplification using a primer specific for the majority of mouse VH genes MsVHE (15) and nested constant region primers CμE and CμA (21) for the first and second rounds, correspondingly. 35 cycles were performed for each round (30 s at 97°C, 30 s at 50°C, and 30 s at 72°C). The expected 350-bp size was purified from the gel and subcloned into the TOPO TA vector (Invitrogen). DNA from individual colonies was prepared and sequenced using standard vector specific primers.

Sequence analysis of Ig rearrangements

Sequences were analyzed using www.dnaplot.de, IMGT.cines.fr, and IgBlast–based programs. The databases used consist of mouse V gene sequences from a GenBank/EMBL/DDBJ nucleotide sequence database, a Kabat database (22), and the V gene sequences compiled by Lefranc (2326).

Acknowledgments

We would like to thank Drs. F. Alt and C. Bassing for stimulating and helpful discussions. We also gratefully acknowledge Drs. E. Derudder and M. Alimzhanov for critical reading of the text. The Dana-Farber/Harvard Cancer Center DNA Sequencing Facility processed sequences in a timely and efficient manner.

This work was supported by National Institutes of Health grant no. P01 AI52343.

The authors have no conflicting financial interests.

Notes

S.B. Koralov and T.I. Novobrantseva contributed equally to this work.

T.I. Novobrantseva's present address is Biogen Idec, Inc., Cambridge, MA 02142.

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